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Shengmin An Yue Liu Kangqi Sang Ting Wang Jingquan Yu Yanhong Zhou Xiaojian Xia 《植物学报(英文版)》2023,65(1):10-24
Brassinosteroids (BRs) and abscisic acid (ABA) are essential regulators of plant growth and stress tolerance. Although the antagonistic interaction of BRs and ABA is proposed to ensure the balance between growth and defense in model plants, the crosstalk between BRs and ABA in response to chilling in tomato (Solanum lycopersicum), a warm-climate horticultural crop, is unclear. Here, we determined that overexpression of the BR biosynthesis gene DWARF (DWF) or the key BR signaling gene BRASSINAZOLE-RESISTANT1 (BZR1) increases ABA levels in response to chilling stress via positively regulating the expression of the ABA biosynthesis gene 9-CIS-EPOXYCAROTENOID DIOXYGENASE1 (NCED1). BR-induced chilling tolerance was mostly dependent on ABA biosynthesis. Chilling stress or high BR levels decreased the abundance of BRASSINOSTEROID-INSENSITIVE2 (BIN2), a negative regulator of BR signaling. Moreover, we observed that chilling stress increases BR levels and results in the accumulation of BZR1. BIN2 negatively regulated both the accumulation of BZR1 protein and chilling tolerance by suppressing ABA biosynthesis. Our results demonstrate that BR signaling positively regulates chilling tolerance via ABA biosynthesis in tomato. The study has implications in production of warm-climate crops in horticulture. 相似文献
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Non‐enzymatic antioxidant accumulations in BR‐deficient and BR‐insensitive barley mutants under control and drought conditions
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Damian Gruszka Anna Janeczko Michal Dziurka Ewa Pociecha Jozsef Fodor 《Physiologia plantarum》2018,163(2):155-169
Drought is one of the most adverse stresses that affect plant growth and yield. Disturbances in metabolic activity resulting from drought cause overproduction of reactive oxygen species. It is postulated that brassinosteroids (BRs) regulate plant tolerance to the stress conditions, but the underlying mechanisms remain largely unknown. An involvement of endogenous BRs in regulation of the antioxidant homeostasis is not fully clarified either. Therefore, the aim of this study was to elucidate the role of endogenous BRs in regulation of non‐enzymatic antioxidants in barley (Hordeum vulgare) under control and drought conditions. The plant material included the ‘Bowman’ cultivar and a group of semi‐dwarf near‐isogenic lines (NILs), representing mutants deficient in BR biosynthesis or signaling. In general, accumulations of 11 compounds representing various types of non‐enzymatic antioxidants were analyzed under both conditions. The analyses of accumulations of reduced and oxidized forms of ascorbate indicated that the BR mutants contain significantly higher contents of dehydroascorbic acid under drought conditions when compared with the ‘Bowman’ cultivar. The analysis of glutathione accumulation indicated that under the control conditions the BR‐insensitive NILs contained significantly lower concentrations of this antioxidant when compared with the rest of genotypes. Therefore, we postulate that BR sensitivity is required for normal accumulation of glutathione. A complete accumulation profile of various tocopherols indicated that functional BR biosynthesis and signaling are required for their normal accumulation under both conditions. Results of this study provided an insight into the role of endogenous BRs in regulation of the non‐enzymatic antioxidant homeostasis. 相似文献
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Choe S Schmitz RJ Fujioka S Takatsuto S Lee MO Yoshida S Feldmann KA Tax FE 《Plant physiology》2002,130(3):1506-1515
Mutants defective in the biosynthesis or signaling of brassinosteroids (BRs), plant steroid hormones, display dwarfism. Loss-of-function mutants for the gene encoding the plasma membrane-located BR receptor BRI1 are resistant to exogenous application of BRs, and characterization of this protein has contributed significantly to the understanding of BR signaling. We have isolated two new BR-insensitive mutants (dwarf12-1D and dwf12-2D) after screening Arabidopsis ethyl methanesulfonate mutant populations. dwf12 mutants displayed the characteristic morphology of previously reported BR dwarfs including short stature, short round leaves, infertility, and abnormal de-etiolation. In addition, dwf12 mutants exhibited several unique phenotypes, including severe downward curling of the leaves. Genetic analysis indicates that the two mutations are semidominant in that heterozygous plants show a semidwarf phenotype whose height is intermediate between wild-type and homozygous mutant plants. Unlike BR biosynthetic mutants, dwf12 plants were not rescued by high doses of exogenously applied BRs. Like bri1 mutants, dwf12 plants accumulated castasterone and brassinolide, 43- and 15-fold higher, respectively, providing further evidence that DWF12 is a component of the BR signaling pathway that includes BRI1. Map-based cloning of the DWF12 gene revealed that DWF12 belongs to a member of the glycogen synthase kinase 3beta family. Unlike human glycogen synthase kinase 3beta, DWF12 lacks the conserved serine-9 residue in the auto-inhibitory N terminus. In addition, dwf12-1D and dwf12-2D encode changes in consecutive glutamate residues in a highly conserved TREE domain. Together with previous reports that both bin2 and ucu1 mutants contain mutations in this TREE domain, this provides evidence that the TREE domain is of critical importance for proper function of DWF12/BIN2/UCU1 in BR signal transduction pathways. 相似文献
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Brassinosteroids (BRs) are a family of plant steroid hormones that play diverse roles in many aspects of plant growth and development. For example, BRs promote seed germination by counteracting the inhibitory effect of ABA and regulate plant reproductive development, thus affecting seed yield. We have recently reported that MOTHER OF FT AND TFL1 (MFT) regulates seed germination through a negative feedback loop modulating ABA signaling in Arabidopsis. Here, we show that MFT function is also relevant to the BR signaling pathway. In mft loss-of-function mutants, the application of BR could not fully antagonize the inhibitory effect of exogenous ABA on seed germination, suggesting that BR promotes seed germination against ABA partly through MFT. In addition, mft enhances the low-fertility phenotype of det2 in which BR biosynthesis is blocked. This phenotype, together with the observation that MFT is expressed in gametophytes and developing seeds, suggests that MFT and BR play redundant roles in regulating fertility. Therefore, these results suggest that MFT affects seed germination and fertility relevant to the BR signaling pathway.Key words: Arabidopsis, brassinosteroid, abscisic acid, fertility, seed germinationPlant hormones exert profound effects on many fundamental processes during plant growth and development. With respect to seed development and germination, it has long been known that abscisic acid (ABA) and gibberellin (GA) are two major types of phytohormones that play antagonistic roles in regulating these events. Not until recently, another group of phytohormones, namely brassinosteroids (BRs), has also been found to counteract the inhibitory effect of ABA on seed germination.1,2 In addition, BR has been suggested to act in parallel with GA to promote cell elongation and germination.1,3,4BRs are a class of polyhydroxysteroids that are found in a wide variety of plant species.5 They can be detected in almost every plant tissue, with the highest abundance in the pollen and seeds.6 The most active component in the family of BRs is 24-epibrassinolide (BL), which is capable of activating BR signaling.6 In Arabidopsis, when the early steps of BR biosynthesis are blocked, the resulting defects include reduced male fertility under normal growth conditions7,8 and decreased germination percentage in the presence of exogenous ABA.1 Thus, BR plays an indispensible role in the control of seed development and also contributes to the regulation of seed germination.We have previously reported that MOTHER OF FT AND TFL1 (MFT) responds to both ABA and GA signals to regulate seed germination.9 Here we show that MFT functions in regulating seed germination and fertility, which is also relevant to the BR signaling pathway. Thus, MFT seems to function specifically in seeds in response to various phytohormones. 相似文献
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Qiannan Wang Shufen Wang Sufu Gan Xin Wang Jianwei Liu Xiaofeng Wang 《Journal of Plant Growth Regulation》2016,35(3):755-769
Brassinosteroid-insensitive 1 (BRI1), the receptor of brassinosteroids (BRs), is a dual-function serine/threonine/tyrosine protein kinase which initiates BR signaling and regulates plant growth via its protein kinase activity. Previous research has identified phosphorylation sites of Arabidopsis BRI1 in vivo and in vitro, but the significance of which to BR signaling and plant development has not been discussed comprehensively. To investigate this, we systematically characterized Arabidopsis BRI1 site-directed mutants in the weak bri1-5 background. For vegetative organ development regulation, we demonstrated that Thr-1039, Ser-1042, and Ser-1044 were critical for vegetative development because mutants with eliminated phosphorylation at these residues exhibited aberrant leaf growth, whereas Ser-1172 and Ser-1187 slightly inhibited leaf growth. For reproductive organ development regulation, first, the notion that Thr-1039, Ser-1042, and Ser-1044 were essential for normal plant height is supported by the evidence that mutations preventing phosphorylation at Thr-1039, Ser-1042, and Ser-1044 decreased plant height. Second, comparison of seed yield-related traits showed that unphosphorylated Ser-1168-Ala, Ser-1172-Ala, and Ser-1179-Ala+Thr-1180-Ala mutants reduced seed yield dramatically, whereas eliminating phosphorylation at Ser-1042 caused increased seed production. In addition, we found that Ser-1042 and Ser-1044 were essential for BR signaling. The unphosphorylated Ser-1042-Ala and Ser-1044-Ala mutants displayed hyposensitive phenotypes accompanied with decreased accumulation of dephosphorylated BRI1-EMS suppressor 1 (BES1) protein and increased Constitutive Photomorphogenesis Dwarf expression levels as well as limited inhibition of hypocotyl and root elongation under exogenous brassinolide. Taken together, our data suggest that BRI1 phosphorylation at specific sites differentially affects growth and development which may provide novel approaches to precisely regulate economic yield through modifying specific BRI1 phosphorylation sites in crop species. 相似文献
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Brassinosteroid biosynthesis and inactivation 总被引:5,自引:0,他引:5
Sunghwa Choe 《Physiologia plantarum》2006,126(4):539-548
The term brassinosteroids (BRs) refers to the growth-promoting plant steroidal hormones. Various developmental programs including but not limited to cell elongation, stress tolerance, and skoto-/photo-morphogenesis are controlled by subnanomolar concentrations of BRs. Accordingly, BR mutants that are defective in BR biosynthetic or signaling pathways usually display dwarfism. Characterization of numerous BR dwarf mutants isolated from Arabidopsis , pea, tomato, and rice greatly contributed to our understanding of BR biology. Recently, an enzyme that mediates the final step in the BR biosynthetic pathways has been characterized by two different groups. The brassinolide synthases (Cytochrome P450s 85A2 and 85A3) are multifunctional enzymes that catalyze the last three consecutive steps in BR biosynthetic pathways, namely, C-6 hydroxylation, dehydrogenation, and Baeyer-Villiger type oxidation. In addition, many of the previously unknown steps have been genetically characterized. This review aims to summarize the knowledge that has been developed during the last 2–3 years in this field of BR biosynthesis and inactivation research. 相似文献
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Brassinosteroid-insensitive dwarf mutants of Arabidopsis accumulate brassinosteroids 总被引:15,自引:0,他引:15
Noguchi T Fujioka S Choe S Takatsuto S Yoshida S Yuan H Feldmann KA Tax FE 《Plant physiology》1999,121(3):743-752
Seven dwarf mutants resembling brassinosteroid (BR)-biosynthetic dwarfs were isolated that did not respond significantly to the application of exogenous BRs. Genetic and molecular analyses revealed that these were novel alleles of BRI1 (Brassinosteroid-Insensitive 1), which encodes a receptor kinase that may act as a receptor for BRs or be involved in downstream signaling. The results of morphological and molecular analyses indicated that these represent a range of alleles from weak to null. The endogenous BRs were examined from 5-week-old plants of a null allele (bri1-4) and two weak alleles (bri1-5 and bri1-6). Previous analysis of endogenous BRs in several BR-biosynthetic dwarf mutants revealed that active BRs are deficient in these mutants. However, bri1-4 plants accumulated very high levels of brassinolide, castasterone, and typhasterol (57-, 128-, and 33-fold higher, respectively, than those of wild-type plants). Weaker alleles (bri1-5 and bri1-6) also accumulated considerable levels of brassinolide, castasterone, and typhasterol, but less than the null allele (bri1-4). The levels of 6-deoxoBRs in bri1 mutants were comparable to that of wild type. The accumulation of biologically active BRs may result from the inability to utilize these active BRs, the inability to regulate BR biosynthesis in bri1 mutants, or both. Therefore, BRI1 is required for the homeostasis of endogenous BR levels. 相似文献
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Keimei Oh Tadashi Matsumoto Ayumi Yamagami Atushi Ogawa Kazuhiro Yamada Ryuichiro Suzuki Takayuki Sawada Shozo Fujioka Yuko Yoshizawa Takeshi Nakano 《PloS one》2015,10(3)
Plant hormone brassinosteroids (BRs) are a group of polyhydroxylated steroids that play critical roles in regulating broad aspects of plant growth and development. The structural diversity of BRs is generated by the action of several groups of P450s. Brassinazole is a specific inhibitor of C-22 hydroxylase (CYP90B1) in BR biosynthesis, and the application use of brassinazole has emerged as an effective way of complementing BR-deficient mutants to elucidate the functions of BRs. In this article, we report a new triazole-type BR biosynthesis inhibitor, YCZ-18. Quantitative analysis the endogenous levels of BRs in Arabidopsis indicated that YCZ-18 significantly decreased the BR contents in plant tissues. Assessment of the binding affinity of YCZ-18to purified recombinant CYP90D1 indicated that YCZ-18 induced a typical type II binding spectrum with a Kd value of approximately 0.79 μM. Analysis of the mechanisms underlying the dwarf phenotype associated with YCZ-18 treatment of Arabidopsis indicated that the chemically induced dwarf phenotype was caused by a failure of cell elongation. Moreover, dissecting the effect of YCZ-18 on the induction or down regulation of genes responsive to BRs indicated that YCZ-18 regulated the expression of genes responsible for BRs deficiency in Arabidopsis. These findings indicate that YCZ-18 is a potent BR biosynthesis inhibitor and has a new target site, C23-hydroxylation in BR biosynthesis. Application of YCZ-18 will be a good starting point for further elucidation of the detailed mechanism of BR biosynthesis and its regulation. 相似文献
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Kim GT Fujioka S Kozuka T Tax FE Takatsuto S Yoshida S Tsukaya H 《The Plant journal : for cell and molecular biology》2005,41(5):710-721
Brassinosteroids (BRs) are plant hormones that are essential for a wide range of developmental processes in plants. Many of the genes responsible for the early reactions in the biosynthesis of BRs have recently been identified. However, several genes for enzymes that catalyze late steps in the biosynthesis pathways of BRs remain to be identified, and only a few genes responsible for the reactions that produce bioactive BRs have been identified. We found that the ROTUNDIFOLIA3 (ROT3) gene, encoding the enzyme CYP90C1, which was specifically involved in the regulation of leaf length in Arabidopsis thaliana, was required for the late steps in the BR biosynthesis pathway. ROT3 appears to be required for the conversion of typhasterol to castasterone, an activation step in the BR pathway. We also analyzed the gene most closely related to ROT3, CYP90D1, and found that double mutants for ROT3 and CYP90D1 had a severe dwarf phenotype, whereas cyp90d1 single knockout mutants did not. BR profiling in these mutants revealed that CYP90D1 was also involved in BR biosynthesis pathways. ROT3 and CYP90D1 were expressed differentially in leaves of A. thaliana, and the mutants for these two genes differed in their defects in elongation of hypocotyls under light conditions. The expression of CYP90D1 was strongly induced in leaf petioles in the dark. The results of the present study provide evidence that the two cytochrome P450s, CYP90C1 and CYP90D1, play distinct roles in organ-specific environmental regulation of the biosynthesis of BRs. 相似文献
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Junbo Du Hongju Yin Shasha Zhang Zhuoyun Wei Baolin Zhao Jinghua Zhang Xiaoping Gou Honghui Lin and Jia Li 《植物学报(英文版)》2012,54(6):388-399
Brassinosteroids(BRs),a group of plant steroidal hormones,play critical roles in many aspects of plant growth and development.Previous studies showed that BRI1-mediated BR signaling regulates cell division and differentiation during Arabidopsis root development via interplaying with auxin and other phytohormones.Arabidopsis somatic embryogenesis receptor-like kinases(SERKs),as co-receptors of BRI1,were found to play a fundamental role in an early activation step of BR signaling pathway.Here we report a novel function of SERKs in regulating Arabidopsis root development.Genetic analyses indicated that SERKs control root growth mainly via a BR-independent pathway.Although BR signaling pathway is completely disrupted in the serk1 bak1 bkk1 triple mutant,the root growth of the triple mutant is much severely damaged than the BR deficiency or signaling null mutants.More detailed analyses indicated that the triple mutant exhibited drastically reduced expression of a number of genes critical to polar auxin transport,cell cycle,endodermis development and root meristem differentiation,which were not observed in null BR biosynthesis mutant cpd and null BR signaling mutant bri1-701. 相似文献
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Kazuhiro Yamada Osamu Yajima Yuko Yoshizawa Keimei Oh 《Bioorganic & medicinal chemistry》2013,21(9):2451-2461
Brassinosteroids (BRs) are phytohormones that control several important agronomic traits, such as flowering, plant architecture, seed yield, and stress tolerance. To manipulate the BR levels in plant tissues using specific inhibitors of BR biosynthesis, a series of novel azole derivatives were synthesized and their inhibitory activity on BR biosynthesis was investigated. Structure–activity relationship studies revealed that 2RS, 4RS-1-[4-(2-allyloxyphenoxymethyl)-2-(4-chlorophenyl)-[1,3]dioxolan-2-ylmethyl]-1H-[1,2,4]triazole (G2) is a highly selective inhibitor of BR biosynthesis, with an IC50 value of approximately 46 ± 2 nM, which is the most potent BR biosynthesis inhibitor observed to date. Use of gibberellin (GA) biosynthesis mutants and BR signaling mutants to analyze the mechanism of action of this synthetic series indicated that the primary site of action is BR biosynthesis. Experiments feeding BR biosynthesis intermediates to chemically treated Arabidopsis seedlings suggested that the target sites of this synthetic series are CYP90s, which are responsible for the C-22 and/or C-23 hydroxylation of campesterol. 相似文献
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Recent advances in brassinosteroid molecular genetics 总被引:8,自引:0,他引:8
Altmann T 《Current opinion in plant biology》1998,1(5):378-383
The importance of brassinosteroids (BRs, a specific class of ecdysone-like plant steroids) as essential endogenous regulators of growth and development is demonstrated through a growing number of well characterised Arabidopsis, pea, and tomato mutants deficient in BR biosynthesis or BR response. Thus, a rapid advancement in understanding the molecular genetics of BR biosynthesis and mode of action can be witnessed, which will be further enhanced through the availability of a set of extremely valuable molecular tools for the analysis of the biological function of BRs. 相似文献
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Plants steroid hormones, brassinosteroids: current highlights of molecular aspects on their synthesis/metabolism, transport, perception and response 总被引:12,自引:0,他引:12
Brassinosteroids (BRs) are plant steroids essential for normal growth and development and can be defined as steroids that carry an oxygen moiety at C-3 and additional ones at one or more of the C-2, C-6, C-22 and C-23 carbon atoms. BR biosynthesis and metabolism mutants have been obtained and the corresponding genes cloned. These include genes encoding 5alpha-reductase and cytochrome P450 enzymes, that are similar to enzymes associated with mammalian steroid synthesis. Perception and/or response mutants have also been identified via screening for altered sensitivity to BRs. Some of these mutants have been found to be defective in a leucine-rich repeat receptor kinase and in a component of a vacuolar ATPase. This review highlights the recent advances in unraveling BR synthesis/metabolism, transport, perception and response through the analysis of BR mutants. 相似文献
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BIN2, a new brassinosteroid-insensitive locus in Arabidopsis 总被引:9,自引:0,他引:9
Brassinosteroids (BRs) play important roles throughout plant development. Although many genes have been identified that are involved in BR biosynthesis, genetic approaches in Arabidopsis have led to the identification of only one gene, BRI1, that encodes a membrane receptor for BRs. To expand our knowledge of the molecular mechanism(s) of plant steroid signaling, we analyzed many dwarf and semidwarf mutants collected from our previous genetic screens and identified a semidwarf mutant that showed little response to exogenous BR treatments. Genetic analysis of the bin2 (BR-INSENSITIVE 2) mutant indicated that the BR-insensitive dwarf phenotype was due to a semidominant mutation in the BIN2 gene that mapped to the middle of chromosome IV between the markers CH42 and AG. A direct screening for similar semidwarf mutants resulted in the identification of a second allele of the BIN2 gene. Despite some novel phenotypes observed with the bin2/+ mutants, the homozygous bin2 mutants were almost identical to the well-characterized bri1 mutants that are defective in BR perception. In addition to the BR-insensitive dwarf phenotype, bin2 mutants exhibited BR insensitivity when assayed for root growth inhibition and feedback inhibition of CPD gene expression. Furthermore, bin2 mutants displayed an abscisic acid-hypersensitive phenotype that is shared by the bri1 and BR-deficient mutants. A gene dosage experiment using triploid plants suggested that the bin2 phenotypes were likely caused by either neomorphic or hypermorphic gain-of-function mutations in the BIN2 gene. Thus, the two bin2 mutations define a novel genetic locus whose gene product might play a role in BR signaling. 相似文献
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The Regulation of Brassinosteroid Biosynthesis in Arabidopsis 总被引:1,自引:0,他引:1