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Abiotic stress protection by ecologically abundant dimethylsulfoniopropionate and its natural and synthetic derivatives: insights from Bacillus subtilis
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Sebastian Broy Chiliang Chen Tamara Hoffmann Nelson L. Brock Gabriele Nau‐Wagner Mohamed Jebbar Sander H. J. Smits Jeroen S. Dickschat Erhard Bremer 《Environmental microbiology》2015,17(7):2362-2378
Dimethylsulfoniopropionate (DMSP) is an abundant osmolyte and anti‐stress compound produced primarily in marine ecosystems. After its release into the environment, microorganisms can exploit DMSP as a source of sulfur and carbon, or accumulate it as an osmoprotectant. However, import systems for this ecophysiologically important compatible solute, and its stress‐protective properties for microorganisms that do not produce it are insufficiently understood. Here we address these questions using a well‐characterized set of Bacillus subtilis mutants to chemically profile the influence of DMSP import on stress resistance, the osmostress‐adaptive proline pool and on osmotically controlled gene expression. We included in this study the naturally occurring selenium analogue of DMSP, dimethylseleniopropionate (DMSeP), as well as a set of synthetic DMSP derivatives. We found that DMSP is not a nutrient for B. subtilis, but it serves as an excellent stress protectant against challenges conferred by sustained high salinity or lasting extremes in both low and high growth temperatures. DMSeP and synthetic DMSP derivatives retain part of these stress protective attributes, but DMSP is clearly the more effective stress protectant. We identified the promiscuous and widely distributed ABC transporter OpuC as a high‐affinity uptake system not only for DMSP, but also for its natural and synthetic derivatives. 相似文献
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为探究不同启动子对陆地棉GhCDPK1基因抗逆功能的影响,该研究克隆了长度为824bp和1 524bp的2个拟南芥RD29A的启动子序列,分别构建了35S启动子和2个RD29A启动子驱动的GhCDPK1融合表达载体,并利用农杆菌介导法转化烟草,分析了其驱动的转GhCDPK1基因烟草,在逆境胁迫处理后的表型变化,叶绿素、丙二醛(MDA)和脯氨酸含量,过氧化物酶(POD)和超氧化物歧化酶(SOD)活性以及细胞膜透性的生理变化。结果显示:RD29A启动子驱动的转GhCDPK1基因烟草,比35S启动子驱动表现出更强的耐逆性,其叶绿素含量、脯氨酸含量以及POD、SOD活性都高于35S启动子,而MDA含量与细胞膜的通透性低于35S启动子,且1 524bp的RD29A2启动子片段驱动转GhCDPK1基因烟草的耐胁迫能力比824bp启动子片段更强。 相似文献
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