Pathological and ecological host consequences of infection by an introduced fish parasite

The infection consequences of the introduced cestode fish parasite Bothriocephalus acheilognathi were studied in a cohort of wild, young-of-the-year common carp Cyprinus carpio that lacked co-evolution with the parasite. Within the cohort, parasite prevalence was 42% and parasite burdens were up to 12% body weight. Pathological changes within the intestinal tract of parasitized carp included distension of the gut wall, epithelial compression and degeneration, pressure necrosis and varied inflammatory changes. These were most pronounced in regions containing the largest proportion of mature proglottids. Although the body lengths of parasitized and non-parasitized fish were not significantly different, parasitized fish were of lower body condition and reduced weight compared to non-parasitized conspecifics. Stable isotope analysis (δ(15)N and δ(13)C) revealed trophic impacts associated with infection, particularly for δ(15)N where values for parasitized fish were significantly reduced as their parasite burden increased. In a controlled aquarium environment where the fish were fed ad libitum on an identical food source, there was no significant difference in values of δ(15)N and δ(13)C between parasitized and non-parasitized fish. The growth consequences remained, however, with parasitized fish growing significantly slower than non-parasitized fish, with their feeding rate (items s(-1)) also significantly lower. Thus, infection by an introduced parasite had multiple pathological, ecological and trophic impacts on a host with no experience of the parasite.     

Comparisons of the food niches of three native and two introduced fish species in an Australian river

Synopsis The breadth, correspondence and overlap of the diets of the small and large size classes of the three native species (Galaxias occidentalis, Bostockia porosa and Edelia vittata) and two introduced species (Gambusia holbrooki and Perca fluviatilis) of fishes found in the shallows of the main channel and in the tributary creeks of a south-western Australian river have been compared in each season. Classification and ordination were used to examine the overall interrelationships of the diets across species, size groups, seasons and the location where the fish were caught (channel or creek). The smaller fish had a narrower dietary breadth than larger fish in the spring and summer, presumably reflecting the size limit imposed on prey size by their possession of a relatively small mouth in these seasons. Intraspecific dietary overlap between large and small size classes was usually high in G. occidentalis, but generally low in G. holbrooki and P. fluviatilis, and also in B. porosa and E. vittata when the difference between the lengths of the two size groups was greatest. Dietary overlap was least in autumn when the main prey taxa were most abundant. During winter, the diets of the three native species in the tributaries converged, probably reflecting a relatively low faunal diversity in these highly seasonal water bodies. The only relatively consistent interspecific overlap in diet was between B. porosa and E. vittata. Classification and ordination of the dietary samples separated G. occidentalis (which fed extensively on terrestrial organisms from the water surface) from the smaller P. fluviatilis (that concentrated on copepods in the plankton) and from B. porosa and E. vittata (which ingested primarily benthic organisms). Furthermore, B. porosa tended to ingest larger prey taxa than E. vittata. The diet of Gambusia holbrooki is sometimes dominated by terrestrial insects and at other times by benthic organisms, demonstrating that this species is an opportunistic carnivore. It is concluded that food partitioning by the three native and two introduced fish species in the Collie River is likely to be one of the principal factors facilitating the coexistence of substantial populations of these species in this system.     

Similarities in correlates of native and introduced fish species richness distribution in Brazilian reservoirs

We assessed the relationships among native and introduced fish species richness and a set of explanatory variables, including area, altitude, reservoir age, temperature, human influence index and fish abundance. We expected to find different relationships based on species origin (i.e., native or introduced). Based on compiled data from Brazilian reservoirs, we modeled the variation in the number of native and introduced fish species by generalized linear mixed models with different spatial autocorrelation structures. Reservoirs located in warmer regions and with higher fish abundance showed higher species richness of both native and introduced fish. Reservoir age tended to be negatively correlated with native species richness. Our results suggest that reservoir communities in warmer regions may be more resistant to local extinctions caused by impoundments, but they are more susceptible to species introductions. Our results also highlight that an extinction debt can be expected for native species because older reservoirs showed lower native species richness.     

Conservation ofHypericum foliosum aiton, an endemic azorean species, by micropropagation

Summary One of the first Azorean endemic vascular taxa chosen for the development ofin vitro multiplication techniques wasHypericum foliosum Aiton, due to its colonizing ability (Sj?gren, 1984), a loss of seed germination capacity after only 1 yr of storage (Maciel, 1994), and the populations' generally low number of individuals. The following culture media were tested usingHypericum foliosum's single node cuttings: Murashige and Skoog (1962), Roest and Bockelmman (1973), Lloyd and McCown (1980), C?rte and Mendon?a (1985), and Cellárová et al. (1992). Further experiments were performed on CM medium supplemented with four different growth regulators: α-naphthaleneacetic acid (NAA), N⁶-benzyladenine (BA), γ, γ-(dimethylallyl) aminopurine (2iP), and kinetin (KIN). The acclimatization stage was carried out in Jiffy 7? pots and in a 2∶1 or 1∶1 peat/perlite mixture. We found that micropropagation ofHypericum foliosum is possible on CM medium and that the best results with growth regulators were achieved with the following supplements: 0.1 mg/l (0.4 μM) BA and 0.5 mg/l (2.6 μM) NAA+1.0 mg/l (4.4 μM) BA (in the initiation stage), and 0.1 mg/l (0.4 μM) BA (in the elongation stage). As for culture multiplication, 0.1 mg/l (0.4 μM) BA (in the initiation stage) and 0.5 mg/l (2.6 μM) NAA+1.0 mg/l (4.4 μM) BA (both in the initiation and elongation stages), proved to be the most efficient concentrations. The acclimatization stage was successfully performed in Jiffy 7? pellets.     

Amplification of autoimmune disease by infection

Reports of infection with certain chronic persistent microbes (herpesviruses or Chlamydiae) in human autoimmune diseases are consistent with the hypothesis that these microbes are reactivated in the setting of immunodeficiency and often target the site of autoimmune inflammation. New experimental animal models demonstrate the principle. A herpesvirus or Chlamydia species can be used to infect mice with induced transient autoimmune diseases. This results in increased disease severity and even relapse. The evidence suggests that the organisms are specifically imported to the inflammatory sites and cause further tissue destruction, especially when the host is immunosuppressed. We review the evidence for the amplification of autoimmune inflammatory disease by microbial infection, which may be a general mechanism applicable to many human diseases. We suggest that patients with autoimmune disorders receiving immunosuppressing drugs should benefit from preventive antiviral therapy.     

Drought, differential mortality and the coexistence of a native and an introduced fish species in a south east Australian intermittent stream

Synopsis The longitudinal distribution ofSalmo trutta andGalaxias olidus, a small salmoniform fish, was mapped over four summers (1985–1988) in the upper reaches of the Lerderderg River, an intermittent stream in central Victoria. Over the four successive summers of the study, the distribution ofS. trutta expanded upstream. Coincident with the expansion ofS. trutta was a contraction in the distribution ofG. olidus upstream. In the summer of 1988, an extended dry spell resulted in high levels ofS. trutta mortality at the upstream limits of their distribution. This suggested that the upstream distribution ofS. trutta could ultimately be constrained by an inability to tolerate prolonged periods of low stream flow and high temperatures that tended to occur in the headwaters of the stream. A study of summer mortality was conducted over three successive summers (1989–1991) at three reaches along the river. The two downstream reaches contained onlyS. trutta, the upstream reach contained bothS. trutta andG. olidus. Salmo trutta mortality was consistently higher at the most upstream reach compared with lower reaches. High levels ofS. trutta mortality at the two downstream reaches were only observed in 1991, a particularly dry year. In contrast,G. olidus survived in scattered small pools throughout the upper reaches of the stream. The results of the study suggest a shifting pattern ofS. trutta andG. olidus distribution determined by the duration of low stream flow periods and summer high temperatures.     

Tinea capitis an endemic disease in Madras

A study ofTinea capitis in Outpatient Clinic, Skin Department, Government General Hospital, Madras during a three year period from November, 1973 to October, 1976, has shown a gradual increase in incidence of 3.56%, 5.09 % and 6.25 % respectively. Findings suggest thatTinea capitis is endemic in South India. Male children were more commonly affected than female children and the age groups chiefly affected were between 5 and 10 years. A considerable number of adults were also affected. The disease showed no correlation to environmental temperature, humidity and rainfall but was correlated to all types of mycoses and total incidence of mycoses.Among 357 isolates,Trichophyton violaceum was the commonest in 264 (73.94%) andT. tonsurans was the next common in 47 (13.16%). The other agents wereT. rubrum in 30 (8.4%),T. mentagrophytes in 11 (3.08%) andT. simii in 5 (1.4%). Noninflammatory lesions were more common than inflammatory lesions and both were produced byT. violaceum andT. tonsurans, suggesting strain differences in pathogenesis. Treatment with oral griseofulvin was satisfactory in all but had to be discontinued in 4 patients due to side effects.     

Not an ancient relic: the endemic Livistona palms of arid central Australia could have been introduced by humans

Livistona mariae is an endemic palm localized in arid central Australia. This species is separated by about 1000 km from its congener L. rigida, which grows distantly in the Roper River and Nicholson-Gregory River catchments in northern Australia. Such an isolated distribution of L. mariae has been assumed to have resulted from contraction of ancestral populations as Australia aridified from the Mid-Miocene (ca 15 Ma). To test this hypothesis at the population level, we examined the genetic relationships among 14 populations of L. mariae and L. rigida using eight nuclear microsatellite loci. Our population tree and Bayesian clustering revealed that these populations comprised two genetically distinct groups that did not correspond to the current classification at species rank, and L. mariae showed closest affinity with L. rigida from Roper River. Furthermore, coalescent divergence-time estimations suggested that the disjunction between the northern populations (within L. rigida) could have originated by intermittent colonization along an ancient river that has been drowned repeatedly by marine transgression. During that time, L. mariae populations could have been established by opportunistic immigrants from Roper River about 15 000 years ago, concurrently with the settlement of indigenous Australians in central Australia, who are thus plausible vectors. Thus, our results rule out the ancient relic hypothesis for the origin of L. mariae.     

The distribution of an invasive fish species is highly affected by the presence of native fish species: evidence based on species distribution modelling

Topmouth gudgeon (Pseudorasbora parva) is one of the most invasive aquatic fish species in Europe and causes adverse effects to ecosystem structure and functioning. Knowledge and understanding of the species’ interactions with the environment and with native fish are important to stop and prevent the further spread of the species. Creating species distribution models is a useful technique to determine which factors influence the occurrence and abundance of a species. We applied three different modelling techniques: general additive models, random forests and fuzzy habitat suitability modelling (FHSM) to assess the habitat suitability of topmouth gudgeon. The former two techniques indicated that the abundance of native fish (i.e. biotic variables) was more important than environmental variables when determining the abundance of topmouth gudgeon in Flanders (Belgium). Bitterling (Rhodeus amarus), stone loach (Barbatula barbatula), three-spined stickleback (Gasterosteus aculeatus) and predator abundance were selected as the most important biotic variables and implemented in the FHSM to investigate species interactions. Depending on the preferred food source and spawning behaviour, either coexistence or interspecific competition can occur with bitterling, stone loach and three-spined stickleback. In contrast, the presence of predators clearly had a top down effect on topmouth gudgeon abundance. These findings could be applied as a biological control measure and implemented in conservation strategies in order to reduce the abundance of earlier established populations of topmouth gudgeon.     

Trophic ecology of native and introduced fish species from the Chillán River, South-Central Chile

The native continental ichthyofauna of Chile has a low richness in species and marked endemism in comparison to the rest of South America. Since the end of the 19th century 26 fish species have been introduced into the Chilean systems. The study covers the trophic ecology of three native and two exotic fish species from the Chillán River. Stomach contents were analyzed and prey items identified at the family level, then compared with the environmental availability by sampling the benthos and using Ivlev's Electivity Index to determine the degree of election. The relative importance of each prey item was determined and the Morisita Index applied to determine the level of overlap. The results indicate a greater trophic spectrum for introduced rather than for native species. The Relative Importance Index identified Chironomidae as the most important prey item for all species studied. An elevated overlap was observed between the diets of the five species, being greater within the native and introduced species (> 0.9) than between the two groups (< 0.8). These results do not reveal competition, but allow the authors to infer the negative effects of the introduced species to the conservation of native fish.     

Competition for food between an introduced crayfish and two fishes endemic to the Colorado River basin

Synopsis Crayfish are not native to the Colorado River basin (CRB), however they are now established in portions of the mainstem and in many tributaries. I used density manipulation experiments in a laboratory setting to determine intra- and interspecific competition for food between Orconectes virilis, an aggressive polytrophic crayfish now common in the CRB, and two native fishes: Gila chub, Gila intermedia, and flannelmouth sucker, Catostomus latipinnis. I tested each fish species in separate trials. Growth of Gila chub decreased when animal densities increased, however they were more affected by intraspecific competition than by crayfish presence. In contrast, growth of flannelmouth suckers was more affected by crayfish than by intraspecific competition. Crayfish growth was not significantly altered by presence of either fish. Crayfish thus reduced fish growth by competition for food, but the effect differed markedly between the two species. An erratum to this article can be found at .     

The invasiveness of an introduced species does not predict its impact

Inconsistent use of terminology plagues the study and management of biological invasions. The term “invasive” has been used to describe inter alia (1) any introduced non-indigenous species; (2) introduced species that spread rapidly in a new region; and (3) introduced species that have harmful environmental impacts, particularly on native species. The second definition in various forms is more commonly used by ecologists, while the third definition is pervasive in policy papers and legislation. We tested the relationship between the invasiveness of an introduced species and its impact on native biodiversity. We quantified a species’ invasiveness by both its rate of establishment and its rate of spread, while its impact was assigned a categorical ranking based on the documented effects of the invader on native species populations. We found no correlations between these variables for introduced plants, mammals, fishes, invertebrates, amphibians and reptiles, suggesting that the mechanisms of invasion and impact are not strongly linked. Our results support the view that the term “invasive” should not be used to connote negative environmental impact.     

Trichomonad infection in endemic and introduced columbids in the Seychelles

Island endemic avifaunas face many threats, including the now well-documented impacts of pathogens. The impacts of pathogens on the endemic Seychelles avifauna, however, have been little studied. The protozoan parasite Trichomonas gallinae has been shown to reduce survival and reproductive success of the endemic Pink Pigeon Columba mayeri on the nearby island of Mauritius. I investigated trichomonad infection prevalence and pathogenicity in endemic Seychelles Blue Pigeons, Alectroenas pulcherrima, and two introduced species of columbid, the Madagascar Turtle-dove, Streptopelia picturata, and the Barred Ground Dove, Geopelia striata, on the Seychelles island of Mahé during September-October 2007. I asked whether: 1) trichomonad infections occur in these species; 2) prevalence varies among species; and 3) birds show any signs of pathogenicity consistent with tricho-monosis. I use the results to assess the potential threat of this pathogen to A. pulcherrima. All three species were infected with trichomonads, and the overall prevalence was 27.5%. Alectroenas pulcherrima had higher prevalence (47.1%) than the two introduced species combined (24.3%). No infected individuals showed any signs of disease. These findings suggest that trichomonad parasites should be considered as a potential disease threat to the A. pulcherrima population.     

Invasiveness of an introduced species: the role of hybridization and ecological constraints

Introduced species are confronted with new environments to which they need to adapt. However, the ecological success of an introduced species is generally difficult to predict, especially when hybridizations may be involved in the invasion success. In western Europe, the lake frog Pelophylax ridibundus appears to be particularly successful. A reason for this species’ success might be the presence of the invader’s genetic material prior to the introduction in the form of a hybrid between P. ridibundus and a second indigenous water frog species. These hybrids reproduce by hybridogenesis, only transmitting the ridibundus genome to gametes and backcrossing with the indigenous species (i.e. P. lessonae). This reproductive system allows the hybrid to be independent from P. ridibundus, and allows the ridibundus genome to be more widely spread than the species itself. Matings among hybrids produce newly formed P. ridibundus offspring (N), if the genomes are compatible. Therefore, we hypothesize that hybridogenesis increases the invasiveness of P. ridibundus (1) by enhancing propagule pressure through N individuals, and/or (2) by increasing adaptation of invaders to the native water frogs’ habitat through hybrid-derived ridibundus genomes that are locally adapted. We find support for the first hypothesis because a notable fraction of N tadpoles is viable. However, in our semi-natural experiments they did not outperform ridibundus tadpoles in the native water frogs’ habitat, nor did they differ physiologically. This does not support the second hypothesis and highlights ecological constraints on the invasion. However, we cannot rule out that these constraints may fall with ongoing selection, making a replacement of indigenous species highly probable in the future.     

Interactions between an introduced and indigenous coccinellid species at different prey densities

Coccinella septempunctata L. (Coleoptera: Coccinellidae), a Palearctic coccinellid, has established and rapidly spread throughout the United States. This quantitative examination of larval interactions between C. septempunctata and Coleomegilla maculata (DeGeer) (Coleoptera: Coccinellidae), a Nearctic coccinellid, was conducted under controlled prey densities. Larvae of both coccinellid species are affected by a limited diet [one pea aphid per day Acyrthosiphon pisum (Harris) (Homoptera: Aphididae)] compared with an excess diet (>20 aphids per day). Larval survival decreased from 86 to 63% in C. maculata and from 84 to 33% in C. septempunctata; mean preimaginal developmental time increased from 20.6 to 26.7 days in C. maculata, and from 18.1 to 32.0 days in C. septempunctata. Additionally, on one aphid per day, mean adult weight was reduced from 12.39 to 9.79 mg in C. maculata, and from 39.57 to 14.44 mg in C. septempunctata. Interspecific interactions, favoring C. septempunctata over C. maculata at a␣low prey density (one aphid per day), take the form of␣reduced survival of C. maculata compared with C.␣septempunctata (14 versus 66%). Reduced survival of␣C. maculata may be the result of competition for aphids or intraguild predation by C. septempunctata on C.␣maculata. No interspecific interactions (measured as effects on larval survival, preimaginal developmental time, and adult weight) were observed between larvae of these two species at a high prey density (>20 aphids per predator per day). Received: 15 September 1997 / Accepted: 20 May 1998     

Habitat use by endemic and introduced rodents along a gradient of forest disturbance in Madagascar

We used logistic and Poisson regression models to determine factors of forest and landscape structure that influence the presence and abundance of rodent species in the rain forest of Ranomafana National Park in southeastern Madagascar. Rodents were collected using live-traps along a gradient of human disturbance. All five endemic rodent species (Nesomys rufus, N. audeberti, Eliurus tanala, E. minor and E. webbi) and the introduced rat Rattus rattus were captured in both secondary and primary forests, but the introduced Mus musculus was only trapped in secondary forest. The abundance of R. rattus increased with the level of habitat disturbance, and it was most common in the heavily logged secondary forest. Furthermore, the probability of the presence of R. rattus increased with decreasing distance from forest edge and decreasing canopy cover, while the probability of presence increased with increasing herbaceous cover, altitude and overstory tree height. The species was never observed farther than 500 m away from human habitation or camp-site. N. rufus prefered selectively-logged forest at altitudes above 900 m a.s.l. Its probability of presence increased with increasing canopy cover, herbaceous cover and distance from forest edge, and with decreasing density of fallen logs, overstory tree height and distance from human habitation. N. audeberti prefered heavily-logged areas, while E. tanala was the only species occurring along the entire range of forest disturbance. We suggest that in the Ranomafana National Park the spread of R. rattus is associated with deforestation.     

Murine viruses in an island population of introduced house mice and endemic short-tailed mice in Western Australia

House mice (Mus domesticus) were recently introduced to Thevenard Island, off the northwest coast of Western Australia. This island is also habitat for an endangered native rodent, the short-tailed mouse (Leggadina lakedownensis). Concerns have been raised that house mice may pose a threat to L. lakedownensis both through competition and as a source of infection. To assess the threat to L. lakedownensis posed by viral pathogens from M. domesticus, a serological survey was conducted from 1994 to 1996 of both species for evidence of infection with 14 common murine viruses (mouse hepatitis virus, murine cytomegalovirus, lymphocytic choriomeningitis virus, ectromelia virus, mouse adenovirus strains FL and K87, minute virus of mice, mouse parvovirus, reovirus type 3, Sendai virus, Theiler's mouse encephalomyelitis virus, polyoma virus, pneumonia virus of mice, and encephalomyocarditis virus) and Mycoplasma pulmonis. Despite previous evidence that populations of free-living M. domesticus from various locations on the Australian mainland were infected with up to eight viruses, M. domesticus on Thevenard Island were seropositive only to murine cytomegalovirus (MCMV). Antibodies to MCMV were detected in this species at all times of sampling, although seroprevalence varied. Infectious MCMV could be isolated in culture of salivary gland homogenates from seropositive mice. In contrast, L. lakedownensis on Thevenard Island showed no serological evidence of infection with MCMV, any of the other murine viruses, or M. Pulmonis, and no virus could be isolated in culture from salivary gland homogenates. Although MCMV replicated to high titers in experimentally infected inbred BALB/c laboratory mice as expected, it did not replicate in the target organs of experimentally inoculated L. lakedownensis, indicating that the strict host specificity of MCMV may prevent its infection of L. lakedownensis. These results suggest that native mice on Thevenard Island are not at risk of MCMV infection from introduced house mice, and raise interesting questions about the possible selective survival of MCMV in small isolated populations of M. domesticus.     

Boa constrictor, an introduced predator threatening the endemic fauna on Cozumel Island, Mexico

We present evidence indicating that the boa (Boa constrictor) was introduced onto Cozumel Island, Quintana Roo, Mexico, in 1971. This snake is now firmly established and has a wide distribution on Cozumel. We recorded an encounter rate of 1.8 boas per 100 km of forest surveyed. The introduction of the boa onto Cozumel, where it has few predators, is a threat to the existence of endemic and other native terrestrial vertebrates of the island. We recommend the following immediate actions: (1) undertake research to confirm the origin of the boa on Cozumel; (2) assess the effect of the boa on the biota of Cozumel; and, (3) if an anthropogenic origin is confirmed, an eradication programme for the boa on Cozumel should be undertaken, obtaining information on the biology and ecology of the species. Options for the destiny of the eradicated boas are discussed.