苦瓜皂甙对衰老动物内分泌功能的影响

目的 :探讨苦瓜皂甙对衰老动物内分泌功能的调节作用。方法 :15月龄雌性老年昆明小鼠 ,随机分为老年对照组、实验 1组和实验 2组三组 ,同时准备 4月龄雌性昆明小鼠作青年对照组 ,其中两对照组饮水为普通水 ,两个实验组饮水分别含有 10 0mg/L及 2 0 0mg/L苦瓜皂甙 ,饲养 5周后取血清标本待测。同时 ,取 12月龄大鼠胸腺细胞培养 ,检测不同浓度苦瓜皂甙对雌激素受体蛋白表达的影响。结果 :与青年对照组相比 ,衰老小鼠血清ACTH、雌二醇水平显著降低。与老年对照组相比 ,两实验组血清雌二醇水平均明显升高 ,ACTH水平有升高趋势 ,但只有高剂量组有显著性 ;两组之间所有指标均没有显著性差异。体外实验发现 ,苦瓜皂甙明显促进雌激素受体蛋白的表达 ,却不影响其mRNA水平。结论 :苦瓜皂甙可通过调节ACTH分泌及雌激素受体表达来改善衰老机体内分泌功能     

The fetotoxic potential of municipal drinking water in the mouse.

Mice (CD-1 strain) were placed on diets containing either municipal drinking water (Durham, North Carolina) or water that had been distilled and passed through cartridges to reduce organics and remove inorganics. After a two-week acclimation period, animals were bred and pregnancy confirmed by the presence of a sperm plug. During the 8-month course of the study, approximately 500 pregnant mice were sacrificed on day 18 of gestation and their fetuses examined for visceral and skeletal anomalies. No significant water-related effects were found on any fetal parameter studied except for a 28.1% incidence of supernumerary ribs in the tap-water group as compared to 21.1% in the purified-water group. No differences were noted in the type or occurrence of anomalies between the two groups. A month to month variation was observed in a number of parameters. The degree of variation was similar for the treatment groups, suggesting that these changes might be random fluctuations.     

Monoclonal antibody production in murine ascites. I. Clinical and pathologic features

BACKGROUND AND PURPOSE: Murine ascites production has been associated with appreciable morbidity and mortality, thus raising animal-welfare concerns. To address these concerns, the clinicopathologic changes associated with in vivo production of monoclonal antibodies in mice were characterized, and results were compared among cell lines. METHODS: Five hybridoma cell lines were grown in groups of 20 mice. Fourteen days prior to inoculation with 10(6) hybridoma cells, mice were primed with 0.5 ml of pristane given intraperitoneally; 12 mice were sham treated (controls). Ascites fluid was collected a maximum of three times by abdominal paracentesis. Clinical observations and pre- and postabdominal tap body weights were recorded. Necropsies were performed on all mice. RESULTS: For all groups combined, overall survival to tap 1 was 98%, to tap 2 was 96%, and to tap 3 was 79%; survival among groups ranged from 90 to 100% for tap 1, 85 to 100% for tap 2, and 35 to 100% for tap 3. Disseminated intra-abdominal seeding with irregular soft tissue and/or solid tumor masses was observed at necropsy. CONCLUSIONS: Significant clinicopathologic changes were associated with monoclonal antibody production in mice, and differences between various hybridoma cell lines were apparent.     

Cytogenetic analysis of mouse metaphase II oocytes following exposure to tritiated water

Female mice were given different dosages (0, 3.0, 7.5, 15.0, or 30 muCi/ml) of tritium in their drinking water continuously from 3 to 7 weeks of age to assess the effects on germ cell chromosomes. At 8-9 weeks of age, mice were superovulated and metaphase II oocytes were processed and C-banded for cytogenetic analyses. Chromatid acentric fragments were the only type of structural aberration detected, and their incidence was higher in controls than in any of the tritiated water (HTO) groups. Analysis of numerical chromosomal aberrations revealed that the incidence of hypoploid (N = 19) oocytes was higher in oocytes from mice who drank HTO as compared with controls. However, the effects of HTO upon aneuploidy induction was not definitive due to the increase the incidence of aberrations in mouse oocytes can be related to the low dose rate resulting from chronic HTO exposure and possibly death of tritium-damaged cells.     

Interactions between sodium and calcium intake and blood pressure responses to norepinephrine and parathyroid hormone

The present study has examined the vascular responses to infusion of norepinephrine (NE) and parathyroid hormone (PTH 1-34) in animals subjected for a six month period to one of four dietary regimens. Some animals received normal calcium chow (1.0% Ca by weight) and drank water (subgroup A), others consumed the same chow, but water was replaced by 0.5% saline (subgroup B), a third group consumed chow which had 2.0% Ca content and also drank 0.5% saline (subgroup C) and a fourth group consumed the 2.0% Ca chow, but drank water (subgroup D). No differences were found in the pressor response to NE across subgroup A, B, and C, while pressor response to NE in subgroup D was markedly reduced. Depressor responses to PTH were not significantly different across any of the four groups. The ability of changes in calcium homeostasis to affect blood pressure responses to NE and PTH were evaluated in animals consuming reduced dietary calcium (0.1%) for two and four weeks and compared with animals on normal calcium intake (1.0%). This dietary treatment resulted in only mild effects on calcium balance; after four weeks no significant difference in plasma total calcium concentration was observed, but plasma PTH levels were increased in animals on the low Ca diet. No effects on the blood pressure response to NE or PTH infusion were observed after 2 weeks of dietary treatment. At four weeks, NE responses remained unchanged, while responses to PTH were blunted in animals on 0.1% Ca chow.(ABSTRACT TRUNCATED AT 250 WORDS)     

Embryo transfer in two-year-old donor mares

Embryos were collected from two-year-old donor mares and transferred surgically during 1983 and 1984. The overall embryo recovery rate from two-year-old donors was 36.3%. Over both years, 71.4% of the donors ($\text{30}\text{42}$) provided at least one embryo. There was a trend both years for slightly higher embryo recovery rates prior to August 1 (47.7%), as compared to after August 1 (31.1%). Pregnancy rates in recipient mares after surgical embryo transfer were not affected by month of the breeding season. However, there was a trend for improved pregnancy rates when embryos were transferred after August 1 (87.5%), as compared to before August 1 (70.0%). There was a 8.3% incidence of fetal loss between Days 15 and 35 of gestation in recipients. The incidence of fetal loss between Days 35 and 60 of gestation was 2.7%. Based on these data, the chance of obtaining a pregnancy from a two-year-old mare is 28.2% (36.3% recovery × 77.7% pregnancy rate). Thus embryo transfer may prove to be a beneficial tool for obtaining foals from two-year-old donor mares.     

子宫内膜异位症合并不孕的原因及治疗方案探讨

目的：探讨子宫内膜异位症并不孕的原因及治疗方案的临床意义。方法：随机选取2007年6月至2012年8月就诊于我院的不孕症或疑似子宫内膜异位症（EMS）经检查确诊为EMS的患者216例,按照用药区别平均分为六组,分别为三苯氧胺组、米非司酮组、丹那唑组、诺雷德组、无效成分安慰剂组及两阶段复合治疗组,记录上述患者经治疗后随访1年内受孕率情况。结果：无效成分安慰剂组无疗效,各方面与其他组比较有明显差异（P〈0.05）,其余各组疗效相比无明显差异（P〉0.05）;三苯氧胺组与丹那唑组不良反应发生率高于其他组,比较有明显差异（P〈0.05）;两阶段复合治疗组的复发率最低,受孕率最高,除安慰剂组外其他组之间比较无明显差异（P〉0.05）。结论：此次研究的药物治疗效果近似,但是副作用、复发率及受孕率具有差异,临床应根据患者具体情况施治。     

Sire effect on the pregnancy outcome in beef cows synchronized with progesterone based Ovsynch and CO-Synch protocols

The objective was to determine the sire effect on the pregnancy outcome in beef cows in which stage of estrus was synchronized with progesterone based fixed-time artificial insemination (AI) protocols. Three Angus sires with more than 300 breedings were evaluated for differences in pregnancy outcome from 1868 inseminations. Angus cross beef cows (N=1868) were synchronized with Ovsynch-CIDR or CO-Synch-CIDR protocols for fixed-time AI. Cows in both groups that showed estrus on day 9 before 1500 h were designated to Selectsynch-CIDR group and were inseminated according to AM-PM rule. Results indicated that Sire 2 had lower fixed-time AI pregnancy rate compared to Sire 3 (48.1% versus 58.7%; P=0.01). Significant sirexsynchronization program and sirexlocation interactions were observed for fixed-time AI (P<0.05). Sire 2 had a lesser fixed-time AI pregnancy in both Ovsynch-CIDR and CO-Synch-CIDR groups compared to Sire 3. In two of four locations, Sire 2 had a lesser fixed-time pregnancy rate compared to Sire 3. No sire differences were observed in AI pregnancy for cows in Selectsynch-CIDR group. In conclusion, evidence in this study suggest that there are differences in sire fertility when they were used in fixed-time AI protocols, possibly due to the sire differences in sperm capacitation process. Further studies are needed to investigate association of the sire differences in fixed-time AI protocols with sire differences in the sperm capacitation process.     

饮用经臭氧处理的灭菌水与小鼠自发性乳腺肿瘤的发生

目的研究饮用经臭氧处理的灭菌水对小鼠自发性乳腺肿瘤发生的影响。方法给昆明(KM)小鼠、NIH小鼠和BALB/c小鼠饮用经臭氧处理的灭菌水,然后观察其乳腺肿瘤的发生情况,剖检小鼠并从其乳腺、左右肺叶、气管、肺门淋巴结、鼠蹊部淋巴结、心、肝、脾、肾、脑和大小肠等部位取材固定,常规病理制片进行病理组织学检查。结果饮用经臭氧处理的水数月后,KM小鼠、NIH小鼠和BALB/c小鼠,自发性乳腺肿瘤的数量明显增加。发生的乳腺肿瘤均为乳腺腺癌,其中一例为乳头状囊腺癌。乳腺肿瘤均发生在生育3～4胎以上的雌性小鼠。9例中有3例发生肺转移癌。根据乳腺肿瘤发生部位、大体形态特征及病理组织学,结合临床发病情况,即可明确诊断。结论长期饮用经臭氧处理的灭菌水可使小鼠自发性乳腺肿瘤的发生率明显上升。     

The efficacy of different hCG dose rates and the effect of hCG treatment on ovarian activity: ovulation, multiple ovulation, pregnancy, multiple pregnancy, synchrony of multiple ovulation; in the mare

Despite the widespread use of hCG to advance ovulation in the mare there is little information on efficacy of dose rates and any contraindications of its use. This study aims to investigate the effect of dose of hCG on ovulation within 48h and the effect of hCG on: ovulation, multiple ovulation (MO), pregnancy, multiple pregnancy (MP) rates and synchrony of MO; additionally whether any seasonal effect is evident. Sequential ultrasonic scanning was used to monitor the occurrence of ovulation, within 48h of treatment, in 1291 Thoroughbred mares treated with either 750iu hCG or 1500iu hCG s.c. Ovulation rate, type (single ovulations (SO), MO, synchronous, asynchronous) and subsequent pregnancy were then monitored in 1239 Thoroughbred mares on a commercial stud over 3 years, 536 of which were treated with 750iu hCG at mating, all mares were also allocated into groups according to month of mating. No significant difference existed between the two dose levels of hCG and no significant difference existed between treated and untreated mares in overall ovulations (1.32 and 1.28 respectively), MO (31.7% and 27.7%), pregnancy (65.1% and 65.6%) or MP rates (10.8% and 11.8%). There was no significant association between month of year and pregnancy or MP rates for either treated or control mares, nor for MO for untreated mares. A significant (p<0.05) association was evident between month and MO in treated mares, MO being lowest in April (22.3%). 95.9% of treated mares multiple ovulated within 48h compared with 90.7% controls, a near significant difference. In conclusion this study demonstrates that: (i) hCG dose of 750iu s.c. is just as effective in inducing ovulation within 48h as 1500iu, (ii) 750iu hCG has no significant effect on ovulation, MO, pregnancy or MP rates; (iii) a significant (p<0.05) association exists between season and MO in hCG treated mares; (iv) a tighter synchrony (ovulation within 48h) of MO is evident in hCG treated compared with control mares (p=0.052).     

Reproductive performance of dairy cows with ovarian cysts after synchronizing ovulation using GnRH or hCG during the warm or cool period of the year

This study was designed to compare the reproductive response to timed AI of lactating dairy cows with cystic ovarian follicles treated with GnRH or hCG to synchronize ovulation. The effectiveness of treatment during the warm or cool period of the year was also compared. Cows were given 12 microg GnRH-agonist i.m. on day 0 of the protocol, 15 mg PGF(2alpha) i.m. on day 7, and either GnRH-agonist (GPG treatment) or 3000 IU hCG i.m. (GPH treatment) on day 9, followed by timed AI. The cows were randomly chronologically assigned to GPG (n=130) or GPH (n=136) group. All cows were inseminated at fixed time 16-22 h after the end of treatment. During the warm period the pregnancy rate to first AI was 12% (7/60) and 21% (14/68) for the GPG and GPH groups, respectively, there being no significant differences between groups; the cumulative pregnancy rate was 22% (13/60) and 21% (14/68) for the GPG and GPH groups, respectively, again with no significant intergroup differences. During the cool period pregnancy rate to first AI was not different between groups: 29% (20/70) for GPG and 32% (22/68) for GPH, respectively; whereas the cumulative pregnancy rate was significantly higher (P<0.05) for the GPH groups than for the GPG group: 56% (39/70) and 78% (53/68), respectively. These findings indicate that during the warm period, the pregnancy rates of the cystic cows were similar whether they received GPG or GPH treatment, during the cool period, there is a beneficial effect to use hCG at day 9 of the ovsynch protocol compared GnRH on cumulative pregnancy rate.     

Chronic ethanol consumption in mice does not induce DNA damage in somatic or germ cells, evaluated by the bone marrow micronucleous assay and the dominant lethal mutation assay

Although alcohol is known to be a carcinogen for humans, ethanol-genotoxicity studies are incomplete. Ethanol seems not to be a bacterial mutagen, but the results are conflicting in rodent assays. We investigate the genotoxicity in the bone marrow micronucleus (MN) test and in the dominant lethal mutation (DLM) assay using two long-term ethanol exposure protocols. In the MN test, mice consumed three doses (5, 10 and 15% v/v) for 32 weeks. MN induction was compared to two control groups of 5- and 38-week-old mice (the ages of the treated mice when the treatment was initiated and when they were killed, respectively). For the three groups treated with ethanol there was no significant increase in MN induction as compared to the first control group, but observed MN frequencies were significantly lower than in the 38-week-old control group. This suggests a protective effect against genotoxic damage caused by aging, probably due to ethanol action as a hydroxyl radical scavenger. In the DLM assay, male mice drank ethanol at 15% or 30% (v/v) for 20 weeks. In both groups the number of dead implants was similar to the control, but there was a significant reduction in total implants, indicating a pre-implantation loss.     

Plasma angiotensin II: interdependence on sodium and calcium homeostasis

Interactions between sodium and calcium metabolism and the renin angiotensin system (RAS) have been studied. In rats drinking highly palatable 0.5% sodium chloride solution for a 6 month period, plasma angiotensin II (p[AII]) levels after 6 months did not differ from control animals drinking water. However, plasma ionized calcium (p[iCa]) levels were significantly reduced compared to controls. In a third group of animals which drank saline, but consumed a calcium supplemented chow (2% calcium by weight vs. 1%), p[AII] was significantly elevated above both other groups. Further experiments were performed to study short term (4 weeks) changes in calcium intake and p[AII] levels. Diets contained high (4%), normal (1%) and low (0.05%) calcium content. All animals drank water. Plasma total calcium (p[tCa]) and p[iCa] concentration were elevated in the 4% calcium group compared with 1% calcium. In the 0.05% calcium group, p[iCa] was significantly reduced compared with the 1% group. Compared with the 1% calcium group, 4% calcium animals showed significant elevation of p[AII] levels. A slight, insignificant elevation was observed in 0.05% calcium rats compared with those consuming 1% calcium. A final experiment studied animals on the same calcium intakes (0.05, 1 and 4%), but consuming 0.5% saline in place of water. No differences in p[iCa], p[tCa] or p[AII] were observed in these experiments. However, consumption of saline lead to the expected reduction in p[AII] levels which was absent after 6 months in the earlier studies, indicating that normal levels of p[AII] in saline drinkers after 6 months was not a measurement error.(ABSTRACT TRUNCATED AT 250 WORDS)     

水体中病毒浓缩方法及其条件优化

本研究探讨了在自来水和污水两种不同的水体环境中, 三氯化铝沉淀法和正电荷滤膜法浓缩回收病毒的效率, 并比较应用不同的浓缩条件、洗脱物质时的病毒回收率, 从而建立有效的环境样本中病毒的浓缩方法。结果表明, 三氯化铝沉淀法在两种水体中的回收率均较高, 最高回收率分别达到96.0%和92.0%, 但正电荷滤膜法在两种水体中的回收率差别较大。在自来水中, 正电荷滤膜法的最高回收率为93.9%, 在污水中的最高回收率仅为69.9%, 这说明正电荷滤膜法适用于病毒含量较高且悬浮物等杂质较少的样本。在应用于自来水样时, 两种方法均可起到有效的病毒浓缩作用, 但在悬浮物等杂质较多的污水中, 三氯化铝法具有较好的回收效率。     

超声乳化白内障吸除、人工晶状体植入联合小梁切除术治疗白内障合并青光眼的临床疗效及安全性评价

目的：探讨超声乳化白内障吸除、人工晶状体植入联合小梁切除术治疗白内障合并青光眼的临床疗效及安全性。方法：将100例白内障合并青光眼患者按照抽签法随机地均分为对照组与观察组,对照组给予单纯超声乳化人工晶状体植入术,观察组在此基础上给予联合小梁切除术进行治疗。比较两组治疗前后相关指标以及术后并发症发生率等。结果：（1）对照组治疗前后IOP、ACD及AL均无统计学差异,观察组治疗前后IOP与CAD差异具有统计学意义（P〈0．05,P〈0．01）,但该组治疗前后AL无统计学差异;（2）两组术前与术后1周、1个月及3个月平均视野缺损值、平均模式标准差相比,差异均具有统计学意义,且观察组术后与对照组术后相比,差异均具有统计学意义;（3）对照组并发症发生率为20．00％,明显大于观察组（10．00％）。结论：超声乳化白内障吸除、人工晶状体植入联合小梁切除术治疗白内障合并青光眼,疗效显著,不良反应发生率低,值得加以推广并应用。     

Twinning in mares: A survey of veterinarians and analyses of theriogenology records

Responses of 22 veterinarians to a questionnaire and the records of three brood-mare farms were examined to obtain information on twinning. The incidence rates of multiple ovulations on the three farms were 9%, 11%, and 22%. The multiple ovulation rate was reduced 42-67% in foaling mares compared to barren and maiden mares. Multiple ovulations were significantly more frequent in Thoroughbreds (19%) than in Quarter Horses (9%) and Appaloosas (8%). Both the questionnaire and the farm records indicated a high degree of repeatability of multiple ovulations and twin pregnancies within mares and within certain family lines. The likelihood of multiple ovulations was approximately doubled when the preceding cycle also had multiple ovulations. On two of the farms, breeding was withheld or postponed until after the first ovulation when multiple follicles were present. There were no significant differences in overall reproductive efficiency (pregnancy and foaling rates) among the three farms and the incidence of observed twin abortions or births was low on all three farms (0.4, 0.2, and 0%, respectively).     

The influence of some nutritional factors on the incidence of the repeat breeder syndrome in high producing dairy herds

Data on feeding and the incidence of reproduction disorders in dairy cattle were collected and compared between 2 groups composed of 6 Kibutz dairy herds each. One group contained herds picked at random from dairies with a good overall conception rate (45% and above according to rectal pregnancy examinations) and the other group was randomly selected from those with a low overall conception rate (32% and below).The two groups differed significantly in the incidences of repeat breeder cows (5.48% and 27.35%, respectively), the percentage of problem cows not pregnant 150 days after parturition (14.62% and 38.8%), days to conception (98.5 and 113.7 days), percentage of cows culled due to infertility (5.6% and 15.33%), and in the feeding practices.During the first half of lactation (the high production period) the differences occurred in the amount of digestible protein (11.9% and 9.9% of total dry matter) and in the amount of roughage (33.5% and 20.3% of total dry matter) fed. During the second half of lactation the significant differences between the 2 groups were in the energy level (16.5 F.U. and 13.6 F.U.) and digestible protein fed (10.25% and 8.73% of total dry matter), as well as in the total dry matter fed (17.7 Kg and 15.1 Kg). In the dry period, the only significant difference was in the total dry matter fed (12.7 Kg and 10.5 Kg).It appears that the incidence of repeat breeder cows is influenced by nutritional factors, particularly by the roughage, protein, and energy levels. Proper amounts of these three constituents in the ration during the entire inter-valving period is essential for providing appropriate fertility levels in high producing dairy herds.     

Water movements after an intraruminal water load in pregnant and lactating Sardi sheep

The effects of an intraruminal load of 3 litres of water on body water movements was compared in Sardi sheep during the last month of pregnancy, lactation and a non-pregnant, non-lactating control period. Before the water load, rumen fluid volume, estimated by polyethylene glycol was similar in pregnant, compared to control, animals and 27% higher in lactating sheep. After the water load, rumen volume returned to pre-hydration level in 1 h during pregnancy, after 3 h during lactation and in the control period. Rumen osmolality decreased by 40% and remained at this low level for 3 h after the water load in all physiological periods. When the water load was tritiated water (TOH), the rate of TOH transfer into plasma was faster during the last month of pregnancy than during the control period. Plasma osmolality and proteins decreased in response to the water load. No differences in these responses were observed between pregnancy, lactation and the control period. Water diuresis began in the first 30 min following the water load in pregnant ewes and in the second 30 min in lactating and control ewes. The diuresis was also more pronounced in pregnant, than in non-pregnant, states. These results indicate that water is more rapidly absorbed from the gastrointestinal tract in pregnant, rather than in non-pregnant, sheep. This may partly explain the increased water turnover seen during pregnancy.     

Chronic exposure of cancer-prone mice to low-level 2450 MHz radiofrequency radiation

The purpose of this study was to determine whether chronic, low-level exposure of mammary-tumor-prone mice to 2450 MHz radiofrequency radiation (RFR) promotes an earlier onset (decreased latency), a greater total incidence, or a faster growth rate of mammary tumors. One hundred C3H/HeJ mice were exposed in circularly polarized waveguides (CWG) for 18 months (20 h/day, 7 days/wk) to continuous-wave, 2450 MHz RFR at a whole body average specific absorption rate (SAR) of 0.3 W/kg; 100 mice were sham exposed. Before exposure, SARs were determined calorimetrically; during experimentation, SARs were monitored by differential power measurement. All animals were visually inspected twice daily and were removed from the CWG cages for a weekly inspection, palpation, and weighing. From the time of detection, tumor size was measured weekly. Animals that died spontaneously, became moribund, or were killed after 18 months of exposure were completely necropsied; tissues were fixed and subjected to histopathological evaluations. Results showed no significant difference in weight profiles between sham-irradiated and irradiated mice. Concerning mammary carcinomas, there was no significant difference between groups with respect to palpated tumor incidence (sham = 52%; irradiated = 44%), latency to tumor onset (sham = 62.3 ± 1.2 wk; irradiated = 64.0 ± 1.6 wk), and rate of tumor growth. In general, histopathological examination revealed no significant differences in numbers of malignant, metastatic, or benign neoplasms between the two groups; a significantly greater incidence of alveolar-bronchiolar adenoma in the sham-irradiated mice was the only exception. In addition, survival analysis showed no significant difference in cumulative percent survival between sham and irradiated animals. Thus, results indicate that under the conditions of this study, long-term, low-level exposure of mammary-tumor-prone mice to 2450 MHz RFR did not affect mammary tumor incidence, latency to tumor onset, tumor growth rate, or animal longevity when compared with sham-irradiated controls. Bioelectromagnetics 19:20–31, 1998. © 1998 Wiley-Liss, Inc.     

2月龄和4月龄大鼠制备心肺复苏模型后神经功能评价及亚组评分的比较

目的：本研究旨在选择适合的大鼠,对比2月龄大鼠与4月龄大鼠CPR后神经功能评分及亚组评分,摸索利用不同月龄的大鼠CPR的可行性。方法通过严格监测SD大鼠制备模型过程中的心电、血压生理指标、测定各组大鼠不同时间点的神经功能缺失评分以及神经功能亚组评分、对比制备4月龄和2月龄大鼠心肺复苏模型的稳定性。结果电刺激致室颤,4月龄组大鼠的成模率为87.5％,远高于2月龄组大鼠,而死亡率无差异;在电刺激诱发CA过程中造成的血压变化,4月龄组大鼠明显低于2月龄组大鼠,存在极显著性差异（ P ＜0.01）;4月龄组大鼠与2月龄组大鼠CPR后各时间点的神经功能评分无统计学差异,而4月龄组大鼠与2月龄组大鼠的神经功能亚组评分在不同时间点,存在显著差异（P ＜0.05）,4月龄大鼠与2月龄大鼠对比,心肺复苏后脑损伤的程度加重。结论4月龄大鼠更适合制备心肺复苏模型,该月龄组的大鼠制备模型成模率高,脑损伤程度重,更适合于用于心肺复苏的基础研究及治疗评价。