IKKε regulates cell elongation through recycling endosome shuttling

1. Download : Download high-res image (412KB)
2. Download : Download full-size image

Highlights? Recycling endosomes shuttle along the proximal-distal axis of growing bristles ? IKK? locally antagonizes Rab11 effector Nuf at the bristle tip by phosphorylation ? Nuf-endosome pathway is independent of actin cytoskeleton or DIAP1-caspase pathway ? Mammalian IKK-related kinases antagonize Rab11-FIP3 through phosphorylation     

Loss of Rb Cooperates with Ras to Drive Oncogenic Growth in Mammalian Cells

1. Download : Download high-res image (202KB)
2. Download : Download full-size image

Highlights? Oncogenic Ras drives weak growth through the PI3K canonical growth pathway ? A novel E2F-independent role for the Rb family in cell biogenesis ? Ras and loss of Rb synergize to give robust and sustained cell growth ? PI3K pathway is crucial for sustained growth, acting to maintain nutrient uptake     

A comprehensive genomic binding map of gene and chromatin regulatory proteins in Saccharomyces

1. Download : Download high-res image (328KB)
2. Download : Download full-size image

Highlights? Genomic maps of 200 yeast regulators in normal and stress conditions provide a compendium for discovery ? SAGA assembly pathway is associated with more regulation than the TFIID pathway ? SAGA-linked regulators are distal, and TFIID-linked regulators are proximal to the promoter ? During heat shock, SAGA- and TFIID-linked regulators displayed distinct mobilization patterns     

Allosteric Mechanism Controls Traffic in the Chaperone/Usher Pathway

1. Download : Download high-res image (229KB)
2. Download : Download full-size image

Highlights? Allosteric mechanism controls traffic in the chaperone/usher pathway ? Usher-binding site in free chaperone is conformationally locked ? Subunit binding opens the lock in the chaperone, allowing usher targeting     

Saturated fatty acid and TLR signaling link β cell dysfunction and islet inflammation

1. Download : Download high-res image (196KB)
2. Download : Download full-size image

Highlights? Palmitate induces β cell dysfunction by activating inflammatory processes in islets ? β cells sense palmitate via the TLR4 pathway and recruit M1 macrophages to islets ? M1 macrophages play a pivotal role in palmitate-induced β cell dysfunction ? M1 macrophages and inflammation also play a role in β cell dysfunction in T2D models     

The MRN-CtIP Pathway Is Required for Metaphase Chromosome Alignment

1. Download : Download high-res image (178KB)
2. Download : Download full-size image

Highlights? The MRN-CtIP pathway is required for metaphase chromosome alignment in egg extracts ? MRN inhibition interferes with spindle assembly around DNA-coated beads ? MRN inhibition in cells causes a metaphase delay and disrupts the RanGTP gradient ? The MRN complex regulates the stable association of RCC1 to chromatin     

O-GlcNAc Signaling Entrains the Circadian Clock by Inhibiting BMAL1/CLOCK Ubiquitination

1. Download : Download high-res image (176KB)
2. Download : Download full-size image

Highlights? The hexosamine/O-GlcNAc pathway regulates cellular clock oscillation ? OGT promotes expression of BMAL1/CLOCK target genes in cultured cells and liver ? Rhythmic O-GlcNAcylation stabilizes BMAL1 and CLOCK by inhibiting their ubiquitination ? Hepatic manipulation of OGT perturbs the diurnal rhythm of glucose homeostasis     

Structural Characterization of a Mouse Ortholog of Human NEIL3 with a Marked Preference for Single-Stranded DNA

1. Download : Download high-res image (422KB)
2. Download : Download full-size image

Highlights? Neil3 is an Fpg/Nei DNA glycosylase of the base excision repair pathway ? Neil3 excises a broad spectrum of base lesions, with a marked preference for ssDNA ? We report a crystal structure of Neil3 DNA glycosylase ? The structure provides valuable insights into Neil3′s unusual substrate specificity     

Noncanonical Role of the 9-1-1 Clamp in the Error-Free DNA Damage Tolerance Pathway

1. Download : Download high-res image (310KB)
2. Download : Download full-size image

Highlights? 9-1-1 and Exo1 are components of the error-free RAD6 pathway ? 9-1-1 promotes postreplicative template switching ? Polyubiquitylated PCNA and 9-1-1 cooperate in the error-free RAD6 pathway ? 9-1-1’s role in the error-free RAD6 pathway is uncoupled from checkpoint functions     

Early Folding Events Protect Aggregation-Prone Regions of a β-Rich Protein

1. Download : Download high-res image (163KB)
2. Download : Download full-size image

Highlights? The folding pathway of a β-barrel protein protects it from aggregation ? The rate-determining transition state of CRABP1 is polarized and malleable ? Regions constituting the aggregate core of CRABP1 are protected early in folding ? Early barrel closure in iLBPs may offer a general strategy for productive folding     

Axon Regrowth during Development and Regeneration Following Injury Share Molecular Mechanisms

1. Download : Download high-res image (146KB)
2. Download : Download full-size image

Highlights? Initial axon outgrowth and regrowth during remodeling are mechanistically distinct ? The nuclear receptor UNF is necessary for axon regrowth but not for initial growth ? TOR is also required for developmental regrowth but not for initial axon outgrowth ? UNF promotes axon regrowth via the TOR pathway and guidance via unknown mechanisms     

Local zones of endoplasmic reticulum complexity confine cargo in neuronal dendrites

1. Download : Download high-res image (227KB)
2. Download : Download full-size image

Highlights? The endoplasmic reticulum (ER) in dendrites shows spatial variation in complexity ? Increased complexity occurs at dendritic branch points, confining membrane cargo ? An mGluR/PKC/CLIMP63 pathway bidirectionally regulates ER complexity ? ER complexity controls dendritic morphogenesis and defines sites of branch formation     

Mycobacterium tuberculosis activates the DNA-dependent cytosolic surveillance pathway within macrophages

1. Download : Download high-res image (154KB)
2. Download : Download full-size image

Highlights? M. tuberculosis permeabilizes phagosomes and activates cytosolic signaling pathways ? Host cytoplasmic DNA receptors sense M. tuberculosis extracellular DNA ? DNA recognition activates the STING/TBK1/IRF3 pathway ? Cytosolic sensing promotes M. tuberculosis infection     

Robustness and Epistasis in the C. elegans Vulval Signaling Network Revealed by Pathway Dosage Modulation

1. Download : Download high-res image (275KB)
2. Download : Download full-size image

Highlights? A phase map of cell fate patterns is built as a function of signaling pathway dose ? The vulva system can buffer a 4-fold variation in mean lin-3/egf mRNA number ? The major role of LIN-12/Notch in the vulva is to promote the 2° fate ? Inhibition of 1° fate by LIN-12 is important when lin-3 dose is mildly increased     

SIP1 Mediates Cell-Fate Decisions between Neuroectoderm and Mesendoderm in Human Pluripotent Stem Cells

1. Download : Download high-res image (184KB)
2. Download : Download full-size image

Highlights? Smad-interacting protein 1 (SIP1) regulates hESC differentiation ? SIP1 upregulation promotes neuroectodermal differentiation ? SIP1 inhibits mesendodermal and endodermal differentiation ? SMAD2/3 and NANOG/OCT4/SOX2 cooperatively regulate SIP1 expression     

Keeping cell identity in Arabidopsis requires PRC1 RING-finger homologs that catalyze H2A monoubiquitination

1. Download : Download high-res image (139KB)
2. Download : Download full-size image

Highlights? AtBMI1A/B proteins prevent misexpression of embryonic traits in somatic cells ? PRC2 and AtBMI1A/B proteins maintain cells in their differentiated state ? AtBMI1A/B proteins mediate H2A monoubiquitination