共查询到20条相似文献,搜索用时 31 毫秒
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Wang Minqin Zhao Junsheng Peng Zhenying Guo Wei Wang Yun Wang Le Xia Guangmin 《Plant Cell, Tissue and Organ Culture》2008,92(2):121-130
In order to investigate chromosome elimination in symmetric somatic hybridization between Bupleurum scorzonerifolium and Arabidopsis thaliana, protoplasts were isolated from suspension cultures of both A. thaliana and B. scorzonerifolium parents. Biparental protoplasts were mixed at a rate of 1.5:1 and fused with PEG-method. After protoplast fusion, the products
were cultured in the P5 liquid medium for microcallus formation. Single cell lines formed from microcalli after subculturing
on the MB1 (Xia and Chen, Plant Sci 120:197–203, 1996) solid medium. The putative somatic hybrid cell lines were identified
by cytological and molecular analysis. Of the 132 somatic cell lines generated, 16 were identified as somatic hybrids, with
the phenotypes resembled B. scorzonerifolium parent. These hybrids showed a complete set of B. scorzonerifolium chromosome and 0–2 small chromosome(s) of A. thaliana. A few of them showed nuclear and cytoplasmic SSR fragments of A. thaliana. These hybrid cell lines could differentiate to green spots, buds/leaves through complementation of regeneration ability.
The chromosomes elimination of A. thaliana was discussed.
Wang Minqin and Zhao Junsheng contributed equally to the work. 相似文献
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Tarun Kumar Verinder Wahla Piyush Pandey R. C. Dubey D. K. Maheshwari 《World journal of microbiology & biotechnology》2009,25(2):277-285
Biological control of the cyst forming nematode Heterodera cajani was studied on sesame using plant growth promoting rhizobacteria (PGPR) Pseudomonas aeruginosa LPT3 and LPT5. Based on plant growth promoting attributes, two fluorescent pseudomonads, LPT3 and LPT5 were evaluated for
their efficacy against cyst forming nematode Heterodera cajani that parasitize Sesamum indicum. Pseudomonas aeruginosa LPT5 produced IAA, HCN, chitinase, glucanase and siderophore, and also solubilized inorganic phosphate in vitro. Moreover,
LPT5 resulted in mortality of second stage juveniles of H. cajani, which was 13% higher as compared to P. aeruginosa LPT3. Interestingly, when both strains were inoculated together for the management of H. cajani on Sesamum indicum the population of H. cajani was reduced significantly, in field trial. Approximately 60% reduction in cyst and juveniles population was recorded with
LPT5 coated seeds, while LPT3 resulted in 49% reduction in cyst and juvenile population as compared to control. Plants grown
with seeds bacterized with LPT5 and reduced doses of urea, diammonium phosphate (DAP), muriate of potash (K) and gypsum gave
maximum increase in yield, in comparison to that of plants raised under the influence of recommended or full doses of the
chemical fertilizers. Pseudomonas aeruginosa LPT5 also showed excellent root colonization. 相似文献
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We determined the incidence of curculionid beetles of the genus Lonchophorus on aborted and not aborted flower buds and developing fruits of the tree Ceiba pentandra in southeastern Costa Rica. Beetle larvae were found in reproductive parts of all trees sampled trees. The frequency of beetle
larvae was greater in aborted buds and immature fruits. A positive correlation between larvae development and flower bud development
indicates that female oviposition occurred in an early flower developmental stage and time until bud abortion is variable.
Weevil herbivory could be considered as one of the main factors that cause flower bud and fruit abortion in C. pentandra.
Resumen Determinamos la incidencia de curculiónidos del género Lonchophorus en botones florales y frutos inmaduros tanto abortados como no abortados en el árbol Ceiba pentandra en el SE de Costa Rica. Las larvas de curculiónidos fueron encontradas en todos los árboles muestreados. La frecuencia de larvas fue mayor en botones y frutos abortados. Una correlación positiva entre el desarrollo larval y el desarrollo del botón indica que la oviposición ocurre en una fase temprana de este y que el tiempo hasta el aborto es variable. La herbivoría por larvas de curculiónidos puede ser uno de los factores más importantes en el aborto de botones y frutos inmaduros en C. pentandra.相似文献
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The structure of a new pelomyxa species was investigated on the level fo light and electron microscopy. The length of locomotive
forms of Pelomyxa stagnalis reaches 800 μm. The thin layer of amorphous glycocalyx is located on the cell surface. Numerous nonfunctioning flagellae
are revealed predominantly in the uroidal zone. The axoneme has a nonstable set of microtubules. No additional structures
are present in the transition zone. The length of P. stagnalis flagella kinetosomes does not exceed 150 nm. Fifteen to twenty microtubules extend from the side surface of each kinetosome
at a small angle to the cell surface. One of main components of the P. stagnalis cytoplasm are structural vacuoles. Glycogen bodies in cells are surrounded by flattened ER cisterns, which are often filled
with electron-dense material. Cells of P. stagnalis were found to contain two species of prokaryote endobionts that differ in the peculiarities of their fine structure. The
number of nuclei in cells of the P. stagnalis adult individuals can reach 50 or more. The nuclei are surrounded by a bilayer envelope formed by the multilaminar layer
and by the outer layer composed of vesicles often filled with an electron-dense material. The nucleolus is usually single
and is located in the center of the nucleus. In nuclei, predominantly in connection with nucleoli, bodies are formed that
are formed by interlacing electron-dense strands. 相似文献
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Uredo behnickiana (syn. Hemileia americana and H. oncidii) is known on orchids of the genera Cattleya, Epidendrum, Laelia, Lophiaris and Oncidium. Previously, only the uredinial stages of these fungi have been described. The telial stage of the rust species was found
for the first time on Oncidium marshallianum. Because of a unique combination of morphological characters the fungus could not be assigned to Hemileia or any other known rust genus. Desmosorus is proposed, therefore, as a new genus with Desmosorus oncidii as the single known species. Desmosorus is characterised by (1) suprastomatal sori on which the spores are formed at the ends of branched hyphae, by (2) subglobose
to ovoid, echinulate urediniospores with a smooth patch around the hilum and by (3) thin-walled, oblong elliptical to cylindrical
teliospores with a rounded apex. 相似文献
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R. Ramirez-Malagon I. Aguilar-Ramirez A. Borodanenko L. Perez-Moreno J. L. Barrera-Guerra H. G. Nuñez-Palenius N. Ochoa-Alejo 《In vitro cellular & developmental biology. Plant》2007,43(6):660-665
Mammillaria species are the most numerous within Cactaceae family, and some of them are threatened with extinction as a result of human
activities. In this work, results of in vitro propagation are presented for ten Mammillaria species, testing 20 combinations of indole-3-acetic acid (IAA) and kinetin. Best results on shoot formation were obtained
using kinetin at two levels: 27.9 and 46.5 μM. All IAA levels tested were able to induce de novo shoot formation in M. bocasana, M. densispina, M. hahniana, M. hutchisoniana, M. orcutii, M. pectinifera, M. perbella, M. picta, M. rhodantha, and M. zephyranthoides. Depending on the IAA level tested, four responding groups were observed concerning their highest shoot-formation number.
For all species, the highest average of shoot formation was achieved with 5.7:46.5 or 11.4:46.5 μM IAA/kinetin, yielding 4.8
and 4.7 shoots per explant, respectively, in 60 d. Rooting of regenerated shoots was achieved by leaving the explants in their
shoot-induction medium or transferring them to half-strength MS medium. Hardening of regenerated plants was successfully achieved
by planting them in peat moss substrate after a desiccation treatment at room temperature for 3 d. 相似文献
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Ineke van Gremberghe Pieter Vanormelingen Katleen Van der Gucht Antoniya Mancheva Sofie D’hondt Luc De Meester Wim Vyverman 《Hydrobiologia》2009,635(1):147-155
We conducted a laboratory experiment to investigate the influence of Daphnia infochemicals on growth rate, microcystin production, colony formation and cell size of eight Microcystis strains isolated from two lakes. The strains were characterized genetically by their 16S-23S rDNA ITS sequence. The experiment
was composed of four treatments: (1) a control using filtered WC medium, (2) addition of Scenedesmus obliquus culture medium filtrate, (3) addition of Daphnia magna culture medium filtrate and (4) addition of sodium octyl sulphate, a commercially available Daphnia infochemical. Our results showed that sympatric strains differed strongly for the measured functional traits, while no correlations
between traits were found. Between-strain differences in growth rate, microcystin production, colony formation and cell size
were generally larger than the differences in phenotypes observed between treatments. Despite this, several strains reacted
to the infochemicals by changing functional trait values. Daphnia culture medium filtrate and, to a lesser extent, sodium octyl sulphate had a negative influence on the growth rate of half
of the strains and stimulated microcystin production in one strain, but the latter effect was not Daphnia-specific as Scenedesmus culture medium filtrate had the same effect. Daphnia culture medium filtrate also induced colony formation in one strain. Our data suggest that Daphnia infochemicals generally have a weak influence on growth rate, microcystin production and colony formation of Microcystis strains as compared to the inter-strain variability, while existing inducible effects are highly strain-specific. 相似文献
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An autolysin gene, atlh, was identified and sequenced from Streptococcus downei MFe28 using degenerate polymerase chain reaction (PCR) and the gene-walking method. Atlh protein encoded by atlh is composed of 879 amino acids, with a molecular weight of 95,902.26. Atlh possesses four 15-amino-acid residue repeats in
the putative cell-wall-binding domain and has a catalytic domain in the C-terminus. The deduced amino acid sequence of atlh showed homology to S. mutans autolysin AtlA (68.4% similarity). Inactivation of atlh resulted in elongated chain formation compared to the parent strain. Recombinant proteins Atlh and its derivatives were constructed
and analyzed by zymography. Zymographic analysis revealed that the Asp-771 residue of Atlh was essential for lytic activity
and that lytic activity was not diminished by the deletion of repetitive regions in the putative cell-wall-binding domain
of Atlh. Biofilm assay showed that the wild-type strain formed glucose- and sucrose-dependent biofilms, the atlh mutant diminished this ability. These results suggest that Atlh is associated with cell separation and biofilm formation. 相似文献
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Gaye Öngen Gaye Güngör Bahar Kanberoglu 《World journal of microbiology & biotechnology》2007,23(4):519-524
Aspergillus section Nigri strains Aspergillus aculeatus Ege-K 258, A.
foeditus var. pallidus Ege-K156, A. niger Ege-K 4 and A. tubingensis Ege-K 265 were used to treat olive mill wastewater (OMW) in an investigation aimed at exploring their dephenolisation and
decolourisation ability and, consequently, the economic feasibility of using any or all of these strains in a pre-treatment
step in the processing of OMW. Of these strains A. tubingensis Ege-K 265 resulted in an 80% decolourisation of twofold-diluted OMW and a 30% decolourisation of undiluted OMW; in addition,
it was able to remove approximately 30% of all phenolic compounds in both twofold-diluted and undiluted OMW. We conclude that
A. tubingensis Ege-K 265 could be effectively used in the pre-treatment step of a combined aerobic-anaerobic process to solve the environmental
problems caused by OMW in Mediterranean countries. 相似文献
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N. Yu. Markelova 《Microbiology》2010,79(6):777-779
Interaction of Bdellovibrio bacteriovorus 100NCJB with bacteria Campylobacter jejuni (strains 1, 2, 3, 4, and 5) and Helicobacter pylori, strain TX30a, was confirmed. The results indicate that lytic activity of bdellovibrios both in liquid media and cells attached
to a surface was observed. The potential use of the antimicrobial activity of predatory bacteria for environmental bioprotection
and public health is discussed. 相似文献
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Eustáquio Souza Dias Cláudia Regina Gontijo Labory Karina Marjorie Silva Herrera Alexandre Alonso Alves Giovana Augusta Torres Danny Lee Rinker 《World journal of microbiology & biotechnology》2008,24(11):2473-2479
Agaricus brasiliensis is a medicinal mushroom native to Brazil. It was first identified as Agaricus blazei and its scientific name continues to be debated. We examined the cytology of different Brazilian commercial strains of A. brasiliensis and the nuclear behavior of strain CS1 during basidiospore development using fluorescent microscopy. All strains have multinucleate
hyphae and no significant differences in nuclei numbers were observed between them. Basidia from A. brasiliensis strain CS1 are typically tetrasporics and produce binucleate basidiospores, demonstrating that a postmeiotic mitosis occurs
during basidiospore development. This result suggests that A. brasiliensis is primarily a heterothallic species. 相似文献
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The Dictyostelium discoideum homolog of mammalian cyclin dependent kinase 5 (Cdk5) has previously been shown to be required for optimal growth and differentiation
in this model organism, however, the subcellular localization of the protein has not previously been studied. In this study,
immunolocalizations and a GFP fusion construct localized Cdk5 predominantly to the nucleus of vegetative cells. Western blots
showed that Cdk5 was present in both nuclear and non-nuclear fractions, suggesting a functional role in both cellular locales.
During the early stages of mitosis, Cdk5 gradually moved from a punctate nucleoplasmic distribution to localize adjacent to
the inner nuclear envelope. During anaphase and telophase, Cdk5 localized to the cytoplasm and was not detected in the nucleoplasm.
Cdk5 returned to the nucleus during cytokinesis. Proteolytic activity has been shown to be a critical regulator of the cell
cycle. Immunoprecipitations coupled with immunolocalizations identified puromycin-sensitive aminopeptidase A (PsaA) as a potential
Cdk5 binding partner in Dictyostelium. Immunoprecipitations also identified two phosphotyrosine proteins (35 and 18 kDa) that may interact with Cdk5 in vivo. Together,
this work provides new insight into the localization of Cdk5, its function during cell division, and its binding to a proteolytic
enzyme in Dictyostelium. 相似文献
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O. S. Fedyanina 《Russian Journal of Genetics》2010,46(3):293-299
Tubulin-folding cofactor D is necessary for the assembly of tubulin heterodimers and, possibly, plays additional roles in
the cell. The effects of cofactor D, microtubules, and/or tubulin dimers on the mitosis initiation were studied in Schizosaccharomyces pombe. It was found for the first time that S. pombe cells with the alp1-1315 and cdc25-22 mutations remained highly viable at 36°C for 8 h, in contrast to cells with the alp1-1315 mutation alone. The progression of cdc25-22 alp1-1315 cells through mitosis after a cell division arrest at 36°C was described. When transferred to 25°C, cdc25-22 alp1-1315 cells displayed a lag of approximately 30 min in Plo1-GFP appearance in the spindle pole body (SPB), 1 h in chromosome condensation,
and 75 min in spindle formation. Thus, the initiation of mitosis in cdc25-22 alp1-1315 cells was delayed as compared with cdc25-22 cells. Since treatment of cdc25-22 cells with a microtubule-destabilizing drug during an arrest is known to cause a premitotic arrest with low activity of the
mitosis-promoting factor (MPF), it was assumed that an impaired integrity of microtubules and/or lack of tubulin dimers in
the nucleus were responsible for the delayed mitosis initiation in cdc25-22 alp1-1315 cells and in cdc25-22 cells treated with a microtubule-destabilizing drug. The progression through mitosis after a cdc25-22 arrest was extremely slow in cdc25-22 alp1-1315 cells, which was attributed to the de novo formation of tubulin dimers. 相似文献
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The life cycle and developmental stages of Aylax hypecoi (Trotter, 1913, Hymenoptera: Cynipidae: Aylacini) were studied in detail. Aylax hypecoi is known to induce galls in fruits of two Hypecoum species — H. imberbe and H. geslini (Papaveraceae) and the larva develops in host plant fruits. The morphology and development of egg, larva and pupa were investigated, which
has previously not been done. The shape and size of terminal-instar larvae and associated galls are sex-specific. Overwintering
stage, adult emergence and flying periods, and egg productivity were studied also. 相似文献
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The influence of sugars and growth regulators on shoot and root growth of Dactylorhiza species was studied under in vitro conditions. The seedling development was stimulated with the application of glucose and sucrose at concentration of 10 g
dm−3 each. The improvement of shoot growth rate and shoot length was enhanced by cytokinins N
6-(2-isopentenyl)adenine or N
6-benzyladenine and their combination with auxin indolebutyric acid (IBA). The root growth rate and root length of seedlings
increased in the presence of IBA and α-naphthaleneacetic acid. Individual Dactylorhiza species showed statistically significant differences in shoot and root development depending on sugar and growth regulator
combinations. 相似文献