共查询到20条相似文献,搜索用时 15 毫秒
1.
Copper/zinc (Cu/ZnSOD) and manganese (MnSOD) superoxide dismutases which catalyze the dismutation of toxic superoxide anion, O
inf2
sup–
, to O2 and H2O2, play a major role in protecting cells from toxicity of oxidative stress. However, cells overexpressing either form of the enzyme show signs of toxicity, suggesting that too much SOD may he injurious to the cell. To elucidate the possible mechanism of this cytotoxicity, the effect of SOD on DNA and RNA strand scission was studied. High purity preparations of Cu/ZnSOD and MnSOD were tested in an in vitro assay in which DNA cleavage was measured by conversion of phage X174 supercoiled double-stranded DNA to open circular and linear forms. Both types of SOD were able to induce DNA strand scission generating single- and double-strand breaks in a process that required oxygen and the presence of fully active enzyme. The DNA strand scission could be prevented by specific anti-SOD antibodies added directly or used for immunodepletion of SOD. Requirement for oxygen and the effect of Fe(II) and Fe(III) ions suggest that cleavage of DNA may be in part mediated by hydroxyl radicals formed in Fenton-type reactions where enzyme-bound transition metals serve as a catalyst by first being reduced by superoxide and then oxidized by H2O2. Another mechanism was probably operative in this system, since in the presence of magnesium DNA cleavage by SOD was oxygen independent and not affected by sodium cyanide. It is postulated that SOD, by having a similar structure to the active center of zinc-containing nucleases, is capable of exhibiting non-specific nuclease activity causing hydrolysis of the phosphodiester bonds of DNA and RNA. Both types of SOD were shown to effectively cleave RNA. These findings may help explain the origin of pathology of certain hereditary diseases genetically linked to Cu/ZnSOD gene. 相似文献
2.
Cadmium,zinc, lead and copper inChironomus riparius (Meigen) larvae (Diptera,Chironomidae): uptake and effects 总被引:2,自引:2,他引:0
Cadmium, Zn, Pb and Cu uptake and effects in larvae ofChironomus riparius (Meigen) were studied in an integrated laboratory investigation, in which metal analyses in different instar stages, uptake kinetics and effects on development and growth were considered in three separate experiments.In short-term experiments with fourth instar larvae, it was demonstrated that only a minor portion of metals was adsorbed on the larval exoskeletons. No conclusive evidence on the uptake mechanism was found, but active uptake of trace metals seemed highly unlikely.In partial life cycle experiments, all four metals studied were readily accumulated in chironomid larvae. Uptake could be described satisfactorily utilizing a first-order one-compartment uptake model which incorporated growth. In all cases steady state conditions were approached and high uptake and elimination rate constants were estimated. Distinct differences between essential (Zn and Cu) and non-essential (Cd and Pb) metals were noted. Larval growth was significantly impaired upon exposure.Finally, long-term exposure experiments with low Cd concentrations (0.010 and 0.025 mg 1–1) resulted initially in growth impairment and high mortality in first instar stages, but surviving larvae restored growth and adults emerged even before control adults. In similar experiments with Zn (0.1 and 1.0 mg 1–1), development of larvae was significantly retarded. High Zn concentrations in larvae were noted and almost no adult midges emerged. 相似文献
3.
In alkaline calcareous waters, hydrophytes become encrusted with carbonate precipitates of a complex nature which alter bulk chemical analyses of the plants. The manganese, zinc and copper concentrations, and the ash content of two aquatic plants, Egeria densa and Potamogeton nodusus, differed considerably depending on the cleaning procedure used and, therefore, the amount of marl encrustation included in the analyses. Tapwater washed plants, retaining the marl, consistently had ash contents two to three times higher than acid-cleaned plants and exhibited higher trace metal concentrations, especially manganese. 相似文献
4.
Arredondo M Kloosterman J Núñez S Segovia F Candia V Flores S Le Blanc S Olivares M Pizarro F 《Biological trace element research》2008,125(2):109-119
It is known that heme iron and inorganic iron are absorbed differently. Heme iron is found in the diet mainly in the form
of hemoglobin and myoglobin. The mechanism of iron absorption remains uncertain. This study focused on the heme iron uptake
by Caco-2 cells from a hemoglobin digest and its response to different iron concentrations. We studied the intracellular Fe
concentration and the effect of time, K+ depletion, and cytosol acidification on apical uptake and transepithelial transport in cells incubated with different heme
Fe concentrations. Cells incubated with hemoglobin-digest showed a lower intracellular Fe concentration than cells grown with
inorganic Fe. However, uptake and transepithelial transport of Fe was higher in cells incubated with heme Fe. Heme Fe uptake
had a low V
max and K
m as compared to inorganic Fe uptake and did not compete with non-heme Fe uptake. Heme Fe uptake was inhibited in cells exposed
to K+ depletion or cytosol acidification. Heme oxygenase 1 expression increased and DMT1 expression decreased with higher heme
Fe concentrations in the media. The uptake of heme iron is a saturable and temperature-dependent process and, therefore, could
occur through a mechanism involving both a receptor and the endocytic pathway. 相似文献
5.
In a previous study, we characterized Cd–Hg interactions for uptake in human intestinal Caco-2 cells. We pursued our investigations
on metal uptake from metal mixtures, focusing on the effects of Hg on cellular homeostasis. A 4-fold higher equilibrium accumulation
value of 0.3 μmol/L 203Hg was measured in the presence of 100 μmol/L unlabeled Hg in the serum-free exposure medium without modification in the initial
uptake rate. This phenomenon was eliminated at 4∘C. Mercury induced an increase in tritiated water and [3H]mannitol uptakes for exposure times greater than 20 min. Incubations for 20 min and 30 min with 100 μmol/L Hg and 2 mmol/L
N-ethylmaleimide (NEM) resulted in a 34% and 50% reductions in cellular thiol staining, respectively, with additive effects.
Lactate dehydrogenase leakage and live/dead assays confirmed the maintenance of cell membrane integrity in Hg- or NEM-treated
cells. We conclude that Hg may alter membrane permeability and increase cell volume without any loss in cell viability. This
phenomenon is sensitive to temperature and could involve Hg interaction with membrane thiols, possibly related to solute transport.
During metal uptake from metal mixtures, Hg may thus promote the uptake of other toxic metals by increasing cell volume and
consequently cell capacity.
Deceased 25 March 2004 相似文献
6.
Kenji Kuwayama Hiroyuki Inoue Kenji Tsujikawa Yuko Iwata Naoki Kamo 《生物化学与生物物理学报:生物膜》2008,1778(1):42-50
3,4-Methylenedioxymethamphetamine (MDMA) is an illegal amphetamine-type stimulant (ATS) that is abused orally in the form of tablets for recreational purposes. The aim of this work is to investigate the absorption mechanism of MDMA and other related compounds that often occur together in ATS tablets, and to determine whether such tablet components interact with each other in intestinal absorption. The characteristics of MDMA uptake by the human intestinal epithelial Caco-2 cell line were investigated. The Michaelis constant and the maximal uptake velocity at pH 6.0 were 1.11 mM and 13.79 nmol/min/mg protein, respectively, and the transport was electroneutral. The initial uptake rate was regulated by both intra- and extracellular pH. MDMA permeation from the apical to the basolateral side was inferior to that in the reverse direction, and a decrease in apical pH enhanced MDMA permeation from the basolateral to the apical side. These facts indicate that this transport system may be an antiporter of H+. However, under physiological conditions, the proton gradient cannot drive the MDMA uptake because it is inwardly directed. Large concentration differences of MDMA itself drive this antiporter. Various compounds with similar amine moieties inhibited the uptake, but substrates of organic cation transporters (OCT1-3) and an H+-coupled efflux antiporter, MATE, were not recognized. 相似文献
7.
D. T. Thwaites G. T. A. McEwan C. D. A. Brown B. H. Hirst N. L. Simmons 《The Journal of membrane biology》1994,140(2):143-151
In human Caco-2 intestinal epithelial layers, xxxl-alanine absorption can be energized by a proton gradient across the brush-border membrane. Acidification of the apical medium, even in Na+-free media, is associated with a saturable net transepithelial absorption of xxxl-alanine. xxxl-Alanine transport causes cytosolic acidification consistent with proton/amino acid symport. xxxl-Alanine transport in Na+-free media is rheogenic, stimulating an inward short-circuit current in voltageclamped epithelial monolayers. By measurement of rapid xxxl-alanine influx across the apical membrane, xxxl-alanine-stimulated inward short-circuit current and intracellular acidification in the same cell batch, we estimate xxxl-alanine/proton stoichiometry to be 10.62 ±0.25 (xxxsd) (short-circuit current) or 10.73 ±0.19 (intracellular acidification). From competition studies, it is likely that xxxl-proline, -aminoisobutyric acid, and -alanine, but not xxxl-valine and xxxl-serine, are substrates for protonlinked, substrate transport in the brush border of Caco-2 cells.This study was supported by the Wellcome Trust (to D.T.T. and N.L.S.) and the LINK Programme in Selective Drug Delivery and Targeting (funded by the SERC/MRC/DTI and Industry). Charlotte Ward gave excellent technical assistance. 相似文献
8.
Cadmium represents a major environmental pollutant that may induce severe damage, especially in the kidney where cadmium accumulates.
While cadmium is known to severely impair renal tubular functions, glomerular structures are also potential targets. Owing
to their contractile properties, glomerular mesangial cells play a major role in the control of glomerular hemodynamics and
influence the ultrafiltration coefficient. Cell cultures provide alternative and fruitful models for study of in vitro toxicology. However, the use of primary human mesangial cell cultures is hampered by their limited survival span and their
rapid dedifferentiation during passages. This study presents a human stable immortalized mesangial cell line, designated IP15.
Cell characteristics were investigated by the detection of known mesangial markers, as well as their ability to contract in
response to angiotensin II. IP15 cells were used to investigate cadmium uptake and morphological changes such as cell contraction
and cytoskeleton protein expression. The IC50 cytotoxicity index was obtained with 3.55 μmol/L using neutral red assay for 24 h. After cadmium exposure (1 μmol/L, determined
as nonlethal concentration), 0.38 μg Cd/mg protein was internalized by the cells as evaluated by inductively coupled plasma
optical emission spectrometry (ICP/OES). Cadmium induced a significant cell surface reduction that correlated with smooth-muscle
α-actin disorganization. Thus, the IP15 cell line is a suitable model for study of in vitro cadmium cytotoxicity in mesangial cells and allows sufficient material to be obtained for future studies of the intracellular
effects of cadmium exposure. 相似文献
9.
Snowden L Wernbacher L Stenzel D Tucker J McKay D O'Brien M Katouli M 《Journal of applied microbiology》2006,101(4):964-975
AIMS: To investigate the prevalence of Aeromonas in a major waterway in South East Queensland, Australia, and their interactions with a gut epithelial model using Caco-2 cells. METHODS AND RESULTS: A total of 81 Aeromonas isolates, collected from a major waterway in South East Queensland, Australia, were typed using a metabolic fingerprinting method, and tested for their adhesion to HEp-2 and Caco-2 cells and for cytotoxin production on Vero cells and Caco-2 cells. Aeromonas hydrophila had the highest (43%) and Aeromonas veronii biovar sobria had the lowest (25%) prevalence. Four patterns of adhesion were observed on both HEp-2 and Caco-2 cell lines. Representative isolates having different phenopathotypes (nine strains) together with two clinical isolates were tested for their translocation ability and for the presence of virulence genes associated with pathogenic Escherichia coli. The rate and degree of translocation across Caco-2 monolayers varied among strains and was more pronounced with LogA pattern. Translocation was associated with the adherence of strains to Caco-2 cells microvilli, followed by internalization into Caco-2 cells. Two Aer. veronii biovar sobria strains were positive for the presence of heat-labile toxin genes, with one strain also positive for Shiga-like toxin gene. CONCLUSIONS: Pathogenic strains of Aeromonas carrying one or more virulence characteristics are highly prevalent in the waterways studied and are capable of translocating across a human enterocyte cell model. SIGNIFICANCE AND IMPACT OF THE STUDY: This study indicates that Aeromonas strains carrying one or more virulence properties are prevalent in local waterways and are capable of translocating in a human enterocyte cell culture model. However, their importance in human gastrointestinal disease has yet to be verified under competitive conditions of the gut. 相似文献
10.
We previously developed an in vitro model to estimate the relative bioavailability of carotenoids from a meal prepared using commercial baby foods. The general applicability of this model was tested using a stir-fried meal consisting of fresh spinach, fresh carrots, tomato paste, and vegetable oil. After in vitro digestion of the cooked meal, the aqueous fraction was separated from residual oil droplet and solids by centrifugation to quantify micellarized carotenoids. The percentages of lutein, lycopene, α-carotene, and β-carotene transferred from the meal to the micellar fraction were 29.0 ± 0.6, 3.2 ± 0.1, 14.7 ± 0.3, and 16.0 ± 0.4, respectively. Carotenoid transfer from the meal to the aqueous fraction was inhibited when bile extract was omitted from the intestinal phase of digestion. The bioavailability of the micellarized carotenoids was validated using differentiated cultures of Caco-2 human intestinal cells. All four carotenoids were accumulated in a linear manner throughout a 6-hr incubation period. Metabolic integrity was not compromised by exposure of cultures to the diluted aqueous fraction from the digested meal. The addition of 500 μmol/L α-tocopherol to test medium significantly improved the stability of the micellar carotenoids within the tissue culture environment. These results support the utility of the in vitro digestion procedure for estimating the bioavailability of carotenoids from foods and meals. 相似文献
11.
【目的】利用CRISPR/Cas9系统构建稳定敲除anxa6基因的Caco-2细胞株,为研究大肠杆菌O157:H7效应蛋白Esp F与宿主膜联蛋白A6 (ANXA6)相互作用及其致病机制奠定基础。【方法】根据CRISPR/Cas9靶向原理设计并合成3个特异性识别anxa6基因的向导RNA (single guide RNA,sgRNA),基于Lenti CRISPRv2载体构建Lenti CRISPRv2-sg RNA重组质粒,转入293T细胞中,制备sgRNA-Cas9慢病毒,将慢病毒感染Caco-2细胞,经嘌呤霉素筛选阳性细胞,有限稀释法分离培养单克隆细胞,提取单克隆细胞基因组DNA,并对敲除位点附近的DNA片段进行PCR扩增,测序并进行脱靶效应评估;免疫印记法检测ANXA6蛋白表达情况,细胞计数试剂盒8 (cell counting kit 8,CCK8)试剂盒检测细胞增殖能力,免疫荧光法检测细胞紧密连接分布情况。【结果】Western blotting及序列测序表明anxa6基因敲除单克隆细胞构建成功;脱靶效应评估结果显示预测的10个脱靶位点均无脱靶现象;基因敲除对细胞增殖能力... 相似文献
12.
《Journal of trace elements in medicine and biology》2014,28(3):344-350
Copper sulfate (CuSO4), micron copper oxide (micron CuO) and nano copper oxide (nano CuO) at different concentrations were, respectively, added to culture media containing Caco-2 cells and their effects on Ctr1, ATP7A/7B, MT and DMT1 gene expression and protein expression were investigated and compared. The results showed that nano CuO promoted mRNA expression of Ctr1 in Caco-2 cells, and the difference was significant compared with micron CuO and CuSO4. Nano CuO was more effective in promoting the expression of Ctr1 protein than CuSO4 and micron CuO at the same concentration. Nano CuO at a concentration of 62.5 μM increased the mRNA expression levels of ATP7A and ATP7B, and the difference was significant compared with CuSO4. The addition of CuSO4 and nano CuO to the culture media promoted the expression of ATP7B proteins. CuSO4 at a concentration of 125 μM increased the mRNA expression level of MT in Caco-2 cells, and the difference was significant compared with nano CuO and micron CuO. Nano CuO at a concentration of 62.5 μM inhibited the mRNA expression of DMT1, and the difference was significant compared with CuSO4 and micron CuO. Thus, the effects of CuSO4, micron CuO and nano CuO on the expression of copper transport proteins and the genes encoding these proteins differed considerably. Nano CuO has a different uptake and transport mechanism in Caco-2 cells to those of CuSO4 and micron CuO. 相似文献
13.
Discs of thallus cut from the macroalga Ulva lactuca were incubated in filtered seawater containing cadmium, zinc, copper or cobalt (30
m). The metal uptake rates differed for each metal in the order Cu > Zn > Cd > Co. Exposure of the macroalga to metals resulted in a disruption of intracellular monovalent cation composition. Intracellular potassium was irreversibly lost and sodium was accumulated by cadmium- or copper-treated U. lactuca, which was assumed to indicate irreversible disruption of the plasmalemma. Exposure to zinc caused an increase in sodium concentrations, whereas potassium concentrations were not significantly different from the controls, suggesting that the integrity of the plasmalemma had been maintained at the zinc concentration used. Intracellular magnesium was also lost from copper-treated algae, which again indicated a loss of integrity of the cell membrane. 相似文献
14.
Comparative strategies of heavy metal accumulation by crustaceans: zinc,copper and cadmium in a decapod,an amphipod and a barnacle 总被引:10,自引:2,他引:8
This study investigates the comparative strategies of accumulation under standardised laboratory conditions of the essential metals zinc and copper, and the non-essential metal cadmium by three crustaceans of different taxa; vizPalaemon elegans Rathke (Malacostraca: Eucarida: Decapoda),Echinogammarus pirloti (Sexton & Spooner) (Malacostraca: Peracarida: Amphipoda) and the barnacleElminius modestus Darwin (Cirripedia: Thoracica).The decapodP. elegans regulates body zinc concentrations to a constant level (ca. 79 µg Zn g–1) over a wide range of dissolved metal availabilities until regulation breaks down at high Zn availabilities and net accumulation begins. The amphipodE. pirloti accumulates zinc at all dissolved zinc concentrations but at a low net rate such that the accumulation strategy approaches that of regulation. The barnacleE. modestus accumulates zinc to high body concentrations with no significant excretion of accumulated zinc. In the case of copper,P. elegans similarly regulates body copper concentrations to a constant level (ca. 129 µg Cu g–1) over a range of dissolved copper availabilities until regulation breaks down at high copper concentrations. Both the amphipodE. pirloti and the barnacleE. modestus on the other hand accumulate copper at all dissolved copper exposures with no evidence of regulation. All three crustaceans accumulate the non-essential metal cadmium at all dissolved cadmium concentrations without regulation.Heavy metal accumulation strategies therefore vary between crustacean taxa and between metals. Uptake rates for zinc and cadmium have been estimated for the three crustaceans and can be interpreted in terms of cuticle permeability and way of life of each crustacean. Examination of these uptake rates provides an insight into possible reasons behind the adoption of particular metal accumulation strategies. 相似文献
15.
Anna Źróbek-Sokolnik Han Asard Kamilla Górska-Koplińska Ryszard J. Górecki 《Acta Physiologiae Plantarum》2009,31(1):43-49
Generation of reactive oxygen species (ROS) constitutes an important first reaction under many stress conditions in plants.
We demonstrate that Nicotiana tabacum L. cv. Bright Yellow 2 (TBY-2) cells in suspension cultures, generate superoxide radical and hydrogen peroxide upon treatment
with cadmium and zinc. Addition of catalase and N,N-diethyldithiocarbamate (DDC) decreased the level of H2O2, whereas superoxide dismutase (SOD) induced a slight increase of the H2O2 production. The effects of catalase, DDC and SOD on the heavy metal-induced ROS production indicate that it occurs outside
of the cells, and that at least part of the hydrogen peroxide is produced by dismutation of the superoxide radical (O2
·−). The effect of pretreatment of the cell cultures with commonly used mammalian NADPH oxidase inhibitors was also tested.
Strong inhibitions of cadmium and zinc-mediated ROS production were obtained with the flavoprotein inhibitors—diphenylene
iodonium (DPI) and quinacrine and with an inhibitor of b-type cytochromes—imidazol. Membrane permeable-N-ethyl maleimide (NEM) and iodoacetate, and membrane non-permeable thiol reagents—para-chloromercuribenzoic acid (pCMBS) also
inhibited the ROS production. These results suggested that the enzyme responsible for cadmium and zinc-induced ROS production
in tobacco cells contains a flavocytochrome. They also show the importance of intra- and extracellular thiol groups in the
observed stress reaction. The induction of ROS production with heavy metals showed properties comparable to the elicitor-induced
oxidative burst in other plant cells. 相似文献
16.
Transport characteristics of certain inorganic elements such as copper, magnesium, selenium and iron have been studied in maternal-fetal direction in normal pregnancies, using in vitro perfusion of isolated placental lobules. Copper, selenium, magnesium and iron salts corresponding to twice physiological concentrations were injected as a 100 l bolus, into the maternal arterial perfusate. Serial perfusate samples were collected from venous outflows for a study period of 5 min. Concentrations of various inorganic elements and their transport kinetics were determined. Transport fractions of copper, selenium, magnesium and iron averaged 0.14, 0.19, 0.06 and 0.23% of maternal load respectively. The pharmacokinetic parameters such as area under the curve, clearance, elimination constant, and time for maximum response showed some significant differences between the various elements. We speculate that copper and selenium share the same transport pathway along a concentration gradient in maternal-fetal direction, while for iron and magnesium, active transport plays a predominant role for element transfer across the human placental membrane. 相似文献
17.
Chauvin J.E. Souchet C. Dantec J.P. Ellissèche D. 《Plant Cell, Tissue and Organ Culture》2003,73(1):65-73
A potato breeding scheme implies the possibility of ploidy level manipulation either by reducing the chromosome number of cultivars from 48 to 24 to be able to cross them with diploid related species or by doubling diploid material to reach the generally optimal tetraploid level. In vitro spontaneous chromosome doubling is widely used but can lead to somaclonal variation. Since oryzalin has proven to be efficient as a chromosome doubling agent on potato cell suspension cultures, we tried this herbicide on various Solanum species and interspecific diploid hybrids. A 24 h dip in a 28.8 M aqueous oryzalin solution applied on apical buds was the most efficient treatment in terms of tetraploid plant production (mean = 4.1 tetraploid plants for 10 treated buds over 4 genotypes). However 50–100% of the regenerated tetraploid plants acclimatized after in vitro treatment proved to be chimaeric. Consequently, a selection procedure in the progeny was necessary to obtain real and stable doubled clones and final yields were low. This technique is easy to apply and could be a good alternative to chromosome doubling by spontaneous in vitro regeneration in the case of refractory genotypes especially where somaclonal variation is problematic. Percentage of tetraploids among the regenerated plants varied from 6 to 29% with the oryzalin doubling technique while it varied from 20 to 78% by in vitro spontaneous doubling for five diploid genotypes. An observation of the progeny indicated that chimaeras were more frequent using oryzalin (50–100% of the initially supposed tetraploid plants) than when chromosomes doubled spontaneously (4–67% of the initially supposed tetraploid plants). 相似文献
18.
Functional regulation of Na+-dependent neutral amino acid transporter ASCT2 by S-nitrosothiols and nitric oxide in Caco-2 cells 总被引:3,自引:0,他引:3
We describe the regulation mechanisms of the Na(+)-dependent neutral amino acid transporter ASCT2 via nitric oxide (NO) in the human intestinal cell line, Caco-2. Exposure of Caco-2 cells to S-nitrosothiol, such as S-nitroso-N-acetyl-DL-penicillamine (SNAP) and S-nitrosoglutathione, and the NO-donor, NOC12, concentration- and time-dependently increased Na(+)-dependent alanine uptake. Kinetic analyses indicated that SNAP increases the maximal velocity (V(max)) of Na(+)-dependent alanine uptake in Caco-2 cells without affecting the Michaelis-Menten constant (K(t)). The stimulatory effect was partially eliminated by actinomycin D and cycloheximide. Increased Na(+)-dependent alanine uptake by SNAP was partially abolished by the NO scavengers, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide sodium salt (carboxy-PTIO) and N-(dithiocarboxy)sarcosine disodium salts (DTCS), as well as the NADPH oxidase inhibitor, diphenyleneiodonium. RT-PCR revealed that Caco-2 cells expressed the Na(+)-dependent neutral amino acid transporter ASCT2, but not the other Na(+)-dependent neutral amino acid transporters ATB(0,+) and B(0)AT1. These results suggested that functional up-regulation of ASCT2 by SNAP might be partially associated with an increase in the density of transporter protein via de novo synthesis. 相似文献
19.
Sesbania rostrata, an annual tropical legume, has been found to be tolerant to heavy metals, with an unknown mechanism. It is a promising candidate
species for revegetation at mine tailings. In this study, sequential extractions with five buffers and strong acids were used
to extract various chemical forms of cadmium and copper in S. rostrata, with or without Cd or Cu treatments, so that the mechanisms of tolerance and detoxification could be inferred. Both metals
had low transition rates from roots to the aboveground of S. rostrata. The transition ratio of Cd (4.00%) was higher than that of Cu (1.46%). The proportion of NaCl extracted Cd (mostly in protein-binding
forms) increased drastically in Cd treated plants from being undetectable in untreated plants. This suggests that Cd induced
biochemical processes producing protein-like phytochelatins that served as a major mechanism for the high Cd tolerance of
S. rostrata. The case for Cu was quite different, indicating that the mechanism for metal tolerance in S. rostrata is metal-specific. The proportion of water-insoluble Cu (e.g. oxalate and phosphate) in roots increased significantly with
Cu treatment, which partially explains the tolerance of S. rostrata to Cu. However, how S. rostrata copes with the high biotic activity of inorganic salts of Cu, which increased in all parts of the plant under Cu stress,
is a question for future studies. Sesbania rostrata is among the very few N-fixing plants tolerant to heavy metals. This study provides evidence for the detoxification mechanism
of metals in Sesbania rostrata. 相似文献
20.
Yuko Inamochi Kazuki Mochizuki Toshinao Goda 《Biochimica et Biophysica Acta (BBA)/General Subjects》2014