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1.
A number of type strains of marine bacteria, including members of the family Vibrionaceae, were cultured and examined for tetrodotoxin productivity by high-performance liquid chromatography and gas chromatography-mass spectrometry. Most of the Vibrionaceae strains produced tetrodotoxin, anhydrotetrodotoxin, or both. 相似文献
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Marine bacteria which produce tetrodotoxin 总被引:6,自引:0,他引:6
U Simidu T Noguchi D F Hwang Y Shida K Hashimoto 《Applied and environmental microbiology》1987,53(7):1714-1715
A number of type strains of marine bacteria, including members of the family Vibrionaceae, were cultured and examined for tetrodotoxin productivity by high-performance liquid chromatography and gas chromatography-mass spectrometry. Most of the Vibrionaceae strains produced tetrodotoxin, anhydrotetrodotoxin, or both. 相似文献
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No ability to produce tetrodotoxin in bacteria 总被引:3,自引:0,他引:3
Matsumura K 《Applied and environmental microbiology》2001,67(5):2393-2394
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The enzymological and genetic aspects of microbial metabolism of hydrocarbons have been extensively revealed. Such molecular information is useful for understanding the bioremediation of oil spill environments and production of hydrocarbon-specific fine chemicals. 相似文献
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Nitrite production by heterotrophic bacteria 总被引:1,自引:0,他引:1
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Hydrogen sulphide production by bacteria 总被引:9,自引:0,他引:9
CLARKE PH 《Journal of general microbiology》1953,8(3):397-407
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Ammonia production by pathogenic bacteria 总被引:6,自引:0,他引:6
Hills GM 《The Biochemical journal》1940,34(7):1057-1069
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光合细菌Rhodopseudomonas产氢的影响因子实验研究 总被引:20,自引:0,他引:20
利用光合细菌Rhodopseudomonas 8株菌株研究初期活性、光照强度、C源种类、菌株差异对生物产氢的影响表明 ,不同菌株的C源利用性有较大差异 ,但对乳酸钠都有很好的利用性 .细菌的初期活性对产氢有一定程度的影响 ,稳定生长期的细菌比对数生长期的细菌产氢活性略高 .光照强度对产氢活性的影响明显 ,在光饱和度以下 ,光照强度大则产氢速率高 .不同菌种的产氢性能有效大差异 ,从上海地区有机污染环境中分离到的RhodopseudomonasB2 1 菌株在以乳酸钠 ( 50mmol·L- 1 )为C源、谷氨酸钠 ( 1 0mol·L- 1 )为N源 ,60 0 0Lx光照、30℃下 ,最大产氢速率达到 1 4.7ml·h- 1 ·g- 1 细胞干重 . 相似文献
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During aerobic growth on glucose, several species of luminous marine bacteria exhibited an imcomplete oxidative catabolism of substrate. Pyruvate, one of the products of glucose metabolism, was excreted into the medium during exponential growth and accounted for up to 50% of the substrate carbon metabolized. When glucose was depleted from the medium, the excreted pyruvate was promptly utilized, demonstrating that the cells are capable of pyruvate catabolism. Pyruvate excretion is not a general phenomenon of carbohydrate metabolism since it does not occur during the utilization of glycerol or maltose. When cells pregrown on glycerol were exposed to glucose, they began to excrete pyruvate, even if protein synthesis was blocked with chloramphenicol. Glucose thus appears to have an effect on the activity of preexisting catabolic enzymes. 相似文献
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During aerobic growth on glucose, several species of luminous marine bacteria exhibited an imcomplete oxidative catabolism of substrate. Pyruvate, one of the products of glucose metabolism, was excreted into the medium during exponential growth and accounted for up to 50% of the substrate carbon metabolized. When glucose was depleted from the medium, the excreted pyruvate was promptly utilized, demonstrating that the cells are capable of pyruvate catabolism. Pyruvate excretion is not a general phenomenon of carbohydrate metabolism since it does not occur during the utilization of glycerol or maltose. When cells pregrown on glycerol were exposed to glucose, they began to excrete pyruvate, even if protein synthesis was blocked with chloramphenicol. Glucose thus appears to have an effect on the activity of preexisting catabolic enzymes. 相似文献
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Dr. Olukayode O. Amund Olusola A. Ogunsina 《Journal of industrial microbiology & biotechnology》1987,2(2):123-127
Summary Fermentation of cassava tubers was accompanied by a gradual decrease in pH, increased amylase activity in the steep liquor, and increased microbial load and lactic acid concentration. Amylase-producing bacterial strains associated with cassava fermentation were isolated and identified asBacillus subtilis, Bacillus licheniformis andBacillus cereus. The pH optima for the partially purified enzymes of these organisms were 7.0, 5.5 and 7.5, whilst their temperature optima were 30, 37 and 80°C. There was no significant difference in amylase activities when starch, dextrin, amylopectin, glucose and maltose were used as growth substrates. 相似文献
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Summary Four species of bacteria which includedPseudomonas
fluorescens,Vibrio
cholerae andVibrio
costicola were observed to produce glutaminase both as extracellular and intracellular fractions. Comparatively both the fractions were higher in mineral media supplemented with 1% glutamine than in nutrient broth added with or without glutamine. Extracellular glutaminase production was about 2.6–6.8 times greater than the intracellular production by all the tested strains. 相似文献
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Nitrous oxide production and methane oxidation by different ammonia-oxidizing bacteria. 总被引:7,自引:0,他引:7
Ammonia-oxidizing bacteria (AOB) are thought to contribute significantly to N2O production and methane oxidation in soils. Most of our knowledge derives from experiments with Nitrosomonas europaea, which appears to be of minor importance in most soils compared to Nitrosospira spp. We have conducted a comparative study of levels of aerobic N2O production in six phylogenetically different Nitrosospira strains newly isolated from soils and in two N. europaea and Nitrosospira multiformis type strains. The fraction of oxidized ammonium released as N2O during aerobic growth was remarkably constant (0.07 to 0.1%) for all the Nitrosospira strains, irrespective of the substrate supply (urea versus ammonium), the pH, or substrate limitation. N. europaea and Nitrosospira multiformis released similar fractions of N2O when they were supplied with ample amounts of substrates, but the fractions rose sharply (to 1 to 5%) when they were restricted by a low pH or substrate limitation. Phosphate buffer (versus HEPES) doubled the N2O release for all types of AOB. No detectable oxidation of atmospheric methane was detected. Calculations based on detection limits as well as data in the literature on CH4 oxidation by AOB bacteria prove that none of the tested strains contribute significantly to the oxidation of atmospheric CH4 in soils. 相似文献
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The purity of tritiated tetrodotoxin as determined by bioassay. 总被引:8,自引:0,他引:8
S R Levinson 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》1975,270(908):337-348
The radioactivity binding of a tritiated tetrodotoxin preparation to an electric organ particulate fraction was compared with the binding of biological activity as determined by biological assay. This comparison shows that the chromatographically homogeneous toxin contained a large proportion of radioactive impurities which bind nonspecifically to the electroplax preparation. 相似文献
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Production of the anti-listerial bacteriocin, pediocin, by lactic acid bacteria (LAB) transformed with the cloning vector pPC418 (Ped+, 9.1 kb) was influenced by composition of media and incubation temperature. Maximum pediocin production, tested against Listeria innocua, by electrotransformants of Lactococcus lactis ssp. lactis was measured in tryptone/lactose/yeast extract medium after 24 h growth at 30 °C, while incubation at 40 °C was optimum for Ped+ transformants of Streptococcus thermophilus and Enterococcus faecalis. The amount of pediocin produced by S. thermophilus in skim milk and cheese whey supplemented with 0.5% yeast extract was estimated as 51000 units ml–1 and 25000 units ml–1, respectively. Pediocin production remained essentially unchanged in reconstituted skim milk or whey media diluted up to 10-fold. The results demonstrate the capacity of recombinant strains of LAB to produce pediocin in a variety of growth media including skim milk and inexpensive cheese whey-based media, requiring minimum nutritional supplementation. 相似文献