Serological evidence of inter-epidemic infection of feral pigs in New South Wales with Murray Valley encephalitis virus.

The sera of 617 feral pigs, collected from three widely separated areas of northern and central New South Wales, were examined for antibody to Murray Valley encephalitis (MVE) virus and to Ross River virus. Haemagglutination-inhibition (HI) antibody was detected to MVE in 58% of sera and to Ross River virus in 15% of sera. Neutralization tests suggested that the MVE HI antibody resulted from infection with MVE virus in the summers of 1971-1972 and 1972-1973 when the virus was not known to be active in New South Wales. These same tests suggested that more than one flavivirus infected the feral pigs in the summer of 1973-1974 and that Kunjin virus was active in the summer of 1975-1976.     

Further studies on the E blood group system in pigs

The blood group system E in pigs is similar to the B system in cattle, one of the most complicated of the hitherto known blood group systems.
Studies of Andresen (1957), Saison (1958), Andresen & Wroblewski (1959), Andresen et al. (1959), Andresen (1962, 1965), Rasmusen (1965), Hojný et al. (1966), Dinklage et al. (1966), Dinklage & Major (1968), Hradecký & Hojný (1972), Hojný & Hradeck (1972, 1973) lead to the determination of the various E alleles.
This report presents information of a new blood group factor E_p in pigs.     

Besprechungen / Reviews

Book Reviewed in this article: Scourse , N. J. S., und R. A. Hinde (1973): Habituation to auditory stimuli in Mice (Wie Mäuse sich an akustische Reize gewöhnen). Kramer , D. L. (1973): Parental Behaviour in the Blue Gourami Trichogaster trichopterus (Pisces, Belontiidae) and its induction during exposure to varying numbers of conspecific eggs (Das Brutpflegeverhalten des Getüpfelten Guramis und seine Auslösung durch verschieden große Mengen arteigener Eier). Shallenberger , R. J., und W. D. Madden (1973): Luring behaviour in the Scorpion Fish Iracundus signifer (Wie I. s. Beutefische ködert). Chamove , A. S. (1973): Rearing infant Rhesus together (Das Verhalten miteinander aufgezogener Rhesusaffenkinder). Stamps , J. A., und G. W. Barlow (1973): Variation and stereotypy in the displays of Anolis aeneus (Sauria: Iguanidae) (Starrheit und Veränderlichkeit im Ausdrucksverhalten von A. ae.). Moodie , G. E. E., J. D. McPhail , und D. W. Hagen (1973): Experimental demonstration of selective predation on Gasterosteus aculeatus (Stichlinge sind als Beutefische auch im Versuch verschieden stark gefährdet). Maurus , M., und H. Pruscha (1973): Classification of social signals in Squirrel Monkeys by means of cluster analysis (Klassifizierung von Sozialverhaltensweisen des Totenkopfäffchens durch Gruppenanalyse). Wiley , R. H. (1973): The strut display of male Sage Grouse: a “fixed” action pattern (Das Stolzierverhalten männlicher Beifußhühner als “fixiertes” Verhaltensmuster). Sachs , L. (1972): Statistische Methoden. Ein Soforthelfer für Praktiker in Naturwissenschaft, Medizin, Technik, Wirtschaft, Psychologie und Soziologie. Bauer , H. Ch., R. Hofer , W. Knapp und H. Moser (1974): Zoologische Experimente. Kästle , W. (1974): Echsen im Terrarium. 2. Aufl. Reihe: Das Vivarium. Franckh'sche Verlagshandlung Stuttgart. Rensch , Bernhard, und Gerti Dücker (1973): Discrimination of patterns indicating four and five degrees of reward by birds (Vögel lernen vier bis fünf Bilder als Wertmarken für ebenso viele verschiedene Belohnungsstufen zu unterscheiden). Krall , Peter (1973): Der gesunde und der kranke Hund. 9. neubearbeitete Auflage von Georg Müller/Richard Reinhard: Der kranke Hund. Lawick , H. v. (1974): Solo; ein Hundeschicksal in der Serengeti. Verlag Ullstein GmbH, Frankfurt—Berlin—Wien.     

Seasonal and year-to-year differences in food selection by beavers

Summary Selection of trees for food by a colony of beavers in central Massachusetts was studied from September 1972 through April 1974. The beavers exhibited both seasonal and year-to-year differences in preference for certain genera. Pine was selected against during fall but not spring, and there was a switch in preference from birch during fall 1972 to oak and witch hazel in fall 1973. These differences may partly reflect greater seasonal stability in concentrations of stored nutrients in coniferous tree bark than in deciduous tree bark, and greater year-to-year stability in bark concentrations of stored nutrients in non-mast-seeding species than in mast-seeding species (1972 was a mast year for oaks in central Massachusetts).     

Utilization of vitamin E

Natural (RRR) or synthetic (all-rac) forms of alpha-tocopherol are available (usually as acetate esters) for use as vitamin E supplements. In animal tests, the natural stereoisomer, RRR-alpha-tocopheryl acetate, is 1.36 times more biologically potent than all-rac-alpha-tocopheryl acetate, an equimolar mixture of eight stereoisomers [8,15,40-43]. The higher biologic activity of natural compared with synthetic vitamin E does not result from differences in antioxidant activity [2,3], but could hypothetically be explained by differences in (1) absorption, (2) plasma transport, (3) delivery to tissues, or (4) metabolism. These possibilities will be considered in this review.     

Ciampi,E. (1975): Die Welt der Delphine (Übersetzung von: Those other people the porpoises)

Book Reviewed in this article: ed. Kornhuber, H. H. (1974): Handbook of Sensory Physiology Vol. 6: Vestibular System (Das Vestibularsystem). Part 1. Basic Mechanisms. 676 S., 251 Abb., VIII, Preis gebunden 239,— DM bzw. Jung, R. (1973): Visual Perception and Neurophysiology (Gesichtssinn und Neurophysiologie) in: Handbook of Sensory Physiology Vol. Forssmann, W. G., und C. Heym (1974): Grundriß der Neuroanatomie. Longo, V. G. (1972): Neuropharmacology and Behavior (Neuropharmakologie und Verhalten). W. H. Freeman and Comp. Curry-Lindahl, K. (1975): Groddjur och kräldjur i färg (Lurche und Kriechtiere in Farben). Verlag Almqvist und Wiksel, Stockholm. Ders. (1975): Däggdjur i färg (Säugetiere in Farben). Griffin, D. R. (1974): Bird Migration. The Biology and Physics of Orientation (Vogelzug. Biologische und physikalische Grundlagen der Orientierung). Griffin, D. R. (1974): Listening in the Dark. The acoustic orientation of Bats and Men (Horchen im Dunkeln). Douglas-Hamilton, I. und O. (1976): Unter Elefanten. R. Piper & Co. Wieser, W. (1976): Konrad Lorenz und seine Kritiker. ed. Autrum, H., und U. Wolf (1973): Humanbiologie. Ergebnisse und Aufgaben. Heidelberger Taschenbücher Bd. 121. Steitz, E. (1974): Die Evolution des Menschen. Arbeitsbücher Biologie, Taschentext 16. ed. Fox, R. (1975): Biosocial Anthropology (Biosoziale Anthropologie).     

Martian channels and the search for extraterrestrial life

Summary The origin of the channels on Mars has been a subject of intense interest since they were first recognized on early Mariner 9 images (Driscoll, 1972; Masursky, 1973). Their presence on the planet, and their striking resemblance to terrestrial flood channels related to glacial outbursts or to dendritic river systems has suggested to most investigators (Baker, 1974, 1977; Nummedal, 1978; Carr, 1979; Masursky et al., 1977) that they were formed by running water. Because life as we know it is dependent on water, the discovery by the Mariner cameras, of watercut channels and volcanoes as a source for water, and water ice in the residual north polar cap by Viking, has reaffirmed the choice of Mars as the best target for the search for extraterrestrial life.     

Molecular mapping of murine I region recombinants: crossing over in the E beta gene

The parental origin of genomic DNA from two independently derived murine I-region recombinants, B10.ASR7 [as3] and B10.BASR1 [as4], was determined by Southern blot hybridization by using DNA probes corresponding to A beta, A alpha, 5'-E beta, 3'-E beta, and A alpha genes. New E beta gene probes were specifically constructed to make analysis of the E beta gene region definitive. Although the immune response phenotypes of the recombinants had suggested an I-A subregion cross-over, a number of restriction fragment length polymorphisms distinguishing the k and the s haplotypes showed that both recombinations mapped within a 7-kb segment of the E beta gene. The validity of these results was tested by analysis of two other H-2k/s recombinants. One of them, B10.S(8R) [as1], mapped within the same 7-kb region of the E beta gene, whereas the other, B10.BASR2 [as5], mapped outside the I-region as expected. Including those studied here, there are a dozen I region recombinants whose cross-over positions have been determined at a molecular genetic level, and all of the cross-overs occurred within the E beta gene.     

Hyperlipidemia and reproductive failure in captive-reared alligators: vitamin E, vitamin A, plasma lipids, fatty acids, and steroid hormones

Blood samples were collected from 26 captive-reared alligators (25 females; one male) and 12 (seven females and five males) wild "nuisance" alligators collected by wildlife personnel in south Louisiana in May 1995. The captive alligators, hatched from artificially incubated eggs in 1972-1973, had received vitamin E supplements during the 3 weeks before the blood sample was collected. Each sample was analyzed for vitamin E (alpha-tocopherol), vitamin A (retinol), total lipid, triacylglycerol, phospholipid, cholesterol, cholesteryl ester, free fatty acids, steroid hormones and a standard clinical blood panel. The fatty acid composition of the plasma lipid fraction was also analyzed. Results indicated that 18 of the captive females and three of the seven wild females were undergoing vitellogenesis, i.e. had elevated plasma estradiol and elevated plasma calcium. Vitellogenic females had higher vitamin E than non-vitellogenic females (77.4 microg/ml vs. 28.6 microg/ml in captive females; 24.0 microg/ml vs. 21 microg/ml in wild females). Plasma retinol was similar in all groups, ranging from 0.5 to 1.4 microg/ml and close to values reported in birds. All lipid fractions, with the exception of cholesteryl ester, were higher in captive alligators than in wild alligators. There were also significant differences in the fatty acid composition of wild and captive alligators. Plasma eicosapentaenoic and docasahexaenoic acid were higher in wild than in captive alligators, whereas linoleic was higher in captive than in wild.     

Incidence of resistance to ampicillin,chloramphenicol, kanamycin and tetracycline amongSalmonella species isolated in The Netherlands in 1972, 1973 and 1974

The resistance ofSalmonellae to drugs has been studied in the Netherlands since 1958. In 1972, 1973, and 1974 respectively, 14241, 13086, and 22927 strains were tested for resistance to ampicillin, chloramphenicol, kanamyci and tetracycline. From 1973 all strains were also tested for resistance to trimethoprim. In the period covered, the yearly incidence of resistance to at least one of the above drugs ranged from 39.2% to 45.6% of all strains obtained from various sources (humans, animals, animal products, sewage, etc.). A new finding in the period 1972 to 1974 was that many multiply resistant strains emerged inS. typhimurium and inS. dublin isolated from calves and cattle. In 1974, 64.4% of all strains ofS. typhimurium from these animals appeared to be resistant to ampicillin, tetracycline, chloramphenicol and kanamycin, and 25.5% of those ofS. dublin were found to be resistant to chloramphenicol and tetracycline. Of all strains ofSalmonellae examined in 1973 and 1974 respectively, 0.15 and 0.22% were resistant to trimethoprim, the main component of the twin-drug cotrimoxazol. Of the 142 strains ofS. typhi isolated in 1972 to 1974 two were resistant to tetracycline only, and one was resistant to all four antibiotics. The others had a normal susceptibility pattern.     

Model for the chemotactic response of a bacterial population.

We present a mathematical model for the motion of a bacterial population in prescribed attractant or repellent gradients. The model is suggested by the observations of Mesibov et al. (1973, J. Gen. Physiol. 62:203) and Brown and Berg (1974, Proc. Natl. Acad. Sci. U.S.A. 71:1388) who found that the sensitivity of the chemotactic response depends on the concentration of attractant. Predictions of the theory are in general agreement with the experiments of Dahlquist et al. (1972, Nat. New Biol. 236:120) and of Mesibov et al. on populations of motile bacteria in fixed attractant gradients. Additional tests of the model are proposed.     

Testing for conditional multiple marginal independence

Survey respondents are often prompted to pick any number of responses from a set of possible responses. Categorical variables that summarize this kind of data are called pick any/c variables. Counts from surveys that contain a pick any/c variable along with a group variable (r levels) and stratification variable (q levels) can be marginally summarized into an r x c x q contingency table. A question that may naturally arise from this setup is to determine if the group and pick any/c variable are marginally independent given the stratification variable. A test for conditional multiple marginal independence (CMMI) can be used to answer this question. Since subjects may pick any number out of c possible responses, the Cochran (1954, Biometrics 10, 417-451) and Mantel and Haenszel (1959, Journal of the National Cancer Institute 22, 719-748) tests cannot be used directly because they assume that units in the contingency table are independent of each other. Therefore, new testing methods are developed. Cochran's test statistic is extended to r x 2 x q tables, and a modified version of this statistic is proposed to test CMMI. Its sampling distribution can be approximated through bootstrapping. Other CMMI testing methods discussed are bootstrap p-value combination methods and Bonferroni adjustments. Simulation findings suggest that the proposed bootstrap procedures and the Bonferroni adjustments consistently hold the correct size and provide power against various alternatives.     

Transplantation phenomena in hydra: Cooperation of position-dependent and structure-dependent factors determines the transplantation result

Hydra transplantation phenomena were examined using lateral grafting (Browne, 1909; Wolpert et al., 1974) and axial grafting (Shostak, 1972, Shostak, 1973, Shostak, 1974; Hicklin et al., 1973) procedures. When a small piece of tissue excised from one hydra is transplanted to another by the former procedure, the transplanted tissue forms a small protrusion on the body column of the host animal, whereas such a protrusion is not produced by the latter procedure. It was found that transplantation experiments carried out by the two procedures gave significantly different results. The frequency of head formation by the transplanted tissue on the host was significantly higher by the lateral than by the axial grafting procedure. This suggests that the small protrusion introduced onto the smooth cylindrical structure of the host body column by the former, but not by the latter, procedure plays an important role in determining the fate of the transplant. It is suggested that the cooperation of two factors determines the transplantation result. One is the positional factor described and discussed by previous workers (e.g., Wolpert et al., 1974; Sugiyama, 1982; MacWilliams, 1983, MacWilliams, 1983; Takano and Sugiyama, 1983) and the other is the structural factor revealed in this study. The underlying mechanisms responsible for the latter factor are discussed.     

Environmental Impacts of Products:Policy Relevant Information and Data Challenges

The research and analysis presented in this special issue shows that the same limited number of consumption categories are consistently revealed to be responsible for the largest share of environmental impact: mobility (automobile and air transport), food (meat, poultry, fish, and dairy followed by plant‐based food), and residential energy use in the house (heating, cooling, electrical appliances, and lighting). It appears that differences in impact per euro between the product groupings are relatively limited, so it is essential to reduce the life‐cycle impacts of products as such, rather than to shift expenditures to less impact‐intensive product groupings. Furthermore, the effectiveness of expenditure on material products to improve quality of life leaves much room for improvement. Environmentally extended input‐output (EEIO) tables probably form, in this field, the most appropriate information support tool for priority setting, prospective assessment of options, scenario analysis, and monitoring. A clear benefit would result from integrating the input–output (IO) tables in the report to Eurostat of the 25 individual countries that make up the European Union (EU), with other officially available information on emissions and resources use, into a 60‐sector EEIO table for the EU. This, obviously, would be the first step toward more detailed tables. Three strategies are suggested to realize the additional, desirable detail of 150 sectors or more, each achievable at an increasing time horizon and with increasing effort: (1) developing further the current CEDA EU25 table; (2) building a truly European detailed input–output table accepting the restrictions of existing data gathering procedures; and (3) as (2), but developing new, dedicated data gathering and classification procedures. In all cases, a key issue is harmonizing classification systems for industry sectors, consumer expenditure categories, and product classifications (as in import/export statistics) in such a way that data sets may adequately be linked to input–output tables.     

The Indirect Fluorescent Antibody Test (IFAT) for the Detection of Nosema Cuniculi Antibodies in the Blue Fox (Alopex Lagopus)

During recent years nosematosis has been a major problem in the breeding of blue fox in the Scandinavian countries, causing heavy losses among growing pups (Nordstoga 1972, Nordstoga et al. 1974). The lack of reliable methods for diagnosing the infection in live foxes has so far made epizootiologic studies of the disease very difficult. However, reports on the IFAT in rabbits with nosematosis (Cox et al. 1972, Chalupsky et al. 1971, 1973, 1974), encouraged the search for a method of detecting Nosema antibodies in fox sera.     

Hemoparasites of raccoons (Procyon lotor) in Florida

Four hemoparasite species (Babesia lotori, Trypanosoma cruzi, Dirofilaria tenuis and Mansonella llewellyni) were found in raccoons (Procyon lotor) collected from 1972 to 1974 in Duval (n = 14) and Collier (n = 170) counties, Florida (USA). Trypanosoma cruzi was found in thin blood smears from one raccoon at each locality. The prevalence of B. lotori was 79% and 80% in samples taken in December 1973 in Collier and Duval counties, respectively. No patent infections by B. lotori were detected in raccoons collected in Collier County in December 1972, but 42% of the raccoons examined in September 1973 were infected. In Collier County there were no significant differences in the prevalence of B. lotori by host sex or age. In Duval County, overall D. tenuis prevalence was 7%, whereas that of M. llewellyni was 14%; the latter species was not found in Collier County. Adult raccoons had a significantly greater prevalence of D. tenuis (32%) than did subadults and juveniles (7%), and male raccoons showed a significantly greater prevalence (51%) than did females (8%).     

Urinary leukotriene E4 excretion is increased in type 1 diabetic patients: a quantification by liquid chromatography-tandem mass spectrometry

OBJECTIVE: Diabetes mellitus is associated with inflammatory state and increased cardiovascular mortality. Leukotrienes are arachidonic acid metabolites derived from the 5-lipoxygenase pathway that possess vasoactive, chemotactic and proinflammatory properties. The aim of this study was to evaluate (1) the urinary excretion of leukotriene E4 (LTE4) in type 1 diabetic subjects and healthy volunteers and (2) the influence of glycemic control attested by HbA(1C) on LTE4 excretion. METHODS AND RESULTS: Urinary excretion of LTE(4), measured by liquid chromatography-tandem mass spectrometry, was significantly (P=0.033) increased in diabetic patients (median [10th-90th percentiles]: 42.1 pg/mg creatinine [16.7-71.4], n=34), compared to healthy subjects (25.5 pg/mg creatinine [13.9-54.1], n=28). Subgroup analysis indicated a trend towards increased LTE4 excretion in patients with poor glycemic control [(HbA(1C)> or =9% or plasma glucose >18 mmol/L): 43.3 pg/mg creatinine [21.6-70.5], n=14], whereas no difference was observed between patients with good metabolic control [(HbA(1C)< or =7.5%): 36.4 pg/mg creatinine [15.8-83.4], n=20] and healthy subjects. CONCLUSIONS: This study suggested that increased LTE4 excretion in type 1 diabetic state might reflect systemic activation of the 5-lipoxygenase pathway. It could be a determinant of underlying inflammatory state and vascular disease.     

Activity analysis of neural networks

In a series of articles (Leung et al., 1973, 1974; Ogztöreli, 1972, 1975, 1978, 1979; Stein et al., 1974) we have investigated some of the physiologically significant properties of a general neural model. In these papers the nature of the oscillations occuring in the model has been briefly analyzed by omitting the effects of the discrete time-lags in the interaction of neurons, although these time-lags were incoporated in the general model. In the present work we investigate the effects of the time-lags on the oscillations which are intrinsic to the neural model, depending on the structural parameters such as external inputs, interaction coefficients, self-inhibition, self-excitation and selfadaptation coefficients. The numerical solution of the neural model, the computation of the steady-state solutions and the natural modes of the oscillations around the steady-state solutions are described.This work was partly supported by the Natural Sciences and Engineering Research Council of Canada under Grant NRC-A-4345 through the University of Alberta     

Meta‐analysis of rare events under the assumption of a homogeneous treatment effect

We studied the performance of several meta‐analysis methods in rare event settings, when the treatment effect is assumed to be homogeneous and baseline prevalences are either homogeneous or heterogeneous. We conducted extensive simulations that included the three most common effect sizes with count data: the odds ratio, the relative risk, and the risk difference. We investigated several important scenarios by varying the level of rareness, the value of the trials’ arms unbalance, and the size of the treatment effect. We found that the Mantel–Haenszel method and the Binomial regression model provided the best results across all the scenarios investigated. The Peto method performed satisfactorily only when the true effect size was not too large and the degree of unbalance moderate. Inverse variance was the least reliable method. The use of a continuity correction factor slightly improved the performance of the inverse variance method but deteriorated that of the Peto and Mantel–Haenszel methods. A method based on median unbiased estimators of the probabilities provided similar results to those obtained when using the inverse variance method with a continuity correction. Therefore, when the treatment effect can be assumed to be homogeneous and for either homogeneous or heterogeneous baseline prevalences, we highly recommend using the Mantel‐Haenszel method without continuity correction (for all the effect sizes) or the Binomial regression model (for the odds ratio only) to meta‐analyze the data.