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1.
Naked eye sensing of toxic metal ions in aqueous medium using thiophene‐based ligands and its application in living cells
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Duraisamy Udhayakumari Sivalingam Suganya Sivan Velmathi Davoodbasha MubarakAli 《Journal of molecular recognition : JMR》2014,27(3):151-159
Thiophene‐based diimine (R1) and monoimine (R2) were synthesized in a single step, and their cation binding affinity was tested using colorimetric and UV–vis spectral studies. R1 selectively shows a colorimetric turn‐on response for Pb2+, Hg2+ ions and colorimetric turn‐off with Sn2+ ions, and R2 shows visual response for Cu2+ and Hg2+ over other examined metal ions in aqueous medium. R1 forms 1:1 complex with Pb2+, Hg2+, and Sn2+ and exhibits fluorescence quenching, whereas R2 shows 2:1 complex with Hg2+, Cu2+ and shows fluorescence enhancement. The structural and electronic properties of the sensors and their metal complexes were also investigated using Density Functional Theory calculations. R2 was also successfully demonstrated as a fluorescent probe for detecting Cu2+ ions in living cells. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
2.
Water‐soluble polymeric chemosensor for selective detection of Hg2+ in aqueous solution using rhodamine‐based modified poly(acrylamide–acrylic acid)
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We report the fabrication of a novel easily available turn‐on fluorescent water‐soluble polymeric chemosensor for Hg2+ ions that was simply prepared by micellar free radical polymerization of a water‐insoluble organic rhodamine‐based Hg2+‐recognizing monomer (GR6GH), with hydrophilic monomers acrylamide (AM) and acrylic acid (AA). The chemical structure of the polymeric sensor was characterized by FT‐IR and 1H NMR spectroscopy. The apparent viscosity average molecular weight Mη of poly(acrylamide–acrylic acid) [poly(AM–NaAA)] and the water‐soluble polymeric chemosensor poly(AM–NaAA–GR6GH) were 1.76 × 106 and 6.84 × 104 g/mol, respectively. Because of its amphiphilic property, the water‐soluble polymeric chemosensor can be used as a chemosensor in aqueous media. Upon addition of Hg2+ ions to an aqueous solution of poly(AM–NaAA–GR6GH), fluorescence enhancements were observed instantly. Moreover, other metal ions did not induce obvious changes to the fluorescence spectra. This approach may provide an easily measurable and inherently sensitive method for Hg2+ ion detection in environmental and biological applications. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
3.
Detection of mercury(II) by DNA templated gold nanoclusters based on forming thymidine–Hg2+–thymidine duplexes
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We have successfully synthesized gold nanoclusters (AuNCs) templated with DNA (5′‐CCCCCCCCCCCCTTTTTT‐3′), and subsequently employed the fluorescent DNA‐AuNCs as a novel probe for sensitive detections of mercury ions (Hg2+). Basically, the procedure is due to the formation of thymidine–Hg2+–thymidine duplexes between DNA‐AuNCs and Hg2+, thus leading to aggregations of DNA‐AuNCs described here occurring, and facilitating their fluorescence decrease. Significantly, this decrease of fluorescent signals permitted sensitive detection of Hg2+ in a linear range of 0.1–100 µmol L?1, with a detection limit of 0.083 µmol L?1 at a signal‐to‐noise ratio of 3. Additionally, the practicality of this probe for assaying Hg2+ in human urine and lake water samples was further validated, and showed various advantages including simplicity, selectivity, sensitivity and low cost, demonstrating its potential to broaden ways for assaying Hg2+ in real samples. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
4.
Phan Tu Quy Nguyen Khoa Hien Nguyen Chi Bao Doan Thanh Nhan Dang Van Khanh Nguyen Thi Ai Nhung Truong Quy Tung Nguyen Dinh Luyen Duong Tuan Quang 《Luminescence》2015,30(3):325-329
A new rhodamine–ethylenediamine–nitrothiourea conjugate (RT) was synthesized and its sensing property as a fluorescent chemodosimeter toward metal ions was explored in water media. Analytical results from absorption and fluorescence spectra revealed that the addition of Hg2+ ions to the aqueous solution of the chemodosimeter RT caused a distinct fluorescence OFF–ON response with a remarkable visual color change from colorless to pink; however, no clear spectral and color changes were observed from other metal ions including: Zn2+, Cu2+, Cd2+, Pb2+, Ag+, Fe2+, Cr3+, Co3+, Ni2+, Ca2+, Mg2+, K+ and Na+. The sensing results and the molecular structure suggested that a Hg2+‐induced a desulfurization reaction and cyclic guanylation of the thiourea moiety followed by ring‐opening of the rhodamine spirolactam in RT are responsible for a distinct fluorescence turn‐on signal, indicating that RT is a remarkably sensitive and selective chemodosimeter for Hg2+ ions in aqueous solution. Hg2+ within a concentration range from 0.1 to 25 μM can be determined using RT as a chemodosimeter and a detection limit of 0.04 μM is achieved. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
5.
A simple and sensitive DNA‐stablized gold nanoparticle (AuNP)‐based chemiluminescent (CL) probe for detecting mercury ion (Hg2+) in aqueous solution has been developed. The CL strategy relies upon the catalytic activity of unmodified AuNPs on the luminol–H2O2 CL reaction, and the interaction of unmodified AuNPs with DNA. The unmodified AuNPs can effectively differentiate unstructured and folded DNA. The DNA desorbs from AuNPs in the presence of Hg2+, leading to the increase in CL signal. By rationally varying the number of thymine in single‐strand oligonucleotides, the detection range could be tuned. Employing single‐strand oligonucleotides with 14 thymine in the detecting system, a sensitive linear range for Hg2+ ions from 5.0 × 10–10 to 1.0 × 10–7 mol/L and a detection limit of 2.1 × 10–10 mol/L are obtained. Changing the number of thymine to 10 and 6, it leads to a narrow detection range but a high sensitivity. Besides, DNA‐based CL nanoprobes exhibit a remarkable selectivity for Hg2+ ions over a variety of competing metal ions. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
6.
Paria Pashazadeh‐Panahi Mohammad Hasanzadeh Reza Eivazzadeh‐Keihan 《Journal of molecular recognition : JMR》2020,33(5)
In this study, d ‐penicillamine‐functionalized graphene quantum dots (DPA‐GQD) has been synthesized, which significantly increases the fluorescence intensity of GQD. We used this simple fluorescent probe for metal ions detection in human plasma samples. Designed DPA‐GQD respond to Hg2+, Cu2+, Au2+, Ag+, Co2+, Zn2+, and Pb2+ with high sensitivity. The fluorescence intensity of this probe decreased significantly in the presence of metal ions such as, Hg2+, Cu2+, Au2+, Ag+, Co2+, Zn2+, and Pb2+. In this work, a promising probe for ions monitoring was introduced. Moreover, DPA‐GQD probe has been tested in plasma samples. The functionalized DPA‐GQDs exhibits great promise as an alternative to previous fluorescent probes for bio‐labeling, sensing, and other biomedical applications in aqueous solution. 相似文献
7.
A sensitive assay of mercury using fluorescence correlation spectroscopy of gold nanoparticles
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We described a new and sensitive method for the determination of mercury ions (Hg2+) on the basis of fluorescence correlation spectroscopy (FCS) and recognition of oligonucleotides. In this assay, 30‐nm gold nanoparticles (GNPs) were modified with oligonucleotides containing thymine bases (T) as fluorescent probes, and the principle of this assay was based on the specific binding of Hg2+ by two DNA thymine bases. When two GNPs labelled with different oligonucleotides were mixed with a sample containing Hg2+, the T‐Hg2+‐T binding reaction should cause GNPs to form dimers (or oligomers), which would lead to a significant increase in the characteristic diffusion time of GNPs in the detection volume. The FCS method is a single molecule detection method and can sensitively detect the change in the characteristic diffusion time of GNPs before and after binding reactions. The quantitative analysis was performed according to the relation between the change in the characteristic diffusion time of GNPs and the concentration of Hg2+. Under optimal conditions, the linear range of this method was from 0.3 nM to 100 nM, and the detection limit was 0.14 nM for Hg2+. This new method was successfully applied for direct determination of Hg2+ levels in water and cosmetics samples. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
8.
Araghni Bhattacharya Satyajit Mahata Ashutosh Bandyopadhyay Biman B. Mandal Vadivelu Manivannan 《Luminescence》2022,37(6):883-891
The 2,4,5-tris(2-pyridyl)imidazole ( L ) molecule has been evaluated as a probe for dual sensing of Hg2+ and Cu2+ ions in EtOH/HEPES buffer medium (5 mM, pH = 7.34, 1:1, v/v). Probe L shows a good sensitive and selective turn-off response in the presence of both Hg2+ and Cu2+ ions, which is comprehensible under long UV light. The probe can detect Cu2+ ion in the pH range 3–11 and Hg2+ ion in pH 6–8. The limit of detection for Cu2+ (0.77 μM) is well under the allowable limit prescribed by the United States Environmental Protection Agency. Two metal (Cu2+/Hg2+) ions are needed per L for complete fluorescence quenching. The probe shows marked reversibility on treatment with Na2EDTA, making the protocol more economical for practical purposes. Paper strip coated with the L solution of EtOH can detect the presence of Cu2+ and Hg2+ ions in the sample using visible quenching of the fluorescence intensity. Density functional theory–time-dependent density functional theory (DFT–TDDFT) calculations support experimental observations, and d-orbitals of Cu2+/Hg2+ provide a nonradiative decay pathway. Cell imaging study using HDF and MDA-MB-231 cells also supported the viability of L in detecting Cu2+ and Hg2+ ions in living cells. 相似文献
9.
A novel multifunctional fluorescent peptide sensor based on pentapeptide dansyl‐Gly‐His‐Gly‐Gly‐Trp‐COOH (D‐P5) was designed and synthesized efficiently using Fmoc solid‐phase peptide synthesis (SPPS). This fluorescent peptide sensor shows selective and sensitive responses to Hg2+ and Cu2+ among 17 metal ions and six anions studied in N‐2‐hydroxyethylpiperazine‐N‐2‐ethane sulfonic acid (HEPES) buffer solution. The peptide probe differentiates Hg2+ and Cu2+ ions by a ‘turn‐on’ response to Hg2+ and a ‘turn‐off’ response to Cu2+. Upon addition of Hg2+ or Cu2+ ions, the sensor displayed an apparent color change that was visible under an ultraviolet lamp to the naked eye. The limits of detection (LOD) of DP‐5 were 25.0 nM for Hg2+ and 85.0 nM for Cu2+; the detection limits for Cu2+ were much lower than the drinking water maximum contaminant levels set out by the United States Environmental Protection Agency (USEPA). It is noteworthy that both D‐P5‐Hg and D‐P5‐Cu systems were also used to detect S2? successfully based on the formation of ternary complexes. The LODs of D‐P5‐Hg and D‐P5‐Cu systems for S2? were 217.0 nM and 380.0 nM, respectively. Furthermore, the binding stoichiometry, binding affinity and pH sensitivity of the probe for Hg2+ and Cu2+ were investigated. This study gives new possibilities for using a short fluorescent peptide sensor for multifunctional detection, especially for anions. 相似文献
10.
A highly selective and simple fluorescent sensor for mercury (II) ion detection based on cysteamine‐capped CdTe quantum dots synthesized by the reflux method
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Cysteamine (CA)‐capped CdTe quantum dots (QDs) (CA–CdTe QDs) were prepared by the reflux method and utilized as an efficient nano‐sized fluorescent sensor to detect mercury (II) ions (Hg2+). Under optimum conditions, the fluorescence quenching effect of CA–CdTe QDs was linear at Hg2+ concentrations in the range of 6.0–450 nmol/L. The detection limit was calculated to be 4.0 nmol/L according to the 3σ IUPAC criteria. The influence of 10‐fold Pb2+, Cu2+ and Ag+ on the determination of Hg2+ was < 7% (superior to other reports based on crude QDs). Furthermore, the detection sensitivity and selectivity were much improved relative to a sensor based on the CA–CdTe QDs probe, which was prepared using a one‐pot synthetic method. This CA–CdTe QDs sensor system represents a new feasibility to improve the detection performance of a QDs sensor by changing the synthesis method. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
11.
A simple Schiff‐base colorimetric receptor 1 was prepared. It exhibits an ‘off–on‐type’ mode with high sensitivity in the presence of Hg2+. The change in color is very easily observed by the naked eye in the presence of Hg2+, whereas other metal cations do not induce such a change. A Job plot indicated a 1 : 1 complexation stoichiometry between receptor 1 and Hg2+. The association constant for 1–Hg2+ in Tetrahydrofuran (THF) was determined to be 1.3 × 109 M‐1 using a Hill plot. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
12.
Duong Tuan Quang Nguyen Van Hop Nguyen Dinh Luyen Ha Phuong Thu Doan Yen Oanh Nguyen Khoa Hien Nguyen Van Hieu Min Hee Lee Jong Seung Kim 《Luminescence》2013,28(2):222-225
We prepared an aminothiourea‐derived Schiff base (DA) as a fluorescent chemosensor for Hg2+ ions. Addition of 1 equiv of Hg2+ ions to the aqueous solution of DA gave rise to an obvious fluorescence enhancement and the subsequent addition of more Hg2+ induced gradual fluorescence quenching. Other competing ions, including Pb2+, Cd2+, Cr3+, Zn2+, Fe2+, Co3+, Ni2+, Ca2+, Mg2+, K+ and Na+, did not induce any distinct fluorescence changes, indicating that DA can selectively detect Hg2+ ions in aqueous solution. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
13.
Patrick Carney Steven Lopez Amanda Mickley Kirill Grinberg Wenyao Zhang Zhaohua Dai 《Chirality》2011,23(10):916-920
Two multimode Hg(II) sensors, L‐MethBQA and L‐CysBQA, were obtained by fusing methionine or S‐methyl cysteine, into a bis‐quinolyl amine‐based chiral podand scaffold. Quinolyl groups serve as the fluorophore and possess nitrogen lone pairs capable of chelating metal ions. On exposure to Hg2+ or Zn2+, these sensors show signal enhancement in fluorescence. However, Cu2+ quenches their fluorescence in 30:70 acetontrile/water. L‐CysBQA complexes with Hg2+, producing an exciton‐coupled circular dichroism spectrum with the opposite sign to the one that is produced by Cu2+ or Zn2+ complexation. L‐CysBQA binds Hg2+ more strongly than Zn2+ and is shown to differentiate Hg2+ from other metal ions, such as Zn2+, Cu2+, Ni2+, and Pb2+, exceptionally well. The synergistic use of relatively soft sulfur, quinoline‐based chiral ligands and chiroptically enhanced fluorescence detection results in high sensitivity and selectivity for Hg2+. Chirality, 2011. © 2011 Wiley‐Liss, Inc. 相似文献
14.
Xiwei Li Qingxia Duan Yamin Yu Kun Wang Hanchuang Zhu Xue Zhang Caiyun Liu Pan Jia Zilu Li Wenlong Sheng Baocun Zhu 《Luminescence》2020,35(6):941-946
Mercury (Hg) is a heavy metal with high toxicity and easy migration; it can be enriched through the food chain, and cause serious threats to the natural environment and human health. So, the development of a method that can be used to detect mercury ions (Hg2+) in the environment, in cells, and in organisms is very important. Here, a new 7‐hydroxycoumarin‐derived carbonothioate‐based probe ( CC‐Hg ) was designed and synthesized for detection of Hg2+. After addition of Hg2+, a large fluorescence enhancement was observed due to the formation of 7‐hydroxyl, which reinforced the intramolecular charge transfer process. The CC‐Hg probe had good water solubility and selectivity. Moreover, the probe was able to detect Hg2+ quantitatively over the concentration range 0–2 μM and with a detection limit of 7.9 nM. Importantly, we successfully applied the probe to detect Hg2+ in water samples, in living cells, and in zebrafish. The experimental results demonstrated its potential value in practical applications. 相似文献
15.
A highly efficient and selective turn‐on fluorescent sensor for Hg2+, Ag+ and Ag nanoparticles based on a coumarin dithioate derivative
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Heba A. El‐Shekheby Ahmed H. Mangood Salem M. Hamza Ahmed S. Al‐Kady El‐Zeiny M. Ebeid 《Luminescence》2014,29(2):158-167
Based on chelation‐enhanced fluorescence, a new fluorescent coumarin derivative probe 3(1‐(7‐hydroxy‐4‐methylcoumarin)ethylidene)hydrazinecarbodithioate for Hg2+, Ag+ and Ag nanoparticles is reported. Fluorescent probe acts as a rapid and highly selective “off–on” fluorescent probe and fluorescence enhancement by factors 5 to12 times was observed upon selective complexation with Hg2+, Ag+ and Ag nanoparticles. The molar ratio plots indicated the formation of 1:1 complexes between Hg2+ and Ag+ with the probe. The linear response range covers a concentration range 0.1 × 10–5–1.9 × 10–5 mol/L, 0.1 × 10–5–2.3 × 10–5 mol/L and 0.146 × 10–12–2.63 × 10–12 mol/L for Hg2+, Ag+ and Ag nanoparticles, respectively. The interference effect of some anions and cations was also tested. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
16.
17.
The sensitive and reliable detection of Hg2+ and CN− as harsh environmental contaminants are of great importance. In view of this, a novel ‘on–off–on’ fluorescent probe based on nitrogen-rich silicon quantum dots (NR-SiQDs) has been designed for sensitive detection of Hg2+ and CN− ions in aqueous medium. NR-SiQDs were synthesized using a facile, one-step, and environment friendly procedure in the presence of 3-aminopropyl trimethoxysilane (APTMS) and ascorbic acid (AA) as precursors, with l -asparagine as a nitrogen source for surface modification. The NR-SiQDs exhibited strong fluorescence emission at 450 nm with 42.34% quantum yield, satisfactory salt tolerance, and superior photostability and pH stability. The fluorescence emission was effectively quenched using Hg2+ (turn-off) due to the formation of a nonfluorescent stable NR-SiQDs/Hg2+ complex, whereas after the addition of cyanide ions (CN−), Hg2+ ions could be leached from the surface of the NR-SiQDs and the fluorescence emission intensity of the quenched NR-SiQDs fully recovered (turn-on) due to the formation of highly stable [Hg(CN)4]2− species. After optimizing the response conditions, the obtained limits of detection were found to be 53 nM and 0.46 μM for Hg2+ and CN−, respectively. Finally, the NR-SiQD-based fluorescence probe was utilized to detect Hg2+ and CN− ions in water samples and satisfactory results were obtained, suggesting its potential application for environmental monitoring. 相似文献
18.
A carbonothioate‐based highly selective fluorescent probe with a large Stokes shift for detection of Hg2+
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Jing Xu Zhenghe Xu Zuokai Wang Caiyun Liu Baocun Zhu Xiuru Wang Kun Wang Jiangting Wang Guoqing Sang 《Luminescence》2018,33(1):219-224
Mercury (Hg) is one of the heavy metal pollutants in the environment. Even a very small amount of mercury can cause serious harm to human beings. Herein, we reported a new carbonothioate‐based fluorescent probe for the detection of Hg2+ without interference from other metal ions. This probe possessed a very large Stokes shift (192 nm), which could improve the detection sensitivity by minimizing the interferences resulted from self‐absorption or auto‐fluorescence. With the addition of Hg2+ to the probe solution, considerable fluorescence enhancement was observed. Additionally, the Hg2+ concentration of 0–16 μM and fluorescence intensity showed a good linear relationship (y = 22106× + 53108, R2 = 0.9955). Finally, the proposed probe was used to detect Hg2+ in real water samples, and its result was satisfactory. Therefore, our proposed probe would provide a promising method for the determination of Hg2+ in the environment. 相似文献
19.
Here, a simple and portable paper-based analytical device (PAD) based on the inherent capability of carbon quantum dots (CQDs) to serve as a great emitter for the bis(2,4,6-trichlorophenyl)oxalate (TCPO)–hydrogen peroxide (H₂O₂) chemiluminescence (CL) reaction is introduced for the detection of harmful mercury ions (Hg2+). The energy is transferred from the unstable reaction intermediate (1,2-dioxetanedione) to CQDs, as acceptors, and an intensive orange-red CL emission is generated at ~600 nm, which is equal to the fluorescence emission wavelength of CQDs. The analytical applicability of this system was examined for the determination of Hg2+. It was observed that Hg2+ could significantly quench the produced emission, which can be attributed to the formation of a stable and nonluminescent Hg2+–CQDs complex. Accordingly, a simple and rapid PAD was established for monitoring Hg2+, with a limit of detection of 0.04 μg ml−1. No interfering effect on the signal was found from other examined cations, indicating the acceptable specificity of the method. The designed assay was appropriately utilized to detect Hg2+ ions in cosmetic samples with high efficiency. It was characterized by its low cost, ease of use, and was facile but accurate and high selective for the detection of Hg2+ ions. In addition, the portability of this probe makes it suitable for on-site screening purposes. 相似文献
20.
D. N. Weber 《Journal of fish biology》2006,69(1):75-94
Zebrafish Danio rerio embryos were exposed to 0, 25, 50 or 75 ppb Hg2+ from 0 to 24 h post‐fertilization (hpf) then placed into Hg2+‐free water. Inductively coupled plasma‐mass spectrophotometer analysis of whole embryo Hg2+ content at 24 hpf showed a positive correlation with exposure regime (Pearson's one‐tailed, r2= 0·698, P < 0·01); at 5 days post‐hatch (dph), whole larval Hg2+ content was not detectable. Hg2+‐induced behavioural deficits in larvae were, therefore, due to changes during embryogenesis and not to residual Hg2+ in the larvae. At 5 dph, larvae were tested for responses to different frequencies but equal intensities of vibrational stimuli generated by a remotely controlled plastic hammer. Data were recorded by high‐speed videography and computer‐analysed for latency of response (ms), amplitude of the response as measured by maximum initial velocity [normalized as body (standard) lengths s?1; Vmax] and duration of behaviour from initial head movement to cessation of caudal tail movement (ms). A single mechanical stimulus resulted in behavioural outcomes that were related to embryonic Hg2+ uptake. Response latency increased with exposure level and displayed an increase of ×1·5–2·5 over control values (ANOVA, P < 0·01). The Vmax decreased with exposure level to a low of 71% of control at the highest Hg2+ concentration (ANOVA, P < 0·01). Duration of behaviour displayed a biphasic response pattern in which exposure to 0, 50 or 75 ppb Hg2+ did not result in a significantly different response yet exposure to 25 ppb Hg2+ caused a significantly longer time of active response (ANOVA, P < 0·01). Repeated stimulation (1, 2 or 4 hits s?1) resulted in a concentration‐dependent increase in response failures. Regardless of stimulation frequency, larvae exposed to 0 or 25 ppb Hg2+ as embryos maintained higher Vmax levels for longer intervals during the testing period than those exposed as embryos to either 50 or 75 ppb Hg2+. 相似文献