共查询到20条相似文献,搜索用时 15 毫秒
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Karsten Meyer 《Mycotoxin Research》2011,27(4):303-306
Society for Mycotoxin Research – News and Announcements 2011/II
Report from the 33rd Mycotoxin-Workshop (Freising, Germany) 相似文献5.
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The present study was carried out to elucidate the effectiveness of curcumin in mitigating the adverse effects caused by N-Methyl N-Nitrosourea (MNU) on mouse cerebellum and cerebrum. Male laca mice received either intravenous MNU treatment at a dose of 10 mg/kg bw in sterile double distilled water, curcumin alone 60 mg/kg bw in drinking water, or combined MNU and curcumin treatment on alternate days for a period of 2 months. The effects of different treatments were studied on carbohydrate metabolizing enzymes viz: hexokinase, glucose-6-phosphatase (G6P), glucose-6-isomerase (G6I), lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and glycogen levels. Curcumin supplementation to MNU treated mice was able to reduce significantly the activities of the G6P, G6I, hexokinase, LDH, SDH and increased the glycogen contents in both the regions of brain which were altered following MNU treatment. Hence, curcumin shall prove to be effective in ameliorating the adverse effects caused by MNU. 相似文献
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The root endophytic fungus Piriformospora indica is a prime candidate to improve the growth and yield of plants. It also acts as a growth promoter and bioprotector, as well as combating environmental stress in a range of plant species. In the present investigation impact of a P. indica culture filtrate was studied on Helianthus annus Sun gold and H. annus Japanese gold varieties in the greenhouse. Treatment with the P. indica culture filtrate promoted overall growth and seed production of the plants. Moreover, the oil content of the seeds increased by 50% to 70% in these two varieties of H. annus plants. The possible reasons for these effects are discussed. 相似文献
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I compared life-history traits and self-fertilization rates in greenhouse culture of native Spartina alterniflora with an invasive population from a low-density Pacific estuary to see whether plants in the novel r-selective regime exhibit early reproduction, greater self-compatibility and high reproductive effort putting individuals at greater risk of death. Plants were grown from seed collected from the Atlantic and Gulf coasts of North America and a population introduced approximately 100 years ago to Willapa Bay, Washington, USA that has expanded to cover over ca. 6000 hectares. Pacific marshes have almost no native emergent vascular plants in the intertidal habitat, offering a virtually empty niche for invasive S. alterniflora. The low-density plants at the leading edge of this rapidly expanding population suffer a severe Allee effect of greatly reduced fecundity, caused by pollen limitation, compared to high-density areas. I found 99% of the invasive plants initiated reproduction in the first year of this study, while only 52% of the native range plants did so in the first year, followed by 34% in the second and 14% of native plants had not initiated reproduction after three years. The invasive plants had more than twice the reproductive effort of the native plants. Plants with the highest reproductive effort in the first year of growth died regardless of coastal site of origin, although nearly all of those that died over two years were invasive Pacific plants (27%). The invaders set two times the seed as the natives under forced selfing, suggesting greater self-compatibility or reproductive effort. These results suggest either a founding and bottleneck event and/or the invasive population has evolved from the long-lived, predominantly self-incompatible, K-selected state of the probable invasive propagules originating in dense, competitive native marshes.Co-ordinating editor: I. Olivieri 相似文献
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Silvia Flores-Benítez Juan F. Jiménez-Bremont Sergio Rosales-Mendoza Gerardo R. Argüello-Astorga Rosalba Castillo-Collazo Ángel Gabriel Alpuche-Solís 《Plant Cell, Tissue and Organ Culture》2007,91(3):215-224
Agave salmiana was transformed using two different protocols: co-cultivation with Agrobacterium tumefaciens and particle bombardment. The uidA (β-glucuronidase) gene was used as a reporter gene for both methods whereas the nptII and bar genes were used as selectable markers for A. tumefaciens and biolistic transformation respectively. Previous reports for in vitro regeneration of A. salmiana have not been published; therefore the conditions for both shoot regeneration and rooting were optimized using leaves and embryogenic calli of Agave salmiana. The transgenes were detected by Polymerase Chain Reaction (PCR) in 11 month old plants. The transgenic nature of the plants was also confirmed using GUS histochemical assays. Transformation via co-cultivation of explants with Agrobacterium harbouring the pBI121 binary vector was the most effective method of transformation, producing 32 transgenic plants and giving a transformation efficiency of 2.7%. On the other hand, the biolistic method produced transgenic calli that tested positive with the GUS assay after 14 months on selective medium while still undergoing regeneration. 相似文献
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L. B. Oknina O. A. Kuznetsova A. P. Belostotskyi N. L. Nechaeva E. V. Kutakova E. L. Masherow A. S. Romanov 《Human physiology》2011,37(1):49-56
The aim of the study was to trace the cumulative changes in the amplitude-time parameters of the AEP (acoustic evoked potential) components N1, N2, and P300 in the experimental situations of different complexities (counting and listening to sounds) and to compare the differences between young and mature subjects. The AEP was recorded in 12 healthy subjects aged 18 to 22 years and in 12 subjects aged 32 to 59 years. It was revealed that the components N1, N2, and P300 recorded in the situation of listening without any preliminary instruction did not differ in young and mature individuals. In the younger subjects, a higher N1 amplitude was revealed when they counted sounds, compared to listening, whereas the latency values of this component did not differ, irrespective of the complexity of the task. The component N2 was shown to demonstrate latency stability in relation to the age and the experimental situation. The N2 amplitude was higher in the situation of counting sounds in both age groups. The amplitude-time parameters of P300 did not differ in subjects of different ages in the listening task. The features of the components N1, N2, and P300 revealed in young and mature subjects in the experimental situations differing in complexity lead us to suggest that, with age, humans develop adaptive mechanisms contributing to successful task performance. 相似文献
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The encystment of Scrippsiella lachrymosa cells (strain B-10), which can be induced reliably in encystment medium, was inhibited by stirring the culture. 100 mL cultures in glass beakers were stirred at 1 rotation s−1. Stirring inhibited vegetative cells from congregating (swarming) at the walls of the culture container. When stirring was stopped, a rapid induction of sexual reproduction was seen. As soon as stirring stopped (within 2 min), cells were observed swarming near the edges of the glass beaker. Four days after cessation of stirring, large percentages of the cells were mating and, after 7 days, most were zygotes. Cultures were observed after 31, 38, and, 45 days of stirring. When cultures were stirred for 45 days, cysts developed in the stirred treatments, but these cysts were attached to flocculent material that had also formed in the medium. The use of this laboratory method is advantageous for the study of the mating through cyst stages of the dinoflagellate life history. This method may also demonstrate the need for a ‘surface’ as a place for the dinoflagellate to congregate in order to successfully encyst and may help explain environmental observations of encystment at pycnoclines. 相似文献
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Cryopreservation of Robinia pseudoacacia explants by vitrification achieved 78% survival following the stepwise preculture of shoot tips in (0.3 + 0.5 + 0.7 M) sucrose with a 80 min incubation in PVS2; compared to 87% survival after desiccation of explants to 30% water content, following 3 days alginate bead (with glycerol and sucrose treatments) preculture in 0.7 M sucrose. 相似文献
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Jiu-Cun Wang Syeling Lai Xinjian Guo Xuefeng Zhang Benoit de Crombrugghe Sonali Sonnylal Frank C Arnett Xiaodong Zhou 《Arthritis research & therapy》2010,12(2):R60
Introduction
SPARC is a matricellular protein, which, along with other extracellular matrix components including collagens, is commonly over-expressed in fibrotic diseases. The purpose of this study was to examine whether inhibition of SPARC can regulate collagen expression in vitro and in vivo, and subsequently attenuate fibrotic stimulation by bleomycin in mouse skin and lungs. 相似文献18.
B. Cukrowska I. Motyl H. Kozáková M. Schwarzer R. K. Górecki E. Klewicka K. Śliżewska Z. Libudzisz 《Folia microbiologica》2009,54(6):533-537
Three Lactobacillus strains (LOCK 0900, LOCK 0908, LOCK 0919) out of twenty-four isolates were selected according to their antagonistic activity against pathogenic bacteria, resistance to low pH and milieu of bile salts. Intragastric administration of a mixture of these strains to Balb/c mice affected cytokine TH1-TH2 balance toward nonallergic TH1 response. Spleen cells, isolated from lactobacilli-treated mice and re-stimulated in vitro with the mixture of heat-inactivated tested strains, produced significantly higher amounts of anti-allergic tumor necrosis factor- and interferon-γ than control animals whereas the level of pro-allergic interleukin-5 was significantly lower. Lactobacillus cells did not translocate through the intestinal barrier into blood, liver and spleen; a few Lactobacillus cells found in mesenteric lymph nodes could create antigenic reservoir activating the immune system. The mixture of Lactobacillus LOCK 0900, LOCK 0908 and LOCK 0919 strains represents a probiotic bacterial preparation with possible use in prophylaxis and/or therapy of allergic diseases. 相似文献
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Rosalia Crupi Daniela Impellizzeri Marika Cordaro Rosalba Siracusa Giovanna Casili Maurizio Evangelista Salvatore Cuzzocrea 《Molecular neurobiology》2018,55(11):8455-8472
Parkinson’s disease (PD) is a neurodegenerative disease characterized by degeneration of dopaminergic neurons. Aging is a major risk factor for idiopathic PD. Several prior studies examined the neuroprotective effects of palmitoylethanolamide (PEA), alone or combined with antioxidants, in a model of PD induced by the dopaminergic toxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Here, we analyzed the pretreatment effect of micronized PEA (PEAm) on neuroinflammation and neuronal cell death in the MPTP model. Male CD mice (21 months of age) were pre-treated for 60 days with PEAm. After this time, they received four intraperitoneal injections of MPTP over a 24-h period and were killed 7 days later. On the 8th day, brains were processed. Pretreatment with PEAm ameliorated behavioral deficits and the reductions in expression of tyrosine hydroxylase and dopamine transporter, while blunting the upregulation of α-synuclein and β3-tubulin in the substantia nigra after MPTP induction. Moreover, PEAm reduced proinflammatory cytokine expression and showed a pro-neurogenic effect in hippocampus. These findings propose this strategy as a valid approach to prevent neurodegenerative diseases associated with old age. 相似文献