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1.
NEW OBSERVATIONS ON MICROBODIES : A Cytochemical Study on CPIB-Treated Rat Liver 总被引:10,自引:7,他引:3
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The liver of male rats has been studied after CPIB stimulation by using the peroxidase reaction for localizing catalase in hepatic cells. CPIB administration leads to an increase in the number of microbodies, and it is suggested that one mechanism by which microbody proliferation occurs is a process of fragmentation or budding from preexisting microbodies. Reaction product was observed not only within the microbody matrix, but outside the limiting membrane of the microbody and in association with ribosomes of adjacent rough endoplasmic reticulum. This localization of reaction product is interpreted as evidence that catalase after synthesis on rough endoplasmic reticulum may accumulate near microbodies and may be transferred directly into these organelles without traversing the cisternae of the endoplasmic reticulum or Golgi apparatus. 相似文献
2.
PEROXIDASE ACTIVITY IN RAT LIVER MICROBODIES AFTER AMINO-TRIAZOLE INHIBITION 总被引:5,自引:4,他引:1
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The in vivo effects of 3-amino-1,2,4-triazole (AT) on the fine structure of microbodies in hepatic cells of male rats has been studied by the peroxidase-staining technique. Within 1 hr of intraperitoneal injection AT abolishes microbody peroxidase-staining, and the return of staining coincides temporally with the known pattern of return of catalase activity following AT inhibition; this is further evidence that the peroxidase staining of microbodies is due to catalase activity. Peroxidase staining reappears in the microbody matrix without evidence of either massive degradation or rapid proliferation of the organelles. Furthermore, during the period of return of activity, ribosomal staining occurs adjacent to microbodies whose matrix shows little or no peroxidase staining. These observations are interpreted as evidence that (a) catalase is capable of entering preexisting microbodies without traversing the cisternae of the rough endoplasmic reticulum or the Golgi apparatus, and that (b) the ribosomal staining is probably not cytochemical diffusion artifact and may represent a localized site of synthesis or activation of catalase. 相似文献
3.
Visualization of the microbody division inCyanidioschyzon merolae with the fluorochrome brilliant sulfoflavin 总被引:1,自引:0,他引:1
M. Miyagishima R. Itoh K. Toda H. Takahashi H. Kuroiwa T. Kuroiwa 《Protoplasma》1998,201(1-2):115-119
Summary A novel procedure is described for fluorescence staining of microbodies, which can be applied quickly and easily. We developed this technique of microbody staining with the unicellular red algaCyanidioschyzon merolae. Cyanidioschyzon merolae only contains a single chloroplast, mitochondrion, and microbody per cell, and the mitotic cycle and the organelle division cycle are easily synchronized. Knowing that the concentration of H2O2 in the microbody is higher than it is in the cytosol and other cell components, we attempted to visualize the microbody by using fluorescence microscopy to detect H2O2. Brilliant sulfoflavin (BSF), used for detecting Fe2+ in analytical chemistry, fluoresces when it reacts with Fe2+ and H2C2. We were able to specifically stain microbodies with BSF, under acidic conditions (pH 3.0 or pH 2.5) with blue-light excitation. Using this procedure, we observed division of the microbody and the effect of aphidicolin on the microbody. We also discovered that microbody division is regulated by the cell nucleus and follows division of the cell nucleus. 相似文献
4.
Summary. Division and partitioning of microbodies (peroxisomes) of the green alga Klebsormidium flaccidum, whose cells contain a single microbody, were investigated by electron microscopy. In interphase, the rod-shaped microbody
is present between the nucleus and the single chloroplast, oriented perpendicular to the pole-to-pole direction of the future
spindle. A centriole pair associates with one distal end of the microbody. In prophase, the microbody changes not only in
shape, from a rodlike to a branched form, but also in orientation, from perpendicular to parallel to the future pole-to-pole
direction. Duplicated centriole pairs are localized in close proximity to both distal ends of the microbody. In metaphase,
the elongated microbody flanks the open spindle, with both distal ends close to the centriole pair at either spindle pole.
The microbody further elongates in telophase and divides after septum formation (cytokinesis) has started. The association
between the centrioles and both distal ends of the microbody is maintained throughout mitosis, resulting in the distal ends
of the elongated microbody being fixed at the cellular poles. This configuration of the microbody may be favorable for faithful
transmission of the organelle during cell division. After cytokinesis is completed, the microbody reverts to the perpendicular
orientation by changing its shape. Microtubules radiating from the centrosomes flank the side of the microbody throughout
mitosis. The close association of centrosomes and microtubules with the microbody is discussed in respect to the partitioning
of the microbody in this alga.
Correspondence: H. Hashimoto, Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba,
Meguro-ku, Tokyo 153-8902, Japan.
Present address: M. Honda, Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo,
Kashiwa, Chiba, Japan. 相似文献
5.
Profuse appearance of microbodies was observed in the cells of methanol-utilizing yeasts in connection with the enhanced catalase activity. These microbodies were isolated successfully by means of sucrose gradient centrifugation from the methanol-grown cells of Kloeckera sp. no. 2201. Localization of a flavin-dependent alcohol oxidase as well as characteristic microbody enzymes (catalase and D-amino acid oxidase) were ascertained in the isolated microbodies, whereas formaldehyde and formate dehydrogenases were detected in the cytoplasmic region. Localization of catalase in the isolated microbody was also demonstrated by the cytochemical technique with 3,3'-diaminobenzidine. 相似文献
6.
CYTOCHEMICAL LOCALIZATION OF PEROXIDATIC ACTIVITY OF CATALASE IN RAT HEPATIC MICROBODIES (PEROXISOMES) 总被引:23,自引:8,他引:15
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H. Dariush Fahimi 《The Journal of cell biology》1969,43(2):275-288
Prominent staining of rat hepatic microbodies was obtained by incubating sections of aldehyde-fixed rat liver in a modified Graham and Karnovsky's medium for ultrastructural demonstration of peroxidase activity. The electron-opaque reaction product was deposited uniformly over the matrix of the microbodies. The microbodies were identified by their size, shape, presence of tubular nucleoids, and other morphologic characteristics, and by their relative numerical counts. The staining reaction was inhibited by the catalase inhibitor, aminotriazole, and by KCN, azide, high concentrations of H2O2, and by boiling of sections. These inhibition studies suggest that the peroxidatic activity of microbody catalase is responsible for the staining reaction. In the absence of exogenous H2O2 appreciable staining of microbodies was noted only after prolonged incubation. Addition of sodium pyruvate, which inhibits endogenous generation of H2O2 by tissue oxidases, or of crystalline catalase, which decomposes such tissue-generated H2O2, completely abolished microbody staining in the absence of H2O2. Neither diaminobenzidine nor the product of its oxidation had any affinity to bind nonenzymatically to microbody catalase and thus stain these organelles. The staining of microbodies was optimal at alkaline pH of 8.5. The biological significance of this alkaline pH in relation to the similar pH optima of several microbody oxidases is discussed. In addition to staining of microbodies, a heat-resistant peroxidase activity is seen in some of the peribiliary dense bodies. The relation of this reaction to the peroxidase activity of lipofuscin pigment granules is discussed. 相似文献
7.
MICROBODIES IN EXPERIMENTALLY ALTERED CELLS : II. The Relationship of Microbody Proliferation to Endocrine Glands 总被引:10,自引:4,他引:6
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The liver cells of intact male rats given ethyl-α-p-chlorophenoxyisobutyrate (CPIB) characteristically show a marked increase in microbodies and in catalase activity, while those of intact female rats do not. In castrated males given estradiol benzoate and CPIB the increase in catalase activity and microbody proliferation is abolished, while in castrated females given testosterone propionate and CPIB the livers show a marked increase in microbodies and in catalase activity. No sex difference in microbody and catalase response is apparent in fetal and neonatal rats. Both sexes show a sharp rise in catalase activity on the day of birth, with a rapid decline at 5 days after birth. Thyroidectomy abolishes the hypolipidemic effect of CPIB in rats, but microbody proliferation and increase in catalase activity persists in thyroidectomized male rats, indicating that microbody proliferation can be independent of hypolipidemia. Adrenalectomy does not alter appreciably the microbody-catalase response to CPIB. These experiments demonstrate that (1) in adult rats, hepatic microbody proliferation is dependent to a significant degree upon male sex hormone but is largely independent of thyroid or adrenal gland hormones; (2) hepatic microbody proliferation is independent of the hypolipidemic effect of CPIB; (3) displacement of thyroxine from serum protein may not be sufficient cause for stimulation of microbody formation. 相似文献
8.
Synopsis The effects of detergents, organic lipid solvents, and several adjuvants used in cell fractionation on the ultrastructure of the peroxisomal (microbody) membrane and its permeability to catalase have been investigated. Chopper sections of glutaraldehyde-fixed liver were incubated in the presence of various agents, followed by cytochemical staining for catalase and processed for electron microscopy. Catalase activity was also determined biochemically in the incubation medium. Marked catalase diffusion was found after treatment with 1% or 0.5% Triton X-100 or deoxycholate, as well as with 50% ethanol or acetone or 20% propanol ort-butanol. In contrast, 1% digitonin and lower concentrations of the above agents, as well as sucrose or glycerine caused selective diffusion of catalase from a limited population of peroxisomes. Tieatment with 10% polyvinylpyrrolidone (PVP), which has been used as a protective agent in the isolation of microbodies, did not produce any alteration in the fine structure and cytochemical appearance of peroxisomes. These findings concur with earlier biochemical studies on freshly isolated peroxisomes and demonstrate the susceptibility of microbodies, even in glutaraldehyde-fixed rat liver to the effects of various agents which affect the microbody membrane. A close correlation between the ultrastructural integrity of the microbody membrane and its permeability to catalase has been found. The significance of these observations for the assessment of the permeability characteristics of the microbody membrane is discussed. 相似文献
9.
Whole cell lysates of pathogenic and nonpathogenic strains of Cryptobia salmositica were subjected to subcellular fractionation using differential and isopycnic centrifugation in sucrose. The glycolytic enzymes hexokinase, fructose-1,6-biphosphate aldolase, triosephosphate isomerase, glucosephosphate isomerase and glyceraldehyde-3-phosphate-dehydrogenase and the peroxisomal enzyme catalase were associated with a microbody that had a buoyant density in sucrose of 1.21 g cm-3. Lactate dehydrogenase was detected in whole cell lysates, but not in purified organelles. A microbody with a positive reaction for catalase was detected in electron microscope sections of the pathogenic and nonpathogenic strains. These catalase-containing microbodies fused with lipid bodies and vacuoles, arose by division from pre-existing microbodies and expelled their contents into the cytoplasm of the cell. Both strains also modified the catalase content in their microbodies. Under aerobic conditions, they metabolized glucose to pyruvate and lactate. We conclude that part of the glycolytic pathway in C. salmositica is compartmentalized in a microbody called the glycosome. 相似文献
10.
Ultrastructure and isolation of glyoxysomes (microbodies) in zoospores of the fungusentophlyctis sp. 总被引:1,自引:0,他引:1
Martha J. Powell 《Protoplasma》1976,89(1-2):1-27
Summary A correlative approach, involving light and electron microscopic, cytochemical, and biochemical techniques, was used to study the structure and function of microbodies in zoospores ofEntophlyctis sp. The same population of microbodies already existing in the zoosporangium appeared to be segregated into zoospore initials during cytoplasmic cleavage. Microbodies laid at the anterior end of zoospores and were part of an organized assemblage of organelles, the microbody-lipid globule complex. In the microbody-lipid globule complex, endoplasmic reticulum occurred on the surface of the lipid globules toward the zoospore's exterior, and the microbody, subtended by mitochondria, was appressed to the opposite surface of the lipid globule. The organization of the microbody-lipid globule complex changed as the zoospore swam and encysted. As lipid globules coalesced, the microbody-lipid globule complex became disorganized. After lipid globule coalescence was completed, the microbody-lipid globule complex regained its order, and several microbodies were clustered adjacent to a single lipid globule. The microbodies persisted even in the encysted zoospore, but they were found on all sides of the lipid globule.Microbodies isolated from zoospores contained catalase as well as malate synthase and isocitrate lyase, two enzymes of the glyoxylate cycle. When zoospores encysted greater activities of these glyoxylate cycle enzymes could be detected. The presence of glyoxylate cycle enzymes and the close association between the microbody and lipid globule suggest that microbodies function as glyoxysomes in zoospores and encysted zoospores. The functional significance of the morphological organization of the microbody-lipid complex is discussed in terms of energy production and the conversion of storage lipid into structural components of the cell. 相似文献
11.
Effects of catalase inhibitors on the ultrastructure and peroxidase activity of proliferating microbodies 总被引:1,自引:0,他引:1
Summary The relationship between the formation of microbodies and catalase synthesis in the hepatic cells of male rats was examined with conventional electron microscopy and with the peroxidase staining technic for demonstrating catalase. Daily intraperitoneal injections of ethyl--p-chlorophenoxyisobutyrate (CPIB) for 5 days caused a profound increase in microbody numbers without markedly affecting the appearance of the matrix material and all microbodies retained peroxidase activity. A single injection 5 days before sacrifice of 3-amino-1,2,4-triazole (AT), an inhibitor of catalase activity but not catalase synthesis, did not affect their numbers, appearance of matrix material or peroxidase staining. Twice daily injection for 5 days of allylisopropylacetamide (AIA), an inhibitor of catalase synthesis, also did not affect microbody numbers but lowered the electron-density of the microbody matrix and abolished peroxidase staining. After combined administration of these drugs, the number of hepatic microbodies increased but they did not contain peroxidase activity. The results suggest strongly that microbody proliferation is dependent not on catalase synthesis but on synthesis of non-enzymatic protein.This study was supported by research grant HD-01337 from the Institute of Child Health and Human Development, United States Public Health Service. The authors thank Mrs. Judith Henrickson, and Mr. Gerald Haiden for technical assistance. Dr. Legg is at present on leave from the Department of Anatomy, Monash University, Melbourne, Australia. 相似文献
12.
Shin-ya Miyagishima Ryuuichi Itoh Kyoko Toda Hidenori Takahashi Haruko Kuroiwa Tsuneyoshi Kuroiwa 《Planta》1998,206(4):551-560
The formation of the plastid-dividing ring (PD ring) and mitochondrion-dividing ring (MD ring) was studied in a highly synchronous
culture of the unicellular red alga Cyanidioschyzon merolae. The timing and the order of formation of the MD and PD rings were determined by observing organelles around the onset of
their division, using transmission electron microscopy. In C. merolae, there is one chloroplast and one mitochondrion per cell, and the shape of the chloroplast changes sequentially from acorn-like,
to round, to trapezoidal, to peanut-shaped, in that order, during the early stage of chloroplast division. None of the cells
with acorn-shaped or round chloroplasts contained organelles with PD rings or MD rings, while all of the cells with peanut-shaped
chloroplasts contained organelles with both PD rings and MD rings. In cells with peanut-shaped chloroplasts, the PD and MD
rings were double ring structures, with an outer ring located on the cytoplasmic face of the outer membrane of the organelle,
and an inner ring located in the matrix beneath the inner membrane. These results suggested that the double ring structures
of the PD ring and the MD ring form when chloroplasts are trapezoidal in shape. Detailed three-dimensional observation of
cells with trapezoidal chloroplasts revealed the following steps in the formation of the double ring structures of the PD
and MD rings: (i) the inner ring of the PD ring forms first, followed by the outer ring; (ii) then the MD ring forms and becomes
visible; (iii) when the double ring structures of the two rings have formed, the microbody then moves from its remote location
to the plane of division of the mitochondrion and contraction of the PD and MD rings commences. These steps were also confirmed
by computer-aided three-dimensional reconstruction of the images from serial thin sections. This study reveals the order of
formation of the double ring structures of the PD and MD rings, and the behavior of the microbody around the onset of division
of plastids and mitochondria. The results also provide the first evidence that the inner PD ring is not a tension element
formed by the contractile pressure but a definite structure, independent of the outer ring.
Received: 31 March 1998 / Accepted: 14 May 1998 相似文献
13.
Martha J. Powell 《Protoplasma》1979,98(3):177-198
Summary The ultrastructure of microbodies in developing zoosporangia ofEntophlyctis variabilis was studied by three dimensional reconstructions from serial sections and by cytochemical localization of catalase activity. The morphology of microbodies and the spatial association of microbodies with other organelles varied during fungal development. In incipient zoo-sporangia, granular dilations resembling microbodies arose from rough ER. Young, enlarging zoosporangia contained elongate, contorted microbodies continuous with ER and aligned along bundles of microtubules. Oval, paired microbodies, lying on each side of an ER cisternae, were found in all zoosporangia, but in older zoosporangia this configuration of microbodies predominated. Analysis of serial sections revealed that these oval, paired microbodies were sometimes continuous with each other, with ER, and also apparently with the ER cisterna interposed between them. Other paired, oval microbodies were clearly discrete. Constrictions were found along the length of elongate microbodies and at junctions between oval microbodies. These constrictions may represent stages in fragmentation of microbodies from pre-existing microbodies. These observations suggest that microbodies originated in three ways: 1. as local dilations in tubular ER, 2. as lateral buds from opposite sides of ER cisternae, and 3. as fragments from elongate microbodies.Microbodies were consistently spatially associated with ER, nuclear envelopes, and mitochondria. The cisterna of ER passing between paired microbodies sometimes extended into a branching, tubular system of ER which curved around the side of one microbody and lay between this microbody and the forming face of a dictyosome. The cytochemical localization of thiamine pyrophosphatase activity in this cisterna when it is not associated with dictyosomes suggests a role in metabolic control. These spatial associations indicate that the microbody assemblage with other organelles represents functional units where propinquity to other organelles and intraluminal continuities insure a system for transport of substrates and products. 相似文献
14.
The structure and life history of insect microbodies are described during the development of the fat body from the 4th to 5th larval molt through the 5th to pupal molt. The mature microbodies are flattened spheres about 1.1 x 0.9 µ, with a depression on one side where a dense mass connects the limiting membrane to the core of coiled tubules. They contain catalase and urate oxidase. The precise synchrony of development of insect cells during the molt/intermolt cycle makes it easy to study the life history of particular organelles. Phases of growth are correlated with the hormonal milieu. Mature 4th stage microbodies decrease in size before ecdysis to the 5th stage when they atrophy at the same time as the new 5th stage generation arises. The 5th stage microbodies form as diverticula of the RER and, grow while confronted by RER cisternae. The mature microbodies decrease in size when the fat body engages in massive larval syntheses. At the end of the 5th larval stage, the microbodies are invested by isolation membranes and destroyed before pupation. There are thus two mechanisms for microbody destruction: atrophy of the 4th stage organelles and isolation with autophagy at the end of the 5th stage. 相似文献
15.
Susumu Kawamoto Atsuo Tanaka Midori Yamamura Yutaka Teranishi Saburo Fukui 《Archives of microbiology》1977,112(1):1-8
Microbodies appearing abundantly in n-alkane-grown cells of Candida tropicalis pK 233 were isolated by means of sucrose density gradient centrifugation. Electron microscopical observation showed that the microbodies isolated were intact. Localization of catalase and d-amino acid oxidase in the isolated microbodies was confirmed. Isocitrate lyase, malate synthase and NADP-linked isocitrate dehydrogenase were also located in the microbody, but malate dehydrogenase, citrate synthase, aconitase and NAD-linked isocitrate dehydrogenase were not. Neither cytochrome P-450 nor NADPH-cytochrome c reductase, the components involved in the n-alkane hydroxylation system of the yeast, were detected in the microbody fraction. 相似文献
16.
Susana Valenciano J. Ramón De Lucas A. Pedregosa Inmaculada F. Monistrol F. Laborda 《Archives of microbiology》1996,166(5):336-341
Aspergillus nidulans is able to grow on oleic acid as sole carbon source. Characterization of the oleate-induced β-oxidation pathway showed the
presence of the two enzyme activities involved in the first step of this catabolic system: acyl-CoA oxidase and acyl-CoA dehydrogenase.
After isopicnic centrifugation in a linear sucrose gradient, microbodies (peroxisomes) housing the β-oxidation enzymes, isocitrate
lyase and catalase were clearly resolved from the mitochondrial fraction, which contained fumarase. Growth on oleic acid was
associated with the development of many microbodies that were scattered throughout the cytoplasm of the cells. These microbodies
(peroxisomes) were round to elongated, made up 6% of the cytoplasmic volume, and were characterized by the presence of catalase.
The β-oxidation pathway was also induced in acetate-grown cells, although at lower levels; these cells lacked acyl-CoA oxidase
activity. Nevertheless, growth on acetate did not cause a massive proliferation of microbodies in A. nidulans.
Received: 8 March 1996 / Accepted: 5 August 1996 相似文献
17.
Shin-ya Miyagishima Ryuuichi Itoh Kyoko Toda Haruko Kuroiwa Tsuneyoshi Kuroiwa 《Planta》1999,207(3):343-353
The time courses of chloroplast and mitochondrial division and the morphological changes in the plastid-dividing ring (PD
ring) and mitochondrion-dividing ring (MD ring) during chloroplast and mitochondrial division were studied in Cyanidioschyzon merolae De Luca, Taddei and Varano. To accomplish this, chloroplast and cell division of living cells were continuously video-recorded
under light microscopy, and the morphological changes in the PD and MD rings were analyzed quantitatively and three-dimensionally
by transmission electron microscopy (TEM). Under the light microscope, the diameters of the chloroplast and the cell decreased
at uniform velocities, the speed depending on the temperature. To study in detail the sequential morphological change of the
mitochondrion in M phase and the contractile mechanism in the divisional planes of the chloroplast and the mitochondrion,
we observed the PD and MD rings, which are believed to promote contraction, under TEM, using the diameter of the chloroplast
as an index of the time. Three PD rings (an outer PD ring on the cytoplasmic face of the outer envelope, a middle PD ring
in the intermembrane space, and an inner PD ring on the stromal face of the inner envelope) were clearly observed, but only
the outer MD ring could be observed. The PD ring started to contract soon after it formed, while the contraction of the MD
ring did not occur immediately after formation, but was delayed until the contraction of the PD ring was almost complete.
Once the MD ring began to contract, the rate of decrease of its circumference was 4 times as high as that of the PD ring.
As the outer PD and MD rings contracted, they grew thicker and maintained a constant volume, while the thickness of the inner
PD ring did not change and its volume decreased at a constant rate with contraction. In the early stage of contraction, the
widths of the three PD rings increased in order, from the outer to the inner ring. With contraction, their widths changed
at different rates until they came to have much the same width. In cross-section, the MD ring was wider where it was next
to the chloroplast than at the opposite side, adjacent to the nucleus in the early stage of contraction. By the late stage,
the widths of the two sides became equal. In our observations, the microbody elongated along the outer MD ring and touched
the outer PD ring during contraction of the PD and MD rings. These results clearly revealed differences between the mode of
contraction of the outer, middle, and inner PD rings, and between the PD and the MD rings. They also revealed the coordinated
widening of the three PD rings, and suggested that the microbody plays a role in the contraction of the PD and MD rings.
Received: 1 July 1998 / Accepted: 1 September 1998 相似文献
18.
Luigi Brighigna Ambretta Cecchi Fiordi Maria R. Palandri 《American journal of botany》1982,69(7):1179-1184
Organelles morphologically similar to microbodies have been found in several tissues of atmospheric species of Tillandsia from different habitats. The presence of catalase was demonstrated by the DAB reaction thus confirming the microbody nature of these organelles. They are a feature of the Tillandsia species with normal photosynthetic carbon fixation and with CAM. Their size is consistently small. The nucleoid observed in the microbodies shows a characteristic morphology which has not been reported before within other plant microbodies. This nucleoid is composed of minute tubular structures, for which the authors here propose a three-dimensional arrangement. 相似文献
19.
Bernt Gerhardt 《Planta》1973,110(1):15-28
Summary The enzyme patterns in sunflower cotyledons indicate that the glyoxysomal function of microbodies is replaced by the peroxisomal function of these organelles during the transition from fat degradation to photosynthesis. The separation of the microbody population into glyoxysomes and peroxisomes during this transition period is reported. The mean difference in density between the activity peaks of glyoxysomal and peroxisomal marker enzymes on a sucrose gradient was calculated to be 0.007±0.004 g/cm3 and turned out to be significant (t=7.8>4.04=t
5;0.01). The activity peak of catalase coincides with that of isocitrate lyase in early stages of development, but shifts to the activity peak of peroxisomal marker enzymes during the transition period. No isozymes of the catalase could be detected by gel electrophoresis in the microbodies with the two different functions.During the rise of the peroxisomal marker enzymes no synthesis of the common microbody marker, catalase, could be demonstrated using the inhibitor allylisopropylacetamide. Using D2) for density labeling of newly-formed catalase, no difference is observed between the density of catalase from cotyledons grown on 99.8% D2O during the transition period and the density of enzyme from cotyledons grown on H2O. The activity of particulate glycolate oxidase is reduced 30–50% by allylisopropylacetamide, but is not affected by D2O. The chlorophyll formation in the cotyledons is strongly inhibited by both substances. 相似文献
20.
Rajamani Karthikeyan T. Manivasagam P. Anantharaman T. Balasubramanian S. T. Somasundaram 《Journal of applied phycology》2011,23(2):257-263
The present study was designed to investigate the prophylactic effect of extracts of the brown alga Padina boergesenii against potent nephrotoxic agent ferric nitrilotriacetate (Fe-NTA), in blood circulation of rats. Administration of Fe-NTA
for seven consecutive days significantly enhanced lipid peroxidation accompanied with reduction in glutathione content. Together
with this, the level of antioxidant enzymes, glutathione peroxidase, superoxide dismutase, and catalase was significantly
(P < 0.05) diminished. Pretreatment of rats with P. boergesenii (150 mg kg−1 body weight) reversed Fe-NTA-induced oxidative damage in lipid peroxidation and glutathione content significantly (P < 0.05). Further, the activity of antioxidant enzymes was also restored significantly. In order to assess the role of polyphenolic
components in the relevant activity, phenolic contents of the extract was found to be 1.78 ± 0.02% in the methanol extract
and 1.30 ± 0.30% in the diethyl ether extract. Hence, the present results confirm that the brown alga P. boergesenii preclude its role in Fe-NTA-induced oxidative damage and hyperproliferative response in circulation. 相似文献