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1.
Containment of a genetically engineered microorganism during a field bioremediation application 总被引:6,自引:0,他引:6
A field release of a genetically engineered microorganism was performed at the Field Lysimeter Site on the Oak Ridge Reservation.
Six large lysimeters were filled with soil that had been contaminated with a mixture of naphthalene, phenanthrene, and anthracene.
A genetically engineered bacterial strain, Pseudomonas fluorescens HK44, was sprayed onto the surface of the soil during soil loading. This strain contains a fusion between the lux genes of Vibrio fischeri and the promoter for the lower pathway of naphthalene degradation, enabling the strain to become bioluminescent when it is
degrading naphthalene. Release of the bacteria outside the lysimeters was monitored, using selective agar plates and one-stage
Anderson air samplers. Although approximately 1014 bacteria were sprayed during the loading process, escape was only detected sporadically; the highest incidence of bacterial
escape was found when the relative humidity and wind speed were low.
Received: 6 March 1998 /thinsp;Received revision: 16 September 1998 / Accepted: 16 October 1998 相似文献
2.
G. Zellner E. Feuerhake H. J. Jördening A. J. L. Macario E. Conway de Macario 《Applied microbiology and biotechnology》1995,43(3):566-571
A denitrifying bacterial biofilm population established on a polypropylene substratum of a fixed-film reactor was characterized
by microscopy, scanning electron microscopy and immunofluorescence after 120 days of operation. The reactor, operated at pH
7.0, 22°C, and −180 mV with synthetic wastewater containing methanol/nitrate, achieved a denitrification rate of 0.24 mol
NO-
3 l-1 day-1 with a removal efficiency for nitrate of 95%–99% at an organic loading rate of 0.325 mol methanol l-1 day-1. The gas produced contained 2%–3% (v/v) methane and 3%–4% (v/v) carbon dioxide in addition to nitrogen. The biofilm contained
mainly cells of Methanobrevibacter arboriphilus antigenically related to strain DC, short, flagellated, gram-negatively staining rods of Pseudomonas sp. antigenically related to Pseudomonas stutzeri strain AN11, non-identified pink-pigmented rods and small lemon-shaped cells with mono- and bipolar appendages resembling
prosthecate Hyphomicrobium sp. The biofilm analysis provided evidence for a syntrophy between the denitrifying, methylotrophic, bacterial consortium
and hydrogenotrophic methanogens, which were identified by antigenic fingerprinting with 17 antibody probes.
Received: 11 July 1994/Received revision: 23 September 1994/Accepted: 28 September 1994 相似文献
3.
A. E. Filonov W. A. Duetz A. V. Karpov R. R. Gaiazov I. A. Kosheleva A. M. Breure I. F. Filonova J. G. van Andel A. M. Boronin 《Applied microbiology and biotechnology》1997,48(4):493-498
Plasmid-carrying Pseudomonas putida strains degrade naphthalene through different biochemical pathways. The influence of various combinations of host bacteria
and plasmids on growth characteristics and competitiveness of P. putida strains was studied in chemostat culture at a low dilution rate (D=0.05 h−1) with naphthalene as the sole source of carbon and energy. Under naphthalene limitation, the plasmid-bearing strains degrading
naphthalene that use catechol 1,2-dioxygenase for catechol oxidation (ortho pathway), were the most competitive. The strains bearing plasmids that control naphthalene catabolism via catechol 2,3-dioxygenase
(meta pathway), were less competitive. Under these conditions the strain carrying plasmid pBS4, which encodes for naphthalene catabolism
via gentisic acid, was the least competitive.
Received: 24 February 1997 / Received revision: 22 May 1997 / Accepted: 25 May 1997 相似文献
4.
The Aspergillus niger strain BO-1 was grown in batch, continuous (chemostat) and fed-batch cultivations in order to study the production of the
extracellular enzyme glucoamylase under different growth conditions. In the pH range 2.5–6.0, the specific glucoamylase productivity
and the specific growth rate of the fungus were independent of pH when grown in batch cultivations. The specific glucoamylase
producivity increased linearly with the specific growth rate in the range 0–0.1 h−1 and was constant in the range 0.1–0.2 h−1. Maltose and maltodextrin were non-inducing carbon sources compared to glucose, and the maximum specific growth rate was
0.19 ± 0.02 h−1 irrespective of whether glucose or maltose was the carbon source. In fed-batch cultivations, glucoamylase titres of up to
6.5 g l−1 were obtained even though the strain contained only one copy of the glaA gene.
Received: 5 May 1999 / Received revision: 7 September 1999 / Accepted: 17 September 1999 相似文献
5.
Poly(hydroxybutyric acid) (PHB) was produced by a selectant of Azotobacter beijerinckii in media containing only organic nitrogen sources such as N substrates. The chosen compounds were casein peptone, yeast extract,
casamino acids and urea, each combined with carbon substrates glucose or sucrose. The PHB was synthesized under growth-associated
conditions. The concentrations amounted to more than 50% of cell dry mass on casein peptone/glucose as well as urea/glucose
medium within 45 h fermentation time. Corresponding to these yields, productivities of about 0.8 g PHB l−1 h−1 were discovered. The highest values increased to 1.06 g PHB l−1 h−1 on casein peptone/glucose medium and 1.1 g PHB l−1 h−1 on yeast extract/glucose medium after a period of 20 h. It was found that oxygen limitation was essential for successful
product formation, as demonstrated earlier. These data from basic research may support further investigations into the use
of technical proteins from renewable sources as substrates for PHB production by a strain of A. beijerinckii.
Received: 3 June 1997 / Received revision: 29 August 1997 / Accepted: 15 September 1997 相似文献
6.
Potential for biodegradation of hydrocarbons in soil from the Ross Dependency, Antarctica 总被引:13,自引:0,他引:13
Oil spills occur in the Antarctic when fuel oils such as JP8 jet fuel are moved or stored. Hydrocarbons, both n-alkanes and aromatic compounds, have been detected in oil-contaminated soils of the Ross Dependency. In such areas hydrocarbon-degrading
microbes, if naturally occurring, could be used for clean-up. Soil samples from oil-impacted and control sites were analysed
for hydrocarbon-degrading microbes and for a range of parameters known to limit biodegradative activity. Soils were analysed
for water content, pH, concentrations of nutrients (N and P) and electrical conductivity. Numbers of culturable heterotrophic
bacteria and hydrocarbon degraders were greater in some of the oil-contaminated samples. Mineralisation studies with 14C-radiolabelled hexadecane and naphthalene demonstrated that nitrogen amendments significantly enhanced hydrocarbon mineralisation
rates in an oil-impacted soil.
Received: 30 June 1997 / Received revision: 15 September 1997 / Accepted: 19 September 1997 相似文献
7.
The ability of Alcaligenes eutrophus to grow and produce polyhydroxyalkanoates (PHA) on plant oils was evaluated. When olive oil, corn oil, or palm oil was fed
as a sole carbon source, the wild-type strain of A. eutrophus grew well and accumulated poly(3-hydroxybutyrate) homopolymer up to approximately 80% (w/w) of the cell dry weight during
its stationary growth phase. In addition, a recombinant strain of A. eutrophus PHB−4 (a PHA-negative mutant), harboring a PHA synthase gene from Aeromonas caviae, was revealed to produce a random copolyester of 3-hydroxybutyrate and 3-hydroxyhexanoate from these plant oils with a high
cellular content (approximately 80% w/w). The mole fraction of 3-hydroxyhexanoate units was 4–5 mol% whatever the structure
of the triglycerides fed. The polyesters produced by the A. eutrophus strains from olive oil were 200–400 kDa (the number-average molecular mass). The results demonstrate that renewable and inexpensive
plant oils are excellent carbon sources for efficient production of PHA using A. eutrophus strains.
Received: 3 September 1997 / Received revision: 10 November 1997 / Accepted: 16 November 1997 相似文献
8.
Yanase H Sakamoto A Okamoto K Kita K Sato Y 《Applied microbiology and biotechnology》2000,53(3):328-334
A fungus with the ability to utilize a metal-cyano compound, tetracyanonickelate (II) {K2[Ni (CN)4]; TCN}, as its sole source of nitrogen was isolated from soil and identified as Fusarium oxysporum N-10. Both intact mycelia and cell-free extract of the strain catalyzed hydrolysis of TCN to formate and ammonia and produced
formamide as an intermediate, thereby indicating that a hydratase and an amidase sequentially participated in the degradation
of TCN. The enzyme catalyzing the hydration of TCN was purified approximately ten-fold from the cell-free extract of strain
N-10 with a yield of 29%. The molecular mass of the active enzyme was estimated to be 160 kDa. The enzyme appears to exist
as a homotetramer, each subunit having a molecular mass of 40 kDa. The enzyme also catalyzed the hydration of KCN, with a
cyanide-hydrating activity 2 × 104 times greater than for TCN. The kinetic parameters for TCN and KCN indicated that hydratase isolated from F. oxysporum was a cyanide hydratase able to utilize a broad range of cyano compounds and nitriles as substrates.
Received: 9 August 1999 / Received revision: 13 September 1999 / Accepted: 24 September 1999 相似文献
9.
J. Ramón De Lucas Susana Valenciano Fernando Laborda Geoffrey Turner 《Archives of microbiology》1994,162(6):409-413
The existence of a second mechanism of catabolite control of isocitrate lyase of Aspergillus nidulans, in addition to the carbon catabolite repression phenomenon recently reported was analysed. Isocitrate lyase was rapidly
and specifically inactivated by glucose. The inactivation was irreversible at all stages in the presence of cycloheximide,
showing that reactivation depends on de novo protein synthesis. In addition, analysis of glucose-induced inactivation of isocitrate
lyase in a creA
d
-30 strain showed that the creA gene is not involved in this process.
Received: 13 May 1994 / Accepted 12 August 1994 相似文献
10.
A Pseudomonas sp. strain NGK 1 (NCIM 5120) was immobilized in various matrices, namely, alginate, agar (1.8 × 1011 cfu g−1 beads) and polyacrylamide (1.6 × 1011 cfu g−1 beads). The degradation of naphthalene was studied, by freely suspended cells (4 × 1010 cfu ml−1) and immobilized cells in batches, with shaken culture and continuous degradation in a packed-bed reactor. Free cells brought
about the complete degradation of 25 mmol naphthalene after 3 days of incubation, whereas, a maximum of 30 mmol naphthalene
was degraded by the bacteria after 3–4 days of incubation with 50 mmol and 75 mmol naphthalene, and no further degradation
was observed even after 15 days of incubation. Alginate-entrapped cells had degraded 25 mmol naphthalene after 3.5 days of
incubation, whereas agar- and polyacrylamide-entrapped cells took 2.5 days; 50 mmol naphthalene was completely degraded by
the immobilized cells after 6–7 days of incubation. Maximum amounts of 55 mmol, 70 mmol and 67 mmol naphthalene were degraded,
from an initial 75 mmol naphthalene, by the alginate-, agar- and polyacrylamide-entrapped cells after 15 days of incubation.
When the cell concentrations were doubled, 25 mmol and 50 mmol naphthalene were degraded after 2 and 5.5 days of incubation
by the immobilized cells. Complete degradation of 75 mmol naphthalene occurred after 10 days incubation with agar- and polyacrylamide-entrapped␣cells,
whereas only 60 mmol naphthalene was degraded by alginate-entrapped cells after 15 days of␣incubation. Further, with 25 mmol
naphthalene, alginate-, agar- and polyacrylamide-entrapped cells (1.8 × 1011 cfu g−1 beads) could be reused 18, 12 and 23 times respectively. During continuous degradation in a packed-bed reactor, 80 mmol naphthalene
100 ml−1 h−1 was degraded by alginate- and polyacrylamide-entrapped cells whereas 80 mmol naphthalene 125 ml−1␣h−1 was degraded by agar-entrapped cells.
Received: 21 October 1997 / Received revision: 15 January 1998 / Accepted: 18 January 1998 相似文献
11.
M. Graf A. Brunella M. Kittelmann K. Laumen O. Ghisalba 《Applied microbiology and biotechnology》1997,47(6):650-657
A new amidohydrolase deacetylating several N-acetyl-1-phenylethylamine derivatives (R)-specifically was found in Arthrobacter aurescens AcR5b. The strain was isolated from a wet haystack by enrichment culture with (R)-N-acetyl-1-phenylethylamine as the sole carbon source. (R) and (S )-N-acetyl-1-phenylethylamine do not serve as inducers for acylase formation. By improving the growth conditions the enzyme production
was increased 47-fold. The amidohydrolase was purified to homogeneity leading to a 5.2-fold increase of the specific activity
with a recovery of 67%. A molecular mass of 220 kDa was estimated by gel filtration. Sodium dodecyl sulfate/polyacrylamide
gel electrophorosis shows two subunits with molecular masses of 16 kDa and 89 kDa. The optimum pH and temperature were pH 8
and 50 °C, respectively. The enzyme was stable in the range of pH 7–9 and at temperatures up to 30 °C. The enzyme activity
was inhibited by Cu2+, Co2+, Ni2+, and Zn2+, and this inhibition was reversed by EDTA.M
Received: 20 September 1996 / Received version: 23 December 1996 / Accepted: 30 December 1996 相似文献
12.
Isolation and characterization of a 2-methylphenanthrene utilizing bacterium: identification of ring cleavage metabolites 总被引:1,自引:0,他引:1
J. Sabaté M. Grifoll M. Viñas A. M. Solanas 《Applied microbiology and biotechnology》1999,52(5):704-712
A bacterial strain capable of utilizing 2-methylphenanthrene (2-MP) as its sole source of carbon and energy for growth was
isolated from creosote contaminated soil. The isolate was identified as a strain of Sphingomonas sp. and was designated strain JS5. Utilization of 2-MP by strain JS5 was demonstrated by an increase in bacterial biomass
concomitant with a decrease of 2-MP in liquid mineral medium with this compound as sole source of carbon and energy. Growth
yield indicated a 23% assimilation of 2-MP carbon. Washed-cell suspensions of strain JS5 incubated with 2-MP accumulated a
major metabolite identified as 1-hydroxy-6-methyl-2-naphtoic acid, according to its UV, mass and NMR spectra, and a minor
compound with HPLC R
t
and UV spectrum indistinguishable from 5-methylsalicylate. The identification of those metabolites, and the demonstration
of 2,3-catechol dioxygenase activity in 2-MP induced cells show that the biodegradation of 2-MP by strain JS5 is initiated
via dioxygenation and meta-cleavage of the non-methylated aromatic ring, and then proceeds by reactions similar to those reported for phenanthrene.
Incubation of the strain with a MP-containing mixture from a pyrolytic fuel oil demonstrates that strain JS5 also acts on
other methylated phenanthrenes.
Received: 28 December 1998 / Received revision: 21 June 1999 / Accepted: 27 June 1999 相似文献
13.
When grown on vegetable oils and their derivatives, the smut fungus Ustilago maydis (DSM 4500 and ATCC 14826) produces several glycolipids under nitrogen-limiting conditions. With 45 g l−1 sunflower oil fatty acids (technical grade) a yield of 30 g l−1 glycolipid was achieved. The resulting mixture contained predominantly mannosylerythritol lipids together with smaller amounts
of cellobiose lipids. The production of the more polar cellobiose lipids was enhanced when glucose was used as carbon source.
The molecular structure of the main components of the glycolipid mixture were elucidated by a combination of NMR spectroscopic
and mass-spectrometric techniques.
Received: 22 June 1998 / Received revision: 11 September 1998 / Accepted: 13 September 1998 相似文献
14.
M. Wenk T. Baumgartner J. Dobovšek T. Fuchs J. Kucsera J. Zopfi G. Stucki 《Applied microbiology and biotechnology》1998,49(5):624-630
The evaluation of pesticide-mineralising microorganisms to clean-up contaminated soils was studied with the widely applied
and easily detectable compound atrazine, which is rapidly mineralised by several microorganisms including the Pseudomonas sp. strain Yaya 6. The rate of atrazine removal was proportional to the water content of the soil and the amount of bacteria
added to the soil. In soil slurry, 6 mg atrazine kg soil−1 was eliminated within 1 day after application of 0.3 g dry weight inoculant biomass kg soil−1 and within 5 days when 0.003 g kg soil−1 was used. In partially saturated soil (60% of the maximal water-holding capacity) 15 mg atrazine kg soil−1 was eliminated within 2 days by 1 g biomass kg soil−1 and within 25 days when 0.01 g biomass kg soil−1 was used. In unsaturated soil, about 60% [U-ring-14C]atrazine was converted to 14CO2 within 14 days. Atrazine was very efficiently removed by the inoculant biomass, not only in soil that was freshly contaminated
but also in soil aged with atrazine for up to 260 days. The bacteria exposed to atrazine in unsaturated sterile soil were
still active after a starvation period of 240 days: 15 mg newly added atrazine kg soil−1 was eliminated within 5 days.
Received: 31 October 1997 / Received revision: 16 January 1998 / Accepted: 18 January 1998 相似文献
15.
M. Rutgers A. M. Breure J. G. van Andel W. A. Duetz 《Applied microbiology and biotechnology》1997,48(5):656-661
A polychlorophenol-degrading bacterium, Sphingomonas sp. strain P5, was grown in 2,6-dichlo-rophenol(26-DCP)-limited, 2,3,6-trichlorophenol(236-TCP)-limited, 2,4,6-trichlorophenol(246-TCP)-limited,
2,3,4,6-tetrachlorophenol(2346-TeCP)-limited, and pentachlorophenol(PCP)-limited chemostat cultures at a dilution rate of
0.02 ± 0.002 h−1. The cultures were analyzed for the yield coefficient for growth on chlorophenol during steady-state conditions. The average
growth yields coefficients (as carbon conversion efficiencies) were 0.252, 0.230, 0.219, 0.157, and 0.121 mol C mol C−1 for 26-DCP, 236-TCP, 246-TCP, 2346-TeCP, and PCP respectively. The differences in growth yield can be interpreted in terms
of the energetics of chlorinated carbon metabolism; i.e. substitution of the phenol moiety reduces the available metabolic
energy by one electron per chlorine. The growth yield coefficients on chlorinated phenols were lower than the yield coefficients
of heterotrophic growth reported in the literature on non-chlorinated and aliphatic compounds. Metabolic origins for low growth
yield coefficients on (chlorinated) aromatic compounds are postulated.
Received: 7 April 1997 / Received revision: 7 July 1997 / Accepted: 12 July 1997 相似文献
16.
R S English J S Lampel TJ Vanden Boom 《Journal of industrial microbiology & biotechnology》1998,21(4-5):219-224
The introduction of plasmid DNA into germinating spores of an industrially improved strain of Saccharopolyspora erythraea was accomplished by electroporation. Various parameters affecting the efficiency of electroporation were examined. The most
critical factor was the extent of spore germination. Electrocompetence was limited to a 4-h period following the initial emergence
of the germ tube. Electroporation efficiencies as high as 2 × 105 CFU μg−1 of plasmid DNA were obtained using electrocompetent germlings. The optimal field strength was 12–14 kV cm−1 with a pulse duration of 15–20 ms. Electrocompetent germlings were stored at −80°C without a significant decrease in transformation
efficiency. The utility of this protocol was demonstrated by isolating a propionyl-CoA carboxylase mutant through targeted
gene disruption and replacement.
Received 3 April 1998/ Accepted in revised form 28 September 1998 相似文献
17.
Polymer production by two newly isolated extremely halophilic archaea: application of a novel corrosion-resistant bioreactor 总被引:3,自引:0,他引:3
Hezayen FF Rehm BH Eberhardt R Steinbüchel A 《Applied microbiology and biotechnology》2000,54(3):319-325
A novel corrosion-resistant bioreactor composed of polyetherether ketone (PEEK), tech glass and silicium nitrite ceramics
was constructed and applied for the cultivation of two newly isolated, extremely halophilic archaea producing poly(γ-glutamic
acid) (PGA), or poly(β-hydroxy butyric acid) (PHB), respectively. These bacteria were isolated from hypersaline soil close
to Aswan (Egypt). The isolate strain 40, which is related to the genus Natrialba, produced large amounts of PGA when cultivated on solid medium. Culture conditions were optimised applying the corrosion-resistant
bioreactor. PGA production was dependent on NaCl concentration and occurred about at 20% (w/v) NaCl in the medium. A maximum
cell density of about 1.6 g cell dry matter/l was obtained when the bioreactor was stirred and aerated in a batch fermentation
process using proteose-peptone medium. The supernatant was monitored with respect to PGA formation, and after 90 h a maximum
of 470 mg/l culture volume was detected by HPLC analysis. Culture conditions were optimized for the isolate 56, which accumulated
PHB as intracellular granules. Batch fermentations in the stirred and aerated bioreactor applying acetate and n-butyric acid as carbon sources led to cell density of 2.28 g cell dry matter/l and a maximum PHB accumulation contributing
to about 53% of cellular dry weight. About 4.6 g PHB were isolated from 10.6 g dried cells of strain 56, which exhibited a
weight average molar mass of 2.3 × 105 g mol−1 and a polydispersity of about 1.4.
Received: 3 December 1999 / Received revision: 22 February 2000 / Accepted: 25 February 2000 相似文献
18.
Initiation of [36Cl]hexachlorobenzene dechlorination in three different soils under artificially induced anaerobic conditions 总被引:1,自引:0,他引:1
P. Rosenbrock R. Martens F. Buscot J. C. Munch 《Applied microbiology and biotechnology》1997,48(1):115-120
The potential for reductive dechlorination of hexachlorobenzene was investigated in samples of three different, naturally
oxic soils held under conditions of high oxygen deficiency. The soils were water-saturated and the influence on dechlorination
of adding different electron donors, a surfactant and an anaerobic microbial consortium was tested. The influence of supplied
electron donors seems to depend on the organic matter content of the soils. Dechlorination in the organic-matter-rich soil
from Maulach was not affected by amendment with organic electron donors. A release of about 40% chloride within 140 days was
observed for this soil in all biotic-treated assays. By contrast, the organic-matter-poor soil of Eppingen showed no dechlorination
in unamended assays. However, when it was supplemented with organic electron donors dechlorination of 2%–37% occurred within
140 days, depending on the type of electron donor. Complex substrate (wheat strawdust), from which carbon is slowly liberated,
gave the best results. These two soils had an indigenous dechlorinating anaerobic microflora, whereas the third soil (Rastatt)
required inoculation with an anaerobic consortium for dechlorination. The addition of electron donors alone did not cause
dechlorination in this sandy soil. The addition of a surfactant (Tween 80) to increase the bioavailability of hexachlorobenzene
did not enhance dechlorination. This process was not inhibited by inherent alternative electron acceptors in soil (NO3−, SO4
2−, Fe3+). The dechlorination did not require methanogenic conditions.
Received: 12 December 1996 / Received revision: 14 March 1997 / Accepted: 15 March 1997 相似文献
19.
The microbial degradation of hydrocarbons was studied in an artificially contaminated sandy soil, using a pilot-scale percolator
system. After a short lag period, an intensive degradation occurred, which diminished in time and completely stopped in the
end, despite large residual contaminations (residues of 56% diesel fuel, 20% n-hexadecane and 3.5% phenanthrene at the initial loadings of each 3000 mg/kg). The remaining pollutant content was influenced
by the kind of hydrocarbon but was nearly independent of its initial loading. According to a model-aided analysis of the carbon
dioxide production during remediation, the observed stagnation of degradation was caused by a limited bioavailability of the
pollutants. The degradation in the soil-free aqueous phase was more extensive than in the soil, which suggests that the limited
bioavailability in the soil can be attributed mainly to matrix-dependent rather than substrate-dependent influences. Generally,
fine particles and organic matter are mainly responsible for the adsorption of pollutants to the soil matrix. Our sandy soil
also bound hydrocarbons adsorptively although it contained neither silty material nor significant amounts of organic matter.
As shown by Brunauer Emmett Teller (BET) analysis, the soil particles were covered by micropores, which enlarged the soil
surface by a factor of 120 in comparison with the macroscopic surface area. The microporosity is the reason for the hydrocarbons
being more strongly adsorbed to the sandy soil than expected.
Received: 13 July 1998 / Received revision: 22 September 1998 / Accepted: 26 September 1998 相似文献
20.
Cometabolic biodegradation of methyl t-butyl ether by Pseudomonas aeruginosa grown on pentane 总被引:1,自引:0,他引:1
A bacterial strain identified as Pseudomonas aeruginosa was isolated from a soil consortium able to mineralize pentane. P. aeruginosa could metabolize methyl t-butyl ether (MTBE) in the presence of pentane as the sole carbon and energy source. The carbon balance for this strain, grown
on pentane, was established in order to determine the fate of pentane and the growth yield (0.9 g biomass/g pentane). An inhibition
model for P. aeruginosa grown on pentane was proposed. Pentane had an inhibitory effect on growth of P. aeruginosa, even at a concentration as low as 85 μg/l. This resulted in the calculation of the following kinetic parameters (μmax = 0.19 h−1, K
s = 2.9 μg/l, K
i = 3.5 mg/l). Finally a simple model of MTBE degradation was derived in order to predict the quantity of MTBE able to be degraded
in batch culture in the presence of pentane. This model depends only on two parameters: the concentrations of pentane and
MTBE.
Received: 16 July 1998 / Received revision: 11 November 1998 / Accepted 31 November 1998 相似文献