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Escargot maintains stemness and suppresses differentiation in Drosophila intestinal stem cells
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Jerome Korzelius Svenja K Naumann Mariano A Loza‐Coll Jessica SK Chan Devanjali Dutta Jessica Oberheim Christine Gläßer Tony D Southall Andrea H Brand D Leanne Jones Bruce A Edgar 《The EMBO journal》2014,33(24):2967-2982
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DPFF‐1 transcription factor deficiency causes the aberrant activation of MPK‐1 and meiotic defects in the Caenorhabditis elegans germline
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Emmanuel Villanueva‐Chimal Laura S. Salinas Laura P. Fernández‐Cardenas Gabriela Huelgas‐Morales Alejandro Cabrera‐Wrooman Rosa E. Navarro 《Genesis (New York, N.Y. : 2000)》2017,55(11)
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Azam Soleimani Nehleh Zarei Fard Tahereh Talaei‐Khozani Soghra Bahmanpour 《Cell biology international》2020,44(9):1850-1859
Three‐dimensional (3D) culture provides a biomimicry of the naive microenvironment that can support cell proliferation, differentiation, and regeneration. Some growth factors, such as epidermal growth factor (EGF), facilitate normal meiosis during oocyte maturation in vivo. In this study, a scaffold‐based 3D coculture system using purified alginate was applied to induce oocyte differentiation from mouse embryonic stem cells (mESCs). mESCs were induced to differentiate into oocyte‐like cells using embryoid body protocol in the two‐dimensional or 3D microenvironment in vitro. To increase the efficiency of the oocyte‐like cell differentiation from mESCs, we employed a coculture system using ovarian granulosa cells in the presence or absence of epidermal growth factor (+EGF or ?EGF) for 14 days and then the cells were assessed for germ cell differentiation, meiotic progression, and oocyte maturation markers. The cultures exposed to EGF in the alginate‐based 3D microenvironment showed the highest level of premeiotic (Oct4 and Mvh), meiotic (Scp1, Scp3, Stra8, and Rec8), and oocyte maturation (Gdf9, Cx37, and Zp2) marker genes (p < .05) in comparison to other groups. According to the gene‐expression patterns, we can conclude that alginate‐based 3D coculture system provided a highly efficient protocol for oocyte‐like cell differentiation from mESCs. The data showed that this culture system along with EGF improved the rate of in vitro oocyte‐like cell differentiation. 相似文献
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