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1.
中国南海与作为海洋生物多样性中心的印太珊瑚大三角区具有一定的环境与生物连通性,其生境多样,为各种生物类群提供了有利的热带和亚热带海洋环境栖息地,具有极其丰富的生物多样性,是中国海洋生物多样性热点地区,也是重要的海洋生物多样性资源宝库。海洋细菌在生态系统循环中发挥重要作用,通过对南海海洋深层水细菌的探测,可以更加清晰地了解西太平洋和印太交汇区的生物多样性。研究基于参加开放项目获得的南海的深海站点水样,采用高通量测序进行了16S rRNA基因序列测序,分析了南海深海海水细菌群落多样性、菌群构成和多样性特征。该站点南海海洋深层水测定的细菌分属于15门、20纲、24目、86科、140属、150种,表明该区海洋深层水细菌群落丰富。测得优势菌属包括类芽胞杆菌属(Paenibacillus)、芽孢杆菌属(Bacillus)、海旋菌属(Thalassospira)、噬甲基菌属(Methylophaga)、拟杆菌属(Bacteroides)、纤细芽胞杆菌属(Gracilibacillus)、海洋芽胞杆菌属(Oceanobacillus)、海源菌属(Idiomarina)、嗜油菌属(Oleiphilus)、食碱菌属(Alcanivorax),这些属的细菌在生物材料或药物、生物燃料、水处理、降解石油烃等方面均有开发利用的潜力。属内的解淀粉芽孢杆菌(Lentibacillus amyloliquefaciens)、施氏假单胞菌(Pseudomonas stutzeri)、柴油食烷菌(Alcanivorax dieselolei)、三浦半岛盐乳杆菌(Halolactibacillus miurensis)、海迪茨氏菌(Dietzia maris)可以具体鉴定到种,这些深海细菌目前已在产抗生素、生物防治、石油降解、反硝化等方面被开发利用。基于高通量测序显示出采样点南海海洋深层水细菌具有独特菌群特征和多样性,有其极为丰富的生物学开发利用价值。 相似文献
2.
A. M. Prokofiev 《Journal of Ichthyology》2008,48(10):853-859
Platygobiopsis dispar sp. nov. is described on the basis of four specimens from the South China Sea off central Vietnam. The species differs from
the only previously described species, P. akihito, in the morphology of the cephalic laterosensory system; in the distribution of scales on top of the head; in the absence
of scales at the base of the pectoral-fin; in having 11 branched rays in the second dorsal and anal fins; in the lower predorsal,
transverse, and lateral scale counts; and in other minor details. The genus Platygobiopsis is recorded for the South China Sea for the first time.
The text was submitted by the author in English. 相似文献
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4.
Wei Sun Shikun Dai Shumei Jiang Guanghua Wang Guohui Liu Houbo Wu Xiang Li 《Antonie van Leeuwenhoek》2010,98(1):65-75
In this report, the diversity of Actinobacteria associated with the marine sponge Hymeniacidon perleve collected from a remote island of the South China Sea was investigated employing classical cultivation and characterization,
16S rDNA library construction, 16S rDNA-restriction fragment length polymorphism (rDNA-RFLP) and phylogenetic analysis. A
total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological
characteristics for phylogenetic analysis on the basis of their 16S rRNA gene sequences. Results showed that the 24 isolates
were assigned to six genera including Salinispora, Gordonia, Mycobacterium, Nocardia, Rhodococcus and Streptomyces. This is the first report that Salinispora is present in a marine sponge from the South China Sea. Subsequently, 26 rDNA clones were selected from 191 clones in an
Actinobacteria-specific 16S rDNA library of the H. perleve sample, using the RFLP technique for sequencing and phylogenetic analysis. In total, 26 phylotypes were clustered in eight
known genera of Actinobacteria including Mycobacterium, Amycolatopsis, Arthrobacter, Brevibacterium, Microlunatus, Nocardioides, Pseudonocardia and Streptomyces. This study contributes to our understanding of actinobacterial diversity in the marine sponge H. perleve from the South China Sea. 相似文献
5.
Historical isolation during Pleistocene low sea level periods is thought to have contributed to divergence among marine basin populations across the Coral Triangle. In the Philippine archipelago, populations in the South China Sea, Sulu Sea–inland seas, and Philippine Sea‐Celebes Sea basins might have been partially isolated. Meanwhile, present‐day broadscale oceanographic circulation patterns suggest connectivity between these basins. To evaluate hypotheses regarding the influence of historical and contemporary factors on genetic structure, phylogeographic patterns based on mitochondrial control region sequences for a reef‐associated fish, Siganus fuscescens, were analysed. Three distinct lineages were recovered. One lineage was identified as the morphologically similar species Siganus canaliculatus, while two lineages are monophyletic with S. fuscescens. Clade divergence and demographic expansion in S. fuscescens occurred during the Pleistocene. A strong signal of latitudinal structure was detected (ΦCT = 0.188), driven by marked differences in clade distribution: one clade is widely distributed (clade A), while a second clade (clade B) has a restricted northern distribution. Regional structure of clade A is consistent with the basin isolation hypothesis (ΦCT = 0.040) and suggests isolation of the South China Sea (ΦCT = 0.091). Fine‐scale structure was observed in the South China Sea and south Philippine Sea, while Sulu Sea and inland seas were unstructured. Genetic structure across multiple spatial scales (archipelagic, regional, and fine‐scale within basins) suggests the influence of vicariant barriers and contemporary limits to gene flow in S. fuscescens that may be influenced by oceanographic circulation, geographical distance between available habitats, and latitudinal temperature differences. 相似文献
6.
We investigated the diversity of fungal communities in nine different deep-sea sediment samples of the South China Sea by culture-dependent methods followed by analysis of fungal internal transcribed spacer (ITS) sequences. Although 14 out of 27 identified species were reported in a previous study, 13 species were isolated from sediments of deep-sea environments for the first report. Moreover, these ITS sequences of six isolates shared 84–92 % similarity with their closest matches in GenBank, which suggested that they might be novel phylotypes of genera Ajellomyces, Podosordaria, Torula, and Xylaria. The antimicrobial activities of these fungal isolates were explored using a double-layer technique. A relatively high proportion (56 %) of fungal isolates exhibited antimicrobial activity against at least one pathogenic bacterium or fungus among four marine pathogenic microbes (Micrococcus luteus, Pseudoaltermonas piscida, Aspergerillus versicolor, and A. sydowii). Out of these antimicrobial fungi, the genera Arthrinium, Aspergillus, and Penicillium exhibited antibacterial and antifungal activities, while genus Aureobasidium displayed only antibacterial activity, and genera Acremonium, Cladosporium, Geomyces, and Phaeosphaeriopsis displayed only antifungal activity. To our knowledge, this is the first report to investigate the diversity and antimicrobial activity of culturable deep-sea-derived fungi in the South China Sea. These results suggest that diverse deep-sea fungi from the South China Sea are a potential source for antibiotics’ discovery and further increase the pool of fungi available for natural bioactive product screening. 相似文献
7.
Apristurus platyrhynchus and four related species were reviewed taxonomically.A. sibogae from Indonesia andA. micropterygeus from the South China Sea were valid species.A. verweyi from the Philippines andA. acanutus from the South China Sea were junior synonyms ofA. platyrhynchus. A. platyrhynchus was re-described, and its new biological information was provided. 相似文献
8.
Length–weight relationships of seven myctophid fishes (Myctophiformes: myctophidae) in the South China Sea
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Y. E. Jiang Z. Z. Chen K. Zhang J. Zhang Y. Y. Gong X. L. Kong J. T. Fan Z. J. Lin Z. Q. Fang 《Zeitschrift fur angewandte Ichthyologie》2017,33(5):1044-1046
The study included 546 specimens of seven myctophid fish species from the South China Sea (captured during June 2015 by bottom and mid‐water trawls). Length‐weight relationships (LWRs), W = aLb, were estimated using standard length. The species belong to four genera. The values for the parameter a ranged from 0.006 to 0.022, while the b values ranged from 2.710 to 3.476 (Range of r2 values: .938 to .971). According to FishBase, this study presents the first LWRs for these species, including two maximum standard lengths for Diaphus chrysorhynchus and Notoscopelus resplendens. 相似文献
9.
Sinjai Phetcharat Kattika Pattarach Pin-Chen Chen Wei-Lung Wang Shao-Lun Liu Jaruwan Mayakun 《Phycological Research》2023,71(2):116-130
The calcified green algal genus Halimeda is one of the most ecologically important but morphologically diverse seaweeds in sub-tropical and tropical waters. Because of its high morphological plasticity, the identification of Halimeda species based on morphological characters is challenging without the assistance of molecular analysis. To date, the species diversity of Halimeda in Taiwan and its overseas territories has not been investigated with the assistance of DNA sequencing, and this taxonomic knowledge gap should be filled. The present study initiates a systematic examination of the species diversity and distribution of Halimeda in Taiwan, Spratly Island, and Dongsha Atoll in the South China Sea, using DNA sequence data (plastid tufA gene and rbcL) and morphological data. Our DNA analyses revealed the presence of 10 Halimeda species (Halimeda borneensis, Halimeda cylindracea, Halimeda discoidea, Halimeda distorta, Halimeda macroloba, Halimeda minima, Halimeda opuntia, Halimeda renschii, Halimeda taiwanensis sp. nov., and Halimeda velasquezii) in the waters around Taiwan, Spratly Island, and Dongsha Atoll. The majority of the species could be readily distinguished by their morphological and anatomical characters. The proposed new species, H. taiwanensis, was differentiated not only by our algorithmic species delimitation analyses (statistical parsimony network analysis and automated barcode gap discovery), but also by its morphological features. The proposed new species differs from two externally resembled species, H. cuneata and H. discoidea, in having an undulated segment margin, the complete fusion of medullary siphons at the node, the lack of segment stalk, and the presence of a large primary utricle. Here, we present the up-to-date taxonomic account, molecular diversity, and geographical distribution of Halimeda spp. in Taiwan and associated areas of the South China Sea. Environmental factors that might drive the occurrence and latitudinal distribution of the species are also discussed. 相似文献
10.
Hau Nhu Le Narongrit Muangmai Sunisa Kheauthong Zhongmin Sun Giuseppe C. Zuccarello 《Phycological Research》2020,68(1):50-56
Flattened Gracilaria species are widely distributed along the coasts of the South China Sea with more than 20 species recorded. Within the South China Sea, Gracilaria mammillaris has only been reported from Vietnam, but this species is likely restricted to the western Atlantic. This study aimed to reevaluate the taxonomic status of Vietnamese specimens of ‘G. mammillaris’ using combined morphological and molecular data. Our data clearly indicated that Vietnamese specimens were morphologically and genetically distinct from authentic G. mammillaris from the western Atlantic, and also other described flat Gracilaria species. We, therefore, propose that specimens from Vietnam originally identified as G. mammillaris be designated as a new species, Gracilaria phuquocensis sp. nov. Morphologically, G. phuquocensis can be distinguished from other flat Gracilaria species by its small thallus size, narrower blades, many medullary layers, abundant basal nutritive filaments within mature cystocarps, and tetrasporangial nemathecium. Our rbcL sequence analyses showed that the new species was sister to Gracilaria rhodymenioides from Thailand, and these two species formed a clade with cylindrical Gracilaria species. Our study contributes to clarification of the taxonomic status of misidentified specimens attributed to the flattened Gracilaria species in the South China Sea. 相似文献
11.
A. M. Prokofiev 《Journal of Ichthyology》2010,50(9):718-727
For the first time, the genus Cocotropus is recorded from the South China Sea and in the Vietnam fauna. Two new species are described—C. astakhovi and C. eksae spp. n. They are compared in detail with 14 previously known species of this genus. The fauna of velvet-fishes of Vietnam
is revised. Previously noted for Vietnam Paraploactis sp. is attributed to C. astakhovi. 相似文献
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13.
Sponge-associated fungi represent an important source of marine natural products, but little is known about the fungal diversity
and the relationship of sponge–fungal association, especially no research on the fungal diversity in the South China Sea sponge
has been reported. In this study, a total of 111 cultivable fungi strains were isolated from two South China Sea sponges Clathrina luteoculcitella and Holoxea sp. using eight different media. Thirty-two independent representatives were selected for analysis of phylogenetic diversity
according to ARDRA and morphological characteristics. The culturable fungal communities consisted of at least 17 genera within
ten taxonomic orders of two phyla (nine orders of the phylum Ascomycota and one order of the phylum Basidiomycota) including some potential novel marine fungi. Particularly, eight genera of Apiospora, Botryosphaeria, Davidiella, Didymocrea, Lentomitella, Marasmius, Pestalotiopsis, and Rhizomucor were isolated from sponge for the first time. Sponge C. luteoculcitella has greater culturable fungal diversity than sponge Holoxea sp. Five genera of Aspergillus, Davidiella, Fusarium, Paecilomyces, and Penicillium were isolated from both sponges, while 12 genera of Apiospora, Botryosphaeria, Candida, Marasmius, Cladosporium, Didymocrea, Hypocrea, Lentomitella, Nigrospora, Pestalotiopsis, Rhizomucor, and Scopulariopsis were isolated from sponge C. luteoculcitella only. Order Eurotiales especially genera Penicillium, Aspergillus, and order Hypocreales represented the dominant culturable fungi in these two South China Sea sponges. Nigrospora oryzae strain PF18 isolated from sponge C. luteoculcitella showed a strong and broad spectrum antimicrobial activities suggesting the potential for antimicrobial compounds production. 相似文献
14.
《Harmful algae》2013
The toxigenic genus Alexandrium includes ∼30 species, but information about its biogeography at a regional scale is limited. In this study, we explored the diversity of Alexandrium along the coast of China by incubating resting cysts collected from 7 sites. A total of 231 strains of Alexandrium belonging to 7 morphospecies were found. Among them, Alexandrium andersonii, Alexandrium fraterculum, Alexandrium leei, Alexandrium pseudogonyaulax, and Alexandrium tamutum were recorded from the China Sea for the first time. Partial large subunit (LSU) and/or internal transcribed spacer region (ITS1, ITS2, and 5.8S rDNA) sequences revealed two ribotypes of Alexandrium andersonii, Alexandrium leei, and Alexandrium tamarense: Atama complex Group I and IV. Atama complex Group I was exclusively distributed in the Yellow Sea and the Bohai Sea, whereas Group IV was restricted to the East China Sea and South China Sea. Atama complex Group I produced mainly N-sulfocarbamoyl toxins (C1/C2, 61–79% of total toxins) and gonyautoxins (GTX1/4, 17–37%). Alexandrium ostenfeldii strain ASBH01 produced NEO and STX exclusively (65% and 35%, respectively). Our results support the premise that Atama complex Group I is endemic to the Asian Pacific and includes cold water species, whereas Atama complex Group IV tends to inhabit warmer waters. 相似文献
15.
Yang Tingbao Delane C. Kritsky Sun Yuan Zhang Jianying Shi Suhua N. Agrawal 《Systematic parasitology》2006,63(2):125-139
Four species of the Monogenoidea, Laticola lingaoensis n. sp., L. latesi (Tripathi, 1957) n. comb. [previously Pseudorhabdosynochus latesi (Tripathi, 1957) Kritsky & Beverley-Burton, 1986], L. paralatesi (Nagibina, 1976) n. comb. [previously Diplectanum paralatesi Nagibina, 1976] and Diplectanum penangi Liang & Leong, 1991, are reported from the gills of Lates calcarifer (Centropomidae) from the South China Sea (new geographical records for L. latesi and D. penangi). Collections from off Bathurst Island, Northern Territory, Australia, represent a new geographic record for L. paralatesi; Chilka Lake, Orissa, India, is established as the type-locality for L. latesi. Laticola n. g. (Diplectanidae) is proposed for species with a spoon-shaped copulatory organ with two to four concentric incomplete ridges in the base. Laticola lingaoensis, the type-species of Laticola, is described, and L. latesi and L. paralatesi are redescribed based on specimens from the South China Sea. Pseudorhabdosynochus monosquamodiscusi Balasuriya & Leong, 1995 and Pseudorhabdosynochus yangjiangenesis Wu & Li, 2005 are considered junior subjective synonyms of L. latesi and L. paralatesi, respectively. 相似文献
16.
Longtail tuna Thunnus tonggol (Bleeker, 1851) shows genetic partitioning across,but not within,basins of the Indo‐Pacific based on mitochondrial DNA
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D. A. Willette M. D. Santos D. Leadbitter 《Zeitschrift fur angewandte Ichthyologie》2016,32(2):318-323
Genetic stock structure is atypical in tuna species, with most species demonstrating geographically‐broad, panmictic populations. Here, genetic data suggest a distinct pattern for Thunnus tonggol across the Indo‐Pacific region. The genetic variation in the coastal tuna T. tonggol sampled from across the South China Sea was examined using the highly variable mitochondrial DNA displacement loop (D‐loop) gene region. One hundred and thirty‐nine specimens were sampled from four locations in Indonesia, Vietnam and the Philippines. Phylogenetic reconstruction of genetic relationships revealed no significant ?ST statistics and hence no population structure within the South China Sea. However, subsequent analysis with sequence data from coastal northwest India infers discrete genetic stocks between the Indian Ocean and the South China Sea. Consistent with previous genetic analyses of tuna species in the Indo‐Pacific, the findings in this study infer no population structure within each basin, but rather show a significant partitioning across the wider region. Furthermore, these results have implications for the management of the commercially valuable Thunnus tonggol across national boundaries, and thus requiring collaboration among countries to ensure its sustainable use. 相似文献
17.
The finless porpoise, Neophocaena phocaenoides, is endemic to the coastal waters of the Indo-Pacific, ranging from the Persian Gulf to Japan. Nine tetranucleotide microsatellite
loci were isolated from the finless porpoise (Neophocaena phocaenoides). Polymorphism of each locus was assessed in 39 unrelated individuals from the Yellow Sea and the South China Sea of the
Chinese waters. The number of alleles per locus varied from 2 to 11. The ranges of observed and expected heterozygosity were
0.154–0.795 and 0.146–0.839, respectively. Cross-species amplification of these loci was tested in other cetacean species.
These microsatellite markers described here will be suitable for population genetic studies of finless porpoises and other
cetacean species. 相似文献
18.
The genetic structure of mangrove species is greatly affected by their geographic history. Nine natural populations of Ceriops tagal were collected from Borneo, the Malay Peninsula, and India for this phylogeographic study. Completely different haplotype
compositions on the east versus west coasts of the Malay Peninsula were revealed using the atpB-rbcL and trnL-trnF spacers of chloroplast DNA. The average haplotype diversity (Hd) of the total population was 0.549, nucleotide diversity
(θ) was 0.030, and nucleotide difference (π) was 0.0074. The cladogram constructed by the index of population differentiation
(G
ST) clearly separated the South China Sea populations from the Indian Ocean populations. In the analysis of the minimum spanning
network, the Indian Ocean haplotypes were all derived from South China Sea haplotypes, suggesting a dispersal route of C. tagal from Southeast Asia to South Asia. The Sunda Land river system and surface currents might be accountable for the gene flow
directions in the South China Sea and Bay of Bengal, respectively. The historical geography not only affected the present
genotype distribution but also the evolution of C. tagal. These processes result in the genetic differentiation and the differentiated populations that should be considered as Management
Units (MUs) for conservation measurements instead of random forestation, which might lead to gene mixing and reduction of
genetic variability of mangrove species. According to this phylogeographic study, populations in Borneo, and east and west
Malay Peninsula that have unique genotypes should be considered as distinct MUs, and any activities resulting in gene mixing
with each other ought to be prevented. 相似文献
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20.
Belmonte Genuario Mazzocchi Maria Grazia Prusova Ira Yu. Shadrin Nicola V. 《Hydrobiologia》1994,292(1):9-15
The presence of Acartia tonsa in Black Sea waters (South Crimean coast, Ukraine) is reported for the first time. A. tonsa may be a recently introduced species in the Black Sea. Morphological features of the adults have been shown by S.E.M. 相似文献