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1.
Summary The occurrence is reported of lamellar, whorl-like structures in the cytoplasm of neuroblasts and in intercellular spaces within the neural tube in chick embryos at 4-day incubation. These configurations occur in the proximity of the nuclear envelope, and of the plasma membrane; within mitochondria; in the Golgi area, and within multivesicular protrusions of the cell surface. These structures are interpreted as the morphological expression of a resettlement process of lipoprotein materials, some of which are destined to be extruded from the cell during a phase of high metabolic activity.Dedicated to Prof. Wolfgang Bargmann on his 60th birthday.This investigation was supported in part by a grant from the C.N.R. The electron micrographs were obtained at the Centro di Microscopia Elettronica of the University of Turin. 相似文献
2.
Corticosteroid production, ACTH content in the adenohypophysis and CRF content in the median eminence were studied in vitro and in vivo in the 3-weeks-old chicken according to SAFFRAN and SCHALLY in four different parts of the day: at 6 and 12 a.m., and 6 and 12 p.m. The chicken adrenals in vitro produced only corticosterone in fluorimetrically measurable amounts, with the maximum during the day and the minimum in the evening and at night. Sensitivity to ACTH of the adrenals in vitro was higher during the day than in the evening or at night. After paper-chromatographic separation, steroid hormones equivalent to corticosterone and cortisol could be detected fluorimetrically in the plasma. The "cortisol" level was about 2-2.5 times higher than the corticosterone level. Plasma "cortisol" level displayed the peak at 6 a.m. and the minimum at 12 p.m., while corticosterone at 12 a.m. and 12 p.m., respectively. ACTH content in the adenohypophysis was lowest at 6 a.m. then increased gradually until 12 p.m. CRF level in the median eminence fell during the forenoon, was lowest at noon, to rise up to 6 p.m. and persist at a high level between 6 p.m. and 6 a.m. Thus, in the 3-week-old chicken the functioning of the hypothalamo-hypophyseal-adrenal system displays a circadian rhythm characteristic of animals showing diurnal activity. 相似文献
3.
The activity of 3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) in the isolated rat liver mitochondria changes but slightly during 24 h. In animals which were fed 10% ethanol solution for 3.5 months the enzyme activity varies within the daily cycle: maximum--at 10 p. m., minimum--at 1-4 p. m. and at 4-7 a. m.; the average daily activity gets three times lower. The cessation of the alcohol consumption makes average daily activity of the enzyme only two times higher, but the character of daily changes in the activity is different: the maximum is observed at 4-7 p. m., the minimum at 4-7 a. m. 相似文献
4.
Summary The double cone of the turtle retina was reconstructed three-dimensionally from electron micrographs of 400 serial sections. Cytoplasm of the accessory cone extends bilaterally to surround the principal cone incompletely. The cytoplasmic extensions are 0.1 m in width and 2 m in length at their longest portions. They arise at approximately the median portion of the ellipsoid (consisting of mitochondria) and terminate at the level of the outer limiting membrane. The possible function of these extensions as a clasping structure is discussed. 相似文献
5.
L Cieciura G Krakowski 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》1986,24(1):33-37
Rat pinealocytes were found to contain mitochondria in three configurational states and they were calculated at 11.00 a.m. and 11.00 p.m. Their proportions were found to change in 24 hours. Analysis of these results indicated the existence of correlation between pinealocyte bioenergetics and melatonin biosynthesis and its lack in relation to serotonin. Cell groupings with mitochondria in the same configurational state were observed, which suggest the existence of functionally differentiated zones within the pineal gland. 相似文献
6.
R Toury E Boissonneau N Stelly Y Dupuis A Berville R Perasso 《Biology of the cell / under the auspices of the European Cell Biology Organization》1985,55(1-2):71-85
Young adult rats absorbed 50 p.p.m. Cd2+ added to drinking water. After 6 weeks, 3, 6 and 9 months of treatment, the ultrastructural condition of liver, kidney and muscle was observed by electron microscopy. The choice of these tissues was determined by their differences in the capacity to accumulate Cd2+: the liver is able to concentrate a considerable amount of metal, but redistributes it throughout the entire organism, while the kidney collects it in view of its elimination. Muscle contains the least Cd2+. A general regression in mitochondria cristae accompanied by a vesiculation and a fragmentation of endoplasmic reticulum appeared simultaneously in the three tissues, at as early as 6 weeks of treatment, and extended progressively with its continuation supporting evidence of a general attack of the intracellular membrane systems. Cd2+ stimulation of membrane-degrading enzymes such as phospholipases and proteases was suggested. A concomitant diminution in glycogen stores was noted. Active synthesis of neutral lipids, especially cholesterol esters, took place in liver mitochondria of treated rats in collaboration with rough endoplasmic reticulum, and progressively generated a multiplication of electron-transparent inclusions in cytoplasm. Isolated mitochondria from liver, kidney and muscle of Cd2+-treated rats maintained partial energy coupling, but displayed a rapid early fall in cytochrome oxidase followed by a partial restoration after 6 months of treatment, and a progressively slackening of succinate dehydrogenase. Isolated vesicles of liver mitochondria inner membrane of treated rats behaved as intact mitochondria, indicating changes inside the membrane itself. Addition in vitro of the metal ion to mitochondria and also to inner membrane vesicles isolated from control rats revealed that Cd2+ was able to stop completely succinate dehydrogenase, but was totally ineffective on cytochrome oxidase. Membrane fixation of Cd2+ on the flavoprotein or SH associated with succinate dehydrogenase is proposed. Considering the close parallelism of the extensive depression of microsomal NADPH cytochrome c reductase and the rapid fall in mitochondrial cytochrome oxidase, it is suggested that an indirect inhibition process occurs, through Cd2+-induced diminution of a constituent common to all cytochromes in the cell. 相似文献
7.
Summary Endoplasmic reticulum, mitochondria, and glyoxysomes were obtained from germinating castor bean endosperm,Ricinus communis, by sucrose gradient centrifugation. When each of the three organelle preparations was diluted in 150 mM KCl and centrifuged, all of the component membrane material, measured as phospholipid, was sedimented. Also, the respective membrane enzymes, phosphorylcholine-glyceride transferase, cytochrome c oxidase and alkaline lipase were recovered. The endoplasmic reticulum retained most (60%) of its protein. The mitochondria lost almost no protein while the glyoxysomes lost much of their soluble contents.The isolated endoplasmic reticulum was in the form of vesicles, 0.02 to 1 m, lacking bound ribosomes. The size, 0.5 to 0.8 m, and the structure of the mitochondria were unchanged by the purification procedure. The mitochondria were contracted, whereas the glyoxysomes were distended. The diameter of the glyoxysomes remained 0.4 to 1.5 m, but they lost much of their internal matrix. The small amount of matrix that survived was not especially associated with the membrane. The glyoxysome membrane was about the same thickness as that of the endoplasmic reticulum, 70 Å. 相似文献
8.
Our previous studies have shown that SMAD5, an important intracellular mediator of transforming growth factor beta (TGF-beta) family, is required for normal development of the cardiovascular system in vivo. In the current study, we reported that the lack of the Smad5 gene resulted in apoptosis of cardiac myocytes in vivo. To further investigate the mechanism of the Smad5 gene in cardiomyocyte apoptosis, the embryonic stem (ES) cell differentiation system was employed. We found that the myotubes that differentiated from the homozygous Smad5ex6/ex6 mutant ES cells underwent collapse and degeneration during the late stages of in vitro differentiation, mimicking the in vivo observation. By electron microscopy, abnormal swollen mitochondria were observed in cardiomyocytes both from Smad5-deficient embryos and from ES-differentiated cells. There was also a significant reduction in mitochondrial membrane potential (Deltapsi m) and a leakage of cytochrome c from mitochondria into the cytosol of myocytes differentiated from Smad5 mutant ES cells. The expression of p53 and p21 was found to be elevated in the differentiated Smad5 mutant myocytes, and this was accompanied by an up-regulation in caspase 3 expression. These results suggest that the Smad5-mediated TGF-beta signals may protect cardiomyocytes from apoptosis by maintaining the integrity of the mitochondria, probably through suppression of p53 mediated pathways. 相似文献
9.
L Cieciura W Okrój G Krakowski W Godlewski 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》1985,23(3):175-180
Stereologic studies on diurnal variations in ultrastructural manifestations of hepatic mitochondrial function in various regions of the hepatic lobules were performed. The experiments were performed on Wistar rats in May when the liver shows the maximal content of glycogen. The materials for the studies were taken at 8 am. and 10 p.m. The stereologic examinations were done according to the method of Weibel et al. The results obtained may suggest a conversion of the orthodox hepatic mitochondria to the transitional state, that is during the glycogen minimum in the rats in May. In the morning, when the glycogen deposits increased, the pericentral mitochondria are in the orthodox state. In the evening glycogen consumption due to glycogenolysis was accompanied by conversion of the mitochondria from the orthodox state to the transitional ones, which may suggest their increased metabolic activity. 相似文献
10.
Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q 总被引:4,自引:0,他引:4
Marbois B Gin P Faull KF Poon WW Lee PT Strahan J Shepherd JN Clarke CF 《The Journal of biological chemistry》2005,280(21):20231-20238
Coenzyme Q (Q) is a redox active lipid essential for aerobic respiration in eukaryotes. In Saccharomyces cerevisiae at least eight mitochondrial polypeptides, designated Coq1-Coq8, are required for Q biosynthesis. Here we present physical evidence for a coenzyme Q-biosynthetic polypeptide complex in isolated mitochondria. Separation of digitonin-solubilized mitochondrial extracts in one- and two-dimensional Blue Native PAGE analyses shows that Coq3 and Coq4 polypeptides co-migrate as high molecular mass complexes. Similarly, gel filtration chromatography shows that Coq1p, Coq3p, Coq4p, Coq5p, and Coq6p elute in fractions higher than expected for their respective subunit molecular masses. Coq3p, Coq4p, and Coq6p coelute with an apparent molecular mass exceeding 700 kDa. Coq3 O-methyltransferase activity, a surrogate for Q biosynthesis and complex activity, also elutes at this high molecular mass. We have determined the quinone content in lipid extracts of gel filtration fractions by liquid chromatography-tandem mass spectrometry and find that demethoxy-Q(6) is enriched in fractions with Coq3p. Co-precipitation of biotinylated-Coq3 and Coq4 polypeptide from digitonin-solubilized mitochondrial extracts shows their physical association. This study identifies Coq3p and Coq4p as defining members of a Q-biosynthetic Coq polypeptide complex. 相似文献
11.
The effect of intraperitoneal administration of alpha-tocopherol (100 mg/kg weight/24 h) on ascorbate (0-0.4 mM) induced lipid peroxidation of mitochondria isolated from rat liver, cerebral hemispheres, brain stem and cerebellum was examined. The ascorbate induced light emission in hepatic mitochondria was nearly completely inhibited by alpha-tocopherol (control-group: 114.32+/-14.4; vitamin E-group: 17.45+/-2.84, c.p.m.x10(-4)). In brain mitochondria, 0.2 mM ascorbate produced the maximal chemiluminescence and significant differences among both groups were not observed. No significant differences in the chemiluminescence values between control and vitamin E treated groups were observed when the three brain regions were compared. The light emission produced by mitochondrial preparations was much higher in cerebral hemispheres than in brain stem and cerebellum. In liver and brain mitochondria from control group, the level of arachidonic acid (C20:4n6) and docosahexaenoic acid (C22:6n3) was profoundly affected. Docosahexaenoic in liver mitochondria from vitamin E group decreased by 30% upon treatment with ascorbic acid when compared with mitochondria lacking ascorbic acid. As a consequence of vitamin E treatment, a significant increase of C22:6n3 was detected in rat liver mitochondria (control-group: 6.42 +/-0.12; vitamin E-group: 10.52 +/-0.46). Ratios of the alpha-tocopherol concentrations in mitochondria from rats receiving vitamin E to those of control rats were as follows: liver, 7.79; cerebral hemispheres, 0.81; brain stem, 0.95; cerebellum, 1.05. In liver mitochondria, vitamin E shows a protector effect on oxidative damage. In addition, vitamin E concentration can be increased in hepatic but not in brain mitochondria. Lipid peroxidation mainly affected, arachidonic (C20:4n6) and docosahexaenoic (C22:6n3) acids. 相似文献
12.
Importance of 6 p.m. in Hamster Timekeeping Evidenced by Computer Analysis of Wheel-Running Activity
John J. Alleva Frederic R. Alleva James F. Pestaner Jr. Judith M. McIntyre Dennis B. Wilson 《Chronobiology international》1995,12(3):166-175
We addressed the question whether the clock signal for hamsters to become active occurs at sundown throughout summer or at some constant time after noon (p.m. time). Ten female golden hamsters housed in wheel cages in a windowless room were exposed to 24-h light/dark (LD) cycles simulating the equinoxes (LD 12: 12), when the sun sets at 6 p.m. and rises at 6 a.m., and summer (LD 14: 10, 16: 8, and 18: 6), when the sun sets after 6 p.m. and rises before 6 a.m. The onset of behavioral estrus, a mask-free phase marker of the same clock that controls wheel-running, was observed every 4 days, and wheel revolutions were recorded every 5 min for 52 days. Computer analysis of the 5-min values averaged for all 10 hamsters revealed a clear onset of running for each LD exposure. Time in the windowless room is referenced to mid-L (room “noon”) of the LD cycles. Although L-off ranged from 6 p.m. in LD 12: 12 (6 h after mid-L) to 9 p.m. in LD 18: 6, estrus began close to 4 p.m. and running close to 6 p.m. in every LD cycle. In a second study, 13 females not tested for estrus began running closer to 7 p.m. in LD 16: 8 (L-off, 8 p.m.), but when L-off was advanced to 4 p.m. they also began running on that day at 6 p.m. Testing for estrus may have made the first group of hamsters less fearful of light and therefore more responsive to a 6 p.m. clock signal to become active. It is conceivable that these nocturnal rodents voluntarily suppress, to varying degrees, overt activity from 6 p.m. standard time to sundown to avoid predators. It is noteworthy that 6 p.m. room time also marks the onset of the clock's 12-h light-sensitive period underlying hamster timekeeping. 相似文献
13.
M V Berezkin Iu N Gratsinski? V F Kudinova O A Prikazchikova 《Biulleten' eksperimental'no? biologii i meditsiny》1986,101(6):751-753
The studies of mice on the natural regimen of day and night alterations have revealed a daily rhythm of hypercapnia toxicity, with the maximum sensitivity observed at 6 a.m. and the minimum sensitivity at 6 p.m.-2 a.m. and 2 p.m.-6 p.m. The experiments on animals kept for 2 weeks-1 month on the constant light regimen have demonstrated the shift in sensitivity acrophase to 10 a.m.-4 p.m. and the prolongation of the daily period of increased sensitivity. With the animals' return to the natural regimen, a two-peak rhythm with the maximum sensitivity at 2 a.m. and 10 a.m.-2 p.m. and the minimum sensitivity at 22 p.m. and 6 a.m. was observed during the first 2 weeks-1 month. 2 months later the rhythm curve flattening was recorded, with the initial daily sensitivity rhythm to absolute hypercapnia recovering in 2 1/2 months. 相似文献
14.
Rak M Tetaud E Godard F Sagot I Salin B Duvezin-Caubet S Slonimski PP Rytka J di Rago JP 《The Journal of biological chemistry》2007,282(15):10853-10864
Atp6p is an essential subunit of the ATP synthase proton translocating domain, which is encoded by the mitochondrial DNA (mtDNA) in yeast. We have replaced the coding sequence of Atp6p gene with the non-respiratory genetic marker ARG8m. Due to the presence of ARG8m, accumulation of rho-/rho0 petites issued from large deletions in mtDNA could be restricted to 20-30% by growing the atp6 mutant in media lacking arginine. This moderate mtDNA instability created favorable conditions to investigate the consequences of a specific lack in Atp6p. Interestingly, in addition to the expected loss of ATP synthase activity, the cytochrome c oxidase respiratory enzyme steady-state level was found to be extremely low (<5%) in the atp6 mutant. We show that the cytochrome c oxidase-poor accumulation was caused by a failure in the synthesis of one of its mtDNA-encoded subunits, Cox1p, indicating that, in yeast mitochondria, Cox1p synthesis is a key target for cytochrome c oxidase abundance regulation in relation to the ATP synthase activity. We provide direct evidence showing that in the absence of Atp6p the remaining subunits of the ATP synthase can still assemble. Mitochondrial cristae were detected in the atp6 mutant, showing that neither Atp6p nor the ATP synthase activity is critical for their formation. However, the atp6 mutant exhibited unusual mitochondrial structure and distribution anomalies, presumably caused by a strong delay in inner membrane fusion. 相似文献
15.
Importance of 6 p.m. in Hamster Timekeeping Evidenced by Computer Analysis of Wheel-Running Activity
《Chronobiology international》2013,30(3):166-175
We addressed the question whether the clock signal for hamsters to become active occurs at sundown throughout summer or at some constant time after noon (p.m. time). Ten female golden hamsters housed in wheel cages in a windowless room were exposed to 24-h light/dark (LD) cycles simulating the equinoxes (LD 12: 12), when the sun sets at 6 p.m. and rises at 6 a.m., and summer (LD 14: 10, 16: 8, and 18: 6), when the sun sets after 6 p.m. and rises before 6 a.m. The onset of behavioral estrus, a mask-free phase marker of the same clock that controls wheel-running, was observed every 4 days, and wheel revolutions were recorded every 5 min for 52 days. Computer analysis of the 5-min values averaged for all 10 hamsters revealed a clear onset of running for each LD exposure. Time in the windowless room is referenced to mid-L (room “noon”) of the LD cycles. Although L-off ranged from 6 p.m. in LD 12: 12 (6 h after mid-L) to 9 p.m. in LD 18: 6, estrus began close to 4 p.m. and running close to 6 p.m. in every LD cycle. In a second study, 13 females not tested for estrus began running closer to 7 p.m. in LD 16: 8 (L-off, 8 p.m.), but when L-off was advanced to 4 p.m. they also began running on that day at 6 p.m. Testing for estrus may have made the first group of hamsters less fearful of light and therefore more responsive to a 6 p.m. clock signal to become active. It is conceivable that these nocturnal rodents voluntarily suppress, to varying degrees, overt activity from 6 p.m. standard time to sundown to avoid predators. It is noteworthy that 6 p.m. room time also marks the onset of the clock's 12-h light-sensitive period underlying hamster timekeeping. 相似文献
16.
Bortezomib, a proteasome inhibitor, shows substantial anti-tumor activity in a variety of tumor cell lines, is in phase I, II, and III clinical trials and has recently been approved for the treatment of patients with multiple myeloma. The sequence of events leading to apoptosis following proteasome inhibition by bortezomib is unclear. Bortezomib effects on components of the mitochondrial apoptotic pathway were examined: generation of reactive oxygen species (ROS), alteration in the mitochondrial membrane potential (Delta psi m), and release of cytochrome c from mitochondria. With human H460 lung cancer cells, bortezomib exposure at 0.1 microM showed induction of apoptotic cell death starting at 24 h, with increasing effects after 48-72 h of treatment. After 3-6 h, an elevation in ROS generation, an increase in Delta psi m, and the release of cytochrome c into the cytosol, were observed in a time-dependent manner. Co-incubation with rotenone and antimycin A, inhibitors of mitochondrial electron transport chain complexes I and III, or with cyclosporine A, an inhibitor of mitochondrial permeability transition pore, resulted in inhibition of bortezomib-induced ROS generation, increase in Delta psi m, and cytochrome c release. Tiron, an antioxidant agent, blocked the bortezomib-induced ROS production, Delta psi m increase, and cytochrome c release. Tiron treatment also protected against the bortezomib-induced PARP protein cleavage and cell death. Benzyloxycarbonyl-VAD-fluoromethyl ketone, an inhibitor of pan-caspase, did not alter the bortezomib-induced ROS generation and increase in Delta psi m, although it prevented bortezomib-induced poly(ADP-ribose) polymerase cleavage and apoptotic death. In PC-3 prostate carcinoma cells (with overexpression of Bcl-2), a reduction of bortezomib-induced ROS generation, Delta psi m increase was correlated with cellular resistance to bortezomib and the attenuation of drug-induced apoptosis. The transient transfection of wild type p53 in p53 null H358 cells caused stimulation of the bortezomib-induced apoptosis but failed to enhance ROS generation and Delta psi m increase. Thus ROS generation plays a critical role in the initiation of the bortezomib-induced apoptotic cascade by mediation of the disruption of Delta psi m and the release of cytochrome c from mitochondria. 相似文献
17.
Bax conformational change is a crucial step for PUMA-mediated apoptosis in human leukemia 总被引:9,自引:0,他引:9
The BH3-only protein, PUMA, plays an important role in p53-mediated apoptosis. The apoptotic effect of PUMA on the mitochondria was studied using a p53-negative, human leukemia K562 cell line. Overexpression of PUMA was accompanied by an increased Bax expression, Bax conformational change, and translocation to mitochondria. A PUMA-BH3 peptide can induce Bax conformational change, cytochrome c release, and reduction in the mitochondrial membrane potential (DeltaPsi(m)) in isolated K562 mitochondria and can be inhibited by Bcl-XL. The homo-dimer of Bax/Bax was also weakly shown after mitochondria were treated with PUMA-BH3 peptide but may not be lethal for PUMA-induced apoptosis in K562 cells. Our results suggest that PUMA-induced Bax conformational change and Bax translocation to mitochondria can be separate events and the conformational change in Bax is crucial for PUMA-induced mitochondrial dysfunction. 相似文献
18.
The effect of intraperitoneal administration of α-tocopherol (100 mg/kg weight/24 h) on ascorbate (0–0.4 mM) induced lipid peroxidation of mitochondria isolated from rat liver, cerebral hemispheres, brain stem and cerebellum was examined. The ascorbate induced light emission in hepatic mitochondria was nearly completely inhibited by α-tocopherol (control-group: 114.32±14.4; vitamin E-group: 17.45±2.84, c.p.m.×10−4). In brain mitochondria, 0.2 mM ascorbate produced the maximal chemiluminescence and significant differences among both groups were not observed. No significant differences in the chemiluminescence values between control and vitamin E treated groups were observed when the three brain regions were compared. The light emission produced by mitochondrial preparations was much higher in cerebral hemispheres than in brain stem and cerebellum. In liver and brain mitochondria from control group, the level of arachidonic acid (C20:4n6) and docosahexaenoic acid (C22:6n3) was profoundly affected. Docosahexaenoic in liver mitochondria from vitamin E group decreased by 30% upon treatment with ascorbic acid when compared with mitochondria lacking ascorbic acid. As a consequence of vitamin E treatment, a significant increase of C22:6n3 was detected in rat liver mitochondria (control-group: 6.42 ±0.12; vitamin E-group: 10.52 ±0.46). Ratios of the α-tocopherol concentrations in mitochondria from rats receiving vitamin E to those of control rats were as follows: liver, 7.79; cerebral hemispheres, 0.81; brain stem, 0.95; cerebellum, 1.05. In liver mitochondria, vitamin E shows a protector effect on oxidative damage. In addition, vitamin E concentration can be increased in hepatic but not in brain mitochondria. Lipid peroxidation mainly affected, arachidonic (C20:4n6) and docosahexaenoic (C22:6n3) acids. 相似文献
19.
The cationic potentiometric fluorescent probe 3,3'-diethylthiadicarbocyanine iodide [DiS-C2(5)] was used in synaptosomes to assess the relative contributions of plasma and mitochondrial membrane potentials (psi p and psi m, respectively) to overall fluorescence. Addition of synaptosomes to media containing 0.5 microM dye caused a decrease in fluorescence intensity due to dye accumulation, which equilibrated usually within 5 min. Depolarization of mitochondria by combined treatment with cyanide and oligomycin increased fluorescence by 42%, indicating significant prior accumulation of dye into intrasynaptosomal mitochondria. psi p was calculated to be -54 mV and was not altered significantly by prior depolarization of psi m with cyanide and oligomycin (hereafter referred to as "poisoned" synaptosomes). Similarly, the linear relationship between dye fluorescence and psi p was not altered by depolarization of psi m. Valinomycin, a K+ ionophore, caused a psi p-dependent increase in fluorescence in control (nonpoisoned) synaptosomes, but did not alter fluorescence of poisoned synaptosomes except when the extracellular concentration of K+ ([K+]e) was 2 mM, in which case valinomycin hyperpolarized psi p by about 5 mV. The pore-forming antibiotic gramicidin depolarized both psi p and psi m maximally. Under these conditions, Triton X-100 further increased fluorescence by 40%, indicating significant dye binding to synaptosomal components. In poisoned synaptosomes depolarized by 75 mM K+, gramicidin caused a decrease in fluorescence intensity (hyperpolarization of psi p). The organic solvent dimethyl sulfoxide, used as a vehicle for the hydrophobic ionophores, had voltage-dependent effects on psi p and psi m.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
20.
Summary Changes in the number and distribution of mitochondria in microspores and pollen grains during male gametogenesis inPharbitis nil were examined with Technovit sections stained with 3,3-dihexyloxacarbocyanine iodide. The number of mitochondria per microspore or pollen grain ofP. nil increased constantly and dramatically during male gametogenesis. During this process, mitochondria exhibited characteristic localizations: subpopulations of mitochondria covered the surface of the microspore and vegetative nuclei before and again just after postmeiotic mitosis I (9 and 5 days before flowering, respectively). The mitochondria also surrounded the generative nucleus 2 days after postmeiotic mitosis I (5 days before flowering), although the density of mitochondria on the nuclear surface was lower. Electron microscopy showed that the mitochondria were about 30 nm from the nuclear envelope and that each mitochondrion was located near a nuclear pore. The characteristic localization of mitochondria inP. nil pollen may serve as a model to analyze the mechanisms that control mitochondrial positioning within a cell and interactions between mitochondria and nuclei.Abbreviations DAPI
4,6-diamidino-2-phenylindole
- DiOC6
3,3-dihexyloxacarbocyanine iodide
- PM I
postmeiotic mitosis I 相似文献