共查询到20条相似文献,搜索用时 46 毫秒
1.
为分析vjbR在布鲁氏菌毒力中的作用,构建了vjbR的突变株和互补株,并分析了它们在巨噬细胞和小鼠体内的存活能力。利用同源重组的方法,用卡那抗性基因替换了16M的vjbR(BMEII1116)基因,得到了vjbR的缺失突变株16M△vjbR。将vjbR基因的ORF克隆到pMD18-T载体中,然后将其转入到突变株16M△vjbR中得到互补株16M△vjbR-C。用16M、16M△vjbR和16M△vjbR-C侵染巨噬细胞和感染小鼠,比较分析它们在巨噬细胞内的生存能力及小鼠毒力。研究结果表明vjbR突变株在巨噬细胞和小鼠体内的毒力减弱,存活能力下降,说明vjbR基因是布鲁氏菌16M的毒力相关基因,对于布鲁氏菌建立慢性感染是必要的。 相似文献
2.
目的:探讨黄芪甲苷对马兜铃酸诱导的RAW264.7细胞向M1型极化的影响,并初步探索其可能的作用机制.方法:分别采用马兜铃酸和脂多糖(LPS)刺激RAW264.7细胞24h,伴或不伴黄芪甲苷进行药物干预处理.采用细胞计数检测试剂盒-8(CCK8)检测细胞活性变化,流式细胞仪检测巨噬细胞分型,酶联免疫吸附试验(ELISA... 相似文献
3.
目的:本实验探讨人羊膜上皮细胞(human amniotic epithelial cells,h AECs)预防RAW264.7细胞在脂多糖(LPS)刺激后向M1极化的作用以及可能机制。方法:采用流式细胞仪检测细胞凋亡,划痕实验检测细胞迁移,ELISA检测细胞释放NO浓度,Real-time PCR检测白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、一氧化氮合成酶(inducible nitric oxide synthase,i NOS)、精氨酸酶1(arginase-1,Agr-1)及甘露糖受体(mannose receptor,MR又称CD206)等基因表达情况,Western blotting检测RAW264.7胞质蛋白p-IκBα以及胞核蛋白NF-κB的表达。结果:RAW264.7培养基组与h AECs条件培养基干预组的凋亡率分别为5.68%±2.3%、6.68%±2.1%(p0.05)。LPS刺激组与h AECs条件培养基干预组两组的迁移率分别为42.03±0.07%、14.71±0.04%(p0.05);LPS刺激组与h AECs干预组两组NO释放量分别为27.73±10μM、13.33±6.43μM(p0.05);Real Time-PCR结果显示,h AECs干预组M1型巨噬细胞相关基因如IL-1β、TNF-α、iNOS以及INF-β的表达显著下调,M2型巨噬细胞相关基因如Arg-1、CD206、CD36等表达上调(p0.01)。Western blotting结果显示,hAECs干预组RAW264.7中胞质蛋白p-IκBа以及胞核蛋白NF-κB的蛋白含量降低。结论:h AECs与RAW264.7预培养能有效预防LPS刺激下RAW264.7向M1极化,其机制可能是通过抑制IκBα蛋白磷酸化来降低核内NF-κB的含量,从而抑制了M1型相关基因的表达。 相似文献
4.
[目的]研究艾拉莫德(T-614)对小鼠巨噬细胞(RAW264.7)M1型极化的影响。[方法]细胞毒性实验观察3个浓度(400 g/L,800 g/L,1 200 g/L)的T-614对RAW264.7的影响,使用LPS/IFN-γ诱导RAW264.7发生M1型分化,同时进行T-614干预。流式细胞术检测RAW264.7表面F4/80+CD86+与MHCⅡ+的比例,ELISA检测细胞中IL-1β、IL-6、TNF-α的含量,RT-PCR检测细胞中IL-1β、IL-6、TNF-α、MCP-1、CD86和iNOS基因的表达,Western Blot检测细胞中MCP-1、CD86和iNOS蛋白表达水平。[结果]3个浓度T-614对未分化的巨噬细胞没有毒性;高浓度T-614降低M1巨噬细胞表面的F4/80+CD86+与MHCⅡ+比例(P<0.05),降低MCP-1、CD86和iNOS的基因表达水平与蛋白表达水平(P<0.05),降低IL-1β、IL-6、TNF-α基因表达与减少IL-1β、IL-6、TNF-α的含量(P<0.05)。[结论]T-614能抑制RAW264.7进行M1型极化,抑制MCP-1、CD86和iNOS的表达,减少IL-1β、IL-6、TNF-α的形成与分泌。 相似文献
5.
目的:探讨双氢青蒿素在体外对小鼠单核巨噬细胞RAW264.7的增殖、克隆形成、周期、凋亡和迁移的影响。方法:采用梯度浓度(2.5μg/m L, 5μg/m L, 10μg/m L, 20μg/m L)的双氢青蒿素处理RAW264.7细胞,利用CCK8实验检测双氢青蒿素对巨噬细胞增殖能力的影响,利用克隆形成实验检测双氢青蒿素对RAW264.7细胞克隆形成能力的影响,利用流式细胞术检测双氢青蒿素对RAW264.7细胞周期和凋亡的影响,利用划痕修复实验检测RAW264.7细胞迁移能力。结果:CCK8实验结果显示,双氢青蒿素可以显著抑制RAW264.7巨噬细胞的增殖能力,且抑制效果与双氢青蒿素的浓度呈正相关性。克隆形成实验结果显示,双氢青蒿素可以抑制细胞的克隆形成能力。双氢青蒿素处理使RAW264.7细胞G0/G1期比例显著升高,S期与G2/M期细胞比例显著降低。双氢青蒿素对巨噬细胞凋亡具有诱导作用,且凋亡诱导作用呈现浓度依赖的特性。划痕修复实验结果显示,双氢青蒿素可以显著抑制RAW264.7巨噬细胞的迁移能力。结论:双氢青蒿素可以导致巨噬细胞的细胞周期G0/G1阻滞,并且诱导细胞凋亡,对巨噬细胞增殖和迁移具有抑制作用。 相似文献
6.
【目的】研究植物乳杆菌JLK0142胞外多糖(EPS)对RAW264.7巨噬细胞和免疫抑制小鼠免疫活性的影响。【方法】从植物乳杆菌JLK0142培养液中分离纯化EPS,采用体外细胞培养,测定EPS对巨噬细胞增殖、吞噬活性和一氧化氮(NO)分泌的影响;采用环磷酰胺构建免疫抑制小鼠模型,灌胃不同剂量的EPS,分别测定小鼠脾脏指数、T淋巴细胞增殖活力及血清中IL-2和TNF-α水平。【结果】植物乳杆菌JLK0142胞外多糖在50–800μg/m L浓度范围内能促进正常状态RAW264.7巨噬细胞的增殖,显著提高巨噬细胞的吞噬活性及NO的分泌量;与模型组相比,EPS中、高剂量组小鼠脾脏指数和T淋巴细胞增殖活力显著提高;EPS高剂量组小鼠血清中IL-2和TNF-α含量显著提高。【结论】植物乳杆菌JLK0142胞外多糖能有效提高RAW264.7巨噬细胞的免疫活力,并拮抗环磷酰胺对小鼠免疫功能的抑制作用。 相似文献
7.
【目的】布鲁氏菌与宿主相互作用的分子机制是目前的研究热点之一,布鲁氏菌通过形成来自于内质网的布氏小体而在巨噬细胞内生存和增殖,其机制目前尚不清楚,宿主细胞内质网应激反应对病原感染和炎症的调控密切相关。揭示内质网应激反应在布鲁氏菌感染巨噬细胞中的作用以及布鲁氏菌感染对巨噬细胞分泌免疫因子的影响。【方法】构建布鲁氏菌感染RAW264.7模型,在感染后不同时间收集细胞,通过实时荧光定量PCR检测细胞内质网应激反应标志分子GRP78和CHOP,以及TNF-α、IL-1β和IL-6在m RNA水平的变化;通过Western blot和ELISA分别检测其蛋白水平的变化。【结果】布鲁氏菌感染RAW264.7细胞的最佳感染复数MOI为100?1;证明在布鲁氏菌感染4-6 h,巨噬细胞可杀伤侵入的布鲁氏菌,之后存活的细菌可在细胞内增殖;感染后24 h出现细胞凋亡,48 h出现大量细胞坏死。布鲁氏菌感染可激活RAW264.7细胞的内质网应激反应,促进GRP78的表达,同时,抑制免疫因子的分泌。【结论】内质网应激反应参与了RAW264.7对布鲁氏菌感染的调节。 相似文献
8.
结核分枝杆菌ESX-1分泌蛋白ESAT-6增强巨噬细胞吞噬功能 总被引:1,自引:0,他引:1
【目的】研究结核分枝杆菌(Mycobacterium tuberculosis)分泌蛋白ESAT-6(early secreted antigenictarget of 6 kDa)对巨噬细胞吞噬功能的影响。【方法】用重组质粒pFLAG-ESAT-6和pFLAG-EGFP转染RAW264.7细胞,经G418筛选,PCR、RT-PCR和Western blot鉴定,获得稳定表达flag-ESAT-6和flag-EGFP的RAW细胞系,然后用流式细胞术观察各稳转细胞系吞噬荧光微球的能力,并用共聚焦显微镜和菌落计数法检测稳转细胞系吞噬大肠杆菌(Escherichia coli)的能力。【结果】获得了稳定表达flag-ESAT-6的RAW-E6细胞系和表达flag-EGFP的RAW-EGFP细胞系;流式细胞术检测结果表明RAW-E6吞噬荧光微球的能力显著强于野生型细胞系RAW264.7和对照细胞系RAW-EGFP;菌落计数和激光共聚焦分析表明RAW-E6细胞系吞噬E.coli的能力也显著强于RAW264.7和RAW-EGFP。【结论】通过胞内表达发现结核分枝杆菌分泌蛋白ESAT-6能够增强巨噬细胞的吞噬功能,这将为深入理解结核分枝杆菌的致病机制提供新的思路。 相似文献
9.
《中国细胞生物学学报》2015,(9)
采用Western blot、免疫荧光和PCR检测小鼠单核巨噬细胞系RAW264.7中S1P受体1-3(S1PR1-3)的表达,然后应用吞噬实验和免疫荧光的方法检测磷酸鞘胺醇(sphingosine 1-phosphate,S1P)对其吞噬功能的调节。分别应用药理学工具和小干扰RNA的方法研究S1P调节其吞噬活性的作用机制。结果显示,小鼠单核巨噬细胞系RAW264.7表达S1PR1-3;S1P剂量依赖地增强小鼠单核巨噬细胞系RAW264.7的吞噬功能,应用S1PR2或S1PR3的拮抗剂和si RNAs可抑制S1P增强的小鼠单核巨噬细胞系RAW264.7的吞噬活性;而应用S1PR1的拮抗剂和si S1PR1并不影响S1P增强的RAW264.7的吞噬作用;且S1P可以显著上调RAW264.7中S1PR2和S1PR3的表达,但是不改变S1PR1的表达,提示S1P通过正反馈机制增强其介导的小鼠单核巨噬细胞系RAW264.7的吞噬功能。结果表明,S1P/S1PR2/3信号通路增强小鼠单核巨噬细胞吞噬活性,为单核巨噬细胞吞噬作用的分子机制调控研究提供了新线索。 相似文献
10.
肿瘤细胞能够采用不同的策略抑制人体免疫系统,使其不能正常杀伤肿瘤细胞。前期研究表明,重组人过氧化物还原酶-5 (human peroxiredoxin-5, hPRDX5)能够激活机体正常的抗肿瘤免疫反应,从而控制与清除肿瘤,然而,其确切的作用机制仍有待深入研究。本研究旨在探讨hPRDX5是否通过激活或者逆转小鼠巨噬细胞RAW264.7的极化状态,从而发挥其抗肿瘤活性。CCK8法检测结果显示,与对照组相比,不同剂量hPRDX5均能显著增强巨噬细胞活力(P<0.001);一氧化氮(nitric oxide, NO)检测试剂盒检测结果显示,hPRDX5显著增强RAW264.7细胞NO分泌水平(P<0.001);ELISA检测结果揭示,hPRDX5促进RAW264.7细胞TNF-α (P<0.01)和IL-6 (P<0.001)的分泌;流式细胞术结果揭示,hPRDX5能够升高RAW264.7细胞抗原分化簇(cluster of differentiation, CD) 80 (P<0.01)和诱导型一氧化氮合酶(inducible nitric oxide sy... 相似文献
11.
12.
载脂蛋白M(apoM)是一类在血液中主要与高密度脂蛋白(HDL)结合的载脂蛋白,呈组织特异性表达且有着众多生物学功能.体内外多种因素可从转录或转录后水平对其表达进行调控:肝细胞核因子-1α,4α(HNF-1α,4α)、肝受体同系物-1(LRH-1)、叉头框转录因子a2(Foxa2)、血小板活化因子(PAF)等可上调其表达;肝X受体(LXR)、维甲酸X受体(RXR)、法尼酯X受体(FXR)、小异源二聚体-1(SHP-1)以及绝大多数细胞因子可下调其表达,具体调节机制复杂. 结构上,apoM含有一个特征性的疏水性信号肽,可结合1 磷酸鞘氨醇(S1P)等小的生物活性脂,以此介导多项生命活动. 功能上,apoM能促进preβ-HDL的生成,并提高其一系列抗动脉粥样硬化的生物活性,如胆固醇逆向转运、抗炎、以及低密度脂蛋白(LDL)的抗氧化等.在一些糖尿病病人体内,apoM的含量也显著降低,而apoM含量的提高可以降低血糖含量,增加胰岛素分泌以及改善胰岛素抵抗,不少学者将其视为该病发生发展的一项预测指标.本文就近年来对apoM的生物学特性,特别是其表达调控机制和功能的研究进展进行综述. 相似文献
13.
M Nagai 《Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme》1987,32(6):643-649
14.
15.
《Progress in growth factor research》1992,4(2):157-170
Oncostatin M (OSM) was initially identified as a polypeptide cytokine which inhibited the in vitro growth of cells from melanoma and other solid tumors. OSM shows significant similarities in primary amino acid sequence and predicted secondary structure to leukemia inhibitory factor (LIF), ciliary neurotrophic factor (CNTF), granulocyte colony-stimulating factor (G-CSF), interleukin 6 (IL-6), and interleukin 11 (IL-11). Analysis of the genes encoding these proteins reveals a shared exon organization suggesting evolutionary descent from a common ancestral gene. Recent data indicates that OSM also shares a number of in vitro activities with other members of this cytokine family. The overlapping biological effects appear to be explained by the sharing of receptor subunits. 相似文献
16.
The ethylisocyanide equilibria of all the five known hemoglobins M, namely Hb M Iwate (alpha287 Tyrbeta2), Hb M Boston (alpha258 Tyrbeta2), Hb M Hyde Park (alpha2beta292 Tyr), Hb M Saskatoon (alpha2beta263 tyr), and Hb M Milwaukee-I (alpha2beta267 Glu), were studied both in the half-ferric and fully reduced heme states. In the half-ferric state, no heme-heme interaction was observed for Hb M Iwate, Hb M Boston, and Hb M Hyde Park, but Hb M Saskatoon and Hb M Milwaukee-I show small but definite heme-heme interaction with Hill's n of 1.3. The beta chain mutants, Hb M Hyde Park and Hb M Saskatoon, have almost normal affinity for ethylisocyanide and a normal Bohr effect, whereas the alpha chain mutants, Hb M Iwate and Hb M Boston, have abnormally low affinity and almost no Bohr effect. Hb M Milwaukee-I showed a large Bohr effect and low affinity. These results are consistent qualitatively with those on oxygen equilibria reported previously. In the fully reduced state, in which all four hemes were in the ferrous state and capable of binding ethylisocyanide distinct differences were found in the extent of heme-heme interaction. Namely, the n values for proximal histidine mutants, Hb M Iwate and Hb M Hyde Park, were 1.1 and 1.0, respectively, whereas the distal histidine mutants, Hb M Boston and Hb M Saskatoon, showed high n values of 2.4 and 1.6, respectively. Hb M Milwaukee-I also exhibited a high n value of 2.0 The ethylisocyanide affinity of the four histidine mutants was high compared with that of Hb A, while that for Hb M Milwaukee-I was almost normal. All five Hbs M had approximately normal magnitudes of Bohr effect. In the half-ferric state, the proximal and distal histidine mutants of the same chain showed similar affinity for ethylisocyanide and Bohr effect, rather different from those of the mutants of the opposite chain. These differences seem to be derived from the difference of abnormal bonding of ferric iron to tyrosine or glutamic acid. On the other hand, the reduction of iron, which abolished the abnormal bonding and made all of the chains capable of binding ligand, extinguished the differences of alpha and beta chains, and the effect of amino acid side chains close to iron on ligand binding properties became clear. Proximal histidine, which is considered to trigger the transition between the T and R states, seems to be essential to the heme-heme interaction. 相似文献
17.
18.
O.M. Barrientos E. Juárez Y. Gonzalez D.A. Castro-Villeda M. Torres S. Guzmán-Beltrán 《Letters in applied microbiology》2021,72(3):351-356
Tuberculosis (TB) is caused by Mycobacterium tuberculosis. TB is highly prevalent, characterized by the constant occurrence of drug-resistant cases, and confounded by the incidence of respiratory disease caused by non-tuberculous mycobacteria (NTB). Expanding the spectrum of drugs for the treatment of TB is indispensable. Loperamide, an antidiarrhoeal drug, enhances immune-driven antimycobacterial activity, and we aimed to evaluate its bactericidal activity against M. tuberculosis, Mycobacterium bovis BCG, Mycobacterium terrae and Mycobacterium smegmatis. Loperamide exhibited an inhibitory effect against all mycobacterial species tested, with MICs of 100 and 150 μg ml−1. Thus, loperamide is a mycobactericidal drug with potential as adjunctive therapy for TB and NTB infections. 相似文献
19.
20.
Summary The authors studied Pi polymorphism using the Separator isofocusing method with slight modification. A new Pi allele was observed. Family pedigrees confirmed co-dominant inheritance with other Pi alleles. According to the electrophoretic mobility of its isoprotein bands, and to its frequency (0.04) this new allele is considered as a fourth Pi
M subtype: Pi
M4. 相似文献