共查询到20条相似文献,搜索用时 15 毫秒
1.
Fulvia Tambone Pierluigi Genevini Giuliana D’ImporzanoFabrizio Adani 《Bioresource technology》2009,100(12):3140-3142
The transformation of organic matter during anaerobic digestion of mixtures of energetic crops, cow slurry, agro-industrial waste and organic fraction of municipal solid waste (OFMSW) was studied by analysing different samples at diverse points during the anaerobic digestion process in a full-scale plant. Both chemical (fiber analysis) and spectroscopic approaches (13C CPMAS NMR) indicated the anaerobic digestion process proceeded by degradation of more labile fraction (e.g. carbohydrate-like molecules) and concentration of more recalcitrant molecules (lignin and non-hydrolysable lipids). These modifications determined a higher degree of biological stability of digestate with respect to the starting mixture, as suggested, also, by the good correlations found between the cumulative oxygen uptake (OD20), and the sum of (cellulose + hemicellulose + cell soluble) contents of biomasses detected by fiber analysis (r = 0.99; P < 0.05), and both O–alkyl-C (r = 0.98; P < 0.05) and alkyl-C (r = −0.99; P < 0.05) measured by 13C CPMAS NMR. 相似文献
2.
Baudrand R Campino C Carvajal CA Olivieri O Guidi G Faccini G Sateler J Cornejo J Martin BS Dominguez JM Cerda J Mosso LM Owen GI Kalergis AM Fardella CE 《Steroids》2011,76(14):1575-1581
Metabolic syndrome (MetS) may have increased cortisol (F) production caused by 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) in liver and adipose tissue and/or by HPA axis dysregulation. F is then mainly metabolized by liver reductases into inactive tetrahydrometabolites (THMs). We measured THM levels in patients with or without MetS and evaluate the correlation between THMs and anthropometric and biochemical parameters. We recruited 221 subjects, of whom 130 had MetS by ATP III. We evaluated F, cortisone (E), adipokines, glucose, insulin and lipid profiles as well as urinary (24 h) F, E and THM levels. β Cell function was estimated by the HOMA Calculator. We observed that patients with MetS showed higher levels of THMs, HOMA-IR and leptin and lower levels of adiponectin and HOMA-β but no differences in F and E in plasma or urine. THM was associated with weight (r = +0.44, p < 0.001), waist circumference (r = +0.38, p < 0.01), glycemia (r = +0.37, p < 0.01), and triglycerides (r = +0.18, p = 0.06) and negatively correlated with adiponectin (r = −0.36, p < 0.001), HOMA-β (r = −0.21, p < 0.001) and HDL (r = −0.29, p < 0.01). In a logistic regression model, THM levels were associated with hypertension, hyperglycemia and dyslipidemia. We conclude that MetS is associated with increased urinary THMs but not with F and E levels in plasma or urine. Increased levels of THM, reflecting the daily cortisol production subsequently metabolized, are correlated with hypoadiponectinemia, hypertension, dyslipidemia, insulin resistance and β cell dysfunction. A subtle increased in glucocorticoid production may further account for the phenotypic and biochemical similarities observed in central obesity and Cushing’s syndrome. 相似文献
3.
Berenguer J Fernandez-Rodríguez A Jimenez-Sousa MA Cosín J Zarate P Micheloud D López JC Miralles P Catalán P Resino S 《Cytokine》2012,57(1):25-29
Background
CXCL10 may contribute to the host immune response against the hepatitis C virus (HCV), liver disease progression, and response to HCV antiviral therapy. The aim of our study was to analyze the relationship among virological, immunological, and clinical characteristics with plasma CXCL10 levels in human immunodeficiency virus (HIV)/HCV-coinfected patients.Methods
We carried out a cross-sectional study on 144 patients. CXCL10 and insulin were measured using an immunoassay kit. The degree of insulin resistance was estimated for each patient using the homeostatic model assessment (HOMA) method. Insulin resistance was defined as a HOMA index higher than or equal to 3.8. Aspartate aminotransferase (AST) to platelet ratio (APRI), FIB-4, Forns index, HGM1, and HGM2 were calculated.Results
The variables associated with log10 CXCL10 levels by univariate analysis were age (b = 0.013; p = 0.023), prior AIDS-defining condition (b = 0.127; p = 0.045), detectable plasma HIV viral load (b = 0.092; p = 0.006), log10 HOMA (b = 0.216; p = 0.002), HCV-genotype 1 (b = 0.114; p = 0.071), and liver fibrosis assessed by all non-invasive indexes (log10 APRI (b = 0.296; p = 0.001), log10 FIB-4 (b = 0.436; p < 0.001), log10 Forns index (b = 0.591; p < 0.001), log10 HGM1 (b = 0.351; p = 0.021), and log10 HGM2 (b = 0.215; p = 0.018)). However, in multivariate analysis, CXCL10 levels were only associated with HOMA, detectable plasma HIV viral load, HCV-genotype 1 and FIB-4 (R-square = 0.235; p < 0.001).Conclusion
Plasma CXCL10 levels were influenced by several characteristics of patients related to HIV and HCV infections, insulin resistance, and liver fibrosis, indicating that CXCL10 may play an important role in the pathogenesis of both HCV and HIV infections. 相似文献4.
Studies on 2d:4d, the ratio between the second and the fourth digit, as a possible indicator of prenatal androgen exposure, have failed to produce consistent results. This paper analyzes the relation between 2d:4d, sex steroids and well-documented sex differences in characteristics such as depression, dominance, and aggressive (ART) and non-aggressive adolescent risk-taking (NART) in a comparatively large sample of adolescent boys (N = 301, mean age: 14.4 years) and girls (N = 298, mean age: 14.3 years). Boys had on average a lower 2d:4d than girls (F = 42.15; p < 0.001). With respect to boys, controlling for age and pubertal development (PD), a small but marginally significant positive association was found between 2d:4d and total testosterone (TT) (r = 0.11; p < 0.05). In girls a significant association was found between 2d:4d and SHBG (r = 0.18; p < 0.01). However, relationships between 2d:4d and hormones depended on the phase of the menstrual cycle, with 2d:4d being negatively associated with FT (B = − 0.013; p < 0.05) once a positive association between 2d:4d and FT for girls in the mid-cycle group (B = 0.019; p < 0.01) is taken into account. With respect to sex differences in characteristics, we found evidence of a relationship between 2d:4d and depression in boys (r = − 0.14; p < 0.05) but not between 2d:4d and dominance, ART or NART. No relationships were found between 2d:4d and any of these variables in girls. 相似文献
5.
Renee Mercaldo-Allen Catherine Kuropat 《Journal of experimental marine biology and ecology》2006,329(2):187-195
This study presents an indirect method for estimating growth rates of young-of-the-year (YOY) tautog, Tautoga onitis, based on laboratory calibration experiments and nucleic acid-based indices. Field-collected tautog were held in the laboratory at 3 temperatures over a 17-day period. Four feeding levels were used to produce a range of growth rates. An ultraviolet absorption assay was used to measure nucleic acid concentrations in white muscle tissue. The strength of the relationship between growth rate and three nucleic acid-based parameters (RNA concentration, DNA concentration, RNA/DNA ratio (R/D)) was tested. Correlation results indicated a significant positive relationship between R/D and weight-based instantaneous growth rate (G) (r = 0.68; p < 0.001). Both R/D (r = − 0.55; p < 0.006) and RNA (r = − 0.56; p < 0.005) were highly negatively correlated with temperature (T). Multiple linear regression showed that R/D and temperature explained 61% of the variability in growth, resulting in the model G = 0.01285(R/D) + 0.00057(T) − 0.03205 (p < 0.0001). This R/D-temperature model can be used to evaluate recent growth rates in YOY tautog under field conditions and has applications for aquaculture when comparing growth rates of fish held under different culture conditions. 相似文献
6.
Marian C. Cheung Gertrud Wolfbauer Hiroshi Deguchi José A. Fernández John H. Griffin John J. Albers 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2009,1791(3):206-211
To gain further insights into the relationship between plasma phospholipid transfer protein (PLTP) and lipoprotein particles, PLTP mass and phospholipid transfer activity were measured, and their associations with the level and size of lipoprotein particles examined in 39 healthy adult subjects. No bivariate correlation was observed between PLTP activity and mass. PLTP activity was positively associated with cholesterol, triglyceride, apo B and VLDL particle level (rs = 0.40–0.56, p ≤ 0.01) while PLTP mass was positively associated with HDL-C, large HDL particles, and mean LDL and HDL particle sizes (rs = 0.44–0.52, p < 0.01). Importantly, plasma PLTP specific activity (SA) was significantly associated with specific lipoprotein classes, positively with VLDL, IDL, and small LDL particles (rs = 0.42–0.62, p ≤ 0.01) and inversely with large LDL, large HDL, and mean LDL and HDL particle size (rs = − 0.42 to − 0.70, p ≤ 0.01). After controlling for triglyceride levels, the correlation between PLTP mass or SA and HDL size remained significant. In linear models, HDL size explained 45% of the variability of plasma PLTP SA while triglyceride explained 34% of the PLTP activity. Thus, in healthy adults a significant relationship exists between HDL size and plasma PLTP SA (rs = − 0.70), implying that HDL particle size may modulate PLTP SA in the vascular compartment. 相似文献
7.
8.
To evaluate the effect of dietary and management factors on boar hormonal status during ejaculation, 39 boars were canulated to determine the profiles of luteinizing hormone (LH), follicle-stimulating hormone (FSH), 17β-estradiol (E2), and testosterone (T) in blood plasma and seminal fluid. Prior to canulation, 18 boars were fed a basal diet (control), whereas the remainder (n = 21) were fed a basal diet supplemented with extra vitamins (supplemented). Within each dietary treatment, two regimens of semen collection were used over the 3 mo preceding the hormonal evaluation: three times per 2 wk (3/2) or three times per wk (3/1). Plasma E2 was lower (P < 0.01) before ejaculation (232.5 ± 22.6 pg/mL) than at the onset of ejaculation (255.2 ± 27.1 ng/mL). Plasma T increased from 5.14 ± 0.72, before ejaculation to 5.87 ± 0.86 ng/mL at the onset of ejaculation in supplemented boars, whereas it decreased from 5.15 ± 0.65 to 4.87 ± 0.70 ng/mL in controls (diet by time, P < 0.05). At the onset of ejaculation, plasma FSH was higher in 3/2 boars (0.436 ± 0.06 ng/mL) than in 3/1 boars (0.266 ± 0.04 ng/mL; P < 0.05). During ejaculation, plasma LH increased linearly (P < 0.01) from 0.59 ± 0.07 to 0.97 ± 0.10 ng/mL, and plasma E2 and T concentrations were correlated (r = 0.62, P < 0.01). Plasma FSH before and during ejaculation was negatively correlated with sperm production (r = −0.60, P < 0.01) and testicular weight (r = −0.50, P < 0.01). In conclusion, dietary and management factors had few impacts on hormonal profiles during ejaculation, but homeostasis of some hormones was related to some criteria of reproductive performance in boars. 相似文献
9.
Mafra D Farage NE Lobo JC Stockler-Pinto MB Leal VO Carvalho DP Leite M 《Peptides》2011,32(2):358-361
In hemodialysis (HD) patients studies have shown that plasma ghrelin is increased and it has been speculated that ghrelin levels might be related to systemic inflammation. The present study attempted to correlate the serum levels of total ghrelin with serum TNF-α and IL-6, and with nutritional status and body composition in HD patients. Forty-seven HD patients from a single dialysis unit (18 women, mean age 55.3 ± 12.2 yr; BMI 24.4 ± 4.2 kg/m2; % body fat 29.4 ± 7.4%) were studied and compared to 21 healthy subjects (12 women, 50.7 ± 15.7 yr and BMI 25.6 ± 4.0 kg/m2; % body fat 30.0 ± 5.7%). Biochemical data, serum total ghrelin, TNF-α and IL-6 levels were measured. The body composition was evaluated by dual energy X-ray absortiometry (DEXA) and energy and protein intake were evaluated. Patients showed elevated plasma ghrelin levels when compared to healthy subjects (1.14 ± 1.0 ng/mL vs 0.58 ± 0.4; p < 0.001). There was a positive correlation between ghrelin levels and TNF-α (r = 0.25; p < 0.04), IL-6 (r = 0.42; p < 0.02), and a negative correlation between TNF-α and protein intake (r = −0.28; p < 0.03), and energy intake (r = −0.34; p < 0.01). No correlation was observed with any aspect of body composition. Plasma ghrelin levels are elevated in HD patients and associated with the state of systemic inflammation. We suggest that the inflammatory state may affect ghrelin bioactivity and metabolism in hemodialysis patients. 相似文献
10.
L.G.B. Siqueira C.A.A. Torres P.L.J. Monteiro Jr. L.S.A. Camargo J.H.M. Viana 《Theriogenology》2009,72(7):949-958
The objective was to investigate the influence of corpora lutea physical and functional characteristics on pregnancy rates in bovine recipients synchronized for fixed-time embryo transfer (FTET). Crossbred (Bos taurus taurus × Bos taurus indicus) nonlactating cows and heifers (n = 259) were treated with the following protocol: 2 mg estradiol benzoate (EB) plus an intravaginal progesterone device (CIDR 1.9 g progesterone; Day 0); 400 IU equine chorionic gonadotropin (eCG; Day 5); prostaglandin F2α (PGF2α) and CIDR withdrawal (Day 8); and 1 mg EB (Day 9). Ovarian ultrasonography and blood sample collections were performed on Day 17. Of the 259 cattle initially treated, 197 (76.1%) were suitable recipients; they received a single, fresh, quality grade 1 or 2 in vivo-derived (n = 90) or in vitro-produced (n = 87) embryo on Day 17. Pregnancy rates (23 d after embryo transfer) were higher for in vivo-derived embryos than for in vitro-produced embryos (58.8% vs. 31.0%, respectively; P < 0.001). Mean (±SD) plasma progesterone (P4) concentration was higher in cattle that became pregnant than that in nonpregnant cattle (5.2 ± 5.0 vs. 3.8 ± 2.4 ng/mL; P = 0.02). Mean pixel values (71.8 ± 1.3 vs. 71.2 ± 1.1) and pixel heterogeneity (14.8 ± 0.3 vs. 14.5 ± 0.5) were similar between pregnant and nonpregnant recipients (P > 0.10). No significant relationship was detected between pregnancy outcome and plasma P4, corpus luteum area, or corpus luteum echotexture. Embryo type, however, affected the odds of pregnancy. In conclusion, corpus luteum-related traits were poor predictors of pregnancy in recipients. The type of embryo, however, was a major factor affecting pregnancy outcome. 相似文献
11.
The ability to rapidly identify temporal deviations of an animal from its norm will be important in the management of individual cows in large herds. Furthermore, predictors of genetic merit for especially health traits are useful to augment the accuracy of selection, and thus genetic gain, in breeding programs. The objective of this study was to estimate the repeatability of milking order and to quantify the contribution of differences in additive genetic variation to phenotypic differences (i.e., heritability). The data used in this study included 9813 herd milk recording test-day records with time of milking from 85,532 cows in 1143 herds across an 8-year period. Milking order was available for both morning and evening milking for each cow with, on average, 3.33 milk test-day records (i.e., 6.66 milking events) per lactation, and on average 1.62 lactations per cow. Variance components for milking order were estimated using animal linear mixed models; covariance components between milking order and milk yield, milk composition and somatic cell score (i.e., logarithm10 somatic cell count) were estimated also using animal linear mixed models. The heritability of milking order was 0.20 indicating partial genetic control of milking order. The repeatability of milking order within test-day, within lactation, and across lactations was 0.63, 0.51, and 0.47, respectively. Milking order was positively (P < 0.001), but weakly, phenotypically correlated with milk yield (r = 0.04), and milk fat concentration (r = 0.01) and negatively (P < 0.001), but weakly, correlated with milk protein concentration (r = −0.02) and somatic cell score (r = −0.05). Milking order was positively (P < 0.05), although weakly, genetically correlated with milk yield (r = 0.07) and negatively (P < 0.05), but also weakly, genetically correlated with somatic cell score (r = −0.08). This study is the first to show a contribution of additive genetics to milking order in dairy cattle but the genetic correlation between milking order and somatic cell score was weak. 相似文献
12.
Que-Kun Peng Gao-Cao Wang Dong Yang Bi-Song Yue Li Li Fang-Dong Zou 《Biochemical Systematics and Ecology》2010
The black-spotted tokay gecko and red-spotted tokay gecko have different distribution areas and are significantly different in appearance but are classified into the same species Gekko gecko. Twelve microsatellite loci were isolated, characterized and evaluated from wild black-spotted tokay geckos for the first time. Of them, nine loci were successfully amplified in red-spotted tokay geckos using multiplex polymerase chain reactions (PCRs). A total of 208 different alleles were observed in the 70 wild black-spotted and red-spotted tokays, and the average number of alleles per locus was 17.3. The average values for observed heterozygosity, expected heterozygosity and polymorphism information content were 0.762, 0.891 and 0.871, respectively, which showed that the wild G. gecko population had a high level of genetic variability. Both black-spotted tokays and red-spotted tokays showed a significant (P < 0.001) deficit of heterozygotes. The red-spotted tokay (HE = 0.881, A = 16.4) had a higher level of genetic variability than black-spotted tokay (HE = 0.804, A = 10.7). The pairwise FST (P < 0.001) estimates of the two types of tokay were 0.143, which indicated that there was a significant level of genetic differentiation between the two. 相似文献
13.
《Microbes and infection / Institut Pasteur》2020,22(10):622-625
COVID-19 patients (n = 114) were included (55 patients with pneumonia (group P) and 59 without pneumonia (group NP). Patients in group P were older (69 (±17) years vs 46 (±16); p < 0.001) with a male predominance (58.2% vs 27.1%; p < 0.001). The symptoms which were statistically more frequents in patients with pneumonia were fever ≥ 38 °C (93% vs 70%; p = 0.002) and dyspnea (73% vs 22%; p < 0.001). Symptoms such as facial headache (42% vs 15%; p = 0.001), sore throat (39% vs 16%; p = 0.007), dysgeusia (61% vs 33%; p = 0.003), anosmia (63% vs 31%; p = 0.001) were statistically more frequents in patients without pneumonia. 相似文献
14.
Piesik D Wenda-Piesik A Kotwica K Łyszczarz A Delaney KJ 《Journal of plant physiology》2011,168(17):2134-2138
We report large induction (>65fold increases) of volatile organic compounds (VOCs) emitted from a single leaf of the invasive weed mossy sorrel, Rumex confertus Willd. (Polygonaceae), by herbivory of the dock leaf beetle, Gastrophysa polygoni L. (Coleoptera: Chrysomelidae). The R. confertus VOC blend induced by G. polygoni herbivory included two green leaf volatiles ((Z)-3-hexenal, (Z)-3-hexen-1-yl acetate) and three terpenes (linalool, ß-caryophyllene, (E)-ß-farnesene). Uninjured leaves produced small constitutive amounts of the GLVs and barely detectable amounts of the terpenes. A Y-tube olfactometer bioassay revealed that both sexes of adult G. polygoni were attracted to (Z)-3-hexenal and (Z)-3-hexen-1-yl acetate at a concentration of 300 ng h−1. No significant G. polygoni attraction or repellence was detected for any VOC at other concentrations (60 and 1500 ng h−1). Yet, G. polygoni males and females were significantly repelled by (or avoided) at the highest test concentration (7500 ng h−1) of both GLVs and (E)-ß-farnesene. Mated male and female G. polygoni might be attracted to injured R. confertus leaves, but might avoid R. confertus when VOC concentrations (especially the terpene (E)-ß-farnesene) suggest high overall plant injury from conspecifics, G. viridula, or high infestations of other herbivores that release (E)-ß-farnesene (e.g., aphids). Tests in the future will need to examine G. polygoni responses to VOCs emitted directly from uninjured (constitutive) and injured (induced) R. confertus, and examine whether R. confertus VOC induction concentrations increase with greater tissue removal on a single leaf and/or the number of leaves with feeding injury. 相似文献
15.
Buthbumrung N Mahidol C Navasumrit P Promvijit J Hunsonti P Autrup H Ruchirawat M 《Chemico-biological interactions》2008,172(3):185-194
Traffic related urban air pollution is a major environmental health problem in many large cities. Children living in urban areas are exposed to benzene and other toxic pollutants simultaneously on a regular basis. Assessment of benzene exposure and oxidative DNA damage in schoolchildren in Bangkok compared with the rural schoolchildren was studied through the use of biomarkers.Benzene levels in ambient air at the roadside adjacent to Bangkok schools was 3.95-fold greater than that of rural school areas. Personal exposure to benzene in Bangkok schoolchildren was 3.04-fold higher than that in the rural schoolchildren. Blood benzene, urinary benzene and urinary muconic acid (MA) levels were significantly higher in the Bangkok schoolchildren. A significantly higher level of 8-hydroxy-2′-deoxyguanosine (8-OHdG) in leukocytes and in urine was found in Bangkok children than in the rural children. There was a significant correlation between individual benzene exposure level and blood benzene (rs = 0.193, P < 0.05), urinary benzene (rs = 0.298, P < 0.05), urinary MA (rs = 0.348, P < 0.01), and 8-OHdG in leukocyte (rs = 0.130, P < 0.05). In addition, a significant correlation between urinary MA and 8-OHdG in leukocytes (rs = 0.241, P < 0.05) was also found. Polymorphisms of various xenobiotic metabolizing genes responsible for susceptibility to benzene toxicity have been studied; however only the GSTM1 genotypes had a significant effect on urinary MA excretion.Our data indicates that children living in the areas of high traffic density are exposed to a higher level of benzene than those living in rural areas. Exposure to higher level of benzene in urban children may contribute to oxidative DNA damage, suggesting an increased health risk from traffic benzene emission. 相似文献
16.
Sadaruddin Biswas 《HOMO》2010,61(4):271-276
One of the greatest problems facing developing countries, including rural India, is undernutrition in terms of stunting among under 5-year-old children. However, there exists scanty information on the prevalence of stunting among preschool children in India and in particular in West Bengal. This study investigated prevalence of stunting and identified the predictor(s) of stunting among 1-5-year-old Bengalee rural preschool children of Integrated Child Development Services (ICDS) centres. This cross-sectional study was undertaken at different ICDS centres of Chapra Block, Nadia District, West Bengal, India. A total of 673 preschool children (323 boys and 350 girls), aged 1-5 years were selected from 30 randomly selected ICDS centres to study the impact of parents’ educational status and child birth order on stunting. The overall (age and sex combined) rate of stunting was 39.2%. Child birth order (BO) (χ2 = 14.10, df = 1, p < 0.001), father educational status (FES) (χ2 = 21.11, p < 0.001) and mother educational status (MES) (χ2 = 14.34, df = 1, p > 0.001) were significantly associated with the prevalence of stunting among girls. Logistic regression analyses revealed that both FES (Wald = 19.97, p < 0.001) as well as MES (Wald = 13.95, p < 0.001) were strong predictors of stunting among girls. Similarly BO (Wald = 13.71, p < 0.001) was a strong predictor of stunting among girls. Girls with ≥3rd BO had significantly higher risk (OR = 2.49, CI = 1.54-4.03) of stunting than those with ≤2nd BO. Moreover, girls with FES lower than secondary level had significantly (OR = 3.30, CI = 1.96-5.58) higher rate of stunting than those with FES ≥ secondary level. Similarly, girls with MES < secondary level had significantly (OR = 2.50, CI = 1.54-4.03) higher rate of stunting than those with FES ≥ secondary level.In conclusion our study revealed that BO as well as parents’ educational status were strong predictors of stunting among girls but not boys. Sex discrimination could be a likely cause for this sex difference in the impact of BO and parents’ educational status. 相似文献
17.
In entomopathogenic hyphomycetes, desired candidates against the brown planthopper, Nilaparvata lugens (a sap-sucking rice pest in Asia), are lacking. In this study, 21 Beauveria bassiana isolates from heterologous host insects showed low pathogenicity to third-instar nymphs sprayed at the high concentration of ∼1000 conidia/mm2, causing only 2-23% mortalities. Of those, three isolates killed significantly more nymphs (up to 45-62%) after two in vivo passages but no more after further passage. Conidial hydrophobicity rates (Hr), zeta potentials (Pz), and subtilisin-like protease (Pr1) activities (Ap) of these isolates showed the same trends in the three host passages (N: 0-3). In multivariate correlation, the variables N, Hr and Pz were found contributing 89% to the mortality variation (r2 = 0.89). Significant positive correlations were also found between Hr and N (r2 = 0.64), Pz and N (r2 = 0.52), Ap and N (r2 = 0.51), Hr and Ap (r2 = 0.45), and Pz and Ap (r2 = 0.57), respectively. However, irregular changes of Hr and Pz occurred in four other isolates, whose pathogenicity to N. lugens was not enhanced by repeated host passages, resulting in no correlation between the variables. Our data indicate that the conidial surface properties Hr and Pz associated with cuticle adhesion reflect the heterologous host-induced adaptation and help to select fungal candidates against N. lugens from repeated in vivo passages. 相似文献
18.
Lúcia Alves da Silva Lara Alfredo Ribeiro da Silva Julio Cesar Rosa-e-Silva Marcos Felipe Silva-de-Sá Ana Carolina Japur de Sá Rosa-e-Silva 《Tissue & cell》2014
After menopause, critically estrogen low levels result in modifications in vaginal wall. This cross-sectional study aims to determine whether there is a change in the number of vessels in the lamina propria of the vagina after menopause in parallel to the ER-alpha expression on the vaginal wall. Twelve women who underwent a genital surgery for genital prolapse up to grade II were selected. They were divided into two groups: a premenopausal group (PG) consisting of six women who were 18–40 years old with FSH levels =12 mIU/ml and regular cycles, and a menopausal group (MG) consisting of six women at least one year after menopause who were <65 years old with FSH levels =40 mIU/ml. Slides were stained for ER-alpha immunohistochemistry, and an endothelial cell marker CD3 was used to label vessels which were identified by using a system for morphometry. The number of vessels was significantly higher in the PG than in the MG both on the anterior wall (PG: 1.055 ± 145.8 vessels/mm2, MG: 346.6 ± 209.9 vessels/mm2, p < 0.0001) and on the posterior wall (PG: 1064 ± 303.3 vessels/mm2, MG: 348.6 ± 167.3 vessels/mm2, p = 0.0005). The ER-alpha score was significantly higher in the PG than the score for the MG on both the anterior and posterior walls (PG: 6.0 ± 0.52, MG: 2.5 ± 0.89, p = 0.007; PG: 5.8 ± 0.79, MG: 2.7 ± 0.95, p = 0.03, respectively). There was a positive correlation between the ER-alpha score and the vessel concentration on the anterior (r = 0.6656, p = 0.018) and posterior (r = 0.6738, p = 0.016) vaginal walls. Age was strongly negatively correlated with vessel concentration on the vaginal walls (respectively r = -0.9033, p < 0.0001, r = -0.7440, p = 0.0055). Therefore, postmenopausal women with genital prolapse have a smaller number of vessels on the vaginal wall compared to normoestrogenic controls with the same pathological condition. Hypoestrogenism and advancing age are factors that are associated to these changes. 相似文献
19.
Dimensions of oospores such as length, width, fossa-breadth, length-to-width ratio and number of striae were considered for morphological identification of the members of Charales. Oospores were collected from natural stands of three species, viz. Chara braunii, C. corallina and C. wallichii in ponds in West Bengal (India). Oospore dimensions (length, width and fossa-breadth) correlated well: though the fossa breadth–length relation of C. braunii showed lower correlation coefficient (r2 = 0.076, p < 0.001), all other five breadth or width–length relationships showed r2 ≥ 0.23, p < 0.001. These bivariate patterns were significantly different among the species (one-way ANCOVA of length–width ratio to fossa-breadth: adjusted means and slopes both p < 0.001), C. braunii and C. corallina has similar length to width ratios, C. wallichii and C. corallina were similar in width, whereas the species differed in breadth and length in the order C. braunii, C. wallichii, and C. corallina. 相似文献
20.
Evgeny Krynetskiy Natalia Krynetskaia Diana Rihawi Katarzyna Wieczerzak Victoria Ciummo Ellen Walker 《Life sciences》2013