Discovery of three new species of Drosophila obscura species group (Diptera: Drosophilidae) from Mount Kinabalu in Borneo

We describe three new species of the Drosophila obscura species group, D. hypercephala , D. hideakii and D. quadrangula Gao & Toda, spp. nov., all discovered from high altitudes (>1500 m) on Mt Kinabalu, Sabah, Malaysia. These are the southernmost distribution records of the D. obscura group in the Oriental Region. The three new species are each morphologically similar to different species of the Old World D. obscura species subgroup. This suggests that they are descendants of different founder species that independently colonized the tropics from the subtropical or temperate zones during the glacial ages. The new species D. hypercephala is hypercephalic not only in males but also, although less distinct, in females, unlike other hypercephalic drosophilid species in which only males are hypercephalic. This new finding of a hypercephalic species from the subgenus Sophophora , of which species are relatively easy to culture in the laboratory and include the model species such as D. melanogaster and D. pseudoobscura , will promote genetic analyses on the development and the evolution of hypercephaly.     

Revision of Palaearctic mountain midges (Diptera: Deuterophlebiidae), with phylogenetic and biogeographic analyses of world species

Abstract. The mountain midges (Diptera: Deuterophlebiidae) of the Palaearctic Region are revised to include eight species. Four new species are described: D. brachyrhina sp. nov., D.oporina sp. nov. and D. blepharis sp.nov. from the Himalayas (Assam and Sikkim), and D.bicarinata sp. nov. from southern Korea. A lectotype is designated for D.mirabilis Edwards, and a key to adult males of all Palaearctic species is provided.
Larval, pupal and adult characters were used to reconstruct the phylogenetic and biogeographic relationships of world species of Deuterophlebiidae. Based on features of the adult male, the Himalayan species D. brachyrhina and D. oporina are considered the most primitive deuterophlebiids. The Nearctic species D. inyoensis is proposed as the sister group of the remaining species. Relationships among the latter are based primarily on larval and pupal characters; however, lack of information about the immature stages of several Palaearctic species contributes to a poorly resolved phylogeny. Several alternative hypotheses are presented and discussed. All phylogenetic alternatives suggest that the Nearctic fauna originated from at least two invasions of North America.     

Thirteen new species of Dendrochilum (Orchidaceae), a new record from Burma, and a checklist of the genus in East Malesia

Pedersen, H. Æ. 1995. Thirteen new species of Dendrochilum (Orchidaceae), a new record from Burma, and a checklist of the genus in East Malesia. — Nord. J. Bot. 15: 381402. Copenhagen. ISSN 0107–055X.
Seven new species of Dendrochilum are described from the Philippines, viz. D. amesianum, D. flexuosum, D. louisianum, D. parvipapillatum, D. plocoglottoides, D. quisumbingianum and D. tetradactyliferum . The widespread species D. longifolium is recorded from Burma for the first time, and an annotated checklist of Dendrochilum in East Malesia, i.e. the Lesser Sunda Islands, Sulawesi, the Moluccas, and New Guinea is given. This list enumerates a total of 21 species, including D. cornutum and D. pallideflavens which are here recorded from East Malesia for the first time. Six species in the checklist are described as new, viz. D. ambangense, D. citrinum, D. erectila-bium, D. eymae, D. galeatum , and D. longipedicellatum .     

Rust fungi on Annonaceae II: the genus Dasyspora Berk. & M.A. Curtis

Dasyspora gregaria, the single species of the allegedly monotypic rust genus Dasyspora (Basidiomycota, Pucciniales), was investigated by light microscopy and DNA sequencing (ITS1-5.8S-ITS2 region, partial LSU and SSU of the nuclear rDNA, mt cytochrome oxidase subunit 3). Both methods indicated that D. gregaria is not a single species but can be split in 11 distinct taxa, each of which appear confined to a single Xylopia species (Annonaceae) host. Herein nine of these are described as new. Both the phylogenetic analyses and morphology show that the species are grouped into two main clades designated Dasyspora gregaria and D. winteri. The first comprises D. gregaria, the type species of the genus, which is restricted to X. cayennensis, two new species on X. aromatica, D. segregaria from northern South America and D. echinata from Brazil. The second clade is formed by D. winteri, recombined from Puccinia winteri on X. sericea, and the new species D. amazonica on X. amazonica, D. emarginatae on X. emarginata, D. frutescentis on X. frutescens, D. ferrugineae on X. frutescens var. ferruginea, D. guianensis on X. benthamii, D. mesoamericana on X. frutescens, and D. nitidae on X. nitida. Dasyspora frutescentis and D. mesoamericana were not clearly distinguishable by their morphology and host associations but differed from another in their sequences and geographic distributions. They are considered cryptic species. An identification key and the distributions are given for all recognized species. Along with molecular data we discuss the systematic position of Dasyspora in the Pucciniales.     

The phylogeny of nine species of the Drosophila obscura group inferred by the banding homologies of chromosomal regions. I. Element B

The phylogenetic relationships among nine Drosophila species belonging to the obscura group were investigated by establishing the segments displaying banding homologies in their element B (equivalent to the U element of D. subobscura). The phylogenetic ordering of the species was accomplished using overlapping inversions. Two African species, D. kitumensis and D. microlabis, were investigated. These species are homosequential for their element B gene arrangement but differ from that of D. obscura by several rearrangements. Drosophila obscura seems to be most closely related to D. subsilvestris, from which the respective element B gene arrangements differ at least by six inversions. Three species, D. obscura, D. ambigua, and D. tristis, are closely related and form a cluster. Drosophila obscura displays an element B polymorphism for a pericentric inversion for which D. ambigua is fixed for one gene arrangement and D. tristis for the other. Both D. ambigua and D. tristis share a short distal inversion in the small arm of the chromosome, and differ in this respect from D. obscura. Drosophila madeirensis, D. guanche, and D. subobscura all share the same element B gene arrangement, which is acrocentric, but metacentric in all the other species mentioned. It was found that the gene arrangements of the species from the obscura cluster seem to occupy an intermediate position between those of the species of the D. subobscura cluster and those of the African one. The data reported generally are in good agreement with information provided in the literature.     

云南鳞毛蕨属毛柄组的分类研究

本文记载了云南产鳞毛蕨属植物18种,其中新种3种,云南新分布的种3种。对一些长期混淆不清的种还进行了分类学讨论。     

Sequence Analysis of Active Mariner Elements in Natural Populations of Drosophila Simulans

Active and inactive mariner elements from natural and laboratory populations of Drosophila simulans were isolated and sequenced in order to assess their nucleotide variability and to compare them with previously isolated mariner elements from the sibling species Drosophila mauritiana and Drosophila sechellia. The active elements of D. simulans are very similar among themselves (average 99.7% nucleotide identity), suggesting that the level of mariner expression in different natural populations is largely determined by position effects, dosage effects and perhaps other factors. Furthermore, the D. simulans elements exhibit nucleotide identities of 98% or greater when compared with mariner elements from the sibling species. Parsimony analysis of mariner elements places active elements from the three species into separate groups and suggests that D. simulans is the species from which mariner elements in D. mauritiana and D. sechellia are most likely derived. This result strongly suggests that the ancestral form of mariner among these species was an active element. The two inactive mariner elements sequenced from D. simulans are very similar to the inactive peach element from D. mauritiana. The similarity may result from introgression between D. simulans and D. mauritiana or from selective constraints imposed by regulatory effects of inactive elements.     

The Phylogenetic Relationships of the Members of the DROSOPHILA ROBUSTA Group

The phylogenetic relationships among the species of the D. robusta group were investigated by the analysis of chromosomal differences. Six of the ten known members of the D. robusta group were available for the study: D. colorata and D. robusta from the United States, and D. sordidula, D. pseudosordidula, D. lacertosa, and D. moriwakii from Japan. Analysis of the metaphase chromosomes from larval ganglion cells suggests that D. moriwakii and D. colorata, with rod-shaped X-chromosomes, are the more ancestral species, while D. sordidula, D. pseudosordidula, D. robusta, and D. lacertosa, with V-shaped X-chromosomes, are derived. The ancestral position of D. colorata and D. moriwakii is further strengthened by the fact that these are the two species in the D. robusta group that are cytologically closest to D. nigromelanica of the related D. melanica group. Of the four derived species, D. sordidula was found to be the closest to the ancestral species. The phylogeny based on the analysis of the gene sequences in the homologous chromosomes agreed with that indicated by the metaphase chromosomes. Since all attempts to obtain hybrids were unsuccessful except for the cross involving D. moriwakii females and D. colorata males, photographic maps of the salivary chromosomes were used to determine homology between the chromosomes of the different species. Evidence is presented to indicate that the D. robusta group originated in Asia (Japan), and that there were two migrations to the New World, the first leading to D. robusta, and the second to D. colorata. It is suggested that the route of migrations was across the Bering Land Bridge, and further, that the migrations occurred during the period from late Oligocene to middle Miocene, 20-25 million years ago.     

Five new didymozoid trematodes (Platyhelminthes, Digenea) from Australian platycephalid fishes

Five new didymozoid trematodes are described from two species of platycephalid fish collected in Moreton Bay. Australia. Indodidymozoon moretonensis sp. n. from the buccal cavity and branchial arches and 1. suttiei sp. n. from the flesh are described from Platycephalus fuscus. Indodidymozoon lesteri sp. n. from the belly skin. I. pearsoni sp. n. from the eye, and Helicodidymozoon tortor gen. n., sp. n. from the buccal cavity. branchial arches and operculum are described from Platycephalus endrachtensis. The taxonomic composition of the genus Didymozoon is discussed. Five species. D. branchiale. D. brevicolle, D. koti, D. musculi and D. Platycephali are moved to Indodidymozoon ; the generic diagnosis of Indodidymozoon is revised. One species. D. spirale , and one species from Neometadidymozoon, N. helicis. are moved to the newly creeted Helicodidymozoon. Helicodidymozoon differs from Didymozoon, Indodidymozoon and metadidymozoon in the structure of the hindbody which is spirally twisted.     

中国( )属鱼类的研究(鲤形目:鲤科)

中国Ｄａｎ属鱼类共有７种,分布于云南南部和西南部的三大流域中。其中半线Ｄａｎ Ｄａｉｏ ｉｎｔｅｒｒｕｂｔｕｓ,红蚌Ｄｎａ Ｄ．ｋａｋｈｉｅｎｅｎｓｉｓ,缺须Ｄａｎ Ｄ．ａｐｏｇｏｎ和珍珠Ｄａｎ Ｄ．ａｌｂｏｌｉｎｅａｔｕｓ分布地伊洛瓦底江流域的大盈江和龙川江水系,布良Ｄａｎ Ｄ．ｂｒｏｗｎｉ和撞邦Ｄａｎ．Ｄ．ｓｈａｎｅｎｓｉｓ分布于萨尔温江上游的怒江水系,金线Ｄａｎ Ｄ．ｃｈｒｙｓｏｔａｅｎｉａ     

Dryopsis ( Tectariaceae) in Guangdong,China

Based on herbaria collections, three species of the fern genus Dryopsis ( Tectariaceae ) are recognized from Guangdong Province, China . They are D. heterolaena , D. mariformis and D. maximowicziana . Among the three species, D. mariformis and D. maximowicziana are new records to Guangdong . In the newly published book , Flora of Guangdong Volume VII , the name Ctenitis apiciflora (Wall . ex Mett .) Ching ( = D. apiciflora (Wall . ex Mett .) Holttum & Edwards) instead of D. heterolaena is recorded due tomisidentification . The morphological and ecological differences between D. heterolaena and D. apiciflora are discussed . A key to the three species, habitat and distribution data on the three species are given .     

广东产轴鳞蕨属(叉蕨科) 植物

基于馆藏标本的研究, 确认广东产3 种轴鳞蕨属植物: 异鳞轴鳞蕨Dryopsis heterolaena、泡鳞轴鳞蕨D. mariformis 和阔鳞轴鳞蕨D. maximowicziana , 其中泡鳞轴鳞蕨和阔鳞轴鳞蕨为广东新记录的蕨类。澄清广东不产顶果轴鳞蕨D. apiciflora , 最近出版的《广东植物志》第七卷记载的顶囊肋毛蕨Ctenitis apiciflora ( 即顶果轴鳞蕨D. apiciflora) , 系异鳞轴鳞蕨的错误鉴定。文中列有分种检索表、种的文献引证、标本引证、生境与分布资料。     

Occurrence of Monogenea in Italian freshwater fish (Po river basin)

Sixteen Monogenean species were found on the skin and gills of 10 different fish species collected from north Italian water bodies. Cleidodiscus pricei from Ictalurus melas; Dactylogyrus alatus from Alburnus alburnus; D. anchoratus, D. formosus and D. vastator from Carassius carassius; D. difformis from Scardinius erythrophthalmus; D. rutili from Rutilus aula; D. tincae and Gyrodactylus gasterostei from Tinca tinca; D. ergensi from Leuciscus cephalus and Telestes muticellus; D. vistulae and Paradiplozoon rutili from Leuciscus cephalus; D. extensus and G. katharineri from Cyprinus carpio; G. lucii and Tetraonchus monenteron from Esox lucius. All species are new records for Italy with the exception of D. difformis, D. ergensi, D. vistulae and T. monenteron.     

云南鳞毛蕨属纤维鳞毛蕨组的分类研究

本文记载云南产鳞毛蕨届纤维鳞毛蕨组植物16种,其中6种为云南新分布,1种为新改级。     

Taxonomic review of genus Diaparsis Förster (Hymenoptera: Ichneumonidae: Tersilochinae) from South Korea

Eleven species of the genus Diaparsis, including two unidentified species, are recorded from South Korea. One new species, D. koreana Khalaim, et al., sp. nov., is described, and six species, D. carinifer (Thomson), D. convexa Khalaim, D. egregia Khalaim, D. hyperae Kusigemati, D. jucunda (Holmgren) and D. neoplicator Khalaim, are recorded from this country for the first time. Diaparsis hyperae and D. pulchra are the most abundant species of the genus in the Korean fauna. A key to eleven species of Diaparsis occurring in South Korea is provided.     

广东产轴鳞蕨属(叉蕨科)植物

基于馆藏标本的研究,确认广东产3种轴鳞蕨属植物:异鳞轴鳞蕨Dryopsis heterolaena、泡鳞轴鳞蕨D·mariformis和阔鳞轴鳞蕨D·maximowicziana,其中泡鳞轴鳞蕨和阔鳞轴鳞蕨为广东新记录的蕨类。澄清广东不产顶果轴鳞蕨D·apiciflora,最近出版的《广东植物志》第七卷记载的顶囊肋毛蕨Ctenitis apiciflora(即顶果轴鳞蕨D·apiciflora) ,系异鳞轴鳞蕨的错误鉴定。文中列有分种检索表、种的文献引证、标本引证、生境与分布资料。     

Ongoing hybridization obscures phylogenetic relationships in the Drosophila subquinaria species complex

Inferring evolutionary relationships among recently diverged lineages is necessary to understand how isolating barriers produce independent lineages. Here, we investigate the phylogenetic relationships between three incompletely isolated and closely related mushroom‐feeding Drosophila species. These species form the Drosophila subquinaria species complex and consist of one Eurasian species (D. transversa) and two widespread North American species (D. subquinaria and D. recens) that are sympatric in central Canada. Although patterns of pre‐ and post‐mating isolation among these species are well characterized, previous work on their phylogenetic relationships is limited and conflicting. In this study, we generated a multi‐locus data set of 29 loci from across the genome sequenced in a population sample from each species, and then, we inferred species relationships and patterns of introgression. We find strong statistical support that D. subquinaria is paraphyletic, showing that samples from the geographic region sympatric with D. recens are most closely related to D. recens, whereas samples from the geographic region allopatric with D. recens are most closely related to D. transversa. We present several lines of evidence that both incomplete lineage sorting and gene flow are causing phylogenetic discordance. We suggest that ongoing gene flow primarily from D. recens into D. subquinaria in the sympatric part of their ranges causes phylogenetic uncertainty in the evolutionary history of these species. Our results highlight how population genetic data can be used to disentangle the sources of phylogenetic discordance among closely related species.     

Evolutionary relationships of four species of hawaiian Drosophila as measured by DNA reassociation

Four species of the Hawaiian Drosophila planitibia subgroup which are homosequential in their polytene chromosomes are resident on the islands of Molokai, Maui and Hawaii. Comparisons of DNA sequence divergence in these four have been made by hybridization of total single-copy radiolabeled tracer DNA from each of the species with excess nonlabeled DNA from each of the species, and measurement of the reduction of average melting temperature (DeltaTma) was made in 2.4 m tetraethyl ammonium chloride. The mean DeltaTma between either D. heteroneura or D. silvestris and either D. planitibia or D. differens was found to be 1.06 degrees , whereas the difference between D. planitibia and D. differens in 0.65 degrees and between D. heteroneura and D. silvestris is 0.75 degrees . These measurements taken together with the distances calculated from isozyme studies, chromosomal relationships, as well as the island locations indicate that the ancestor of these species diverged from other planitibia subgroup flies on Molokai [age 1.8 million years before present, (My BP)]. We hypothesize that one line became the present-day D. differens and diverged probably at the time of formation of East Maui (0.8-1 My BP) to form the species D. planitibia. Flies from the other line migrated to Hawaii soon after its formation (0.7 My BP) to form the two species D. heteroneura and D. silvestris.