共查询到20条相似文献,搜索用时 31 毫秒
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M. A. Sorokin S. P. Medvedev A. I. Shevchenko N. M. Slynko S. M. Zakian 《Russian Journal of Genetics》2010,46(2):249-252
The expression of genes Sox2, Klf4, Myc, Sall4, Gata6, Foxa2, Hnf4a, Cdx2, Esrrb, Hand1 in cell lines, embryos and organs of adult voles Microtus rossiaemeridionalis was studied. High resemblance of the expression patterns of these genes in the organs of adult voles, mice and humans was
demonstrated. It was established that genes Gata6, Foxa2 and Hnf4a were specifically expressed in vole extraembryonic endoderm cells, while Cdx2 and Hand1 genes, in trophoblast stem cells. This shows that these genes can be used as markers of corresponding vole cell lines. Indirect
confirmation pointing to the fact that Oct4 gene is a marker gene for epiblast cells both in the vole and mouse was obtained. 相似文献
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Jiyoung Lee Takuya Sato Narumi Ogonuki Michiko Hirose Atsuo Ogura Takehiko Ogawa Kuniya Abe 《Genesis (New York, N.Y. : 2000)》2013,51(7):498-505
Germline stem (GS) cells are stem cell lines derived from postnatal male germline cells. Remarkably, GS cells can form functional spermatozoa when transplanted into infertile host mouse testes, indicating that GS cells have spermatogonial stem cell (SSC) activity. As GS cells are the only type with SSC activity, they are most suitable for in vitro studies on male germ cell differentiation. However, GS cells can deviate from the germ cell state to become other types of cells, depending on culture conditions. Therefore, it is desirable to have a monitor system to ensure that GS cells are kept at the germ cell state in culture. Here, we established GS cell lines from neonatal testes of transgenic mice that express the fluorescent protein, Venus, whose gene expression is driven by the promoter of Mvh (mouse Vasa homolog), a gene highly specific to mammalian germ cells. This novel cell line has genuine GS cell properties equivalent to existing GS lines, including the ability to generate viable offspring. This Mvh–Venus GS cell line, to our knowledge, is the first one expressing a germ cell‐specific reporter. This valuable resource should provide new opportunities for studies on male germ cell differentiation. genesis 51:498–505. © 2013 Wiley Periodicals, Inc. 相似文献
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O. F. Gordeeva N. V. Lifantseva S. V. Khaidukov 《Russian Journal of Developmental Biology》2011,42(6):355-375
One of the main criteria of pluripotency is ability of cell lines to differentiate into the germ line. Pluripotent stem cell
lines in ground state of pluripotency differ from the lines in primed state by their ability to give rise to the mature gametes.
To understand molecular mechanisms involved in regulation of different states of pluripotency we investigated the expression
patterns of germ line specific genes in different type pluripotent stem cells and mouse and human embryonic teratocarcinoma
cells. We found that pluripotent stem cells in vitro, in blastocyst and gonocytes at stage E13.5 had similar expression patterns
in contrast to the epiblast cells at stage E6.5. Quantitative real time PCR analysis showed that Vasa/Ddx4 expression in mouse and human embryonic stem cells was significantly lower than in blastocyst and gonocytes. Moreover, Vasa/Ddx4 and E-ras expression was significantly higher in mouse embryonic stem cells than in human embryonic stem cells. Our analysis of germ
line specific gene expression in differentiating mouse embryonic stem and embryonic germ cells as well as in mouse embryonic
teratocarcinoma cells maintained under conditions promoting cell reprogramming from primed to ground state of pluripotency
(2i + LIF) revealed that only pluripotent stem cells are able to regulate the expression level of Oct4 and Vasa/Ddx4 and restore initial ground state, while in embryonic teratocarcinoma cells the expression level of these genes remained unchanged.
We suggest that expression patterns of germ lines specific genes, in particular of Vasa/Ddx4, can underlie the regulation of ground and primed states of pluripotency. 相似文献
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Downregulation and growth inhibitory effect of epithelial-type Krüppel-like transcription factor KLF4, but not KLF5, in bladder cancer 总被引:3,自引:0,他引:3
Ohnishi S Ohnami S Laub F Aoki K Suzuki K Kanai Y Haga K Asaka M Ramirez F Yoshida T 《Biochemical and biophysical research communications》2003,308(2):251-256
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