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The structure of a transcriptional unit on colicin E1 plasmid. 总被引:26,自引:0,他引:26
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Characterization of in vitro transcription initiation and termination sites in Col E1 DNA. 总被引:15,自引:5,他引:10 下载免费PDF全文
R K Patient 《Nucleic acids research》1979,6(8):2647-2665
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The nucleotide sequence of 570 bp, covering the N-terminal portion of the colicin E1 gene, was determined. The sequence of the N-terminal four amino acids of the colicin E1 protein, determined by manual Edman degradation, agreed with that predicted from the nucleotide sequence. From analysis of the 5'-terminal sequences of RNAs synthesized in vitro, the promoter and operator regions of the colicin E1 gene were assigned. These data indicate the existence of two promoters, one of which is located in the coding region for colicin E1. DNA sequence homology of 16 bp was found between the putative operator regions of the colicin E1 and recA genes. 相似文献
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Tandem promoters direct E. coli ribosomal RNA synthesis. 总被引:46,自引:0,他引:46
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