Basic versus applied research: Julius Sachs (1832–1897) and the experimental physiology of plants

The German biologist Julius Sachs was the first to introduce controlled, accurate, quantitative experimentation into the botanical sciences, and is regarded as the founder of modern plant physiology. His seminal monograph Experimental-Physiologie der Pflanzen (Experimental Physiology of Plants) was published 150 y ago (1865), when Sachs was employed as a lecturer at the Agricultural Academy in Poppelsdorf/Bonn (now part of the University). This book marks the beginning of a new era of basic and applied plant science. In this contribution, I summarize the achievements of Sachs and outline his lasting legacy. In addition, I show that Sachs was one of the first biologists who integrated bacteria, which he considered to be descendants of fungi, into the botanical sciences and discussed their interaction with land plants (degradation of wood etc.). This “plant-microbe-view” of green organisms was extended and elaborated by the laboratory botanist Wilhelm Pfeffer (1845–1920), so that the term “Sachs-Pfeffer-Principle of Experimental Plant Research” appears to be appropriate to characterize this novel way of performing scientific studies on green, photoautotrophic organisms (embryophytes, algae, cyanobacteria).     

Theophile Chudzinski (1840–1897)

Biographies of European Anthropologists by D. Ferembach

Theophile Chudzinski (1840–1897)     

Measurement Properties of the Chinese Version of the Youth Quality of Life Instrument–Weight Module (YQOL-W)

Background

Childhood obesity is a growing public health concern in China. It not only compromises physical health, but also has negative impacts on psychosocial well-being. As obesity rates increase, finding out what the perceptions of Chinese youth are regarding their weight is important for intervention planning and evaluation. However, there is a paucity of available obesity-specific instruments for children and adolescents in China and youth weight-specific quality of life (QOL) has been little reported. This study aimed to evaluate the measurement properties of the Chinese version of the Youth Quality of Life Instrument – Weight Module (YQOL-W).

Methods

The Chinese version of the YQOL-W was administered to 840 youth aged 11–18 from nine schools. Measurement properties including measurement model, reliability, validity and burden were evaluated.

Results

Confirmatory factor analysis showed that a three-factor model had acceptable model fit. The instrument had robust internal consistency reliability with Cronbach''s α ranging from 0.84 to 0.96 and acceptable test-retest reliability with the intraclass correlation coefficients (ICCs) all higher than 0.7. The standard error of measurement (SEM) values for the Self, Social and Environment factors and total score were 10.352, 9.526, 12.086 and 8.425, respectively. The small real differences (SRDs) for the Self, Social and Environment factors and total score were 28.675, 26.387, 33.478, and 23.337, respectively. The Pearson''s correlation coefficients between the YQOL-W and the PedsQL4.0 General Core Scales were stronger between comparable dimensions than those between less comparable dimensions, demonstrating convergent and discriminant evidence of construct validity. Significant differences were found in subscale and total scores across weight status, age and genders (P<0.01), supporting the known-groups validity of the instrument.

Conclusion

The Chinese version of the YQOL-W has acceptable measurement properties and can be used to assess the weight-specific QOL of children and adolescents in China.     

Championing Ecosystem Sustainability and Health: Profile and Tribute to the Life and Work of James Kay (1954–2004)

The past decade has seen considerable developments in the integrated study of ecosystem sustainability and health. Important developments in theory, methods, and application of this area have emerged from the work of key individuals and informal, multidisciplinary networks of peers working across continents and countries and based in governments, universities, and private organizations. This profile focuses in particular on the critical influence of James Kay as a key advocate and intellectual champion for incorporating complexity and uncertainty into the Ecosystem approach. The intent is to provide an overview of an important era in the application of this approach to address health and sustainability concerns and to highlight the frameworks, methods, and networks that have emerged as collective acknowledgments to the life and work of James Kay (1954–2004).     

The Birth and Life of Species–Cultures

Evolution and life phenomena can be understood as results of history, i.e., as outcomes of cohabitation and collective memory of populations of autonomous entities (individuals) across many generations and vast extent of time. Hence, evolution of distinct lineages of life can be considered as isomorphic with that of cultures. I argue here that cultures and culture-like systems – human culture, natural languages, and life forms – always draw from history, memory, experience, internal dynamics, etc., transforming themselves creatively into new patterns, never foreseen before. This is possible thanks to the fact that all forms of life are descendants of life. Ontogeny and speciation in various lineages draw from continuous re-interpretation of conservative genetic/generic “texts”, as well as from changes of the interpretative process itself. The result is continuous appearances of new lineages-cultures and/or communities-cultures, in a semiotic process of re-interpretation and inventing new ways of living. The topic is developed here on the backgrounds of ideas presented by R. A. Rappaport in “Ritual and religion in the making of humanity” and J. Flegr in “Frozen evolution”.     

Biological Discourses on Human Races and Scientific Racism in Brazil (1832–1911)

This paper analyzes biological and scientific discourses about the racial composition of the Brazilian population, between 1832 and 1911. The first of these dates represents Darwin’s first arrival in the South-American country during his voyage on H.M.S. Beagle. The study ends in 1911, with the celebration of the First universal Races congress in London, where the Brazilian physical anthropologist J.B. Lacerda predicted the complete extinction of black Brazilians by the year 2012. Contemporary European and North-American racial theories had a profound influence in Brazilian scientific debates on race and miscegenation. These debates also reflected a wider political and cultural concern, shared by most Brazilian scholars, about the future of the Nation. With few known exceptions, Brazilian evolutionists, medical doctors, physical anthropologists, and naturalists, considered that the racial composition of the population was a handicap to the commonly shared nationalistic goal of creating a modern and progressive Brazilian Republic.     

Pavel Siffel (1954–2003) or Life full of chlorophyll

Life and research results of Pavel Siffel, a talented but untimely deceased Czech scientist in photosynthesis, are reviewed. He studied biophysics and physiology of chlorophyll, its complexes with proteins, their absorption and fluorescence spectra, activities in mutants and transformants, dealt with chlorophyll biosynthesis and protochlorophyllide photoreduction, pigments in plants grown at CO₂ deficiency and under simulated acid rain, with changes accompanying leaf and plant development, photobleaching, etc. He participated in construction of specialised spectrofluorometers, finally he built the kinetic spectrophotometer SpeKin.     

Agglutinating Activity and Structural Characterization of Scalarin,the Major Egg Protein of the Snail Pomacea scalaris (d’Orbigny, 1832)

Apple snail perivitellins are emerging as ecologically important reproductive proteins. To elucidate if the protective functions of the egg proteins of Pomacea canaliculata (Caenogastropoda, Ampullariidae), involved in embryo defenses, are present in other Pomacea species we studied scalarin (PsSC), the major perivitellin of Pomacea scalaris. Using small angle X-ray scattering, fluorescence and absorption spectroscopy and biochemical methods, we analyzed PsSC structural stability, agglutinating activity, sugar specificity and protease resistance. PsSC aggluttinated rabbit, and, to a lesser extent, human B and A erythrocytes independently of divalent metals Ca²⁺ and Mg²⁺ were strongly inhibited by galactosamine and glucosamine. The protein was structurally stable between pH 2.0 to 10.0, though agglutination occurred only between pH 4.0 to 8.0 (maximum activity at pH 7.0). The agglutinating activity was conserved up to 60°C and completely lost above 80°C, in agreement with the structural thermal stability of the protein (up to 60°C). PsSC was able to withstand in vitro gastrointestinal digestion, and showed no trypsin inhibition activity. The presence of lectin activity has been reported in eggs of other Pomacea snails, but here we link for the first time, this activity to an apple snail multifunctional perivitellin. This novel role for a snail egg storage protein is different from closely related P.canaliculata defensive proteins.     

Demonstration of cross-reacting material in Tay–Sachs disease

Antibodies against placental hexosaminidase A and kidney alpha-subunits were raised in rabbits after cross-linking the antigens with glutaraldehyde. Anti-(alpha(n)-subunit) antiserum (anti-alpha(n)) precipitated hexosaminidase A but not hexosaminidase B, whereas anti-(hexosaminidase A) antiserum precipitated both hexosaminidases A and B. Specific anti-(hexosaminidase A) antiserum was prepared by absorbing antiserum with hexosaminidase B. Both anti-alpha(n) and anti-(hexosaminidase A) antisera precipitated the CR (cross-reacting) material from eight unrelated patients with Tay-Sachs disease. Immunotitration, immunoelectrophoresis, double-immunodiffusion and radial-immunodiffusion techniques were used to demonstrate the presence of CR material. The CR-material-antibody complex was enzymically inactive. Antiserum raised against kidney or placental hexosaminidase A, without cross-linking with glutaraldehyde, failed to precipitate the CR material, implying that treatment of the protein with glutaraldehyde exposes antigenic determinants that are hidden in the native protein. Since anti-(hexosaminidase B) antiserum did not precipitate the CR material during the immunoelectrophoresis of Tay-Sachs liver extracts, it is suggested that altered alpha-subunits do not combine with beta-subunits. By using immunotitration we have demonstrated the competition between the hexosaminidase B-free Tay-Sachs liver extract and hexosaminidase A for the common binding sites on monospecific anti-(cross-linked hexosaminidase A) antiserum. The amount of CR material in the liver samples from seven cases of Tay-Sachs desease was found to be in the same range as theoretically calculated alpha-subunits in normal liver samples. Similar results were obtained by the radial-immunodiffusion studies. The present studies therefore suggest that Tay-Sachs disease is caused by a structural-gene mutation.     

Life Science:Innovation and prosperity—commemorating the 60~(th) anniversary of the Chinese Academy of Sciences

<正>In tandem with the birth and growth of the People's Republic of China (PRC),the Chinese Academy of Sciences (CAS) passed through six decades by the year of 2009.     

The Yin–Yang of Dendrite Morphology: Unity of Actin and Microtubules

Actin and microtubules (MT) are targets of numerous molecular pathways that control neurite outgrowth. To generate a neuronal protrusion, coordinated structural changes of the actin and MT cytoskeletons must occur. Neurite formation occurs when actin filaments (F-actin) are destabilized, filopodia are extended, and MTs invade filopodia. This process results in either axon or dendrite formation. Axonal branching involves interplay between F-actin and MTs, with F-actin and MTs influencing polymerization, stabilization, and maintenance of each other. Our knowledge of the mechanisms regulating development of the axon, however, far eclipses our understanding of dendritic development and branching. The two classes of neurites, while fundamentally similar in their ability to elongate and branch, dramatically differ in growth rate, orientation of polarized MT bundles, and mechanisms that initiate branching. In this review, we focus on how F-actin, MTs, and proteins that link the two cytoskeletons coordinate to specifically initiate dendritic events. Penelope C. Georges and Norell M. Hadzimichalis contributed equally.     

Effects of Endophyte Colonization of Vicia faba (Fabaceae) Plants on the Life–History of Leafminer Parasitoids Phaedrotoma scabriventris (Hymenoptera: Braconidae) and Diglyphus isaea (Hymenoptera: Eulophidae)

Effects of the fungal endophytes Beauveria bassiana (isolates ICIPE 279, G1LU3, S4SU1) and Hypocrea lixii (isolate F3ST1) on the life-history of Phaedrotoma scabriventris and Diglyphus isaea, parasitoids of the pea leafminer Liriomyza huidobrensis, were studied in the laboratory. Parasitoids were allowed to parasitize 2^nd and 3^rd instar L. huidobrensis larvae reared on endophytically-inoculated faba bean, Vicia faba. In the control, parasitoids were reared on non-inoculated host plants. Parasitism, pupation, adult emergence and survival were recorded. No significant difference was observed between the control and the endophyte-inoculated plants in terms of parasitism rates of P. scabriventris (p = 0.68) and D. isaea (p = 0.45) and adult'' survival times (p = 0.06). The survival period of the F1 progeny of P. scabriventris was reduced (p<0.0001) in B. bassiana S4SU1 to 28 days as compared to more than 40 days for B. bassiana G1LU3, ICIPE 279 and H. lixii F3ST1. However, no significant difference (p = 0.54) was observed in the survival times of the F1 progeny of D. isaea. This study has demonstrated that together, endophytes and parasitoids have beneficial effects in L. huidobrensis population suppression.     

Barremian–Aptian Assemblages of Microfauna and Palynomorphs from the North-West of the Yamal Peninsula (Biostratigraphy and Palaeofacies)

Paleontological Journal - The composition of Barremian–Aptian assemblages of microfauna and palynomorphs from the north-west of the Yamal Peninsula allows to reveal local zones, which are...     

Adriano Buzzati-Traverso and the foundation of the International Laboratory of Genetics and Biophysics in Naples (1962–1969)

Despite a long tradition of research in applied genetics, particularly in agricultural research, in Italy the transition to the new knowledges and techniques of molecular biology was long and difficult. Political and financial constraints made academic institutions very slow to grasp the importance of molecular approaches to biology and medicine. In fact, the main studies concerning problems of molecular biology took place inside non-academic institutions. We reconstruct the complex paths leading to the birth of the International Laboratory of Genetics and Biophysics (LIGB) in Naples, and describe its work and activities in the period in which it was directed by its creator, Adriano Buzzati-Traverso, between 1962 and 1969. The origins of the LIGB are inextricably bound to the growth of biophysical research at the University of Pavia and at the Higher Health Institute in Rome. For a short period, with the aid provided by Italian and European physicists to biological research on the effects of nuclear radiation, LIGB became a focal point in European molecular biology. In 1968 the scientific and educational activities of the LIGB dramatically fell victim to the contradictions of the Italian academic and scientific system, and to the political climate which emerged in Italy at the time.