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Using a polymerase chain reaction-based strategy, we have detected the expression of nine different homeobox genes in adult mouse intestine. Included among these are the recently described intestine-specific Cdx-1 gene and a new, related gene, Cdx-2. Southern blot experiments show that Cdx-2 is present in a single copy in the mouse genome. Of several adult mouse tissues assayed, intestine was the only one that contained detectable levels of Cdx-2 mRNA. Expression of all nine homeobox genes in different regions of the intestine was quantitated by RNase protection analysis, which revealed a unique expression profile for each gene. These observations suggest that homeobox gene expression may play an important role in cellular differentiation in the adult intestine. 相似文献
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Rathu Heenati (RHT) is a Sri Lankan rice cultivar that carries a brown planthopper (BPH) resistance gene, Bph3, and shows broad-spectrum resistance to all four biotypes of BPH. The BPH-resistance loci in RHT has been studied extensively
and assigned to four different rice chromosomes (3, 4, 6, and 10) by different research groups, but the gene has not been
cloned previously. An Affymetrix rice genome array containing 48,564 japonica and 1,260 indica sequences was used to analyze the potential resistance-related genes on the four chromosomes by comparative analysis of the
differentially expressed genes between resistant and susceptible rice cultivars exposed to BPH attack. The microarray results
showed that at least 17 genes related to induced resistance and at least 193 genes related to constitutive resistance in RHT.
On chromosome 3, the AOC4 was hypothesized to be the most important candidate gene. On chromosome 6, no valuable candidate resistance gene was identified
in the Bph3 localization region. In the three Quantitative trait locus regions of chromosomes 3, 4, and 10, the numbers of constitutive
and induced resistance-related genes found were 17, 26, and 12, respectively. The major probe on chromosome 10 represents
a constitutive expression gene with a very high absolute fold-change of 2,588.82. The microarray analysis indicated that BPH
resistance in RHT is probably controlled by a series of resistance-related genes. This study provides valuable information
for cloning, functional analysis and marker-assisted breeding of these BPH resistance genes. 相似文献
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Linkage, evolution, and expression of the rat apolipoprotein A-I, C-III, and A-IV genes 总被引:14,自引:0,他引:14
I A Haddad J M Ordovas T Fitzpatrick S K Karathanasis 《The Journal of biological chemistry》1986,261(28):13268-13277