共查询到20条相似文献,搜索用时 46 毫秒
1.
- (1)
- Behavioural regulation of body temperature (tb) was monitored in 23 free-ranging Bufo calamita (Bc) and 17 syntopic Bufo viridis (Bv) at Urmitz (Rhineland-Palatinate, Germany) using temperature-sensitive transmitters implanted to the abdominal cavity.
- (2)
- In field tb varied between +0.5 and 37.4 °C in Bc and between +0.6 and 33.7 °C in Bv. Maximum tb of a Bc measured during an experimental trial was 38.8 °C.
- (3)
- Natterjack toads avoided environmental temperature extremes by burrowing actively into moist sandy soil (2-90 cm deep), whereas green toads hid exclusively in mammal burrows or pre-existing subterranean cavities. Shelter choice did not vary between summer and winter.
- (4)
- Average tb of Bc exceeded significantly that of Bv during summer (26.7 °C vs. 24.7 °C), while the reverse was true during winter (4.2 °C vs. 7.2 °C). Following hibernation the body condition of Bv was significantly lower than that of Bc.
- (5)
- We conclude that green toads fail to colonise regions west of the Rhine valley because of a combination of winter temperatures impeding foraging trips for prolonged periods, the choice of warm hibernacula increasing metabolic costs and/or predation risk and reduced fecundity.
2.
Metabotropic glutamate receptor subtype 1a (mGluR1a) associates with the proteins mediating its receptor activity, suggesting a complex-controlled function of mGluR1a. Here, using glutathione-S-transferase pull-down, co-immnoprecipitation and immnoflurescence assays in vitro and in vivo, we have found CFTR-associated ligand (CAL) to be a novel binding partner of mGluR1a, through its PSD95/discslarge/ZO1homology domain. Deletion of mGluR1a-carboxyl terminus (CT) or mutation of Leu to Ala in the CT of mGluR1a reduces the association, indicating the essential binding region of mGluR1a for CAL. Functionally, the interaction of mGluR1a with CAL was shown to inhibit mGluR1a-mediated ERK1/2 activation, without an apparent effect, via the C-terminal-truncated receptor. These findings might provide a novel mechanism for the regulation of mGluR1a-mediated signaling through the interaction with CAL.
Structured summary
- MINT-6797987, MINT-6798009:
- NHERF-2 (uniprotkb:Q15599) binds (MI:0407) to mGluR1a (uniprotkb:Q9R0W0) by proteinarray (MI:0089)
- MINT-6798026, MINT-6798048, MINT-6798066:
- mGluR1a (uniprotkb:Q9R0W0) physically interacts (MI:0218) with CAL (uniprotkb:Q9HD26) by pull down (MI:0096)
- MINT-6797953, MINT-6797970:
- NHERF-1 (uniprotkb:O14745) binds (MI:0407) to mGluR1a (uniprotkb:Q9R0W0) by protein array (MI:0089)
- MINT-6797935:
- CAL (uniprotkb:Q9HD26) binds (MI:0407) to mGluR1a (uniprotkb:Q9R0W0) by protein array (MI:0089)
- MINT-6798084:
- CAL (uniprotkb:Q9HD26) binds (MI:0407) to mGluR1a (uniprotkb:Q9R0W0) by filter binding (MI:0049)
- MINT-6798134:
- mGluR1a (uniprotkb:Q9R0W0) physically interacts (MI:0218) with CAL (uniprotkb:Q9HD26) by anti tag coimmunoprecipitation (MI:0007)
- MINT-6798158:
- CAL (uniprotkb:B4F775) physically interacts (MI:0218) with mGluR1a (uniprotkb:Q9R0W0) by anti bait coimmunoprecipitation (MI:0006)
- MINT-6798233:
- CAL (uniprotkb:Q9HD26) colocalizes (MI:0403) with mGluR1a (uniprotkb:Q9R0W0) by fluorescence microscopy (MI:0416)
3.
Tavershima Dzenda Joseph O. AyoClarence A.M. Lakpini Alexander B. Adelaiye 《Journal of thermal biology》2011,36(5):255-263
- (1)
- Rectal temperature (Tr) was measured in captive African giant rats (Cricetomys gambianus, Waterhouse), live-trapped in the Savannah during the harmattan, hot-dry and rainy seasons with the aim of determining diurnal, seasonal and sex patterns.
- (2)
- Mean (±SEM) Tr in the morning (37.16±0.04 °C) was lower (P<0.001) than the afternoon (37.49±0.03 °C) and evening (37.66±0.03 °C). There was no significant difference (P>0.05) between afternoon and evening Tr during the harmattan and rainy seasons, but the difference was significant (P<0.001) during the hot-dry season. Overall Tr was higher (P<0.001) during the hot-dry (38.03±0.03 °C) than harmattan (37.17±0.03 °C) and rainy (37.21±0.03 °C) seasons. Tr of bucks was lower than that of does (P<0.0001) during the harmattan and rainy seasons, but sex difference during the hot-dry season was not significant (P>0.05).
- (3)
- Base-line Tr values for the African giant rats are shown for the first time. Season, time of day and sex influence fluctuations in Trs of African giant rats, and should be considered during diagnostic and clinical evaluations.
4.
- 1.
- Morphological and stereological changes were examined in the pituitary gland of the rats exposed to heat stress (38 °C) for 60 min. 相似文献
5.
- (1)
- Interactions between temperature and hypoxia in ectothermic tetrapods (reptiles and amphibians) are reviewed. 相似文献
6.
7.
8.
- •
- WHAT IS THE CORTEX?
- •
- The Egg Cortex
- •
- The Scope of This Review
- •
- STORING INFORMATION AND BUILDING THE CORTEX DURING OOGENESIS
- •
- Localizing Maternal Determinants in the Cortex
- •
- Structuring and Positioning Information in the Cortex
- •
- Cortical Polarity and Storage of Information in Other Oocytes
- •
- The Cortex during Vitellogenesis
- •
- MATURATION PREPARES THE CORTEX FOR FERTILIZATION
- •
- Restructuring Cortical Endoplasmic Reticulum and Organelles during Maturation
- •
- Restructuring the Cytoskeleton during Maturation
- •
- Relocalization of Cortical mRNAs and Domains during Maturation
- •
- The Cortex as the Site of Attachment and Fusion of Sperm and Egg
- •
- FERTILIZATION TRANSFORMS THE EGG CORTEX
- •
- Sperm-Induced Calcium Waves and Membranes
- •
- Waves of Exocytosis and Endocytosis at Fertilization
- •
- Fertilization-Induced Microfilament Reorganizations
- •
- The Fertilized Egg Cortex
- •
- WHAT THE CORTEX IS
- •
- The Cortex as a Repository for Spatiotemporal Information
- •
- The Questions That Remain
9.
- 1.
- Heat tolerance of dry cysts of the fairy shrimp Branchipus schaefferi and the brine shrimp, Artemia franciscana was studied using rapid (∼100 °C/min) and slow (∼4 °C/min) heating. 相似文献
10.
Jesper Givskov Sørensen Minna Pekkonen Beatrice Lindgren Volker Loeschcke Anssi Laurila Juha Merilä 《Journal of thermal biology》2009
- 1.
- We tested for geographical variation in heat tolerance and Hsp70 expression levels of Rana temporaria tadpoles along a 1500 km long latitudinal gradient in Sweden. 相似文献
11.
The transport protein particle (TRAPP) complex is required for proper vesicular transport from the ER to the Golgi. The composition of yeast TRAPP is well characterized, but the organization of mammalian TRAPP complex remains elusive. Using a tandem affinity purification (TAP) approach, we provide first experimental proof for the association of NIBP (NIK/IKKβ binding protein) with Bet3 and find two human paralogs of Trs33 (A and B) associated with Bet3. Interaction studies and gel filtration analysis reveal that both proteins are part of human TRAPP and might mark two distinct isocomplexes that exert different functions in the regulation of ER-to-Golgi traffic.
Structured summary
- MINT-6784845:
- Bet3 (uniprotkb:O43617) physically interacts (MI:0218) with Trs33B (uniprotkb:Q86SZ2) by anti bait coimmunoprecipitation (MI:0006)
- MINT-6785053:
- Trs33B (uniprotkb:Q86SZ2) physically interacts (MI:0218) with Bet3 (uniprotkb:O43617) and Sedl (uniprotkb:O14582) by anti bait coimmunoprecipitation (MI:0006)
- MINT-6784856:
- Bet3 (uniprotkb:O43617) physically interacts (MI:0218) with Trs33A2 (uniprotkb:O75865-2) by anti bait coimmunoprecipitation (MI:0006)
- MINT-6785038:
- Trs33A1 (uniprotkb:O75865-2) physically interacts (MI:0218) with Sedl (uniprotkb:O14582) and Bet3 (uniprotkb:O43617) by anti bait coimmunoprecipitation (MI:0006)
- MINT-6784879:
- Bet3 (uniprotkb:O43617) physically interacts (MI:0218) with NIBP (uniprotkb:Q96Q05) by tandem affinity purification (MI:0676)
- MINT-6785068:
- Trs33B (uniprotkb:Q86SZ2), Trs33A2 (uniprotkb:O75865-2) and Bet3 (uniprotkb:O43617) colocalize (MI:0403) by molecular sieving (MI:0071)
- MINT-6785415:
- Bet3 (uniprotkb:O43617) physically interacts (MI:0218) with Trs33A1 (uniprotkb:O75865) by anti bait coimmunoprecipitation (MI:0006)
12.
- ·
- Juvenile Chinook salmon Oncorhynchus tshawytscha survival and behaviour were evaluated during a temperature increase from 8.8 to 23.2 °C. 相似文献
13.
- 1.
- Thermoregulatory behavior of fed and fasted desert hamsters (Phodopus roborovskii) acclimated to summer- [16 light (L):8 dark (D), ambient temperature (Ta)=26.5 °C] and winter-like (8L:16D, Ta=10 °C) conditions was studied. Body temperature (Tb), selected temperature and activity were measured in hamsters placed in a thermal gradient system for 48 h. 相似文献
14.
- 1.
- Doppler-image ultrasonography was used to document vascular changes and blood flow rates of cattle (Bos taurus) under hot (32.7 °C) and cold (8.1 °C) conditions for 24 h. 相似文献
15.
- 1.
- We investigated whether heat tolerance has a crucial impact on the altitudinal distribution of a high-altitude lizard, Takydromus hsuehshanensis (>1800 m altitude). 相似文献
16.
- 1.
- Thirty-seven Peucetia viridans egg sacs were incubated at 15, 17.5, 20, 25, 30, 32.5 or 35 °C. Hatching time was inversely proportional to temperature, and no hatching occurred at the extremes (15, 17.5 and 35 °C). 相似文献
17.
Ildikó Nagy 《FEBS letters》2008,582(29):4003-4007
Cochlin is colocalized with type II collagen in the extracellular matrix of cochlea and has been suggested to interact with this collagen. Here we show that the second von Willebrand type A domain of cochlin has affinity for type II collagen, as well as type I and type IV collagens whereas the LCCL-domain of cochlin has no affinity for these proteins. The implications of these findings for the mechanism whereby cochlin mutations cause the dominant negative DFNA9-type hearing loss are discussed.
Structured summary
- MINT-6796048:
- type I collagen (uniprotkb:P02452) binds (MI:0407) to cochlin-vWA2 uniprotkb:O43405) by surface plasmon resonance (MI:0107)
- MINT-6796166:
- type III collagen (uniprotkb:P02462) binds (MI:0407) to cochlin-vWA2 (uniprotkb:O43405) by surface plasmon resonance (MI:0107)
- MINT-6796062:
- type II collagen (uniprotkb:P02458) binds (MI:0407) to cochlin-vWA2 (uniprotkb:O43405) by surface plasmon resonance (MI:0107)
18.
Shan SF Wang LF Zhai JW Qin Y Ouyang HF Kong YY Liu J Wang Y Xie YH 《FEBS letters》2008,582(27):3723-3728
Prospero-related homeobox protein (Prox1) plays essential roles in the development of many tissues and organs. In the present study, we show that Prox1 is modified by the small ubiquitin-like protein SUMO-1 in cultured cells. Mutation analysis identified at least four potential sumoylation sites within the repression domain of Prox1. Our data indicate that sumoylation of Prox1 reduces its interaction with HDAC3 and as a result downregulates its corepressor activity. These findings suggest that sumoylation may serve as a novel mechanism for the regulation of Prox1’s corepressor activity.
Structured summary
- MINT-6787569:
- PROX1 (uniprotkb:Q92786) physically interacts (MI:0218) with HDAC3 (uniprotkb:O15379) by anti tag coimmunoprecipitation (MI:0007)
- MINT-6787767:
- PROX1 (uniprotkb:Q92786) physically interacts (MI:0218) with SUMO-1 (uniprotkb:P63165) by anti tag coimmunoprecipitation (MI:0007)
19.
20.
- 1.
- Various factors affect a reptile's capacity for thermoregulation and most studies have focussed on terrestrial species.
- 2.
- We investigated the thermoregulatory abilities of the yellow anaconda (Eunectes notaeus) in terms of selected body temperature (Tsel), set-point range (Tset) and body posture in terrestrial and aquatic thermal mosaics.
- 3.
- Yellow anacondas selected higher body temperatures (Tb) and have a narrower Tset in a terrestrial environment than in an aquatic one.
- 4.
- Coiled body postures were most frequently observed and were generally associated with higher Tb.