Transport of Abscisic Acid-2-C-14 in Intact Pea Seedlings

When abscisic acid-2-C-14 (AbA-2-C-14), 1 μg in 5 μl 40% ethanol, is applied to the apical bud of light-grown pea seedlings, C-14 is translocated downwards only in very small amounts and does not enter the root. In contrast to this, C-14 from indoleacetic acid-C-14 (IAA-2-C-14) applied in the same manner is translocated to the root where it accumulates. When AbA-2-C-14 is injected to the stem tissue at the apical bud, more labelled material is transported downwards than after application to the surface. Application of AbA-2-C-14 to an expanded leaf results in considerable accumulation of C-14 in the growing apical parts and in the lateral roots.     

Distribution of C-14 from glucose-1-C-14 in the lipid fractions of Debaryomyces hansenii

Merdinger, Emanuel (Roosevelt University, Chicago, Ill.), and Rosalind H. Frye. Distribution of C(14) from glucose-1-C(14) in the lipid fractions of Debaryomyces hansenii. J. Bacteriol. 91:1831-1833. 1966.-Debaryomyces hansenii cells were grown in a medium containing yeast extract, malt extract, glucose, sodium chloride, and nutrient salts, to which glucose-1-C(14) was added. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total C(14) added to the culture medium, the neutral lipid fractions contained 21.06% while the phospholipid portions contained only 0.89%. The highest amount of C(14) among the neutral lipids was found in the fraction containing the hydrocarbons (11.64%). Among the phospholipids, the highest amount (0.66%) was found in phosphatidyl serine and phosphatidyl ethanolamine.     

Preparation of 2-C- and 3-C-cyano-2-enopyranoside derivatives and their epoxidation

Methyl 4,6-O-benzylidene-2-C- and 3-C-cyano-2,3-dideoxy-D-erythro-hex-2-enopyranosides and -2-enitols were prepared and their epoxidation was performed.