共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
Autism spectrum disorders (ASD) are serious multisystem developmental disorders and an urgent global public health concern. Dysfunctional immunity and impaired brain function are core deficits in ASD. Aluminum (Al), the most commonly used vaccine adjuvant, is a demonstrated neurotoxin and a strong immune stimulator. Hence, adjuvant Al has the potential to induce neuroimmune disorders. When assessing adjuvant toxicity in children, two key points ought to be considered: (i) children should not be viewed as “small adults” as their unique physiology makes them much more vulnerable to toxic insults; and (ii) if exposure to Al from only few vaccines can lead to cognitive impairment and autoimmunity in adults, is it unreasonable to question whether the current pediatric schedules, often containing 18 Al adjuvanted vaccines, are safe for children? By applying Hill's criteria for establishing causality between exposure and outcome we investigated whether exposure to Al from vaccines could be contributing to the rise in ASD prevalence in the Western world. Our results show that: (i) children from countries with the highest ASD prevalence appear to have the highest exposure to Al from vaccines; (ii) the increase in exposure to Al adjuvants significantly correlates with the increase in ASD prevalence in the United States observed over the last two decades (Pearson r = 0.92, p < 0.0001); and (iii) a significant correlation exists between the amounts of Al administered to preschool children and the current prevalence of ASD in seven Western countries, particularly at 3-4 months of age (Pearson r = 0.89-0.94, p = 0.0018-0.0248). The application of the Hill's criteria to these data indicates that the correlation between Al in vaccines and ASD may be causal. Because children represent a fraction of the population most at risk for complications following exposure to Al, a more rigorous evaluation of Al adjuvant safety seems warranted. 相似文献
3.
A new chemiluminescence (CL) system based on the reaction of Ag(III) complex with luminol is, for the first time, reported in this work. Incorporated with a flow injection analyses (FIA), the new CL system has been applied for the determination of free cortisol in human sera. The system is based on the CL reaction of luminol with Ag(III) in alkaline solutions, while cortisol can dramatically enhance CL intensities. Under optimum conditions, CL intensities are proportional to concentration of cortisol in the range of 0.05-7.5 nM. The limit of detection is 2.0 × 10−11 M (3σ), with a relative standard deviation (n = 11) of 1.9% for 3.5 × 10−9 M cortisol. Eight human blood serum samples were all handled by solid-phase extraction (SPE) clean-up and enrichment before detection. This detection system is highly sensitive and convenient and may find wide applications. Based on the chemiluminescent spectra, a possible reaction mechanism is also suggested. 相似文献
4.
Sushil Kumar Vinod Kumar Dixit 《Biochemical and biophysical research communications》2009,387(3):456-460
Helicobacter pylori infection is strongly associated with gastric cancer. In the present study, the relationship between interleukin-1B (IL-1B) polymorphism, H. pylori infection, and prevalence of gastric cancer (GC) in patients of North India was evaluated using genomic DNA directly extracted from biopsy tissues for performing PCR-RFLP. A total of 136 GC cases and 110 healthy controls were included for studying polymorphisms in the genotypes of IL-1B−511, −31, +3954 and IL-1RN both in the presence and absence of H. pylori active infection. Results showed that the frequency of IL-1RN 2/2 was significantly higher in GC cases (21.32%) than the controls (9.09%) with an odds ratio (OR) of 4.391 (95% CI 1.093-10.131). The risk of GC was also found higher in other genotypes of IL-1B namely, −511 TT (χ2 = 18.975, p < 0.001), −31CC (χ2 = 21.219, p < 0.001), +3954 CT (χ2 = 21.082, p < 0.001) and IL-1RN 1/2 (χ2 = 30.543, p < 0.001) with active infection of H. pylori. Our findings indicate that the IL-1B and IL-1RN polymorphisms are associated with the development of GC and H. pylori infection markedly increases the risk of GC in North Indian population. Additionally, IL-1B−511 C/C and IL-RN 2/2 polymorphisms seem to be involved in the development of GC in H. pylori uninfected patients. 相似文献
5.
Panchangam Murali Krishna 《Inorganica chimica acta》2009,362(11):4185-4190
A copper(I) complex [Cu(CETH)2Cl] (Ia), where CETH = cuminaldehyde-4-ethyl-3-thiosemicarbazone (I), is prepared and structurally characterised. The complex crystallizes in orthorhombic space group pna2(1) with the unit cell parameters; a = 9.8598(14) Å, b = 15.411(2) Å, c = 0.817(3) Å, V = 2055.9(4) Å3 and Z = 4. The neutral complex has the copper(I) centre bonded to two thioketonic sulphur donor in η1-S bonding mode and one chloride giving ‘Y’ shape geometry. The complex is diamagnetic and exhibits a copper to ligand charge transfer bands at 351 and 398 nm in dimethylformamide. The complex shows quasireversible cyclic voltammetric response at 0.41 V (ΔEp = 300 mV) at 50 mVS−1 in DMF for the Cu(II)/Cu(I) oxidation couple. Complex Ia shows marginal nuclease activity with pUC18 DNA in the presence of reducing agent (Dithiotretal) and hydrogen peroxide. 相似文献
6.
Mixed ligand complexes: [Co(L)(bipy)] · 3H2O (1), [Ni(L)(phen)] · H2O (2), [Cu(L)(phen)] · 3H2O (3) and [Zn(L)(bipy)] · 3H2O (4), where L2− = two -COOH deprotonated dianion of N-(2-benzimidazolyl)methyliminodiacetic acid (H2bzimida, hereafter, H2L), bipy = 2,2′ bipyridine and phen = 1,10-phenanthroline have been isolated and characterized by elemental analysis, spectral and magnetic measurements and thermal studies. Single crystal X-ray diffraction studies show octahedral geometry for 1, 2 and 4 and square pyramidal geometry for 3. Equilibrium studies in aqueous solution (ionic strength I = 10−1 mol dm−3 (NaNO3), at 25 ± 1 °C) using different molar proportions of M(II):H2L:B, where M = Co, Ni, Cu and Zn and B = phen, bipy and en (ethylene diamine), however, provides evidence of formation of mononuclear and binuclear binary and mixed ligand complexes: M(L), M(H−1L)−, M(B)2+, M(L)(B), M(H−1L)(B)−, M2(H−1L)(OH), (B)M(H−1L)M(B)+, where H−1L3− represents two -COOH and the benzimidazole N1-H deprotonated quadridentate (O−, N, O−, N), or, quinquedentate (O−, N, O−, N, N−) function of the coordinated ligand H2L. Binuclear mixed ligand complex formation equilibria: M(L)(B) + M(B)2+ ? (B)M(H−1L)M(B)+ + H+ is favoured with higher π-acidity of the B ligands. For Co(II), Ni(II) and Cu(II), these equilibria are accompanied by blue shift of the electronic absorption maxima of M(II) ions, as a negatively charged bridging benzimidazolate moiety provides stronger ligand field than a neutral one. Solution stability of the mixed ligand complexes are in the expected order: Co(II) < Ni(II) < Cu(II) > Zn(II). The Δ log KM values are less negetive than their statistical values, indicating favoured formation of the mixed ligand complexes over the binary ones. 相似文献
7.
Elena Larrieta-Carrasco Paola León-Mimila Teresa Villarreal-Molina Hugo Villamil-Ramírez Sandra Romero-Hidalgo Leonor Jacobo-Albavera Roxana Gutiérrez-Vidal Blanca E. López-Contreras Luz E. Guillén-Pineda Fausto Sánchez-Muñoz Rafael Bojalil Ana M. Mejía-Domínguez Nahúm Méndez-Sánchez Aaron Domínguez-López Carlos A. Aguilar-Salinas Samuel Canizales-Quinteros 《Gene》2013
Background and aims
Non-alcoholic fatty liver disease (NAFLD) and elevated alanine transaminase (ALT) levels are common in obese Hispanic adults and children. Recently, a PNPLA3 gene variant (I148M) was strongly associated with NAFLD and higher ALT levels in obese adults, including Hispanics. The aims of this study were to estimate the frequency of elevated ALT levels, and to address the influence of obesity and PNPLA3/I148M on ALT levels in a general population sample of Mexican school-aged children.Methods
A total of 1037 non-related Mexican children aged 6 to 12 years were genotyped for the I148M variant. Anthropometric, clinical and metabolic parameters were collected from all participants.Results
Elevated ALT levels (> 35 U/L) were more frequent in obese (26.9%) and overweight (9.3%) than in normal weight children (2.2%). The M148M genotype was significantly associated with elevated ALT levels in this population (OR = 3.7, 95% CI 2.3–5.9; P = 3.7 × 10− 8), and children carrying the M148M genotype showed significantly lower HDL cholesterol levels and BMI z-core (P = 0.036 and 0.015, respectively). On stratifying by BMI percentile, this genotype conferred a much greater risk of elevated ALT levels in normal weight (OR = 19.9, 95% CI 2.5–157.7; P = 0.005) than overweight and obese children (OR = 3.4, 95% CI 1.3–8.9; P = 0.014 and OR = 3.1, 95% CI 1.7–5.5; P = 1.4 x10− 4, respectively).Conclusions
The I148M PNPLA3 variant is strongly associated with elevated ALT levels in normal weight and overweight/obese Mexican children. Thus, the M148M genotype may be considered as an important risk factor for liver damage in this population. 相似文献8.
Pascal V. Grundler 《Inorganica chimica acta》2006,359(6):1795-1806
The ruthenium(II) hexaaqua complex [Ru(H2O)6]2+ reacts with dihydrogen under pressure to give the η2-dihydrogen ruthenium(II) pentaaqua complex [Ru(H2)(H2O)5]2+.The complex was characterized by 1H, 2H and 17O NMR: δH = −7.65 ppm, JHD = 31.2 Hz, δO = −80.4 ppm (trans to H2) and δO = −177.4 ppm (cis to H2).The H-H distance in coordinated dihydrogen was estimated to 0.889 Å from JHD, which is close to the value obtained from DFT calculations (0.940 Å).Kinetic studies were performed by 1H and 2H NMR as well as by UV-Vis spectroscopy, yielding the complex formation rate and equilibrium constants: kf = (1.7 ± 0.2) × 10−3 kg mol−1 s−1 and Keq = 4.0 ± 0.5 mol kg−1.The complex formation rate with dihydrogen is close to values reported for other ligands and thus it is assumed that the reaction with dihydrogen follows the same mechanisn (Id).In deuterated water, one can observe that [Ru(H2)(H2O)5]2+ catalyses the hydrogen exchange between the solvent and the dissolved dihydrogen.A hydride is proposed as the intermediate for this exchange.Using isotope labeling, the rate constant for the hydrogen exchange on the η2-dihydrogen ligand was determined as k1 = (0.24 ± 0.04) × 10−3 s−1.The upper and lower limits of the pKa of the coordinated dihydrogen ligand have been estimated:3 < pKa < 14. 相似文献
9.
Aims
We evaluated the mechanisms involved in insulin-induced vasodilatation after acute resistance exercise in healthy rats.Main methods
Wistar rats were divided into 3 groups: control (CT), electrically stimulated (ES) and resistance exercise (RE). Immediately after acute RE (15 sets with 10 repetitions at 70% of maximal intensity), the animals were sacrificed and rings of mesenteric artery were mounted in an isometric system. After this, concentration–response curves to insulin were performed in control condition and in the presence of LY294002 (PI3K inhibitor), L-NAME (NOS inhibitor), L-NAME + TEA (K+ channels inhibitor), LY294002 + BQ123 (ET-A antagonist) or ouabain (Na+/K+ ATPase inhibitor).Key findings
Acute RE increased insulin-induced vasorelaxation as compared to control (CT: Rmax = 7.3 ± 0.4% and RE: Rmax = 15.8 ± 0.8%; p < 0.001). NOS inhibition reduced (p < 0.001) this vasorelaxation from both groups (CT: Rmax = 2.0 ± 0.3%, and RE: Rmax = − 1.2 ± 0.1%), while PI3K inhibition abolished the vasorelaxation in CT (Rmax = − 0.1 ± 0.3%, p < 0.001), and caused vasoconstriction in RE (Rmax = − 6.5 ± 0.6%). That insulin-induced vasoconstriction on PI3K inhibition was abolished (p < 0.001) by the ET-A antagonist (Rmax = 2.9 ± 0.4%). Additionally, acute RE enhanced (p < 0.001) the functional activity of the ouabain-sensitive Na+/K+ ATPase activity (Rmax = 10.7 ± 0.4%) and of the K+ channels (Rmax = − 6.1 ± 0.5%; p < 0.001) in the insulin-induced vasorelaxation as compared to CT.Significance
Such results suggest that acute RE promotes enhanced insulin-induced vasodilatation, which could act as a fine tuning to vascular tone. 相似文献10.
Paolo Ascenzi Alessandra di Masi Massimo Coletta 《Biochemical and biophysical research communications》2009,387(1):83-189
Human serum albumin (HSA) is a monomeric allosteric protein. Here, the effect of ibuprofen on denitrosylation kinetics (koff) and spectroscopic properties of HSA-heme-Fe(II)-NO is reported. The koff value increases from (1.4 ± 0.2) × 10−4 s−1, in the absence of the drug, to (9.5 ± 1.2) × 10−3 s−1, in the presence of 1.0 × 10−2 M ibuprofen, at pH 7.0 and 10.0 °C. From the dependence of koff on the drug concentration, values of the dissociation equilibrium constants for ibuprofen binding to HSA-heme-Fe(II)-NO (K1 = (3.1 ± 0.4) × 10−7 M, K2 = (1.7 ± 0.2) × 10−4 M, and K3 = (2.2 ± 0.2) × 10−3 M) were determined. The K3 value corresponds to the value of the dissociation equilibrium constant for ibuprofen binding to HSA-heme-Fe(II)-NO determined by monitoring drug-dependent absorbance spectroscopic changes (H = (2.6 ± 0.3) × 10−3 M). Present data indicate that ibuprofen binds to the FA3-FA4 cleft (Sudlow’s site II), to the FA6 site, and possibly to the FA2 pocket, inducing the hexa-coordination of HSA-heme-Fe(II)-NO and triggering the heme-ligand dissociation kinetics. 相似文献
11.
K.L. Scales J.P. Lewis D. Castellanos R.T. Graham 《Journal of experimental marine biology and ecology》2011,407(1):122-129
Patterns of distribution, key biometric parameters and home range extent were determined for hawksbill turtles at Lighthouse Reef Atoll (LRA), Belize over two field seasons (16 days, 2009; 30 days, 2010). Relative abundance was determined using 49 sightings transects (≈ 1 km) distributed across the atoll and of all turtles encountered (n = 68), 91% were immature (CCLmin ≤ 65 cm). Habitat type was significantly correlated with abundance, with more turtles encountered on the coral reef than in the lagoon (GzLMM, χ22 = 6.85, p < 0.05; CPUE reef = 1.41 turtles h− 1, CPUE lagoon = 0.62 turtles h− 1). Hawksbills were also significantly more abundant within protected areas (GzLMM, χ21 = 8.69, p < 0.05; CPUE Blue Hole Natural Monument (BHNM) = 2.96 turtles person− 1 h− 1; CPUE Half Moon Caye Natural Monument (HMCNM) = 2.34 turtles h− 1; outside boundaries = 0.88 turtles h− 1). Of 26 captures, 19 focal individuals were equipped with ultrasonic transmitters for active acoustic telemetry, and tracked for 6-25 days (n = 10, 2009; n = 9, 2010). Spatial habitat utilisation was found to be highly variable, with large areas of overlap between distinct home ranges. Home range averaged 31.2 ha ± 32.6 (range 5.1-111.3 ha) for the juveniles that were successfully tracked (n = 15), with maximum displacement in the order of 1.8 km ± 1.0 (range 0.5-4.0 km) and net displacement at 1.2 km ± 0.9. This offshore atoll constitutes an important developmental habitat for the regional population and although our tracking durations were limited, home range of juvenile hawksbills at this site is significantly more expansive than that documented elsewhere. 相似文献
12.
Jovana Karoline Lima Neiva Leite Luciane Viater Turek Ricardo Lehtonen Rodrigues Souza Luciana da Silva Timossi Ana Claudia Vecchi Osiecki Raul Osiecki Lupe Furtado-Alle 《Gene》2013
Polymorphisms of butyrylcholinesterase (BChE) have been reported to be associated to weight, BMI variance and hypertriglyceridemia in adults and adolescents. The aim of the present study was to investigate the association of −116A (SNP: G/A; rs1126680) and 1914G (SNP: A/G; rs3495) variants of BCHE gene with anthropometric and biochemical variables associated with obesity in population sample of 115 individuals, from Southern Brazil. Participants were grouped in two categories: obese (BMI ≥ 30) and non-obese (BMI < 30). The 1914G allele showed significantly higher frequency in the obese group, and carriers of 1914G allele showed lower mean BChE activity when compared to 1914A carriers (p = 0.006). Higher means of BMI (p = 0.02) and triglyceride (TG; p = 0.01) were found in 1914G carriers (BMI = 27.57kg/m2; TG = 150.8 mg/dL) when compared to 1914A homozygotes (BMI = 25.55 kg/m2; TG = 107.9 mg/dL). Carriers of the −116A allele showed lower mean BChE activity than usual homozygotes, and the −116A variant was found in cis with 1914G (p < 0.0001; D′ = 1). The region of BCHE gene that contains the 1914G mutation site is target of microRNAs (miRs) and the response of BChE to glucocorticoids is especially influenced by these miRs. Therefore, it is possible that the 1914G allele can be interfering in gluconeogenesis, hyperglycemia, lipolysis and body fat distribution. This lower activity may cause an imbalance in lipid metabolism, which may lead to an increased predisposition to obesity and to a lower ability to maintain metabolic homeostasis. 相似文献
13.
Wei Xu 《Inorganica chimica acta》2011,365(1):297-301
Reaction of fresh Mn(OH)2 precipitate and S-carboxymethyl-l-cysteine (H2SCMC) in aqueous solution afforded a novel chiral 3D coordination polymer Mn(H2O)(SCMC) 1, which crystallizes in the acentric polar space group P21 with cell constants a = 5.079(1) Å, b = 9.617(2) Å, c = 8.649(2) Å, β = 94.40(3)°, V = 421.2(1) Å3, Z = 2, and exhibit a SHG effect and ferroelectricity (a remnant polarization Pr = 0.0159 uC cm−2, coercive field Ec = 0.83 kV cm−2, saturation of the spontaneous polarization Ps = 0.234 uC cm−2). To the best of our knowledge, the present compound represents the first example of S-carboxymethyl-l-cysteine coordination polymers that exhibit possible ferroelectric behavior. The structural analysis revealed that the Mn2+ ions in 1 are each coordinated by one N atom and five O atoms of four S-carboxymethyl-l-cysteine ligand bridges four symmetry-related Mn2+ ions to form 3D MOF of 66 topology type with irregular chiral channels extending along [1 0 0]. The temperature-dependent magnetic susceptibilities shows that 1 obeys Curie-Weiss law χm = C/(T − Θ) with C = 4.23 cm3 mol−1 K and Θ = −5.86 K and the best fit gave a weak antiferromagnetic coupling (J = −0.282(5) cm−1) among Mn ions. 相似文献
14.
Susan Kidd Paula Midgley Nazir Lone Mary Nicol John Smith Neil McIntosh 《Steroids》2009,74(8):666-1890
Background
The use of saliva for measurement of cortisol permits non-invasive study of adrenal function, but collection can be technically difficult, particularly in small infants. Saliva collection can be assisted by citric acid to increase saliva flow, or by the use of cotton or polyester swabs in the mouth.Aim
To determine whether different methods of saliva collection affect cortisol radioimmunoassay (RIA) performance.Experimental
Cortisol was measured in saliva collected from 16 adults using intra-oral cotton swabs or polyester swabs, compared with saliva dribbled directly into a pot either alone (plain saliva) or after citric acid had been placed on the tongue. An in-house RIA, without prior extraction, was used to measure cortisol with an encapsulated sheep antibody.Results
Mean (median) salivary cortisol was 10.9 (10.5) nmol L−1 in plain saliva, 10.4 (8.4) nmol L−1 in citric acid stimulated saliva; 25.3 (25.1) nmol L−1 in saliva collected on cotton swabs, and 27.9 (27.3) nmol L−1 collected on polyester swabs. Cortisol in saliva collected using citric acid was not significantly different from plain saliva (p = 0.997), but cortisol in saliva collected using cotton and polyester swabs was significantly higher than that of plain saliva (p < 0.01).Conclusion
The use of cotton or polyester swabs for collection of saliva can result in spuriously high levels of cortisol when measured by RIA. 相似文献15.
Yousri M. Hussein Sally M. Shalaby Amani Nassar Saad S. Alzahrani Ayman S. Alharbi Maha Nouh 《Gene》2014
Objective
To determine whether IL-4, IL-4Rα and STAT6 polymorphisms are associated with susceptibility to dermatitis in Egyptian children.Methods
We genotyped three groups of children, consisting of 106 atopic dermatitis (AD) children, 95 non-AD children, and 100 of healthy controls, for IL-4 (− 590 C/T), (− 33 C/T), IL-4Rα (I50V), (Q576R) and STAT6 (2964 G/A), (2892 C/T) gene polymorphisms using PCR-RFLP assay. Total serum IgE and serum IL-4 levels were detected by ELISA.Results
There was a non-significant association of IL-4 − 590 C/T, − 33 C/T polymorphisms in the children with non-AD or those with AD when compared with the controls. We identified a significant association between IL-4Rα I50V, Q576R polymorphisms and dermatitis susceptibility in AD (p = 0.002, < 0.001 respectively), whereas no such association was observed in non-AD group (p = 0.52, 0.99 respectively). A significant association between STAT6 polymorphisms and both types of dermatitis was found. Patients who were carriers of IL4 − 590C, IL-4Rα I50V G, STAT6 2964 A and STAT6 2892 T had an increased risk of AD [OR and 95% CI: 3.2 (2.5–4.2), p = 0.005]. Furthermore, there was no relation between each polymorphism and serum IL-4 level (p > 0.05 for each) while homozygosity for the risk alleles of IL-4, IL-4Rα and STAT6 SNPs were significantly associated with increased total IgE levels in all subjects.Conclusion
In Egyptian children, the IL-4Rα and the STAT6 polymorphism may play a role in susceptibility to AD. In addition, gene–gene interaction between the IL-4, the IL-4Rα and the STAT6 significantly increases an individual's susceptibility to AD. 相似文献16.
Ulrike Weik 《Hormones and behavior》2010,58(3):405-409
Though postawakening cortisol is considered to be altered under chronic stress prospective studies proving this assumption is missing, so far. Furthermore, there is some uncertainty which aspects of postawakening cortisol alterations are strongest related to stress. The present study thus analyzed the cortisol concentration at awakening itself (0 min), the cortisol awakening response (CAR; i.e. the increase within 30 min after awakening), the area under the curve of the first hour after awakening (AUCG60) and the mean of samples taken 0 min and 30 min after awakening (AUCG30) in 12 exam students, participating in a major exam and 12 matched control students not participating in any exam. Saliva samples were taken on two consecutive days at 0, 15, 30, 45, and 60 min after awakening, respectively, at four time points (T1-T4): on the verge of exams, when students anticipated and prepared the exam (T1), in the middle of exams (T2), and shortly after (T3). T4 (weeks after exams) represents a reference measure. Repeated measures analyses of covariance revealed a significantly higher AUCG30 (p = 0.007) and AUCG60 (p = 0.011) and higher cortisol concentrations at awakening (p = 0.016) in exam students and a significant time by group interaction for concentration at awakening (p = 0.031). No effects were found for the CAR. The results of this prospective controlled study support notions that chronic stress induces increases of overall postawakening cortisol. They further indicate that the CAR is not affected by chronic stress and that the awakening concentration responds later than the AUCG to conditions of chronic stress as analyzed here. 相似文献
17.
Meng Zhang Te Cao Leyi Ni Ping XieGuorong Zhu Aiwen ZhongJun Xu Hui Fu 《Aquatic Botany》2011,94(4):151-157
The uptake kinetics of phosphate (Pi) by Myriophyllum spicatum was determined from adsorption and absorption under light and dark conditions. Pi uptake was light dependent and showed saturation following the Michaelis-Menten relation (in light: V = 16.91 × [Pi](1.335 + [Pi]), R2 = 0.90, p < 0.001; in the dark: V = 5.13 × [Pi](0.351 + [Pi]), R2 = 0.77, p < 0.001). Around 77% of the loss of Pi in the water column was absorbed into the tissue of M. spicatum, and only 23% was adsorbed on the surface of the plant shoots. Our study shows that M. spicatum shoots have a much higher affinity (in light: 3.9 μmol g−1 dw h−1 μM−1; in the dark: 3.7 μmol g−1 dw h−1 μM−1) and Vmax (maximum uptake rate, shoot light) for Pi uptake than many other aquatic macrophytes (in light: 0.002-0.23 μmol g−1 dw h−1 μM−1; in the dark: 0.002-0.19 μmol g−1 dw h−1 μM−1), which may provide a competitive advantage over other macrophytes across a wide range of Pi concentrations. 相似文献
18.
Waqas Ahmed Imran Saeed Ali Moeen Riaz Asma Younas Ahmed Sadeque Asfandyar Khan Niazi Saad Hameed Niazi Syeda Hafiza Benish Ali Maleeha Azam Raheel Qamar 《Gene》2013
Single nucleotide polymorphisms (SNPs) of non-coding RNA in the INK4 locus (ANRIL) have been found to be associated with myocardial infarction (MI). However, the effect of rs1333049:C>G in INK4 locus in familial hypercholesterolemia patients and on lipid profile of the patients has not been studied in Pakistan. We therefore investigated the association of SNP rs1333049:C>G with MI as well as familial hypercholesterolemia patients and also determined the effect of genotype on lipid levels in a northern Pakistani population. A case–control association study was performed in which 611 individuals (294 patients, 290 healthy controls and 27 patients from hypercholesterolemia families) were genotyped for rs1333049:C>G, using an Allele specific polymerase chain reaction. We found a significant association of rs1333049:C>G with MI (χ2 = 22.3, p < 0.001). The frequency of risk genotype CC was significantly different from the healthy controls (p < 0.001, χ2 = 22.3). The risk allele C was at a higher frequency in the MI patients as compared to the controls (odds ratio [OR] = 1.55 (95% confidence interval [CI] = 1.22–1.96), p < 0.001). The logistic regression analysis for the genotype distribution resulted in strong association of risk allele C with MI under recessive model (OR = 3.17 (95% CI = 1.85–5.44) p < 0.001). When the data were further analyzed along the lines of gender, a significant association with both males and females was observed. 相似文献
19.
Nilusha Ragunathan Benjamin Pluvinage Elodie Sanfins Fernando Rodrigues-Lima Julien Dairou 《FEBS letters》2010,584(15):3366-29
Human arylamine N-acetyltransferase 1 (NAT1) is a xenobiotic-metabolizing enzyme that biotransforms aromatic amine chemicals. We show here that biologically-relevant concentrations of inorganic (Hg2+) and organic (CH3Hg+) mercury inhibit the biotransformation functions of NAT1. Both compounds react irreversibly with the active-site cysteine of NAT1 (half-maximal inhibitory concentration (IC50) = 250 nM and kinact = 1.4 × 104 M−1 s−1 for Hg2+ and IC50 = 1.4 μM and kinact = 2 × 102 M−1 s−1 for CH3Hg+). Exposure of lung epithelial cells led to the inhibition of cellular NAT1 (IC50 = 3 and 20 μM for Hg2+ and CH3Hg+, respectively). Our data suggest that exposure to mercury may affect the biotransformation of aromatic amines by NAT1. 相似文献
20.
Ashish Kumar Singh Sanjay Kumar Singh Daya Shankar Pandey 《Inorganica chimica acta》2010,363(5):995-1000
Synthesis of complexes with the formulations [M(CPI)2Cl2] (M = Zn, 1; M = Cd, 4) and [M(CPI)6](X)2 (M = Zn, X = NO3−, 2; X = ClO4−, 3; M = Cd, X = NO3−, 5; X = ClO4−, 6) have been achieved from the reactions of MCl2, M(NO3)2·xH2O and M(ClO4)2·xH2O (M = Zn, Cd) with 1-(4-cyanophenyl)-imidazole (CPI). Complexes 1-6 have been characterized by elemental analyses and spectral studies (IR, 1H, 13C NMR, electronic absorption and emission). Molecular structures of 1, 2, 3 and 6 have been determined crystallographically. Weak interaction studies on the complexes revealed presence of various interesting motifs resulting from C-H···N, C-H···Cl and π-π stacking interactions. The complexes under study exhibit strong luminescence at ∼450 nm in DMSO at room temperature. 相似文献