共查询到20条相似文献,搜索用时 15 毫秒
1.
Background
The mechanism underlying autoimmune diabetes has been difficult to define. There is a strong genetic contribution and numerous studies associate the major histocompatibility complex, especially the class II region, with predisposition or resistance. However, how these molecules are implicated remains obscure. 相似文献2.
Ulrika Blank Marianne L Seto Derek C Adams Don M Wojchowski Michele J Karolak Leif Oxburgh 《BMC developmental biology》2008,8(1):86
Background
Bone morphogenetic proteins (BMPs) regulate essential processes during organogenesis, and a functional understanding of these secreted proteins depends on identification of their target cells. In this study, we generate a transgenic reporter for organogenesis studies that we use to define BMP pathway activation in the developing kidney. 相似文献3.
Mingjun Zhang Scott C Lenaghan Lijin Xia Lixin Dong Wei He William R Henson Xudong Fan 《Journal of nanobiotechnology》2010,8(1):20
Background
The search for naturally occurring nanocomposites with diverse properties for tissue engineering has been a major interest for biomaterial research. In this study, we investigated a nanofiber and nanoparticle based nanocomposite secreted from an insect-capturing plant, the Sundew, for cell attachment. The adhesive nanocomposite has demonstrated high biocompatibility and is ready to be used with minimal preparation. 相似文献4.
Jorge Vieira Pedro G Ferreira Bruno Aguiar Nuno A Fonseca Cristina P Vieira 《BMC evolutionary biology》2010,10(1):200
Background
Within Rosaceae, the RNase based gametophytic self-incompatibility (GSI) system has been studied at the molecular level in Maloideae and Prunus species that have been diverging for, at least, 32 million years. In order to understand RNase based GSI evolution within this family, comparative studies must be performed, using similar methodologies. 相似文献5.
Pedro Ângelo Silva Reiaz Ul-Rehman Cláudia Rato Gian-Pietro Di Sansebastiano Rui Malhó 《BMC plant biology》2010,10(1):179
Background
The continuous polarized vesicle secretion in pollen tubes is essential for tip growth but the location of endo- and exocytic sub-domains remains however controversial. In this report we aimed to show that Arabidopsis thaliana syntaxins are involved in this process and contribute to spatially define exocytosis and membrane recycling. 相似文献6.
7.
Derrick K Rollins Dongmei Zhai Alrica L Joe Jack W Guidarelli Abhishek Murarka Ramon Gonzalez 《BMC bioinformatics》2006,7(1):377
Background:
The highly dimensional data produced by functional genomic (FG) studies makes it difficult to visualize relationships between gene products and experimental conditions (i.e., assays). Although dimensionality reduction methods such as principal component analysis (PCA) have been very useful, their application to identify assay-specific signatures has been limited by the lack of appropriate methodologies. This article proposes a new and powerful PCA-based method for the identification of assay-specific gene signatures in FG studies. 相似文献8.
9.
Background
Acinetobacter baumannii is an emerging nosocomial pathogen worldwide with increasing prevalence of multi-drug and pan-drug resistance. A. baumannii exists widely in natural environment, especially in health care settings, and has been shown difficult to be eradicated. Bacteriophages are often considered alternative agent for controlling bacterial infection and contamination. In this study, we described the isolation and characterization of one virulent bacteriophage AB1 capable of specifically infecting A. baumannii. 相似文献10.
Monika Witusik Sylwester Piaskowski Krystyna Hulas-Bigoszewska Magdalena Zakrzewska Sylwia M Gresner S Ausim Azizi Barbara Krynska Pawel P Liberski Piotr Rieske 《BMC biotechnology》2008,8(1):56
Background
Although extensive research has been performed to control differentiation of neural stem cells – still, the response of those cells to diverse cell culture conditions often appears to be random and difficult to predict. To this end, we strived to obtain stabilized protocol of NHA cells differentiation – allowing for an increase in percentage yield of neuronal cells. 相似文献11.
Márcia R Soares Agda P Facincani Rafael M Ferreira Leandro M Moreira Julio CF de Oliveira Jesus A Ferro Maria IT Ferro Rogério Meneghini Fábio C Gozzo 《Proteome science》2010,8(1):55
Background
Citrus canker is a disease caused by Xantomonas citri subsp.citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. In this work, the first proteome of Xac was analyzed using two methodologies, two-dimensional liquid chromatography (2D LC) and tandem mass spectrometry (MS/MS). 相似文献12.
Jacob Glenting Lars K Poulsen Kentaro Kato Søren M Madsen Hanne Frøkiær Camilla Wendt Helle W Sørensen 《Microbial cell factories》2007,6(1):28
Background
Natural allergen sources can supply large quantities of authentic allergen mixtures for use as immunotherapeutics. However, such extracts are complex, difficult to define, vary from batch to batch, which may lead to unpredictable efficacy and/or unacceptable levels of side effects. The use of recombinant expression systems for allergen production can alleviate some of these issues. Several allergens have been tested in high-level expression systems and in most cases show immunereactivity comparable to their natural counterparts. The gram positive lactic acid bacterium Lactococcus lactis is an attractive microorganism for use in the production of protein therapeutics. L. lactis is considered food grade, free of endotoxins, and is able to secrete the heterologous product together with few other native proteins. Hypersensitivity to peanut represents a serious allergic problem. Some of the major allergens in peanut have been described. However, for therapeutic usage more information about the individual allergenic components is needed. In this paper we report recombinant production of the Ara h 2 peanut allergen using L. lactis. 相似文献13.
Background
Cyclic nucleotides are ubiquitous intracellular messengers. Until recently, the roles of cyclic nucleotides in plant cells have proven difficult to uncover. With an understanding of the protein domains which can bind cyclic nucleotides (CNB and GAF domains) we scanned the completed genomes of the higher plants Arabidopsis thaliana (mustard weed) and Oryza sativa (rice) for the effectors of these signalling molecules. 相似文献14.
Background
H-NS regulates the acid stress resistance. The present study aimed to characterize the H-NS-dependent cascade governing the acid stress resistance pathways and to define the interplay between the different regulators. 相似文献15.
Cynthia J Bishop David M Aanensen Gregory E Jordan Mogens Kilian William P Hanage Brian G Spratt 《BMC biology》2009,7(1):3-20
Background
Methods for assigning strains to bacterial species are cumbersome and no longer fit for purpose. The concatenated sequences of multiple house-keeping genes have been shown to be able to define and circumscribe bacterial species as sequence clusters. The advantage of this approach (multilocus sequence analysis; MLSA) is that, for any group of related species, a strain database can be produced and combined with software that allows query strains to be assigned to species via the internet. As an exemplar of this approach, we have studied a group of species, the viridans streptococci, which are very difficult to assign to species using standard taxonomic procedures, and have developed a website that allows species assignment via the internet. 相似文献16.
Yin Tat Chan Reginald B. H. Tan Hsien Hui Khoo 《The International Journal of Life Cycle Assessment》2012,17(1):89-95
Purpose
Life cycle assessment (LCA) practitioners in Singapore currently rely on foreign life cycle impact assessment (LCIA) methodologies when conducting studies, despite the fact that foreign methodologies may not be relevant, adaptable and sensitive to Singapore's circumstances. As a result, work has been undertaken to develop the Singapore IMPact ASSessment (SIMPASS) methodology by adapting and modifying existing LCIA methodologies to suit the Singaporean context. It is envisioned that the use of SIMPASS will improve the accuracy of LCA studies conducted for industries operating in Singapore. 相似文献17.
Li-ning Shi Fang-qiu Li Mei Huang Jing-fen Lu Xiao-xiang Kong Shi-qin Wang Hai-feng Shao 《BMC microbiology》2012,12(1):11
Background
There has been a rising incidence of invasive aspergillosis (IA) in critically ill patients, even in the absence of an apparent predisposing immunodeficiency. The diagnosis of IA is difficult because clinical signs are not sensitive and specific, and serum galactomannan has relatively low sensitivity in this group of patients. Therefore, more prompt and accurate disease markers for early diagnosis are needed. To establish disease markers demands a thorough knowledge of fungal antigens which may be detected in the serum or other body fluids of patients. Herein we report novel immunodominant antigens identified from extracellular proteins of Aspergillus fumigatus. 相似文献18.
Josselin Montarry Frédéric M Hamelin Isabelle Glais Roselyne Corbière Didier Andrivon 《BMC evolutionary biology》2010,10(1):283
Background
In gene-for-gene models of plant-pathogen interactions, the existence of fitness costs associated with unnecessary virulence factors still represents an issue, both in evolutionary biology and agricultural sciences. Measuring such costs experimentally has proven difficult, especially in pathogens not readily amenable to genetic transformation, since the creation of isogenic lines differing only by the presence or absence of avirulence genes cannot be achieved in many organisms. Here, we circumvented this difficulty by comparing fitness traits in groups of Phytophthora infestans isolates sharing the same multilocus fingerprint, but differing by their virulence/avirulence spectrum. 相似文献19.
Michael Käser Simona Rondini Martin Naegeli Tim Stinear Francoise Portaels Ulrich Certa Gerd Pluschke 《BMC evolutionary biology》2007,7(1):177
Background
Comparative genomics has greatly improved our understanding of the evolution of pathogenic mycobacteria such as Mycobacterium tuberculosis. Here we have used data from a genome microarray analysis to explore insertion-deletion (InDel) polymorphism among a diverse strain collection of Mycobacterium ulcerans, the causative agent of the devastating skin disease, Buruli ulcer. Detailed analysis of large sequence polymorphisms in twelve regions of difference (RDs), comprising irreversible genetic markers, enabled us to refine the phylogenetic succession within M. ulcerans, to define features of a hypothetical M. ulcerans most recent common ancestor and to confirm its origin from Mycobacterium marinum. 相似文献20.
Ana Laín Natalia Elguezabal Sonia Brena Juan Carlos García-Ruiz Amalia del Palacio María D Moragues José Pontón 《BMC microbiology》2007,7(1):35