共查询到20条相似文献,搜索用时 46 毫秒
1.
Microphotometric measurements of fast reacting protein thiols (PSHr) and proteins were performed on freshly frozen sections of samples from normal skin (26 cases as controls) and from 45 basal cell epitheliomas (basalioma; BCE). The intensity of the staining (E/micron2) for both proteins and PSHr was significantly higher in normal epidermis than in the adjacent dermis. The values of QE (quotient of values observed in the epidermis divided by those observed in the dermis) were calculated to be 3.48 for proteins (QE, Prot) and 4.62 for PSHr (QE, PSHr). In cases of BCE, significantly lower QE values were found: QE, Prot = 2.16 and QE, PSHr = 1.72. The decrease of QE, PSHr was due to a decrease in the staining intensity observed in the BCEs, whereas practically no changes occurred in the adjacent dermis. The decrease of QE, Prot was mainly caused by a decrease in the staining intensity in the BCE (by 68%) as well as in the adjacent dermis (by 36%). By dividing the mean extinction value (E/micron2) for PSHr by the E/micron2 for proteins, a new quotient, PSHr/Prot, is obtained which can serve as a quantitative measure of the content of the tissue proteins of PSHr. The proteins of normal epidermis contained more PSHr than dermal proteins. The proteins of BCEs also contained more PSHr than those of the adjacent dermis, but the PSHr/Prot values of both tissues were 1.5 to 1.6 times greater than the corresponding values for normal epidermis and dermis, respectively.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
2.
Ray I. Chauhan A. Wisniewski H. M. Wegiel J. Kim K. S. Chauhan V. P. S. 《Neurochemical research》1998,23(10):1277-1282
Amyloid beta-protein (A), in its soluble form, is known to bind several circulatory proteins such as apolipoprotein (apo) E, apo J and transthyretin. However, the binding of A to intracellular proteins has not been studied. We have developed an overlay assay to study A binding to intracellular brain proteins. The supernatants from both rat and human brains were found to contain several proteins that bind to A 1–40 and A 1–42. No major difference was observed in the A binding-proteins from brain supernatants of patients with Alzheimer's disease and normal age-matched controls. Binding studies using shorter amyloid beta-peptides and competitive overlay assays showed that the binding site of A to brain proteins resides between 12–28 amino acid sequence of A. The presence of several intracellular A-binding (AB) proteins suggests that these proteins may either protect A from its fibrillization or alternatively promote A polymerization. Identification of these proteins and their binding affinities for A are needed to assess their potential role in the pathogenesis of Alzheimer's disease. 相似文献
3.
Sergei Kan 《Dialectical Anthropology》1989,14(2):81-94
Conclusion Even a cursory look at the ethnographic literature on other Northwest Coast societies reveals some striking similarities with the Tlingit way of conceptualizing aristocrats as special persons. Thus the Kwakiutl referred to their chiefs as real or complete people, who were heavier than commoners. The Coast Tsimshian called their highest aristocrats real or ripe persons, in contrast to the low-ranking ones, who were described as unhealed or green. The Coast Tshimshian also referred to their chiefs as strong, heavy, and solid like a rock. The neighboring Gitksan contrasted the chiefs, described as people who were good and clean and stayed put, with the commoners, who were said to be dirty, ignorant, and always moving around. Because spirits of the dead liked to return to persons who were clean and showed respect by giving away wealth and feasts, there was considerable moral and practical pressure on the aristocrats to remain pure, train knowledgeable and clean heirs, and continue potlatching. Finally, among the Haida, rank was tied to a wider system of symbolic classification, associating aspects of food, space, clothing, ritual pollution and the ethic of industry with attributes of seniority.While some of the symbolic associations of aristocratic status are culture specific, others are present in several, if not all, of the NWC cultures. What we need is a comparative symbology of aristocratic status, which would combine the reanalysis of the existing ethnographic data with the introduction of some new materials that can still be obtained in the field. Such work would be the best tribute to Irving Goldman himself and to our common illustrious ancestors—Franz Boas and Marcel Mauss.Sergei Kan is Professor of Anthropology at Dartmouth College. 相似文献
4.
Summary The absence of the methyl substituent at the 2position of the cyclohexene ring of TCHP enhances the conversion rate as well as the yields of the 3-hydroxy product obtained byStreptomyces natalensis and the 3-keto product obtained byMycobacterium smegmatis.Abbreviations TCHP
1-(2-thienyl)-3-(1-cyclohexen-1-yl)-1-propanone
- TCHP-OH
1-(2-thienyl)-3-(3-hydroxyl-1-cyclohexen-1-yl)-1-propanone
- TCHP-ketone
1-(2-thienyl)-3-(1-cyclohexen-1-yl-3-one)-1-propane
- TMCHP
1-(2-thienyl)-3-(2-methyl-1-cyclohexen-1-yl)-propanone 相似文献
5.
Immature embryo rescue,culture and seedling development of acid citrus fruit derived from interploid hybridization 总被引:1,自引:0,他引:1
Interploid sexual hybridizations were completed in 2001 and 2002 between seven lemon (Citrus limon(L.) Burm. f.) varieties, Key lime (C. aurantifolia (Cristm.) Swing), Palestine sweet lime (C. imettioides Tan.), Lakeland limequat (C. aurantifolia x Fortunella japonica (Thumb.) Swing.), and Etrog citron (C. medica L.) as diploid progenitors and four allotetraploid somatic hybrids (Key lime + Valencia orange, Hamlin orange + Femminello lemon, Valencia orange + Rough lemon, and Valencia orange+ Femminello lemon) in efforts to generate improved seedless triploid acid fruit hybrids. Efficient recovery of triploid progeny from such crosses requires embryo rescue to avoid embryo abortion due to endosperm failure. Germination of rescued genetically diverse immature embryos was induced on two culture media (EME and Gamborgs B5), with two sucrose concentrations (50 or 70 g l–1). All media contained 0.5 g l–1 malt extract and 4.50 M GA3. Germination of globular, heart and torpedo shaped embryos (defined as small embryos) was significantly (p < 0.05) affected by medium and genotype. Gamborgs medium induced 82.89% germination. Of germinated embryos, 11–65% developed into normal plants with differences among crosses. Cotyledonary embryos (defined as immature embryos with fully developed cotyledons) germinated and developed into normal plants at higher rates than less-developed embryos. In efforts to improve the efficiency of plant recovery, small embryos from Todo el año × HF and Lisbon × HF crosses conducted during 2002 were rescued and cultured on three media (MS, Gamborgs, and RMA) for comparison. Media did not significantly affect the proportion of normal plant recovery. 相似文献
6.
Brian P. Lockhart Thomas P. Smith Christopher J. Bailey 《The Histochemical journal》1991,23(9):385-391
Summary An epidermolytic toxin target was observed in keratohyalin granules of sectioned epidermis by a direct fluorescence procedure using FTC-toxin, but not by an indirect procedure using sequential reaction with toxin, anti-toxin and FTC-secondary antibody. The investigation of the two procedures was extended to keratinocytes. A dispase digestion procedure yielded three fractions which corresponded to basal, spinous and granular cells according to biochemical and morphological criteria. It was shown that the direct and indirect procedures both detected the toxin target in the keratohyalin granules of granular cells, but that the indirect procedure was very insensitive. In control experiments, the profilaggrin of keratohyalin granules was detected readily in cells by a direct procedure using FTC-antiprofilaggrin but only weakly by an indirect double antibody procedure. Insensitivity to indirect procedures thus appears to be a particular property of the keratohyalin granule site. It was shown that the toxin target was readily accessible in permeable (trypsin-isolated) granular cells but inaccessible in impermeable (dispase-isolated) cells. 相似文献
7.
Wilhelm Schmitz Peter Boknik Bettina Linck Frank U. Müller 《Molecular and cellular biochemistry》1996,157(1-2):251-258
In end-stage heart failure the expression of different myocardial regulatory proteins involved in the -adrenergic cAMP signalling pathway is altered. The downregulation of -adrenoceptors and their uncoupling from the effector as well as an increased expression of the inhibitory GTP-binding protein seem to be the most important alterations. Since catecholamine levels are elevated in these patients and since some alterations can be restored after treatment with -adrenoceptor antagonists it was hypothesized that excessive -adrenergic stimulation could be involved in these alterations.In this article the changes of -adrenergic receptors, GTP-binding proteins, sarcoplasmic reticulum Ca2+-ATPase and of phospholamban found in heart failure are addressed with its possible therapeutic implications. 相似文献
8.
Marita Bengtsson Johan Broddefalk Jan Dahmen Krister Henriksson Jan Kihlberg Hans Lonn B.R. Srinivasa Kristina Stenvall 《Glycoconjugate journal》1998,15(3):223-231
The 2-bromoethyl -glycosides of the disaccharide galabiose [Gal(1-4)Gal] and the trisaccharides globotriose [Gal(1-4)Gal(1-4)Glc] and 3-sialyllactose [Neu5Ac(2-3)Gal(1-4)Glc] have been prepared by improved routes. The 2-bromoethyl glycosides were then used in cesium carbonate promoted alkylations of the sulfhydryl groups of cysteine and homocysteine residues in T cell stimulating peptides. This convergent and general approach was used to prepare 16 neoglycopeptides which were obtained in 52–95% yields after purification by HPLC. 1H NMR spectroscopy revealed that -elimination and epimerization of neoglycopeptide stereocentres did not occur during the synthesis. 相似文献
9.
Summary The purified bacteriophage replication proteins O and P sediment separately in metrizamide gradients of low ionic strength as dimers. Together they interact with each other forming an oligomer, composed of two molecules of O and one molecule of P. The O-P oligomer is active in the in vitro replication of ori-containing DNA.Equilibrium sedimentation in preformed metrizamide density gradients under conditions that separate DNA-protein complexes from free proteins was employed in order to study possible interactions among the replication proteins and ori DNA. It was found that the P protein binds specifically to ori-containing plasmid DNA only in the presence of O protein. About 100 molecules of O and 10 molecules of P form a complex with the ori DNA. The DNA-O-P complex was shown to be active in an in vitro replication system.Since the physical interactions between ori and O and between P and the Escherichia coli dnaB replication protein are well documented, the evidence for a O-P interaction presented in this paper provides the missing link in the molecular mechanism that enables to direct the host replication machinery to the replication of its own DNA. 相似文献
10.
Although a -turn consists of only four amino acids, it assumes many different types in proteins. Is this basically dependent on the tetrapeptide sequence alone or is it due to a variety of interactions with the other part of a protein? To answer this question, a residue-coupled model is proposed that can reflect the sequence-coupling effect for a tetrapeptide in not only a -turn or non--turn, but also different types of a -turn. The predicted results by the model for 6022 tetrapeptides indicate that the rates of correct prediction for -turn types I, I, II, II, VI, and VIII and non--turns are 68.54%, 93.60%, 85.19%, 97.75%, 100%, 88.75%, and 61.02%, respectively. Each of these seven rates is significantly higher than
= 14.29%, the completely randomized rate, implying that the formation of different -turn types or non--turns is considerably correlated with the sequences of a tetrapeptide. 相似文献
11.
High frequency of triplicated α-globin loci and absence or low frequency of α thalassemia in Polynesian Samoans 总被引:3,自引:0,他引:3
Summary Most of the population in certain areas of Melanesia have one -globin gene deletion ( thal2). It is thought that the high frequencies of thal2 in this population is due to a selective advantage given by malaria infection to carriers of thal2. We are interested in neighboring Polynesia which, although adjacent to Melanesia, has always been free of malaria due to the absence of the vector anopheles. We studied 60 Polynesian Samoans and 150 Malaysians by restriction endonuclease gene mapping using Eco RI, Bam HI, and Bgl II and hybridization to 32P-labeled -globin gene probe. Seven among the 60 (11.7%) Samoans had triplicated -globin loci type 1, while none had thal2. On digestion with Bgl II the third -globin gene was found in an additional 3.7kb fragment in all seven Samoans with triplicated -globin loci, while digestion with Bam HI produced an abnormal elongated 18.2 kb fragment carrying -globin genes in addition to the normal 14.5 kb fragment. None of the Polynesian Samoans had thal2 or thal1. Only two of the Malaysians had triplicated -globin loci. 相似文献
12.
The relative proportions of -helix, -sheet, and unordered form in -lactoglobulin A and B were examined in solutions of urea, guanidine, and sodium dodecyl sulfate (SDS). In the curve-fitting method of circular dichroism (CD) spectra, the reference spectra of the corresponding structures determined by Chen et al. (1974) were modified essentially according to the secondary structure of -lactoglobulin B predicted by Creamer et al. (1983), i.e., that the protein has 17% -helix and 41% -sheet. The two variants showed no appreciable difference in structural changes. The reduction of disulfide bridges in the proteins increased -sheet up to 48% but did not affect the -helical proportion. The -helical proportions of nonreduced -lactoglobulin A and B were not affected below 2 M guanidine or below 3 M urea, but those of the reduced proteins began to decrease in much lower concentrations of these denaturants. By contrast, the -helical proportions of the nonreduced and reduced proteins increased to 40–44% in SDS. The -sheet proportions of both nonreduced and reduced proteins, which remained unaffected even in 6 M guanidine and 9 M urea, decreased to 24–25% in SDS. 相似文献
13.
Dr. rer. nat. Peter Wenk 《Zoomorphology》1965,55(6):656-670
Zusammenfassung Außer an ihren Blutwirten findet man die bei uns heimischen Simuliiden regelmäßig im Frühjahr auf der Weißweide (Salix alba), im Sommer auf den gelbgrünen Blüten und Früchten des Pastinak (Pastinca sativa) und im Herbst auf den grünen Blüten des Efeu (Hedera helix). Die Blüten zumindest von Pastinak und Efeu werden von allen Simuliidenarten selektiv angeflogen. Die übrigen Umbelliferen bleiben, auch wenn sie in unmittelbarer Nähe von Pastinak wachsen, unberührt. Efeu wird auch dann reichlich angeflogen, wenn er in einzelnen Stöcken an entlegenen Plätzen wächst. Alle von Simuhiden nachweislich direkt angeflogenen Blüten haben eine in der Natur unauffällige, entweder gelbe, gelbgrüne oder rein grüne Farbe und offene Nektarien. Aus diesen Gründen scheint mindestens beim Anflug auf Efeu und Pastinak eine olfaktorisch gesteuerte Orientierung vorzuliegen. Sie ist offenbar viel spezifischer als die vorwiegend optische Orientierung derselben Simuliidenarten nach ihren Blutwirten.Durchschnittlich sind etwa doppelt soviele wie auf Blüten anzutreffen. Abends und nachts sowie an kalten, regnerischen Tagen findet man mehr Simuliiden auf Pastinak als an sonnigen Tagen. Die auf Blüten angetroffenen der mammalophilen Simuliidenarten Wilhelmia salopiensis, W. equina, Boophthora erythrocephala und Odagmia ornata sind durchschnittlich zu 90% besamt, die der ornithophilen Art Eusimulium latipes nur zu 32%. Bei allen Arten fanden sich auf Blüten stets auch einige mit Blut im Mitteldarm oder legereifen Eiern im Abdomen. Der Blütenbesuch kann demnach vor und nach dem Blutsaugen stattfinden.Beim Anflug auf den Blutwirt sind die der genannten mammalophilen Arten ebenfalls zu rund 98% besamt, die von E. latipes wiederum nur zu 53%. Das Verhalten dieser Art, welche auch nachts Blut saugt, scheint von dem der mammalophilen Arten hinsichtlich der Geschlechterfindung erheblich abzuweichen.Das bekannte tagesperiodische Anflugsverhalten der auf ihren Säugetierblutwirt mit je einem Maximum nach Sonnenaufgang und vor Sonnenuntergang läßt sich auch bei den zugehörigen feststellen, wean man berücksichtigt, daß die den Blutwirt nicht direkt anfliegen, sondern ihn nur umschwärmen.Das Anflugsverhalten der und ist von der Änderung der Lichtintensität in der Zeiteinheit (Wolfe u. Peterson 1960), das Schwarmverhalten der allein von der absoluten Tageshelligkeit (Wenk 1965b) abhängig. Die Kopulation kann demnach einerseits morgens und abends in der Nähe des Blutwirtes und andererseits tagsüber beim Schwärmen der stafffinden. Wie wir später direkt zeigen konnten, sind beide Arten der Geschlechterfindung zur Kopulation bei drei von den vier genannten mammalophilen Simuliidenarten verwirklicht (Wenk 1965b). Die Kopulation von O. ornata und der ornithophilen Art E. latipes konnten wir dagegen selbst nie beobachten.
Summary Simuliids of South-Western Germany show a marked preference for the white willow (Salix alba) in spring, the yellow blossoms of parsnip (Pastinaca sativa) in summer, and the green blossoms of ivy (Hedera helix) in autumn, whereas all the other Umbelliferae are disregarded, although they may be numerous and in close proximity. Ivy will be found by many simuliids even if growing in single plants at hidden places. All flowers for which a predelection by simuliids can be clearly established are inconspicuous under natural conditions — being of yellow, yellowish green or pure green colour — and their nectaries are of the open type. Therefore orientation of these simuliids to their nectar donors seems to rely on olfactoric senses and is apparantly much more specific than optical orientation to the blood hosts of the same species.On average, about twice as many are found on blossoms than During the evening and night; as well as on cold rainy days, more simuliids are encountered on parsnip than on sunny days. The of the mammalophilic species Wilhelmia salopiensis, W. equina, Boophthora erythrocephala and Odagmia ornata are inseminated at the rate of 90% , the of the ornithophilic species Eusimulium latipes at 32% only. In all species, some have been found with blood in the midgut or eggs in the abdomen, i. e. visiting of flowers may take place before or after bloodsucking.All of mammalophilic species attacking their blood hosts are inseminated at the rate of 98%, the of the ornithophilic species E. latipes at 53% only. The behaviour of the latter, which is bloodsucking also at nighttime, seems to differ considerably with regard to sexfinding orientation from the mammalophilic species.The diurnal periodicity of mammalophilic in attacking the host —with the well-known maxima at sunrise and sunset — is the same for the , bearing in mind that do not attack the hosts of their directly but are flying around them. Consequently, copulation may take place either in the morning and evening near the blood host, or during the day in the swarms of . It has been demonstrated for three of the four mammalophilic species mentioned (WENK, 1965b) that sex-finding and copulation occur at both occasions.相似文献
14.
The continuum of pure and hybrid myosin heavy chain-based fibre types in rat skeletal muscle 总被引:3,自引:0,他引:3
The myosin heavy chain (MHC)-based fibre composition of adult rat adductor magnus (AM) and tibialis anterior (TA) muscles was investigated using single fibre analysis. Microelectrophoresis performed on single fibre fragments demonstrated a predominance of pure fast MHC-based fibre types (expressing only one fast MHC). Most of the fibres analysed from both the AM (72%) and TA (50%) were pure type IIB (expressing only MHCIIb). Pure type IID fibres (expressing only MHCIId) were also abundant in AM (20%) and TA (18%). In addition, hybrid fibres coexpressing MHCIIb and MHCIId in varying proportions (fibre types IIBD and IIDB) were found, as well as fibres coexpressing MHCIId and MHCIIa with a predominance of MHCIId (type IIDA) and some C fibres (coexpressing MHCI and MHCIIa in varying proportions). Considered altogether, these data reflect the dynamic nature of adult skeletal muscle fibres and indicate a continuum of MHC-based fibre types in normal rat muscle with transitions in the order IIB IIBD IIDB IID IIDA IIAD IIA IIC IC I. 相似文献
15.
Summary On t.l.c. plates 125I-cholera toxin binds to a disialoganglioside tentatively identified as GDlb with about 10 times less capacity than to ganglioside GM1. Binding of labeled toxin to both gangliosides was abolished in presence of excess amounts of unlabeled B subunit. Ganglioside extracts from human or pig intestinal mucosa showed toxin binding to gangliosides GM1 and GD1b. In ganglioside-containing lipid monolayers the penetration of the toxin was independent of the ganglioside binding capacity.Abbreviations GM2
Gal-NAc14Gal(3-2NeuAc)14G1c1Cer
- GM1
Gal3Ga1-NAc14Gal(32NeuAc)14G1c11Cer
- GD1a
NeuAc23Ga113Gal-NAc14Gal(32NeuAc)14G1c11Cer
- GD1b
Gall3Gal-NAcl4Gal(32NeuAc82NeuAc)14Glc11Cer
- GT1b
NeuAc23Ga113Ga1-NAcal4Gal(3-2NeuAc82NeuAc)14G1c11Cer
- dpPC
1,2-hexadecanoyl-sn-glycero-3-phosphocholine
- dpPE
1,2-hexadecanoyl-sn-glycero-3-phosphoethanolamine 相似文献
16.
M. K. Banerjee M. K. Kalloo 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,73(5):707-710
Summary One hundred and twenty-two varieties, lines and wild accessions of Lycopersicon were screened under three different regimes during the autumn/winter season of 1982–83 and 1983–84 for resistance to tomato leaf curl virus (TLCV). L. hirsutum f. glabratum (B6013) and L. hirsutum f. typicum (A1904) proved to be highly resistant to TLCV in all three environments. Various accessions of L. peruvianum were also highly resistant. L. pimpinellifolium (A1921) exhibited no TLCV symptoms within 90 days. Of the cultivated varieties, Acc 99 exhibited the minimim score for susceptibility; AC 142, Collection No. 2, Kalyanpur Angurlata and HS 101 had a low rating for virus incidence. The inheritance of resistance was studied in the interspecific crosses between a TLCV resistant line of L. pimpinellifolium (A1921) and five (HS 101, HS 102, HS 110, Pusa Ruby and Punjab Chhuhara) susceptible cultivars of L. esculentum. Parents, F1, F2 and backcross progenies were artificially inoculated with local strains of TLCV using vector the viruliferious whitefly, Bemisia tabaci (Genn.). Data indicated that the resistance of L. pimpinellifolium (A 1921) is monogenic and incompletely dominant over susceptibility. 相似文献
17.
Kozlov G Ekiel I Beglova N Yee A Dharamsi A Engel A Siddiqui N Nong A Gehring K 《Journal of biomolecular NMR》2000,17(3):187-194
The tertiary fold of the elongation factor, aEF-1, from Methanobacterium thermoautotrophicum was determined in a high-throughput fashion using a minimal set of NMR experiments. NMR secondary structure prediction, deuterium exchange experiments and the analysis of chemical shift perturbations were combined to identify the protein fold as an alpha-beta sandwich typical of many RNA binding proteins including EF-G. Following resolution of the tertiary fold, a high resolution structure of aEF-1 was determined using heteronuclear and homonuclear NMR experiments and a semi-automated NOESY assignment strategy. Analysis of the aEF-1 structure revealed close similarity to its human analogue, eEF-1. In agreement with studies on EF-Ts and human EF-1, a functional mechanism for nucleotide exchange is proposed wherein Phe46 on an exposed loop acts as a lever to eject GDP from the associated elongation factor G-protein, aEF-1. aEF-1 was also found to bind calcium in the groove between helix 2 and strand 4. This novel feature was not observed previously and may serve a structural function related to protein stability or may play a functional role in archaeal protein translation. 相似文献
18.
The major storage protein of jackbean (Canavalia ensiformis) has been purified by a protocol involving ammonium-sulphate precipitation, gel filtration and ion-exchange chromatography. The protein was shown by partial amino-acid-sequence data to be homologous to vicilin, a major storage protein of pea (Pisum sativum), and is thus a member of the family of legume 7S proteins exemplified by pea vicilin. This protein is thus referred to as jack-bean vicilin rather than canavalin or precanavalin as previously used. Other properties of the jack-bean vicilin (e.g. subunit relative molecular mass (Mr) and structure, resistance to proteolysis) show similarity to phaseolin, the major 7S storage protein ofPhaseolus vulgaris. Jack-bean vicilin contained no detectable -mannosidase activity, either as isolated from mature or germinating seeds, or after proteolytic treatment. -Mannosidase was also purified from jack beans, and was shown to have a subunit Mr of approx. 120,000; it was separated completely from jack-bean vicilin by a similar protocol to that used for purifying the latter. The -mannosidase was proteolytically cleaved after seed germination, but did not give polypeptides of the same Mr as jackbean vicilin. It was concluded that -mannosidase and jack-bean vicilin are not related proteins.Abbreviations DE
diethylaminoethyl
- M
relative molecular mass
- SDS
sodium dodecyl sulphate
- PAGE
polyacrylamide-gel electrophoresis 相似文献
19.
The inhibition of photosynthetic electron transport and the activity of photosynthetic carbon reduction cycle (PCR) enzymes under long-term water stress after slow dehydration was studied in non-nodulated Casuarina equisetifolia Forst. & Forst. plants. Initially, drought increased the fraction of closed Photosystem II (PS II) reaction centres (lowered qP) and decreased the quantum yield of PS II electron transport (PSII) with no enhancement of non-radiative dissipation of light energy (qN) because it increased the efficiency of electron capture by open PS II centres (Fv/Fm). As drought progressed, Fv/Fm fell and the decrease in PSII was associated with an increased qN. The kinetics of dark relaxation of fluorescence quenching pointed to an increase in a slowly-relaxing component under drought, in association with increased contents of zeaxanthin and antheraxanthin. Total NADP-dependent malate dehydrogenase activity increased and total stromal fructose-1,6-bisphosphatase activity decreased under drought, while the activation state of these enzymes remained unchanged. Water stress did not alter the activity and the activation state of ribulose bisphosphate carboxylase oxygenase. 相似文献
20.
Quantitative analysis of type IV collagen alpha chains in the basement membrane of human urogenital epithelium 总被引:2,自引:0,他引:2
Kiyofuji MA Iyama K Kitaoka M Sado Y Ninomiya Y Ueda S 《The Histochemical journal》2002,34(10):479-486
Type IV collagen is a major component of the basement membrane (BM), which consists of six genetically distinct (IV) chains. In this study the expression of these six (IV) chains was demonstrated immunohistochemically. In addition, the 2(IV) and 5(IV) chains were analysed quantitatively by confocal laser scanning microscopy in human urogenital epithelial BM. The 1/2(IV) and 5/6(IV) chains were immunoreactive in the epithelial BM, whereas, 3/4(IV) chains were not. The quantitative analysis revealed that the amount of 2(IV) and 5(IV) chains differed in each urogenital epithelial BM. The content of 5(IV) chains in the epithelial BM of the bladder was differentially high, and that of the foreskin was differentially low. It is concluded that the elasticity of epithelial BM of the bladder may be structurally related to the high content of 5/6(IV) chains. 相似文献