NITROGEN METABOLISM IN WHITE POTATO TUBER TISSUE INFECTED WITH FUNGI. CHANGES IN THE ALCOHOL-SOLUBLE AND -INSOLUBLE NITROGEN FRACTIONS

An increase of the ratio insoluble N/soluble N in potato tubertissue (var. Bintje) infected with Gibberella saubinetii orCerato-cystis fimbriata and incubated at 25° could onlybe demonstrated in samples cut from the area invaded by thefungi. In the non-infected controls some increase was foundin tissue close to the cut surface due to wound-reaction. The fungi hardly penetrated tuber tissue of var. Koopmans Blauwe,while tubers of var. Alpha were rather susceptible. Tissue ofvar. Bintje turned out to be rather resistant. Incubation at30° or 33° resulted in a more limited penetration bythe fungi. No relation exists between the value of the ratioinsoluble N/soluble N or the protein content of the host tissue,and the grade of resistance to the infection. Again, an increaseof insoluble N could only be observed in mycelium-containingsamples. The mycelia of both fungi had a ratio insoluble N/soluble Nwhich was much higher than the ratio of normal potato tissue.It was possible to explain the increase of the ratio in invadedtissue on the basis of the presence of hyphal substances. No evidence was obtained for a synthesis by the host tissueof insoluble nitrogenous compounds prior to, or concomitantwith, the increase of respiration rate after infection. To whatextent a wound-reaction played a role in the infected tuberparts is uncertain. The possibility of a simultaneous synthesisand breakdown of proteins after infection without a rise ininsoluble nitrogen is discussed. (Received February 3, 1966; )     

LIQUID NITROGEN IN THE TREATMENT OF SKIN DISEASES

Liquid nitrogen is a satisfactory freezing agent in the treatment of such skin diseases as warts, keratoses, superficial hemangiomas, leukoplakia, keloids, superficial scarring and dermatitis venenata. It is available and easily applied. Its use is described and the results of treatment in 154 cases of warts are presented.     

METABOLIC CHANGES IN THE BRAINS OF MICE FROZEN IN LIQUID NITROGEN

Abstract— Autolytic changes in the mouse brain, occurring during immersion of the animal in liquid nitrogen, were evaluated by measuring the tissue concentrations of glucose, lactate, pyruvate, α-oxoglutarate, phosphocreatine, creatine, ATP, ADP and AMP. The values thus obtained were compared with those obtained in paralysed mice under nitrous oxide anaesthesia, the brains of which were frozen in such a way that arterial blood pressure and oxygénation were upheld during the freezing. Immersion of unanaesthetized mice in liquid nitrogen gave rise to significant alterations in phosphocreatine, creatine, lactate, lactate/pyruvate ratio, ADP and AMP. A comparison with values obtained in paralysed and anaesthetized mice that were frozen by immersion in liquid nitrogen showed that the metabolic changes observed in the unanaesthetized animals could not be caused by an anaesthetic effect on the metabolic pattern. It is concluded that autolysis in the mouse brain occurs during immersion of the animal in a coolant, mainly because arterial hypoxia develops before the tissue is frozen. A comparison with previous results on rat cerebral cortex indicates that mice offer no advantage for studies of cerebral metabolites in unanaesthetized animals. In both species, accurate analyses of labile cerebral metabolites require that the brain is frozen in a way that prevents arterial hypoxia during the fixation of the tissue.     

稻田土壤－作物－家畜系统中氮的循环研究

本文用Ｎ￣（１５）标记水稻和绿肥研究了稻田土壤－作物－家畜系统中氮的循环。Ｎ￣（１５）标记稻草喂羊,羊体回收饲料稻草Ｎ３１．１６％,羊粪２８．２６％,羊尿５．７２％,总回收６５．１４％,损失３４．８６％。将羊粪尿单施,稻谷回收饲料稻草Ｎ３．１９％,水稻全株回收４．８２％,土壤残留１９．００％,损失１０．１４％。故羊体、水稻及土壤残留共回收饲料稻草Ｎ５４．９８％．将羊粪与尿素配施,则饲料稻草Ｎ的总回收率为５５．８８％。Ｎ￣（１５）标记绿肥喂猪;猪体回收饲料绿肥Ｎ２３．５１％．猪粪２３．８５％,猪尿２８．７６％,总回收率７６．１２％,损失率２３．８８％。将猪粪、尿还田,稻谷回收饲料绿肥Ｎ６．６９％,水稻全株回收１０．０５％,土壤残留１９．１７％,故猪体、水稻和土壤残留共回收饲料绿肥Ｎ５２．７３％,将猪粪与尿素配施,则饲料绿肥Ｎ的总回收率为５２．７５％。     

稻田土壤－作物－家畜系统中氮的循环研究

本文用Ｎ＾１５标记水稻和绿肥研究了稻田土壤－作物－家畜系统中氮的循环。Ｎ＾１５标记稻草喂羊,羊体回收饲料稻草Ｎ３１．１６％,羊粪２８．２６％,羊尿５．７２％,总回收６５．１４％,损失３４．８６％。将羊粪尿单施,稻谷回收饲料稻草Ｎ３．１９％,水稻全株回收４．８２％,水稻全株回收４．８２％,土壤残留１９．００％,损失１０．１４％。故羊体,水稻及土壤残留共回收饲料稻草Ｎ５４．９８％。将羊粪与尿素配施,则     

THE RELATION BETWEEN BIOCHEMICAL AND MORPHOLOGICAL DIFFERENTIATION IN BLASTOCLADIELLA EMERSONII. II. NITROGEN METABOLISM IN SYNCHRONOUS CULTURES

Lovett, James S., and Edward C. Cantino. (Michigan State University, East Lansing, Michigan.) The relation between biochemical and morphological differentiation in Blastocladiella emersonii. II. Nitrogen metabolism in synchronous cultures. Amer. Jour. Bot. 47(7): 550–560. Illus, 1960.—A procedure was developed for growing well-synchronized, large-scale cultures of resistant-sporangial plants of Blastocladiella throughout one complete generation period in a glucose-peptone-yeast medium containing NaHCO₃. The increase in size and dry weight of individual plants and a photomicrographic record of their developmental morphology were obtained during the growth of such cultures. The activity of glucosamine synthetase, glucose-6-phosphate dehydrogenase, and phosphoglucoisomerase was determined during development in synchronous culture. The sequence for synthesis of chitin, lipid, melanin, and nitrogenous materials was also established. The free-N pools in developing resistant sporangial plants underwent both quantitative and qualitative changes during differentiation. The significance of the alteration in cellular components and their relationship to the structure and function of the developing thallus were discussed.