CELL POPULATION KINETICS OF EXCISED ROOTS OF PISUM SATIVUM

The cell population kinetics of excised, cultured pea roots was studied with the use of tritiated thymidine and colchicine to determine (1) the influence of excision, (2) the influence of sucrose concentration, (3) the average mitotic cycle duration, and (4) the duration of mitosis and the G₁, S, and G₂ periods of interphase.¹ The results indicate that the process of excision causes a drop in the frequency of mitotic figures when performed either at the beginning of the culture period or after 100 hours in culture. This initial decrease in frequency of cell division is independent of sucrose concentration, but the subsequent rise in frequency of division, after 12 hours in culture, is dependent upon sucrose concentration. Two per cent sucrose maintains the shortest mitotic cycle duration. The use of colchicine indicated an average cycle duration of 20 hours, whereas the use of tritiated thymidine produced an average cycle duration of 17 hours.     

GROWTH AND CELL POPULATION KINETICS OF SINGLE AND MULTIPLE KHT SARCOMAS

KHT sarcomas were implanted in syngeneic C3H/Km mice either as single intradermal tumors on the flank or as nine or ten intradermal tumors on the back. The growth curves of the single and multiple tumors were indistinguishable throughout the accurately measurable volume range. The cell population kinetics of the single and multiple tumors, examined by the per cent labeled mitoses technique, were not significantly different in any respect. The cell population kinetics in the center and periphery of the single tumors were examined separately and found to be the same.     

田间增加紫外线（UV）辐射对大豆幼苗生长和光合作用的影响

 两个增加的UV(UV-AB,280～400nm)辐射强度分别相当于大气臭氧减少3.6％和5.1％时增加的UV-B辐射。UV辐射增强明显降低大豆的株高、叶面积、干重、水分含量和叶绿素含量,大豆生长受抑程度随人工UV光源照射时间和强度增加而增强,是增加UV辐射剂量的累积效应,叶绿素b的降幅大于叶绿素a,表明UV辐射对大豆幼苗捕光色素的破坏较严重。同时,增加UV辐射还使大豆幼苗的表观光合速率、蒸腾速率、水分利用效率、气孔导度下降,作用效果与辐射强度正相关。与生长等比较,UV辐射条件下,冠／根比值减少幅度不大。分析认为,大豆幼苗生长和光合能力的下降可以使植物避免或减轻UV辐射的进一步伤害,对植物适应UV辐射有利。     

GROWING BONES ON THE COMPUTER—SOME PITFALLS OF A COMPUTER SIMULATION OF THE EFFECTS OF RADIATION ON BONE GROWTH

The linear arrangement of the proliferating cells in the growth cartilage of bone provides an apparently simple system for studying the relationship of cell kinetics to a gross endpoint, i.e. bone growth. The system has been reduced to a simple computer model which follows the effects of radiation on the growth of fifty or one hundred individual cartilage columns. When the computed data were compared with experimental curves some factors were discovered that had been omitted from the original model. A revised model proved of limited value in sorting out the relative importance of competing kinetic parameters but indicated some directions for future experimental studies.     

ACTIVITY OF TUBULOSINE ON THE KINETICS OF ROOT MERISTEM CELL POPULATION

The action of tubulosine on the mitotic cycle was studied using continuous labelling with tritiated thymidine. This alkaloid provokes a lengthening of the G₁ and S phases and a blocking of G₂ is totally reversible when the treatment is followed by recovery in normal medium. At a dose of tubulosine which induces a reversible mitostasis in the shortest possible time the lengthening of the phases of the cell cycle was estimated by three different techniques: labelled mitoses for the determination of G₂; labelling intensity for the determination of S; binucleate cells for the determination of T, and an original technique using labelling index of binucleate cells for the determination of G₁. The limits of the technique of labelled mitosis together with the interest of the technique aiming at the direct determination of G₁ in the case of a perturbed cycle are then discussed.     

THE CELL POPULATION KINETICS OF NEUTROPHILIC CELLS

Autoradiographic data for the entry of tritiated thymidine labelled cells into the post-proliferative neutrophilic cell compartments following a single injection of isotope have been analysed in terms of two cell kinetic models which differ in the assumed relationships between cell maturation and division. Comparisons with the experimental data were made in an attempt to assess the validities of the models, and kinetic parameters for the compartments of recognizable neutrophilic cells were estimated. Control mechanisms which have been proposed for the granulocyte system are discussed in terms of the kinetic models which were chosen in their determination. Although it was not possible to make a clear choice between the proposed models, preference was established for a random model which did not involve cell loss.     

UV-B辐射强对紫露草花粉母细胞的微核效应

臭氧层减薄引起紫外辐射的增强,紫露草三号用来监测辐射增加的潜在危害。在田间太阳光照下,把带有花序的紫露草枝条再给予8h不同UV-B剂量的照射,及用8μW／cm2的UV-B分别照射2、4、6和8h四种不同处理。处理后镜检花粉母细胞的微核发生情况。结果表明,UV-B照射处理花粉母细胞后其微核率显著增加。紫露草花粉母细胞的微核率可用作监测环境UV-B辐射增强的可靠指标。     

紫外线辐射增加对大豆光合作用和生长的影响

通过模拟南京地区自然光中有效紫外线Ｂ和紫外线Ａ辐射,增大辐射剂量对大豆光合作用,生长及生物量形成的影响迸行了研究。３个加强的ＵＶ辐射（０．１５,０．３５,０．７０Ｗ·ｍ－２）处理均使大豆植株矮化,抑制根、茎、叶的生长及干物质的积累。在３个ＵＶ处理中,生物效应以０．７０Ｗ·ｍ－２处理力最大,０．１５Ｗ·ｍ－２处理影响最小。ＵＶ辐射匀能使大豆叶片光合作用下降。下降幅度随ＵＶ辐射强度的增大而增大,本文还对ＵＶ影响大豆生长的可能机制进行了探讨。     

CHRONIC EFFECTS OF ULTRAVIOLET-B RADIATION ON GROWTH AND CELL VOLUME OF PHAEODACTYLUM TRICORNUTUM (BACILLARIOPHYCEAE)1

Cultures of the temperate estuarine diatom, Phaeodactylum tricornutum Bohlin (NEPCC Clone 31), were grown under ambient intensities of ultraviolet-A radiation (UVAR), photosynthetically active radiation (PAR), and various intensities of ultraviolet-B radiation (UVBR; 290–320 nm). Growth rates and cell volumes were monitored for 36 d. UVBR decreased growth rates and increased cell volumes. Sensitivity of growth to UVBR increased with time. Growth rates of P. tricornutum decreased with increasing ratios of UVBR:UVAR + PAR.     

THE EFFECTS OF POPULATION DENSITY ON GROWTH RATE IN LIMICOLARIA FLAMMEA MULLER (PULMONATA: ACHATINIDAE)

Density effects on growth and sexual maturity in laboratory-keptLimicolaria flammea MÜller are described; high densityresults in low growth, higher mortality and a delay in sexualmaturity. (Received 9 April 1991; accepted 11 June 1991)     

RADIATION EFFECTS ON CELL POPULATIONS IN THE INTESTINAL EPITHELIUM OF MICE AND ITS THEORY

Mice were exposed to 1000 R of X-rays to their trunks and sacrificed every day up to the tenth day after exposure. Cell counts were made on histological sections of the duodenum. The cell counts in the crypts were reduced to about 50% of the control value on the first day after exposure. The cell counts began to recover on the third day and an overshoot of 170% was observed on the fourth day; thereafter the crypt cell counts tended to return to the control level. The cell counts on the villi reached their minimum value on the third day after exposure. Following an overshoot on the sixth day, the villus cell counts returned to the control level by the tenth day. The above experimental results were analysed using a two-compartment model with a feedback term. A logistic proliferation was assumed for the proliferative crypt cells, while for the postmitotic villus cells the compartment was assumed to be a first in-first out type. The calculated results with this model are in general consistent with the experimental ones. The model seems to possess some essential features of the dynamics of cell renewal in the intestinal mucosa.