共查询到20条相似文献,搜索用时 15 毫秒
1.
Soo Hyung Eo Woo-Shin Lee Travis C. Glenn Kenneth L. Jones Eun-Jae Lee Yong-Su Park John P. Carroll Shin-Jae Rhim 《Conservation Genetics》2009,10(6):1923-1925
We isolated and characterized eight polymorphic microsatellite DNA markers from the Korean field mouse, Apodemus peninsulae. The primers developed in this study yielded an average polymorphic information content of 0.78 (range 0.44–0.90), with an
average of 10.9 alleles per locus (range 5–16). Observed and expected heterozygosities ranged from 0.46 to 1.00 and from 0.49
to 0.93, respectively. These polymorphic loci may provide useful tools for understanding the species’ genetic structure and
ecology. 相似文献
2.
Sarmast MK Salehi H Ramezani A Abolimoghadam AA Niazi A Khosh-Khui M 《Molecular biotechnology》2012,50(3):181-188
Randomly amplified polymorphic DNA (RAPD) was used as a tool to assess the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca with explants taken from orthotropic stem along with their related mother plants after treatment with
kinetin, 2iP, BA (0.02–0.26 mg/l) and TDZ (0.001–1 mg/l) to produce axillary shoots. TDZ and kinetin induced more shoot and
higher length per explant. Results showed a total of 1,676 fragments were generated with 12 RAPD primers in micropropagated
plants and their donor mother plants. The number of loci ranged from 6 in OPB 12–18 in OPY 07 with a size ranging from 250
bp in OPH 19–3500 bp in OPH 11. Cluster analysis of RAPD data using UPGMA (unweighted pair group method with arithmetic average)
revealed more than 92% genetic similarities between tissue cultured plants and their corresponding mother plant measured by
the Jaccard’s similarity coefficient. Similarity matrix and PCoA (two dimensional principal coordinate analysis) resulted
in the same affinity. Primers had shown 36% polymorphism. However, careful monitoring of tissue culture derived plants might
be needed to determine that rooted shoots are adventitious in origin. 相似文献
3.
Fifteen new microsatellite markers were isolated in rock carp, Procypris rabaudi (Tchang), from an AC-enriched genomic library. The allele number of these loci ranged from three to 13 (average 6.87 per
locus) in the tested 24 individuals. Polymorphism information content (PIC) ranged from 0.453 to 0.821 with an average of
0.719. Average observed and expected heterozygosities were 0.712 (range 0.565–0.917) and 0.772 (range 0.542–0.855), respectively.
These microsatellite markers could be useful in the population genetic study of rock carp. 相似文献
4.
Gupta S Pandey-Rai S Srivastava S Naithani SC Prasad M Kumar S 《Journal of genetics》2007,86(3):259-268
An integrated genetic linkage map of the medicinal and ornamental plant Catharanthus roseus, based on different types of molecular and morphological markers was constructed, using a F2 population of 144 plants. The map defines 14 linkage groups (LGs) and consists of 131 marker loci, including 125 molecular
DNA markers (76 RAPD, 3 RAPD combinations; 7 ISSR; 2 EST-SSR from Medicago truncatula and 37 other PCR based DNA markers), selected from a total of 472 primers or primer pairs, and six morphological markers
(stem pigmentation, leaf lamina pigmentation and shape, leaf petiole and pod size, and petal colour). The total map length
is 1131.9 cM (centiMorgans), giving an average map length and distance between two markers equal to 80.9 cM and 8.6 cM, respectively.
The morphological markers/genes were found linked with nearest molecular or morphological markers at distances varying from
0.7 to 11.4 cM. Linkage was observed between the morphological markers concerned with lamina shape and petiole size of leaf
on LG1 and leaf, stem and petiole pigmentation and pod size on LG8. This is the first genetic linkage map of C. roseus. 相似文献
5.
Cristaria plicata was an important freshwater mussel for pearl culture in China. 18 polymorphic microsatellite markers were isolated and characterized
using (CA)15-enriched genomic library of C. plicata. These loci showed high levels of genetic polymorphism testing on 60 individuals sampled from Poyang Lake of Jiangxi Province,
China. The number of alleles per locus ranged from 4 to 18. The expected (H
E) and observed heterozygosities (H
O) were 0.7232–0.8961 and 0.0000–1.0000, respectively. Four microsatellite loci were significantly deviated from Hardy–Weinberg
equilibrium and no linkage disequilibrium was found. These microsatellite loci will be useful for assessment of genetic diversity
and population structure in C. plicata. 相似文献
6.
Caitríona E. McInerney A. Louise Allcock Mark P. Johnson Paulo A. Prodöhl 《Conservation Genetics》2009,10(5):1417-1420
Eight polymorphic microsatellite loci are described for Littorina littorea (Linnaeus, 1758). Data on allelic variation in Irish and Celtic Sea samples are reported. The average number of alleles per
locus was 11 (range 4–29), and observed and expected heterozygosities ranged from 6.9 to 84.3% and from 9.4 to 95.2%, respectively.
Loci did not deviate from Hardy–Weinberg equilibrium and no linkage disequilibrium between loci pairs was detected. Microsatellites
were not highly conserved in the congeners, L. fabalis, L. saxatilis, L. compressa and L. obtusata as evidenced by a low rate of cross-amplification. These microsatellites should prove useful in population genetic studies. 相似文献
7.
Cloning and characterisation of a family of disease resistance gene analogs from wheat and barley 总被引:7,自引:0,他引:7
S. Seah K. Sivasithamparam A. Karakousis E. S. Lagudah 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):937-945
The most common class of plant disease resistance (R) genes cloned so far belong to the NBS-LRR group which contain nucleotide-binding
sites (NBS) and a leucine-rich repeat (LRR). Specific primer sequences derived from a previously isolated NBS-LRR sequence
at the Cre3 locus, which confers resistance to cereal cyst nematode (CCN) in wheat (Triticum aestivum L.) were used in isolating a family of resistance gene analogs (RGA) through a polymerase chain reaction (PCR) cloning approach.
The cloning, analysis and genetic mapping of a family of RGAs from wheat (cv ‘Chinese Spring’) and barley (Hordeum vulgare L. cvs ‘Chebec’ and ‘Harrington’) are presented. The wheat and barley RGAs contain other conserved motifs present in known
R genes from other plants and share between 55–99% amino acid sequence identity to the NBS-LRR sequence at the Cre3 locus. Phylogenetic analysis of the RGAs with other cloned R genes and RGAs from various plant species indicate that they
belong to a superfamily of NBS-containing genes. Two of the barley derived RGAs were mapped onto loci on chromosomes 2H (2),
5H (7) and 7H (1) using barley doubled haploid (DH) mapping populations. Some of these loci identified are associated with
regions carrying resistance to CCN and corn leaf aphid.
Received: 6 January 1998 / Accepted: 1 April 1998 相似文献
8.
Abolghasem Abbasi Kejani Sayed Ali Hosseini Tafreshi Sayed Mojtaba Khayyam Nekouei Mohammad Reza Mofid 《Molecular biology reports》2010,37(2):797-800
Improved and efficient methods were developed for isolating high quality DNA and RNA from different sources of Iranian Yew
(Taxus baccata L.). The methods were based on CTAB extraction buffer added with high levels of polyvinylpyrrolidone (PVP) and β-mercaptoethanol to properly remove polysaccharides and prevent oxidation of phenolics. The pellets obtained by ethanol precipitation
were washed only with Chloroform: isoamyl alcohol (24:1). So, we could successfully eliminate the dangerous phenol/chloroform
extraction steps from the isolation procedure. Both spectrophotometric (A260/A280 and A260/A230 ratios) and agarose electrophoresis analysis of isolated nucleic acids (DNA and RNA) indicated good results. DNA with the
average yield of 100–300 μg/g leaf and stem tissue and total RNA with an average yield of 20–30 μg/g cell culture and 80–100 μg/g
leaf and stem tissue of Iranian yew could be obtained. Successful amplification of pam and pds by PCR and RT-PCR, showed the integrity of isolated DNA and RNA, respectively. 相似文献
9.
Li Zhang Yonghong Zhou Chunbang Ding Ruiwu Yang Shigui Liu 《Frontiers of Biology in China》2008,3(2):170-175
We investigated the genetic variation within 32 accessions distributed to 14 species and one variety by using ISSR (inter-simple
sequence repeat) markers. The results showed that genetic variation was relatively higher among the accessions. A total of
593 bands were amplified by 12 ISSR primers, of which 535 bands (90.2%) were polymorphic. Eleven to 80 polymorphic bands were
amplified from each prime, with an average of 44.6 bands. The interspecies GS (genetic similarity) value ranged from 0.430
to 0.866, and the average was 0.620. Cluster analysis showed that all accessions could be classified into 4 groups by ISSR
markers. The different accessions in a species were clustered together, but they had genetic variation in molecular levels.
There was obvious interspecies genetic variation. Species with similar morphological characteristics and from the same areas
or neighboring geographical regions were clustered together and had close relationships. ISSR markers are useful in analyzing
interspecies variation in Kengyilia.
__________
Translated from Guihaia, 2006, 26 (4): 375–380 [译自: 广西植物] 相似文献
10.
Ashwini V. Rajwade Ritu S. Arora Narendra Y. Kadoo Abhay M. Harsulkar Prakash B. Ghorpade Vidya S. Gupta 《Molecular biotechnology》2010,45(2):161-170
The objective of this study was to analyze the genetic relationships, using PCR-based ISSR markers, among 70 Indian flax (Linum
usitatissimum L.) genotypes actively utilized in flax breeding programs. Twelve ISSR primers were used for the analysis yielding 136 loci,
of which 87 were polymorphic. The average number of amplified loci and the average number of polymorphic loci per primer were
11.3 and 7.25, respectively, while the percent loci polymorphism ranged from 11.1 to 81.8 with an average of 63.9 across all
the genotypes. The range of polymorphism information content scores was 0.03–0.49, with an average of 0.18. A dendrogram was
generated based on the similarity matrix by the Unweighted Pair Group Method with Arithmetic Mean (UPGMA), wherein the flax
genotypes were grouped in five clusters. The Jaccard’s similarity coefficient among the genotypes ranged from 0.60 to 0.97.
When the omega-3 alpha linolenic acid (ALA) contents of the individual genotypes were correlated with the clusters in the
dendrogram, the high ALA containing genotypes were grouped in two clusters. This study identified SLS 50, Ayogi, and Sheetal
to be the most diverse genotypes and suggested their use in breeding programs and for developing mapping populations. 相似文献
11.
Changwei Shao Songlin Chen Genbo Xu Xiaolin Liao Yongsheng Tian 《Conservation Genetics》2009,10(3):623-625
Chinese sea perch (Lateolabrax maculates) is one of the most important commercial species of mariculture in China. In this study, we constructed a repeat-enriched
genomic DNA library of L. maculates. Eighteen dinucleotide microsatellite markers were characterized by genotyping 32 samples. The number of alleles ranged from
three to nine, and the observed and expected heterozygosities ranged from 0.4516 to 1.0000 and from 0.4045 to 0.8676, respectively.
Significant deviations from Hardy–Weinberg expectations were detected at four loci and linkage disequilibrium between two
loci was significant after applying Bonferroni correction. The 18 highly polymorphic microsatellite markers should provide
sufficient level of genetic diversity to investigate the population structure and evaluate the breeding strategy in L. maculates. 相似文献
12.
Glyptemys insculpta is considered to be one of the most endangered freshwater turtles in North America. Here microsatellite markers were employed
to investigate the genetic variation and population structure of G. insculpta at Delaware Water Gap National Recreation Area (USA). Seven microsatellites revealed high allelic variation with 13–30 alleles
per locus. Observed and expected heterozygosities per locus ranged from 0.875–0.925 to 0.888–0.952, respectively. Pairwise
estimates of population structure (θ) ranged from 0.000–0.013 to θ estimated over all loci and aggregations was not significantly different from zero. Gene flow (Nm) was high and ranged from 19 migrants per generation to infinity in pairwise comparisons. No significant relationship between
geographic distance and genetic distance was detected. These data indicate that G. insculpta at DEWA represent a single, genetically diverse management unit for conservation. 相似文献
13.
Aliya El Nagar Matthew McHugh Toby Rapp David W. Sims Martin J. Genner 《Conservation Genetics》2010,11(3):1203-1206
Simple sequence repeat markers (SSRs, microsatellites) were characterised for skates (Elasmobranchii: Rajidae) from published
expressed sequence tags (ESTs) of Leucoraja erinacea. These were tested in four European species (Raja clavata, Raja montagui, Dipturus batis, and Leucoraja naevus). Thirteen of the fourteen amplified loci were polymorphic in at least one species. Polymorphic loci possessed on average
4.5–5.9 alleles per species, and expected heterozygosity ranged from 0.05 to 0.88. Possible null alleles were detected at
three loci, while one locus showed significant deviation from Hardy–Weinberg Equilibrium proportions. Three locus-pairs exhibited
significant linkage disequilibrium in one or more species. This marker set will be valuable for population genetic analyses
of the focal taxa, and may prove useful for studies of other skate species. 相似文献
14.
Robert Andrew King Sarah L. Harris Angela Karp Jacqueline H. A. Barker 《Tree Genetics & Genomes》2010,6(2):247-258
We present nine polymorphic di- and tri-nucleotide repeat nuclear microsatellite markers selected specifically for their use
in high throughput studies concerning the dioecious allotetraploid Salix alba–Salix fragilis willow complex. These taxa and their hybrids are difficult to discriminate using morphological characters. Thus, multiplex
reactions were developed for these microsatellite loci and their effectiveness to distinguish individuals, especially hybrids,
and their inheritance patterns in controlled crosses were determined. All loci displayed disomic–monogenic inheritance which
allowed for the genotype data to be analysed as for a diploid organism. The nine loci produced a total of 67 alleles (mean,
7.4 alleles per locus; range, 3–11 alleles) in a reference panel of 57 individuals from two germplasm collections and natural
populations. Gene diversity values (as measured by the expected heterozygosity) ranged from 0.000–0.820. A total of 53 distinct
multilocus genotypes were observed, and ordination analysis revealed three separate clusters corresponding to S. alba, S. fragilis and hybrids. The microsatellite loci described here will be used in population genetic studies to investigate genetic variation,
gene flow, levels of hybridisation and the extent of introgression in natural populations of the S. alba–S. fragilis complex. They are also useful for clonal identification, conservation and sustainable management of germplasm collections,
genetic mapping and the selection of individuals and/or certification of controlled crosses for breeding programmes. 相似文献
15.
Changwei Shao Genbo Xu Lei Wang Xiaolin Liao Yongsheng Tian Songlin Chen 《Conservation Genetics》2009,10(3):717-719
Given the ecological and commercial importance of the summer flounder (Paralichthys dentatus), there is a surprising paucity of information on the molecular genetics of this species. Some studies published to date
are concentrated on the reproduction biology. To address this shortcoming, a microsatellite-enriched genomic DNA library of
P. dentatus was generated and screened by sequencing. Twelve dinucleotide microsatellite loci were characterized by genotyping 24 samples.
The observed number of alleles ranged from three to thirteen with an average of 8.25, while the effective number of alleles
ranged from 2.21 to 8.28 with an average of 5.06. The observed and expected heterozygosities ranged from 0.0833 to 0.9583
and from 0.5594 to 0.8980, respectively. Significant deviations from Hardy–Weinberg expectations were detected at three loci
and linkage disequilibrium between two loci was significant after applying Bonferroni correction. In cross-species amplification,
three species showed at least two polymorphic loci. The 12 highly polymorphic microsatellite markers represent a powerful
molecular tool, which will allow for detailed population genetic analyses on this important marine fish. 相似文献
16.
Svetlana Milošević-Zlatanović Jelka Crnobrnja-Isailović Srđan Stamenković 《Acta theriologica》2005,50(4):429-444
The present study investigates the genetic structure of 12 roe deerCapreolus capreolus Linnaeus, 1758 population samples from Serbia, by screening a total of 334 individuals. We examined whether genetic differentiation
exists in local populations in Serbia, and addressed the question whether management policies may affect genetic structure.
The populations were analysed by multilocus protein electrophoresis, with 33 protein loci examined. Screening of 20 enzymes
and one group of general proteins revealed polymorphism at the following 12 loci: Sdh, Mdh-1, Me-1, Idh-2, 6-Pgd-1,αGpd, Ak, Pgm-1, Pgm-2, Ca, Mpi andGpi. Among samples, the proportion of polymorphic loci varied between 3–15.2% (mean 11.9%), while the average gene diversity
was in the range of 1.1–4.2%. The overall genetic differentiation was low (θ = 0.03). The comparison of two regional population
groups (northern-southern, separated by the Danube River) showed an absence of genetic differentiation between regions. Gene
flow was estimated at 8.96 migrants per generation, and was higher in the lowland than in the highland group. Three loci (Ca, 6-Pgd andGpd-1) showed clinal variation along a geographical gradient. Additional five alleles of four loci (Ak, Pgm-1, Gpi, 6-Pgd) showed significant spatial autocorrelation. Genetic distances were small (D = 0–0.004). Northern and southern populations clustered separately. For at least three populations game management practices
provide evidence for outlying genetic parameters. The observed heterogeneity in the inbreeding level was deemed more under
the influence of non-random mating strengthened by game management, than by overall selective pressure. 相似文献
17.
The Brown Eared-pheasant (Crossoptilon mantchuricum) is an endangered species endemic to China. Here we developed eight microsatellite loci using a modified biotin-capture method.
In the analyses of 28 individuals sampled, the number of alleles per locus ranged from four to nine. The expected (H
E) and observed (H
O) heterozygosities were 0.466–0.825 and 0.619–0.847, respectively. Results that eight microsatellite loci were highly polymorphic
indicated that these markers are sufficiently powerful to address such questions as genetic diversity and population genetic
structure of C. mantchuricum. 相似文献
18.
The endangered species Paeonia rockii is the most important ancestral species of the cultivated tree peonies. These well-known ornamental plants are termed the
‘King of Flowers’ in China. In this study, we investigated the genetic diversity and phylogeographic structure of 335 wild
samples from 20 populations throughout the entire distributional range of the species based on three chloroplast DNA sequences
(petB–petD, rps16–trnQ and psbA–trnH). At those loci, high levels of genetic differentiation (G
ST
= 0.94) and low levels of genetic variation (θ = 0.00185) were detected. The intraspecific phylogeny revealed four groups, the western group, the Taibai mountain group,
the northern group and the eastern group, which closely coincided with the geographic distribution of the species. A phylogeographic
structure of this kind could result from a number of integrated factors, such as allopatric fragmentation, climatic fluctuations,
increased abortion and declining germination of seeds, or lack of gene flow among populations, especially across the geographic
barrier of the high Qinling Mountains, and it could also result from adaptive evolution. For conservation purposes, each extant
population of P. rockii should be recognized as a conservation-significant unit, and a more stringent conservation strategy should incorporate in
situ and ex situ methods. 相似文献
19.
The yellow catfish Pelteobagrus fulvidraco is a freshwater fish species. Due to overfishing and pollution of freshwater ecosystems, the wild stocks of this fish reduced
substantially. We isolated and characterized 12 polymorphic microsatellites of this species. The number of alleles at the
12 microsatellite loci ranged from four to eight, with an average of 6.6/locus. The average observed heterozygosity was 0.72,
whereas the expected heterozygosity ranged from 0.60 to 0.86 (average: 0.80). All 12 microsatellites conformed to Hardy–Weinberg
Equilibrium and were in linkage equilibrium. These 12 novel microsatellites could facilitate studies of genetic diversity
and population structure of the yellow catfish to supply necessary information of conservation of the yellow catfish. 相似文献
20.
Liang Zhang Xu Liang Zhihe Zhang Fujun Shen Wenping Zhang Kun Wei Zhi Yang Rong Hou Bisong Yue Hiroshi Kamata Ken Okabayashi Shigeo Ohba Hideo Kiba Shigehisa Tsumagari Tsuneo Sato Kiichi Kanayama Injen Pan Toshi Watanabe 《Conservation Genetics》2008,9(3):787-790
Ten polymorphic microsatellite loci were characterized from two genomic DNA-enriched libraries of the red panda (Ailurus fulgens). The number of observed alleles among 35 samples of red pandas ranged from five to 12. Observed and expected heterozygosities
were 0.286–0.971 and 0.443–0.894, and the mean polymorphic information content was 0.712. All loci followed Hardy–Weinberg
expectations except Aifu-14 and Aifu-16, which may due to the presence of inbreeding or null alleles. Three pairs of loci
exhibited significant linkage disequilibrium after Bonferroni correction for multiple comparisons. These microsatellites would
be useful to strengthen population management, genetic diversity exploration, and demographic history speculation of this
species. 相似文献