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1.
In Manduca sexta, the blastoderm forms successively and becomes immediately cellularized as the cleavage energids reach the surface of the oocyte. Presumptive serosal cells are large and contain 2 or 4 large polyploid nuclei; presumptive embryonic cells are small and mononuclear. All parts of the blastoderm participate in the uptake and digestion of yolk material. About 10 h post-oviposition, the blastoderm breaks at the amnioserosal fold and the extraembryonic part closes above the germ band and constitutes the serosa (12 h post-oviposition, i.e. 10% development completed). At once, the serosa starts to secrete a cuticle consisting of an epi- and a lamellated endocuticle. Detachment of the serosal cuticle, 22h post-oviposition, is reminiscent of apolysis of larval cuticle. Thereafter, the serosa deposits a membranous structure, the serosal membrane. The sercretory process lasts from 23h to 44h post-oviposition. At first a fine granular layer, then an amorphous, spongy-like, fibrillar layer is secreted via microvilli. This persisting membrane is tough, rubbery and very elastic. It may serve to bolster the serosa during katatrepsis (48h post-oviposition) and later embryonic movements. After detachment of the serosal membrane, 44h post-oviposition, a distinct subcellular reorganization of the serosa takes place. The nuclei become still larger and more irregular. Uptake of yolk granules, but not of lipid droplets, ceases, although interaction of serosa and yolk cells are intense. Serosal cells include many mitochondria, large areas of rER, besides some sER, increasing amounts of lysosomal bodies and prominent Golgi complexes. Most conspicuous is the assembly of spindle-shaped, electron-lucent vesicles below the apical surface. These vesicles may contain metabolic products which are released into the peripheral space. The studies show that the serosa assumes changing functions during embryogenesis: digestion of yolk substances, synthesis of a serosal cuticle and a serosal membrane, which may have a protective function, and excretion. 相似文献
2.
Deposition, detachment and removal of the three embryonic cuticles are studied. The menbrane-like cuticle 1 covers the embryo during katatrepsis and ‘disappears’ thereafter. Cuticle 2 deposition starts shortly before dorsal closure. Its apolysis is accompanied by contractions of the embryo. Ecdysis of cuticle 2 takes place during hatching. Only cuticle 3 (= first larval cuticle) shows differentiations like sensilla and cornea. Peaks of ecdysteroid (and probably JH) titre are observed during apolysis of cuticle 1 and cuticle 2 (Dorn, 1981). Transition from ectoderm to epidermis proper takes place shortly before and during onset of cuticle 2 synthesis. 相似文献
3.
《International Journal of Insect Morphology and Embryology》1981,10(3):203-224
The appearance and development of the dorsal organ of collembolan embryos were analyzed by electron microscopy, to get a better insight into the cytodifferentiation and properties of the cells that constitute the organ.When the blastular epithelium surrounds the yolk as a thick cellular covering, the veryfirst rudiment of the dorsal organ can be detected as a somewhat thicker calotte of cells, particularly rich in lipid globules. Rapid morphogenetic processes result in the concentration of this first rudiment into a lens-shaped primordium, whose median cells sink into the yolk at their basal poles. All the cells of the dorsal organ contribute by their apical surfaces to the secretion of the first blastodermic membrane, a very atypical equivalent of an external epicuticle, then to the deposition of the outer layers of the second blastodermic cuticle.The dorsal organ takes the form of a plug and its central cells become columnar. Their considerable growth is marked by the internal presence of numerous regularly grouped microtubules, that will later contribute to the mechanical support of their apical processes. The blastoderm and the dorsal organ then secrete the procuticular portion of the second blastodermic cuticle (folded membrane of other authors). This inner part shows a very regular alternation of dense and less dense layers, eventual clues for a rapid, non-circadian, rhythm of activity in the cells.When the embryonic band becomes molded, the central cells of the dorsal organ send out very long apical processes, whose terminal contacts with the second cuticle, now swollen apart from the surface of the egg, in our opinion might play a role in the control of cuticle permeability. When the embryo grows older, the dorsal organ gets into its presumed functional phase; meanwhile, its constituent cells become diversified into several lines, according to a concentric pattern. The central cells are then surrounded by two cellular rings whose components show different amplifications, leaflets and microvilli, of their apical membranes. These cells are eventually supposed to play a role in exchanges with the external fluid.The involution of the dorsal organ begins at a relatively young stage of the embryonic band and continues during embryonic organogenesis. 相似文献
4.
《Arthropod Structure & Development》2014,43(5):423-439
Exoskeletal crustacean cuticle is a calcified apical extracellular matrix of epidermal cells, illustrating the chitin-based organic scaffold for biomineralization. Studies of cuticle formation during molting reveal significant dynamics and complexity of the assembly processes, while cuticle formation during embryogenesis is poorly investigated. This study reveals in the terrestrial isopod Porcellio scaber, the ultrastructural organization of the differentiating precuticular matrices and exoskeletal cuticles during embryonic and larval intramarsupial development. The composition of the epidermal matrices was obtained by WGA lectin labelling and EDXS analysis. At least two precuticular matrices, consisting of loosely arranged material with overlying electron dense lamina, are secreted by the epidermis in the mid-stage embryo. The prehatching embryo is the earliest developmental stage with a cuticular matrix consisting of an epicuticle and a procuticle, displaying WGA binding and forming cuticular scales. In newly hatched marsupial larva manca, a new cuticle is formed and calcium sequestration in the cuticle is evident. Progression of larval development leads to the cuticle thickening, structural differentiation of cuticular layers and prominent cuticle calcification. Morphological characteristics of exoskeleton renewal in marsupial manca are described. Elaborated cuticle in marsupial larvae indicates the importance of the exoskeleton in protection and support of the larval body in the marsupium and during the release of larvae in the external environment. 相似文献
5.
M. Lagueux C. Hetru F. Goltzene C. Kappler J.A. Hoffmann 《Journal of insect physiology》1979,25(9):709-723
Eggs of Locusta migratoria contain remarkably high concentrations of ecdysone and several other ecdysteroids. During the time-span of embryonic development (11 days) 4 distinct peaks of ecdysone concentration (up to 8 μM) are observed in the egg, demonstrating the ecdysiosynthetic capacity of the embryo. Only during postblastokinetic development, is ecdysone efficiently hydroxylated to 20-hydroxyachieved through conjugation. On the basis of optical and electron microscopic observations, we have been able to correlate precisely each of the four peaks of ecdysone concentration in the egg with the time of deposition of a cuticle by the embryonic tissues (peak 1: serosal cuticle; peak 2: first embryonic cuticle; peak 3: second embryonic cuticle; peak 4: third embryonic cuticle). 相似文献
6.
7.
Structure and expression of a Manduca sexta larval cuticle gene homologous to Drosophila cuticle genes 总被引:5,自引:0,他引:5
A genomic clone was isolated from the tobacco hornworm, Manduca sexta, by virtue of its similarity to a Drosophila larval cuticle gene. RNA analysis shows that this clone, B311, is expressed at times appropriate for a larval cuticle gene. Hybrid-selection experiments using B311 DNA show that it encodes a 14 x 10(3) Mr protein, LCP-14, which is precipitated by an antiserum to Manduca larval cuticle. We have sequenced both genomic and cDNA clones for the LCP-14 gene. A conceptual translation of the cDNA sequence shows that the LCP-14 protein is similar not only to another Manduca cuticle protein, but also to Drosophila, Sarcophaga and Hyalophora cecropia cuticle proteins. Since these proteins are found in flexible cuticle and have similar sequences, we conclude they are encoded by homologous genes. 相似文献
8.
Isolation and embryonic expression of an abdominal-A-like gene from the lepidopteran, Manduca sexta. 总被引:3,自引:0,他引:3
Using sequence homology to the Drosophila Antennapedia gene, we isolated a homeobox-containing gene from the lepidopteran, Manduca sexta. Sequence analysis and in situ hybridizations to tissue sections suggest that the Manduca gene encodes a lepidopteran homologue of the Drosophila Bithorax complex gene abdominal-A. The predicted amino acid sequence of a 76 amino acid region that includes the homeobox and the regions immediately flanking it are identical between the Manduca and Drosophila genes. Northern blots reveal that the manduca abd-A gene is expressed first in the early embryo and continues to be expressed throughout later embryonic and larval stages. In situ hybridizations show that the posterior half of the first abdominal segment marks the anterior border of the Manduca abd-A expression. This expression pattern demonstrates the conservation of parasegments as domains of gene activity in the lepidopteran embryo. The Manduca abd-A expression extends from the posterior half of the first abdominal segment through the tenth abdominal segment, a domain that is greater than that of the Drosophila abd-A expression, and reflects the difference in visible segment number between the two insects. 相似文献
9.
Embryonic and post-embryonic development of the Early Cambrian cnidarian Olivooides 总被引:12,自引:0,他引:12
YUE ZHAO STEFAN BENGTSON 《Lethaia: An International Journal of Palaeontology and Stratigraphy》1999,32(2):181-195
Phosphatized specimens of Olivooides from the Early Cambrian of Shaanxi, China, represent a number of developmental stages. These include cleavage, gastrulation, organogenesis, cuticularization, pre-hatching, post-hatching and subsequent growth. This allows the reconstruction of a nearly full developmental sequence of this animal. Olivooides had large (600-870 μm in diameter), sphaerical eggs, indicating a high yolk content. Development was direct. Thus adult characters were forming already in the embryo, and there was no free larval stage. The embryonic development took place within a smooth protective membrane. Gastrulation probably was by polar ingression, and the blastopore appears to correspond to the aperture of the later stages. An embryonic cuticle formed which carried star-shaped structures, stellae, over the entire surface except for a radially folded non-stellate portion around the future aperture. At a later stage, the stellate cuticle was thrown into folds concentric with the aperture. This radially folded tissue then became more dominant. After hatching, the body assumed the shape of a strongly annulated cone, with the stellate cuticle forming the apical part and the folded cuticle forming a longitudinally striate cuticle around the aperture. Subsequent growth took place through the addition of striate tissue. A pentaradial symmetry of the body is suggested by lateral folds in the apical part. Olivooides is interpreted as a cnidarian, probably closely related to the scyphozoans. The conical test may have housed a polyp similar to the thecate polyps of modern coronate scyphozoans, but, unlike the latter, Olivooides had no visible attachment structures. There is no evidence for or against a free medusa stage. The prevalence of lecithotrophic direct developers in the Neoproterozoic and Cambrian, unless reflecting a preservational bias, casts some doubts on evolutionary models that assume larval planktotrophy to be primitive among metazoans. 相似文献
10.
The granular phenoloxidase (PO) that is responsible for cuticular melanization in Manduca sexta larva was purified and an antibody was prepared. This granular PO was found to consist of four isozymes of 90 kDa with isoelectric points ranging from 5.7 to 5.85. The enzyme was immunologically and electrophoretically distinct from the cuticular wound PO, a second cuticular PO common to all larval cuticle, and the hemolymph PO. Both [14C]mannose and [14C]sialic acid were incorporated into the granular PO, showing that this granular PO was a glycoprotein whose sugar moiety was a complex oligosaccharide. When no juvenile hormone (JH) was present at the head capsule slippage (HCS) stage, the epidermis began synthesizing PO 6 hr later. This epidermal synthesis was maximal 12 hr after HCS at which time the PO appeared in the cuticle, and then synthesis declined. When synthesis ceased about 23 hr after HCS, no further incorporation into the cuticle was observed. As melanization proceeded, immunologically detectable cuticular PO decreased. Application of 0.1 microgram JH I at the time of HCS inhibited synthesis of PO by the epidermis and thus prevented melanization. JH application after PO synthesis had begun (8 hr after HCS) prevented its subsequent synthesis, causing partial melanization. Thus, the absence of JH is necessary during the period of epidermal synthesis of the granular PO to allow complete melanization. 相似文献
11.
The sequential synthesis and deposition of larval cuticular proteins was followed during the final larval molt and the final larval instar of the tobacco hornworm Manduca sexta and correlated with changes in cuticular structure. On the final day of feeding (Day 3) before the onset of metamorphosis many endocuticular proteins were no longer synthesized and new isoelectric variants of 27,000-Da polypeptides were deposited into the cuticle coincident with the formation of lamellae 5- to 10-fold thinner than those previously deposited. Application of a juvenile hormone analog methoprene on Day 1 prevented this change in protein synthesis and in lamellar structure by preventing the observed rise in the intermolt ecdysteroid titer on Day 2. These changes could be induced in vitro by 25-100 ng/ml 20-hydroxyecdysone in the absence of juvenile hormone. Thus, the intermolt change in the lamellar assembly process appears to result from hormone-induced changes in cuticular protein synthesis. 相似文献
12.
Epidermal cell morphology and cuticle production in Manduca sexta are directly influenced by both ecdysterone and juvenile hormone. Up to day 6 of the last larval instar, post-molt endocuticle is continuously deposited even though cells undergo a partial and temporary separation from the overlying cuticle at the time when a small ecdysteroid peak is detected (approximately day 3.5). At about days 6--7 when another, larger ecdysteroid peak is present, apolysis occurs accompanied by the appearance of edcysial droplets. Following apolysis, layers of pupal cuticle are deposited. Increased quantities of rough endoplasmic reticulum characterize the epidermis at times of peak endocuticle deposition (day 3, larval cuticle; day 9, pupal cuticle). Dense pigment inclusions are found in epidermis from the day of ecdysis to the last larval instar until they are eliminated 5 days later. These dense bodies migrate from cell apex to base in the absence of juvenile hormone (or in the presence of a negligible amount of juvenile hormone) and probably contain insecticyanin. 相似文献
13.
Gina Temin Malcolm Zander Jean-Pierre Roussel 《Invertebrate reproduction & development.》2013,57(1):105-112
Summary The titre fluctuations of juvenile hormone III (JH III) in eggs of Locusta during embryogenesis were investigated by use of a combination of gas chromatography and chemical ionisation mass spectrometry. The results do not favour the idea of a functional transfer of JH III from the adult female to the embryo. JH III was found to be absent during the period of intense, JH-sensitive, organogenesis, roughly at mid-embryogenesis. The hormone titres peak shortly before the deposition of the last (3rd) embryonic cuticle (which corresponds to the first larval cuticle). Hatching occurs in the virtual absence of JH III. 相似文献
14.
The hindgut and foregut in terrestrial isopod crustaceans are ectodermal parts of the digestive system and are lined by cuticle, an apical extracellular matrix secreted by epithelial cells. Morphogenesis of the digestive system was reported in previous studies, but differentiation of the gut cuticle was not followed in detail. This study is focused on ultrastructural analyses of hindgut apical matrices and cuticle in selected intramarsupial developmental stages of the terrestrial isopod Porcellio
scaber in comparison to adult animals to obtain data on the hindgut cuticular lining differentiation. Our results show that in late embryos of stages 16 and 18 the apical matrix in the hindgut consists of loose material overlaid by a thin intensely ruffled electron dense lamina facing the lumen. The ultrastructural resemblance to the embryonic epidermal matrices described in several arthropods suggests a common principle in chitinous matrix differentiation. The hindgut matrix in the prehatching embryo of stage 19 shows characteristics of the hindgut cuticle, specifically alignment to the apical epithelial surface and a prominent electron dense layer of epicuticle. In the preceding embryonic stage – stage 18 – an electron dense lamina, closely apposed to the apical cell membrane, is evident and is considered as the first epicuticle formation. In marsupial mancae the advanced features of the hindgut cuticle and epithelium are evident: a more prominent epicuticular layer, formation of cuticular spines and an extensive apical labyrinth. In comparison to the hindgut cuticle of adults, the hindgut cuticle of marsupial manca and in particular the electron dense epicuticular layer are much thinner and the difference between cuticle architecture in the anterior chamber and in the papillate region is not yet distinguishable. Differences from the hindgut cuticle in adults imply not fully developed structure and function of the hindgut cuticle in marsupial manca, possibly related also to different environments, as mancae develop in marsupial fluid. Bacteria, evenly distributed within the homogenous electron dense material in the hindgut lumen, were observed only in one specimen of early marsupial manca. The morphological features of gut cuticle renewal are evident in the late marsupial mancae, and are similar to those observed in the exoskeleton. 相似文献
15.
The embryonic development of the hemipteran insect Rhodnius prolixus was studied by use of contemporary light and electron microscopy. Embryos were staged according to days postoviposition. Eggs laid on day one complete blastoderm formation and anatrepsis, the first phase of blastokinesis, by day 5. The embryo develops in a cephalocaudal orientation which is 180° to the anteroposterior axis of the egg. Subsequent development, prior to the second phase of blastokinesis (katatrepsis), leads to segmentation of the germ band, evagination of appendages, and histogenesis of germ layers. Concomitantly with these events, the amnion undergoes dramatic change. By day 7 the embryo begins a 180° revolution while migrating to the ventral surface of the yolk. This restores its polarity with respect to that of the egg and facilitates hatching. The serosa contracts, pulling the amnion and embryo anteriorly. Eventually the serosa is internalized at a point dorsal to the head and the lateral walls of the embryo grow up and surround the yolk. Development continues until day 15 when the embryo hatches as a first instar larva. 相似文献
16.
Juvenile hormone acts at embryonic molts and induces the nymphal cuticle in the direct-developing cricket 总被引:1,自引:0,他引:1
During embryogenesis of hemimetabolous insects, the sesquiterpenoid hormone, juvenile hormone (JH), appears late in embryogenesis coincident with formation of the first nymphal cuticle. We tested the role of embryonic JH by treating cricket embryos with JH III, or the JH-mimic (JHM) pyriproxifen, during early embryogenesis. We found two discrete windows of JH sensitivity. The first occurs during the formation of the first (E1) embryonic cuticle. Treatment with JHM prior to this molt produced small embryos that failed to complete the movements of katatrepsis. Embryos treated after the E1 molt but before the second embryonic (pronymphal) molt completed katatrepsis but then failed to complete dorsal closure and precociously formed nymphal, rather than pronymphal characters. This second sensitivity window was further assessed by treating embryos with low doses of JH III prior to the pronymphal molt. With low doses, mosaic cuticles were formed, bearing features of both the pronymphal and nymphal stages. The nymphal characters varied in their sensitivity to JH III, due at least in part to differences in the timing of their sensitivity windows. Unexpectedly, many of the JH III-treated embryos with mosaic and precocious nymphal cuticles made a second nymphal cuticle and successfully hatched. JH treatment also affected the growth of the embryos. By focusing on the developing limb, we found that the effect of JH upon growth was asymmetric, with distal segments more affected than proximal ones, but this was not reflected in misexpression of Distal-less or Bric-a-brac, which are involved in proximal-distal patterning of the limb.Edited by P. Simpson 相似文献
17.
Summary Before the shortening of the germ band, embryos of Calliphora erythrocephala secrete an electron dense layer which resembles a thin atypical cuticle. After completion of the dorsal closure, a second, typical, cuticle is deposited. It is characterized by a thick procuticle and by the presence of hooked setae. This cuticle develops into the larval cuticle of the first instar. We did not find in newly-laid eggs of Calliphora detectable amounts of either free or hydrolysable conjugated ecdysteroids. A marked rise in ecdysteroid concentrations, essentially attributable to free ecdysone and 20- hydroxyecdysone, occurs shortly before the deposition of the typical cuticle. After a transient decrease, the ecdysteroid titre rises again before hatching. When eggs are mid-ligatured at blastula or early gastrula stages, the posterior embryonic half is able to build up a typical cuticle with hooked setae. This cuticulogenesis is therefore not dependent on the presence of the embryonic ring glands. 相似文献
18.
Unlike most Hox cluster genes, with their canonical role in anterior-posterior patterning of the embryo, the Hox3 orthologue of insects has diverged. Here, we investigate the zen orthologue in Oncopeltus fasciatus (Hemiptera:Heteroptera). As in other insects, the Of-zen gene is expressed extraembryonically, and RNA interference (RNAi) experiments demonstrate that it is functionally required in this domain for the proper occurrence of katatrepsis, the phase of embryonic movements by which the embryo emerges from the yolk and adjusts its orientation within the egg. After RNAi knockdown of Of-zen, katatrepsis does not occur, causing embryos to complete development inside out. However, not all aspects of expression and function are conserved compared to grasshopper, beetle, and fly orthologues. Of-zen is not expressed in the extraembryonic tissue until relatively late, suggesting it is not involved in tissue specification. Within the extraembryonic domain, Of-zen is expressed in the outer serosal membrane, but unlike orthologues, it is not detectable in the inner extraembryonic membrane, the amnion. Thus, the role of zen in the interaction of serosa, amnion, and embryo may differ between species. Of-zen is also expressed in the blastoderm, although this early expression shows no apparent correlation with defects seen by RNAi knockdown. 相似文献
19.
The patch-clamp technique was applied to the apical membrane of epithelial midgut cells of a lepidoptera, Manduca sexta L. Access to the apical membrane, the main target site of Bacillus thuringiensis (Bt) toxins, was achieved by using freshly isolated larval midgut preparations mounted onto holding glass pipettes. The epithelial cells retained their functional integrity, as evidenced by the magnitude of intracellular potentials recorded with microelectrodes. With standard 32 mM K(+) solution in the bath and the patch-clamp pipette, endogenous channel activity was detected in about 50% of experiments, mainly in moulting larvae and larvae that had been kept at reduced temperature for at least two days prior to the experiments. In both cell-attached and inside-out patch-clamp configurations, different types of channel were observed, with conductances varying between about 5 and 50 pS and different conducting properties. Addition of trypsin-activated Cry1Ac Bt toxin in the patch-clamp pipette triggered, after a delay, large conductances of a few nanosiemens. This is the first study allowing exploration, in the intact midgut, of the properties of apical membrane channels and the direct interaction between the apical membrane of epithelial cells and pathogenic agents such as Bt toxins. 相似文献
20.
A variety of semi-defined artificial diets were developed and tested for their ability to support the in vitro development of Edovum puttleri. In the most effective diet, 2.6% of E. puttleri pupated. This diet contained high levels of hen egg yolk combined with Manduca sexta larval hemolymph, or with a mixture of M. sexta egg homogenate and larval hemolymph. Egg homogenate alone (without the addition of hemolymph) was not capable of supporting the parasitoid's development. Thus, hemolymph appears to contain unidentified factor(s) important for inducing pupation of the wasp. Addition of M. sexta pupal fat body tissue extract (in place of hemolymph) also promoted pupation of E. puttleri. Gypsy moth (Lymantria dispar) larval hemolymph could not replace M. sexta larval hemolymph. Fractionation irreversibly reduced the growth-promoting effects of M. sexta larval hemolymph. However, the most effective fraction contained components whose molecular weights were 1000 kd. In diets that were devoid of insect materials, the best results were achieved when hen egg yolk, FreAmine, yeast extract, lactalbumin, trehalose, fetal bovine serum and bovine milk were included. This is the first report of an artificial diet for in vitro rearing an eulophid parasitoid from the egg through the pupal stage. 相似文献