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1.
A. T. JONES 《The Annals of applied biology》1976,82(3):503-510
The effectiveness of resistance to the aphid Amphorophora rubi in restricting the spread of aphid-borne viruses was assessed in a field experiment using six genotypes of red raspberry. In one block of the experiment, the genotypes alternated with rows of virus-infected Mailing Jewel raspberry, and in the other they alternated with virus-free Mailing Jewel. During 4 years, the numbers of A. rubi and the amount of 52V virus spread in the two blocks were similar, suggesting that this virus was mostly introduced from outside the plots. Lloyd George and Mailing Jewel raspberry became heavily infested with A. rubi and were rapidly infected with raspberry leaf mottle, raspberry leaf spot and 52V viruses. Glen Clova and Norfolk Giant raspberry, which contain minor genes for resistance to A. rubi, were infested with fewer A. rubi and virus spread more slowly in these cultivars. A. rubi were rare on Mailing Orion and an East Mailing raspberry selection (888/49) which have genes A1 and A10 respectively for resistance to A.rubi, and these plants remained largely free of virus. The role of minor and major gene resistance to A. rubi in restricting virus spread is discussed. A few Macrosiphum euphorbiae and Myzus ornatus were recorded on several of the raspberry genotypes. 相似文献
2.
The distribution of distinct isolates of raspberry bushy dwarf virus (RBDV) in Rubus in England was studied. Isolates similar in Rubus host range to the Scottish type isolate (D200) were largely confined to the old red raspberry (Rubus idaeus) cv. Norfolk Giant, but were also encountered in a single plant of an unidentified raspberry cultivar and in a clump of wild R. idaeus. Outside East Mailing Research Station (EMRS) RBDV isolates with wider Rubus host ranges than that of the type isolate were found only and exclusively in hybrid berries (Loganberry, clones LY59 and L654, and Tayberry) in which infection ranged from < 1% to 100%. The significance of these findings is discussed. 相似文献
3.
C. H. CADMAN 《The Annals of applied biology》1954,41(1):207-214
Raspberry leaf mottle virus is acquired by Amphorophora rubi in feeding periods of 1/2-2 hr. Longer feeding periods cause no significant fluctuations in infectivity. During continuous feeding on healthy plants, infectivity declines rapidly after 2 hr., but persists for at least 5 hr. if aphids are transferred frequently to fresh healthy plants. The estimated probability of infection by single aphids is 1 in 20. Rarely more than 50% of test plants, Rubus occidentalis , seedlings became infected, and this is thought to be due mainly to variation in plant susceptibility. Young leaves of infected Norfolk Giant are, however, better sources of virus than older leaves. 相似文献
4.
C. H. CADMAN 《The Annals of applied biology》1952,39(1):69-77
A chlorotic veinbanding disease of raspberries is shown to be due to a virus transmissible by the aphid Amphorophora rubi Kalt. after infection feeding periods of 18 hr. or more.
This virus causes leaf symptoms, masked in hot weather, on a wide range of European and North American varieties. Symptoms on the latter are analogous to those of the red raspberry mosaic of American authors.
The names veinbanding disease and raspberry veinbanding virus are proposed. 相似文献
This virus causes leaf symptoms, masked in hot weather, on a wide range of European and North American varieties. Symptoms on the latter are analogous to those of the red raspberry mosaic of American authors.
The names veinbanding disease and raspberry veinbanding virus are proposed. 相似文献
5.
Plants of Lloyd George and Seedling M raspberry (Rubus idaeus L.) were found in eastern Scotland infected with raspberry ringspot (RRV), a virus to which these varieties were previously considered immune. Most RRV isolates from affected plants caused milder symptoms in herbaceous test plants than did the type isolates of the common Scottish and English strains. In graft-transmission tests the Lloyd George strain of RRV infected all the raspberry cultivars tested, including those immune to the common Scottish strain. No consistent differences were found between isolates of the two strains in in vitro properties or serological behaviour. Both strains were transmitted in seed of Stellaria media and in soil containing Longidorus elongatus. Possible reasons why the new strain is uncommon in Scotland are discussed. 相似文献
6.
D.J. BARBARA A.T. JONES† S.J. HENDERSON† S.C. WILSON V.H. KNIGHT 《The Annals of applied biology》1984,105(1):49-54
Isolates of raspberry bushy dwarf virus (RBDV) occurring in the field at East Mailing Research Station (EMRS), and an isolate from raspberry seed imported from the USSR, were found to differ from the Scottish type isolate (D200) of RBDV in that they infected red raspberry cultivars that are resistant, possibly immune, to isolate D200. Of several red raspberry, blackberry and hybrid berry cultivars and EMRS raspberry selections graft-inoculated with these recently discovered RBDV isolates only two raspberry cvs (Haida and Rannaya Sladkaya) and one EMRS selection did not become infected. Differences in the conclusions reached in two previous studies on the inheritance of resistance to RBDV in raspberry can be explained by the use of virus isolates that differed in Rubus host range. 相似文献
7.
RASPBERRY YELLOW DWARF, A SOIL-BORNE VIRUS 总被引:8,自引:0,他引:8
B. D. HARRISON 《The Annals of applied biology》1958,46(2):221-229
An apparently undescribed virus, provisionally named raspberry yellow dwarf virus (RYDV), was isolated from naturally infected raspberry, strawberry, blackberry and several weed species by mechanical inoculation of sap to Chenopodium amaranticolor. The severe disease it caused in Malling Exploit raspberry usually occurred patchily in otherwise normal plantations: these patches increased in size from year to year. RYDV was differentiated from raspberry ringspot and tomato black ring viruses by the symptoms produced in C. amaranticolor , tobacco and Petunia hybrida. RYDV lost infectivity when sap was heated for 10 min. at 61° C., diluted 10-5 or kept for 15 days at 18° C. RYDV was precipitated without inactivation by acetone and by ammonium sulphate.
Isolates of RYDV from different plants and localities, and of different virulence, were identified by plant-protection and serological tests. Such tests gave no evidence that RYDV was related to raspberry ringspot, tobacco ringspot, tomato black ring or cucumber mosaic viruses.
Raspberry and sugar-beet plants became systemically infected with RYDV when grown under glass in soil from a field where the disease had occurred in raspberry plants, and where the virus persisted in the soil for 3 years after the raspberry plants were removed. RYDV seems to be widely disseminated in England but recently introduced and rare in eastern Scotland.
Like raspberry ringspot and tomato black ring viruses, RYDV causes symptoms of the ringspot type in tobacco, has a wide natural and experimental host range, is soil-borne and of local importance. Such features seem characteristic of ringspot viruses as a group. 相似文献
Isolates of RYDV from different plants and localities, and of different virulence, were identified by plant-protection and serological tests. Such tests gave no evidence that RYDV was related to raspberry ringspot, tobacco ringspot, tomato black ring or cucumber mosaic viruses.
Raspberry and sugar-beet plants became systemically infected with RYDV when grown under glass in soil from a field where the disease had occurred in raspberry plants, and where the virus persisted in the soil for 3 years after the raspberry plants were removed. RYDV seems to be widely disseminated in England but recently introduced and rare in eastern Scotland.
Like raspberry ringspot and tomato black ring viruses, RYDV causes symptoms of the ringspot type in tobacco, has a wide natural and experimental host range, is soil-borne and of local importance. Such features seem characteristic of ringspot viruses as a group. 相似文献
8.
C. H. CADMAN 《The Annals of applied biology》1952,39(4):501-508
Yellows diseases of raspberry, previously considered to be physiological disorders, have causes, presumably viruses, that are transmitted by grafting. Several can be distinguished by the severity of symptoms produced in different raspberry varieties, but whether they are caused by distinct viruses or by related strains is uncertain. Two, called mild and severe yellows, are thought not to be related to the yellow mosaic and yellows virus described in North America. 相似文献
9.
A. T. Jones A. F. Murant D. L. Jennings G. A. Wood 《The Annals of applied biology》1982,100(1):135-147
The agent of raspberry yellows disease is transmitted by grafting but not by aphids and is resistant to thermotherapy. Further studies showed that it is transmitted by inoculation of sap through seed; it is probably transmitted to plants by pollination. Raspberry bushy dwarf virus (RBDV) shares all these attributes and is known to infect all yellows-sensitive raspberry cultivars except Puyallup and Sumner; however, neither of these cultivars has been tested by graft inoculation with RBDV. RBDV commonly infects plants symptomiessly, even those of yellows-sensitive cultivars, but it induced yellows when inoculated either manually to Norfolk Giant raspberry or by grafting to a yellows-sensitive raspberry selection. The evidence suggests that RBDV is the causal agent of yellows disease but that symptom expression is greatiy dependent on genetic and environmental factors. Many red raspberry cultivars are resistant, probably immune, to the type culture of RBDV and this character was shown to be conferred by a single dominant gene designated Bu. 相似文献
10.
Extensive monitoring of the raspberry bushy dwarf virus (RBDV) in cultivated raspberry, wild raspberry and blackberry was conducted in 1994‐99. RBDV was revealed by ELISA in 31.6% of field samples, 15.7% plants in germplasm collections and in 43.8% of propagated plants. Infected cultivars were Aborigen, Balzam, Brigantina, Bulharský Rubín, Canby, Comox, F‐103, Findus, Gatineau, Glen Moy, Granát, Heritage, Lloyd George, M‐101, Mája, Meeker, Norfolk Giant, Norna, NS?‐1D‐101, Skeena, Trent, Veten, ZamatoS? and Zeva. The virus was detected in 6.5 and 6.7% of wild raspberry and wild blackberry plants, respectively, at 22.8% and 11.4% of sampled locations. Vegetatively propagated plants seem to be the main source for virus spread in cultivated raspberry, rather than naturally infested wild Rubus populations. 相似文献
11.
Thin sections of diseased raspberry (Rubus idaeus) were examined by electron microscopy. Plants of the cv. Baumforth's B and of an aphid (Amphorophora rubi)-resistant breeding selection (6820/54), both infected with raspberry vein chlorosis virus (RVCV) but not with other detectable viruses, contained large bacilliform particles c. 430 × 65 nm. Particles occurred in the cytoplasm and perinuclear space of a small proportion of xylem parenchyma cells. They had an inner core c. 25–30 nm in diameter with cross-banding of periodicity 4·5 nm, and were bounded by an outer membrane. They are probably the particles of RVCV. Plants of cv. Mailing Jewel and of a selection (M14) both showing symptoms of raspberry mosaic (veinbanding) disease contained smaller bacilliform particles c. 125 × 30 nm, which occurred singly or in clusters in the cytoplasm of a small proportion of vascular parenchyma cells. It is not known which, if any, of the viruses associated with raspberry mosaic are represented by the particles. 相似文献
12.
Electron microscopy of ultrathin sections of leaves of symptomless Himalaya Giant blackberry and of the virus indicator species, Rubus macraei, showing severe leaf curl symptoms following graft inoculation with scions from this blackberry, detected highly flexuous virus‐like particles with an unusual ‘beaded’ structure. Such particles were restricted to a few vascular cells and were distinct from P‐protein common in some such cells. This virus, provisionally named Hawaiian rubus leaf curl virus (HRLCV), symptomlessly infected a wide range of Rubus species and cultivars. Badnavirus‐like bacilliform particles were observed in some cells of a single R. macraei plant showing leaf curl symptoms following graft inoculation with the causal agent of this disease symptom from Himalaya Giant blackberry after passage through red raspberry, but not in any other material. PCR with primer sets for the badnaviruses Rubus yellow net virus and Gooseberry veinbanding associated virus, showed that no Rubus sources studied contained these viruses. However, using a sequence‐specific primer set designed from the sequence of the product generated with a badnavirus degenerate primer set, a specific product was amplified from healthy plants of all of 16 raspberry cultivars and two Rubus species, but not from 16 blackberry cultivars (including cv. Himalaya Giant). All of these sources were free from viruses known to occur in Rubus. Sequence analysis of this product showed no homology with any known badnavirus, or with any other published sequences. It seems most likely therefore that a region of the raspberry genome has been amplified using the degenerate badnavirus primer set and that it is absent from the blackberry genome. 相似文献
13.
STUDIES ON THE HOST RANGE, PROPERTIES AND MODE OF TRANSMISSION OF BEET RINGSPOT VIRUS 总被引:1,自引:0,他引:1
B. D. HARRISON 《The Annals of applied biology》1957,45(3):462-472
An apparently undescribed mechanically transmissible virus has been named beet ringspot virus (BRV). It occurs naturally in Scotland in sugar-beet, turnip, swede, potato and many kinds of weed plants. BRV is readily distinguished from raspberry ringspot virus by the symptoms produced in Chenopodium amaranticolor , French bean, tobacco and Petunia hybrida plants. BRV lost infectivity when heated for 10 min. at 63°C. but not at 60°C.: at 20°C. its longevity in vitro was about 3 weeks. BRV was precipitated by ammonium sulphate, ethanol and acetone.
Protection experiments with tobacco plants, and serological tests, gave no evidence that BRV was related to tobacco ringspot, raspberry ringspot, potato bouquet or tobacco rattle viruses, but showed that viruses isolated from different host plants and from different localities were strains of BRV.
BRV is soil-borne: in glasshouse experiments sugar-beet, beetroot, potato, turnip, swede, French bean, Fragaria vesca , oat and wheat plants often became systemically infected when grown in soil from the site of a disease outbreak, but the virus was restricted to the roots of many infected plants. When sugar-beet seedlings were grown in virus-containing soil, BRV was first detected in their roots, where its concentration increased, before progressively increasing amounts of virus were found in the shoots.
Soils from five localities were found to contain BRV. BRV has been found only where the soil is light in texture, and often in fields where raspberry ringspot virus occurs. 相似文献
Protection experiments with tobacco plants, and serological tests, gave no evidence that BRV was related to tobacco ringspot, raspberry ringspot, potato bouquet or tobacco rattle viruses, but showed that viruses isolated from different host plants and from different localities were strains of BRV.
BRV is soil-borne: in glasshouse experiments sugar-beet, beetroot, potato, turnip, swede, French bean, Fragaria vesca , oat and wheat plants often became systemically infected when grown in soil from the site of a disease outbreak, but the virus was restricted to the roots of many infected plants. When sugar-beet seedlings were grown in virus-containing soil, BRV was first detected in their roots, where its concentration increased, before progressively increasing amounts of virus were found in the shoots.
Soils from five localities were found to contain BRV. BRV has been found only where the soil is light in texture, and often in fields where raspberry ringspot virus occurs. 相似文献
14.
Black raspberry necrosis virus (BRNV) induces a severe apical necrosis in black raspberry (Rubus occidentalis) but fails to induce diagnostic symptoms in red raspberry. However, BRNV infection of F1, F2 and F3 hybrids from the cross black raspberry × red raspberry induced mosaic symptoms of varying intensity but no typical apical necrosis. In a survey of 28 red raspberry cultivars, a few developed severe angular chlorotic leaf spots when infected with raspberry leaf mottle virus and a few others did so when infected with raspberry leaf spot virus. These reactions were determined by single dominant genes designated Lm and Ls respectively. The value of the different host reactions for controlling the effects and spread of these viruses is discussed. 相似文献
15.
The viability of axillary buds and the growth and potential yield of lateral shoots at nodes of red raspberry (Rubus idaeus) infected naturally by Didymella applanata or Botrytis cinerea were measured on excised nodes, decapitated nursery canes or on canes from fruiting plantations. In comparison with lesion-free nodes, buds at infected nodes were smaller and fewer of them were capable of growth when excised and ‘forced’, although the difference in growth between infected and uninfected nodes decreased during late winter. After February, those buds at infected nodes which were capable of forced growth did so as early and with a similar growth rate as those at lesion-free nodes. In April, 70% of buds at infected nodes were capable of growth compared with 94% of those at lesion-free nodes. When nursery canes of cv. Mailing Delight were decapitated above infected nodes the emergence of lateral shoots from the terminal infected node did not differ significantly from that at lesion-free nodes. On a range of farm sites in Scotland the emergence of shoots at infected nodes in the cropping region of canes was significantly poorer than from uninfected nodes but substantially better at infected nodes of cv. Glen Clova than at those of cvs Mailing Jewel and Mailing Orion. It is suggested that cv. Glen Clova is relatively tolerant of spur blight and cane botrytis. The length and potential yield of laterals which developed at infected nodes in the cropping region of canes in these three cultivars did not differ significantly from those at lesion-free nodes. In all tests there was no significant difference in growth at nodes infected by D. applanata and B. cinerea which may indicate a common mechanism for suppression of buds. 相似文献
16.
Six blackberry or hybrid berry cultivars and 19 raspberry cultivars were assessed for their infectibility with, and sensitivity to, graft inoculation with 10 distinct viruses found infecting Rubus in the UK. Cultivars were grafted with each of, two isolates of the pollen borne raspberry bushy dwarf virus (RBDV), five aphid borne viruses: black raspberry necrosis, raspberry leaf mottle (RLMV), raspberry leaf spot (RLSV), rubus yellow net and raspberry vein chlorosis (RVCV); and isolates of the nematode transmitted nepoviruses, arabis mosaic, raspberry ringspot, strawberry latent ringspot and tomato black ring. All tested cultivars were infectible with a resistance breaking isolate of RBDV but only about half of that number with the Scottish type isolate of the virus. The raspberry cvs Autumn Bliss, and occasionally Glen Garry and Glen Prosen, developed leaf yellowing symptoms following infection with RBDV, but none of the other infected cultivars showed obvious leaf symptoms when kept in a heated glasshouse during the growing season. All tested cultivars were infectible with each of the four viruses transmitted in nature by the aphid, Amphorophora idaei. Most were infected symptomlessly, but seven cultivars developed severe leaf spotting symptoms due to infection with RLMV or RLSV. All but one of the raspberry cultivars were infectible with RVCV, which is transmitted in nature by the aphid Aphis idaei, and almost all infected plants developed leaf symptoms; only one of the hybrid berry or blackberry cultivars tested was infected with RVCV. In tests with the four nepoviruses, all tested cultivars, except Tummelberry, were infectible with at least one or more of these viruses. However, cultivars responded differently to challenge inoculation with different isolates of individual nepoviruses. Several cultivars developed chlorotic leaf mottling following infection with some nepovirus isolates. The implications of these results for virus control are discussed in the light of the changing pattern of virus and virus vector incidence in the UK. 相似文献
17.
The disease, now usually called line-pattern of plum, has been described under many names in most countries where plums are grown extensively.
Naturally infected trees show widely differing Symptoms; this has two causes: (1) different varieties react differently to the same isolate of the virus, and (2) different isolates cause different symptoms in the same variety. Because the virus occurs in strains with different pathogenicities, the choice of indicator varieties is important when selecting virus-free material by transmission tests. Peach seedlings and the mazzard clone, F 12/1, were the most sensitive types found.
The line-pattern virus does not become fully systemic in some varieties of plum. In this and other respects, it resembles the viruses that cause apple mosaic; three isolates obtained from plum and two from apple produced similar Symptoms in peach and apple. It is therefore suggested that plum line-pattern and apple mosaic viruses are caused by strains of one virus. 相似文献
Naturally infected trees show widely differing Symptoms; this has two causes: (1) different varieties react differently to the same isolate of the virus, and (2) different isolates cause different symptoms in the same variety. Because the virus occurs in strains with different pathogenicities, the choice of indicator varieties is important when selecting virus-free material by transmission tests. Peach seedlings and the mazzard clone, F 12/1, were the most sensitive types found.
The line-pattern virus does not become fully systemic in some varieties of plum. In this and other respects, it resembles the viruses that cause apple mosaic; three isolates obtained from plum and two from apple produced similar Symptoms in peach and apple. It is therefore suggested that plum line-pattern and apple mosaic viruses are caused by strains of one virus. 相似文献
18.
A. T. JONES 《The Annals of applied biology》1991,118(2):331-338
After graft inoculation with rubus yellow net virus (RYNV), 12 of 34 Rubus species and cultivars developed noticeable symptoms. R. macraei developed the most conspicuous symptoms and is recommended as an improved indicator plant. In attempts to determine the cause of raspberry veinbanding mosaic, a disease in which RYNV is involved, several European and North American red raspberry cvs were graft-inoculated with RYNV and three other aphid-borne viruses, black raspberry necrosis (BRNV), raspberry leaf mottle (RLMV) and raspberry leaf spot, singly and in all combinations. In periods of up to 4 yr, classical veinbanding mosaic symptoms developed in sensitive cvs only when they contained both RYNV and RLMV. These symptoms were intensified in plants co-infected with additional viruses. Veinbanding mosaic disease did not develop in any of 11 cvs infected with RYNV + BRNV, the combination of viruses previously assumed to be responsible for this disease in Britain and North America. 相似文献
19.
The concentration of particles of black raspberry necrosis virus (BRNV), which is normally extremely low in herbaceous plants, increased about 1000-fold when Nicotiana clevelandii plants were inoculated with a mixture of BRNV and an unrelated virus, solanum nodiflorum mottle (SNMV). In sap from N. clevelandii infected with the mixed culture, BRNV infectivity survived dilution to 10?4 but not 10?5, and storage for 6 but not 8 days at 20 oC, for 6 but usually not 10 days at 4 oC and for more than 13 days at – 15 oC. When plants were inoculated with the mixed culture, BRNV induced typical symptoms in several Chenopodium species and infected several previously unreported hosts. Purified preparations of particles of the mixed culture contained only a small proportion of BRNV particles, which sedimented in sucrose density gradients as two components, one, probably non-infective, of c. 505, and the other, infective, of 120-130S. An antiserum prepared to purified particles of the mixed culture was cross-absorbed with SNMV particles and used in indirect ELISA to detect BRNV in herbaceous plants infected with the mixed culture, and also in a wide range of Rubus species, cultivars and hybrids infected naturally, by grafting or by inoculation with the aphid Amphorophora idaei. The reliability of ELISA for detecting BRNV in raspberry leaves depended on the cultivar and time of year. Some cultivars, such as Glen Clova, had low concentrations of BRNV, which was detected reliably only in late spring/early summer, whereas other cultivars, such as Lloyd George and Mailing Enterprise, had greater BRNV concentrations. In small-scale surveys in eastern Scotland, BRNV was detected by ELISA in many raspberry cvs, including some that contain major gene resistance to the vector, A. idaei; in five of nine raspberry stocks entered for the Standard grade certificate but in none of five stocks entered for the Stock Cane certificate; and in 40% of wild raspberry and 14% of wild bramble plants growing near commercial raspberry crops. The significance of these findings for the control of BRNV is discussed. 相似文献
20.
C. H. CADMAN 《The Annals of applied biology》1952,39(1):61-68
Three diseases characterized by vein chlorosis of varying grades of severity are shown to be graft transmissible to a wide range of raspberry varieties. The diseases are thought to be caused by related strains of a virus transmissible by Doralis (Aphis) idaei V. d. G. and rarely, if at all, by Amphorophora rubi Kalt. It is proposed to refer to the diseases and viruses respectively as mild, moderate and severe vein chlorosis. 相似文献