米仓山自然保护区水青冈属(Fagus)资源调查报告

使用常规野外种群调查方法调查了米仓山自然保护区中水青冈属(Fagus)林木资源贮量。结果表明保护区有水青冈属分布的林分面积达7 064.8 hm2,占保护区有林地面积40%多;水青冈属活立木总蓄积814 600 m3,是国内目前水青冈属植物保存面积最大的地区。保护区中现有水青冈(F.longipetiolata),亮叶水青冈(F.lucida),台湾水青冈(F.hayatae),米心水青冈(F.engleriana)4个种,而在这4种水青冈属植物中,又以台湾水青冈的发现最为重要,有台湾水青冈分布的林分面积达5 923.3 hm2,以台湾水青冈为优势的林分面积2 511 hm2,台湾水青冈活立木蓄积317 000 m3。     

五个中国荞麦(Fagopyrum)种的核型分析

用去壁低渗法对甜荞 ( Fagopyrum esculentum)、苦荞 ( F.tataricum)、左贡野荞 ( F.zuogongense Q.F.Chen) ,大野荞 ( F.megaspartanium Q.F.Chen)及毛野荞 ( F.pilus Q.F.Chen)等大粒组荞麦种的根尖和茎尖有丝分裂染色体进行了观察 ,并对其茎尖有丝分裂染色体的核型进行了比较分析。结果表明 :这 5种荞麦在核型上类似 ,都有 2对随体染色体 ,而且都为对称核型。但它们彼此有一定的差异。甜荞、苦荞、大野荞、毛野荞及左贡野荞的核型公式分别为 1 2 m+4m( SAT)、1 2 m+4sm( SAT)、8m+4sm+4m( SAT)、1 2 m+2 m( SAT) +2 sm( SAT)及 2 4 m+4sm+4m( SAT)。     

Suppression of ρ 0 lethality by mitochondrial ATP synthase F1 mutations in Kluyveromyces lactis occurs in the absence of F0

Specific mgi mutations in the α, β or γ subunits of the mitochondrial F₁-ATPase have previously been found to suppress ρ⁰ lethality in the petite-negative yeast Kluyveromyces lactis. To determine whether the suppressive activity of the altered F₁ is dependent on the F₀ sector of ATP synthase, we isolated and disrupted the genes KlATP4, 5 and 7, the three nuclear genes encoding subunits b, OSCP and d. Strains disrupted for any one, or all three of these genes are respiration deficient and have reduced viability. However a strain devoid of the three nuclear genes is still unable to lose mitochondrial DNA, whereas a mgi mutant with the three genes inactivated remains petite-positive. In the latter case, ρ⁰ mutants can be isolated, upon treatment with ethidium bromide, that lack six major F₀ subunits, namely the nucleus-encoded subunits b, OSCP and d, and the mitochondrially encoded Atp6, 8 and 9p. Production of ρ⁰ mutants indicates that an F₁-complex carrying a mgi mutation can assemble in the absence of F₀ subunits and that suppression of ρ⁰ lethality is an intrinsic property of the altered F₁ particle. Received: 7 April 1998 / Accepted: 10 June 1998     

Characterization of the laser-induced blue, green and red fluorescence signatures of leaves of wheat and soybean grown under different irradiance

The blue, green and red fluorescence emission of green wheat ( Triticum aestivum L. var. Rector) and soybean leaves ( Glycine max L. var. Maple Arrow) as induced by UV light (nitrogen laser: 337 nm) was determined in a phytochamber and in plants grown in the field. The fluorescence emission spectra show a blue maximum near 450 nm, a green shoulder near 530 nm and the two red chlorophyll fluorescence maxima near 690 and 735 nm. The ratio of blue to red fluorescence, F450/F690, exhibited a clear correlation to the irradiance applied during the growth of the plants. In contrast, the chlorophyll fluorescence ratio, F690/F735, and the ratio of blue to green fluorescence, F450/F530, seem not to be or are only slightly influenced by the irradiance applied during plant growth. The blue fluorescence F450 only slightly decreased, whereas the red chlorophyll fluorescence decreased with increasing irradiance applied during growth of the plants. This, in turn, resulted in greatly increased values of the ratio, F450/F690, from 0.5 – 1.5 to 6.4 – 8.0. The decrease in the chlorophyll fluorescence with increasing irradiance seems to be caused by the accumulation of UV light absorbing substances in the epidermal layer which considerably reduces the UV laser light which passes through the epidermis and excites the chlorophyll fluorescence of the chloroplasts in the subepidermal mesophyll cells.     

Distribution and pollution evaluation of fluoride in a soil–water–plant system in Shihezi,Xinjiang, China

Fluoride (F) pollution is a serious environmental problem in some areas of China, but it has yet to be reported in a soil–water–plant system in Shihezi, Xinjiang. This study was undertaken to investigate the distribution and migration rule of F in soil, water, and plants, and to evaluate F pollution of soil. Results showed that the average concentration of total F (T-F) in the topsoil in the northwest, north, and southeast of Shihezi was higher than the national average T-F (478 mg/kg), while it was lower in southwest. The highest T-F contents of the soil profile were detected in the depth of 20 cm. The F content in groundwater in the northwest region was higher than the GB/T 14848–93 (1.0 mg/L), whereas the F contents in other water samples were within the standard. The F contents (1.75?2.81 mg/kg) in plant leaves were higher than the food limits (1.0 mg/kg). The obtained comprehensive pollution index of the soil was 1.86, which means a mild concentration of F in Shihezi. This research has reference value for the study of F pollution and comprehensive control in the northwest oasis with the typical arid and saline conditions.     

A Karyological Study on Fritillaria from Xinjiang

Fritillary is a precious Chinese medicinal herb. Those native to Xinjiang Northwest China, are even more distinguished from other sources for their purity and effectiveness. Fritillaria in Xinjiang comprises 8 native species and one (F. thunbergii Miq.) introduced from Zhejiang, East China. In this paper the authors describe the karyotypes of 6 species native to Xinjiang and F. thunbergii Miq., of which five, i.e.F. olgae Vved., F. walujewii Regel, F. yuminensis X. Z. Duan, F. karelinii (Fisch.) Baker and F. thunbergii Miq. were studied for the first time. Detail observation and measurment of chromosomes in each of them were made. The data obtained may be summarized as follows: scietific name karyotype formula (2n=) F. pallidiflora Schrenk 2m + 2sm + 6st + 14t F. olgae Vved. 4m + 6st + 14t F. walujewii Regel 2m + 2sm + 8st+ 12t F. yuminensis X. Z. Duan 4m + 8st + 12t F. verticillata Willd 4m + 8st + 12t F. karelinii (Fisch.) Baker 4m + 4sm + 4st + 12t F. thunbergii Miq. 2m + 2sm + 4st + 16t The karyotype of the native species are, on the whole, similar to each other except that of F. karelinii (Fisch.) Baker, a species inhabiting desert areas. The number of m-sm chromosomes has increased from 2 to 4 and the number of st-t chromosomes decreased correspondently. So is the karyotype of F. thunbergii Miq. which is noted for its high ratio of long chromosome/short chromosome and the more t-chromosomes. These two peculiar karyotypes coincide amazingly with their specific natural habitats.     

秦岭产珠子参叶的达玛烷型皂甙研究(1)

从陕西省秦岭产珠子参(Panax japonicus C.A.Meyer var.major(Burk.)Wu etFeng)的叶中分离到十个新的达玛烷型四环三萜皂甙,经光谱测定和化学降解,其中四个的化学结构分别为珠子参甙(majoroside)F_1(1)、F_2(2)、F_3(3)和F_4(4)。同时,还分离到已知的人参甙(ginsenoside)Rd(5)、Re(6)、Rg_1(7)、Rg_2(8)和F_2(9)。     

中国大鲵子二代适应能力及生长优势的研究

2002年成功孵化出大鲵子二代1260尾,经过8个月生长,随机抽取172尾全长13～15 cm、体重25～35 g子二代与172尾全长14～16.5 cm、体重30～40 g野生代进行环境适应能力和生长速度的对比试验.结果表明,子二代在耐高温、耐低氧、耐不良水质、耐盐度、耐酸碱性、耐苦味饵料方面比野生代有明显优势,说明子二代适应能力更强,易于饲养.从生长优势方面,表现出大鲵子二代生长速度快,生长指标为4.4928,而野生代生长指标为3.4714.     

杂交鳢(斑鳢 ♀ ×乌鳢 ♂ )及其自交后代细胞核型初步分析

对杂交鳢(斑鳢♀×乌鳢♂)(Channa maculata ♀×C.argus ♂)及其自交后代的细胞核型进行了初步分析.结果表明,杂交鳢染色体数目为2n=45,核型公式为3m+4sm+6st+32t,染色体臂数(NF)为52;杂交鳢自繁后代群体存在两种染色体核型,一是染色体数目为45,核型公式为3m+4sm+6st+...     

穿鞘菝葜的化学成分

从穿鞘菝葜(Smilax perfoliata Lour.)的95%乙醇提取物的乙酸乙酯萃取部分分离得到6个化合物,其中有一个新化合物smilglaside F(1).经过核磁共振、质谱、红外光谱及化学手段鉴定了它们的结构.5个已知化合物分别是:芦丁(2)、3′-甲醚芦丁(3)、cassiamin A(4)、cassiamin B(5)和1,2,3-trimethoxy-5-hydroxyphenol-1-O-β-D-glucopyranoside(6).其中化合物2、4～6是首次从该属植物分离得到.     

Height, trade, and inequality in the Latin American periphery, 1950-2000.

Which variables determine whether a country chooses an open or protected market? It has been argued that economic downturn leads to a higher propensity for protectionism. We find for seven Latin American countries in the second half of the 20th century that declining GDP motivated the opening wave, especially during the 1980s. Moreover, inequality could play a role, either in favor of "opening", as Stolper-Samuelson models would predict, or in favor of closing, as recent empirical studies found that open periods were associated with higher inequality. Using anthropometric indicators, we find that inequality in general tended to motivate "closing", whereas inequality did not stimulate opening.     

A new species ofFontanesia (Oleaceae) from China and taxonomic revision of the genus

Fontanesia longicarpa K.-J. Kim is newly described from China. This distinctive species is known only from the Zhejiang province and differs from other species in the genus by the size and shape of its fruit. As currently circumscribed, the genus now consists of three taxa;F. longicarpa, F. philliraeoides Labill. var.philliraeoides andF. philliraeoides var.fortunei (Carr.) Koehne. A revisionary study of the genus is provided, including a key and pertinent synonymy.     

湘中锡矿山弗拉期晚期腕足动物一新属

描述产于锡矿山弗拉期晚期腕足动物Ｔｒｉｇｏｎｉｒｈｙｎｃｈｉｉｄａｅ的一个属－Ｈｕｎａｎｏｔｏｅｃｈｉａ,包括２新种：Ｈ．ｔｉｅｎｉ ｓｐ．ｎｏｖ．和Ｈ．ｅｌｏｎｇａｔａ ｓｐ．ｎｏｖ．。该属在弗拉阶顶部绝灭。     

利用重组荧光素酶报告基因载体研究E2F调控组蛋白H2A的转录

转录因子E2F与细胞增殖、凋亡及癌变密切相关,组蛋白H2A是构成核小体的重要成员之一.研究通过特异性的阻断Rb基因的表达发现组蛋白H2A家族成员:HIST1H2AJ表达下调,再进一步研究转录因子E2F对HIST1H2AJ的转录调控.通过PCR得到HIST1H2hJ的5非翻译区序列,克隆到荧光素酶报告载体pGL3中,然后转染入HEK293,再检测荧光素酶的活性来判断E2F是否调控HIST1H2AJ的转录.研究结果表明:转录因子E2F能够调控组蛋白H2A家族成员之一HIST1H2AJ的转录.     

Contributo preliminare alla sistematica di Chamaecytisus hirsutus e Chamaecytisus supinus

Abstract

Chamaecytisus hirsutus and C. supinus: a preliminary report. – Chamaecytisus hirsutus (L.) Link and C. supinus (L.) Link have been described as twostrictly related species, which differ mainly in the form of inflorescence: C. hirsutus has lateral flowers, C. supinus has terminal flowers. Besides, most Floras provide a set of additional differential characters that should permit to di-C. hirsutus and C. supinus.

The author analyses such differential characters and demonstrates that all of them are inconsistent: therefore the inflorescence seems to be the only difference between C. hirsutus and C. supinus.

The inflorescence itself, however, is not a constant character: indeed, it is known that C. supinus may develop vernal latera flowers besides the normal aestival terminal ones.

The geographic distributions of the two inflorescence types are accurately examined (a report of the distributions is given, a list of herbarium specimens is presented in appendix, a point distribution map of the two types is given in figs. 1 and 2); the only differences are the following ones: plants with flowers in leafy racemes (usually identified as C. hirsutus) seem to be absent from Spain and from Southern Poland, and to be unfrequent in Central and Western France; instead, plants with flowers in heads (usually identified as C. supinus) are very unfrequent in the Southern Balcan Peninsula, and are absent from Southern Greece and the Italian Peninsula.

After a discussion of the biological significance of the capitate and lateral inflorescence, and on the basis of in vivo observations, the author argues that probably the same taxonomic unit is present in the whole area, showing some differences in its flowering behaviour; in the largest part of the areal – including the center of distribution of the species – most individuals flower twice, and therefore have been recorded as two different species; a trend toward the capitate inflorescence is remarkable in the North and in the West; instead, in the South and the East the trend is toward lateral flowers (fig. 3).

Further biometrical and biochemical studies on the species are now in progress; more observations in field in different parts of Europe are necessary in order to get conclusive evidence of the identity of these two so-called «species».     

松蜕盾蚧等四种蜕盾蚧分类地位的研究（同翅目：盾蚧科）

将松蜕盾蚧Florinia pinicola Maskell接种于盆栽马尾松上饲养,得到大量标本,通过对这些标本的观察研究,结果发现：臀背边缘管腺的大小．数量以及触角的形态等特征变化很大;研究表明松蜕盾蚧F.pinicola、日本蜕盾蚧F.japonica、柏蜕盾蜕F.externa和霜蜕盾蚧F.prulnosa,实际上是同一个种,即松蜕盾蚧F.pinicola。     

Transient suppression of F-plasmid incompatibility in a strain of Escherichia coli

Summary The incompatibility between F plasmids is transiently suppressed in Escherichia coli strain CSH54. As a result this strain is able to maintain two F factors or an F factor and a mini-F plasmid for considerably longer periods than normal strains. When selective pressure for two markers carried by two separate Fs (or an F and mini-F) is imposed on normal strains, the two plasmids tend to form a cointegrate structure which can be detected genetically by the joint transfer of both the markers upon mating. This does not happen in CSH54; instead, the two plasmids are maintained and transferred independently. Physical evidence for the maintenance of an F and a mini-F plasmid is provided by agarose gel electrophoresis.     

禽源大肠杆菌的分离及其毒力因子的检测

从临床疑似大肠杆菌感染的病禽组织中分离到69株细菌(其中鹅源29株,鸡源40株);通过常规形态学、培养特性和生化特征的研究,确定为大肠杆菌。PCR检测表明,其中46株(66.7%)为F1 大肠杆菌,10株(14.5%)为F1 HPI 大肠杆菌,2株(2.9%)为HPI 大肠杆菌;通过比较还发现,F1菌毛和HPI在鹅源和鸡源大肠杆菌中以及不同脏器来源的菌株中具有相似的分子流行病学。O抗原鉴定结果表明鹅源大肠杆菌的O抗原型主要有O26、O78、O18、O117,鸡源大肠杆菌的O抗原型主要有O109、O24、O18、O139、O78。药敏试验表明,其中绝大多数菌株对先锋霉素V、呋喃妥因、庆大霉素敏感,对环丙沙星因菌株差异而不同,林可霉素、四环素、多粘菌素多不敏感。