三倍体欧州山杨的试管繁殖

InvitroPropagationofPopulustremulaCAOChuan－Jian(NingxiaResearchCentreofForestryProtection,Yinchuan750004)RENYu-Fen,HUANGHui(NingxiaForestryResearcgInstitute,Yinchuan750004)1植物名称三倍体欧州山杨（Populus．tremula）。2材料类别由德国引进的无病毒三倍体植株顶芽、腋芽。3培养条件不定芽诱导培养基为：（1）MS＋BA0．5mg·L－1（单位下同）;（2）MS＋BA1＋IAA0．2;（3）MS＋BA1．5＋IAA0．2;（4）MS＋BA2＋IAA0．2。生根培养基为：（5）MS＋IBA0．1＋NAA0．1;（6）1／2MS＋IBA0．1＋NA…     

大花飞燕草的组织培养及植株再生

1植物名称大花飞燕草（Delphiniumgrandiflorum）,商品名“美丽飞燕草太平洋混色”。2材料类别无菌种子苗真叶。3培养条件基本培养基为MS。（1）种子萌发培养基：1／2MS＋6－BA0．sing·L（单位下同）＋NAA0．2;（2）愈伤组织诱导培养基：MS十6－BAZ5十NAA0．5十IAA0．2;（S）芽分化培养基：MS＋6－BA3＋NAA0．3;（4）生根培养基：MS＋IBA0．5。培养基（1）、（2）中添加3％蔗糖、0．3％琼脂,培养基（3）、（4）中添加3％蔗糖、0．8％琼脂,PHS．8。培养温度25士loC,光照14h·d‘,光照度1500lX。4生长与分化情况4．1…     

油桐叶片愈伤组织诱导及植株再生

以油桐无菌苗叶片为试材,研究不同种类及浓度的植物生长调节剂对愈伤组织诱导、分化、增殖及生根的影响。结果表明：叶片愈伤组织的最佳诱导培养基为1／2MS＋2．omg·L-16-BA＋1．0mg·L～2,4-D,诱导率达100％;最佳分化培养基为1／2MS＋3．0mg·L～6-BA＋0．1mg·L-1。IBA＋0．05mg·L—IAA,分化率为86．36％;最佳继代增殖培养基MS＋3．0mg·L～6-BA＋0．05mg·L。IBA;最佳生根培养基为1／2MS＋O．1mg·L-1。IBA,生根率93．83％。炼苗后移栽到泥炭土：珍珠岩：蛭石=2：1：1的基质中,成活率达92％以上。     

草莓的组织培养与快速繁殖研究

通过对草莓的茎尖生长点进行组织培养,获得草莓脱毒苗,并以此为试材,对草莓的快速繁殖以及生根培养进行了研究。结果表明：芽的最适诱导分化培养基为MS BA0．5mg／L IAA0．05mg/L,诱导率可达70％;最适增殖培养基为MS BA0．2～1．0mg／L,增殖倍率可达3．2;试管苗在1／2MS附加IAA0．5me／L的培养基上,经过10～15d的培养,95％生根成苗。     

日本花椒的组织培养

1植物名称日本花椒（Zanthoxylumjaponica）。2材料类别嫩茎段。3培养条件培养基：（1）分化培养基：MS＋BA0．5mg／L（单位下同）＋IBA0．1;（2）成苗培养基：Ms＋BA0．2＋GAo5十三BAof;（3）生根培养基：回／2MS＋IAA0．2＋IBA0．l。培养温度24～26C,光照度2000～2500lx,每日光照14h。毛生长与分化情况（）将消毒后的带l～2胶芽的嫩茎段接种到分化培养基上,约1个月能长出5～8个3cm左右高的丛生芽。把小芽单个切开接种到成苗培养基上,生长三周后长到5cm高以后,从上部3cm处切下供生根,将下部带芽的茎段接种到新的分化培…     

大花茉莉的组织培养和植株再生

王植物名称大花茉莉（Jasminu。peand～m）。2材料类别茎节、叶片。3培养条件3．五诱导培养基（l）MS＋6－BA0．2～50mg／L（单位下同）;（2）MS＋6－BA0．2～5．0＋KT0．2～2．0＋IAA0．l～1．0;（3）SH＋6－BA0．2～5．0＋NAA0．l～1．0;（4）改良White＋KT02～2．0＋NAAof～1．co3．2生根培养基（回）l／2MS＋IBA0．l～1．0;（2）1／2MS＋IAA0．回～回．0;（3）1／2MS＋NAA0．1～1．0＋2,4－D0．0］十活性炭0．l％。以上均采用固体培养,琼脂06％,蔗糖2％,PH58。诱导培养基中附加少量的水解酪蛋白0．2％～05…     

尾巨桉胚培养与快速繁殖研究(简报)

尾巨桉种胚在改良H＋2,4-D0．5mg／L＋6-BA0．1mg／L＋蔗糖3％培养基上诱导形成愈伤组织,每个愈伤组织块在改良H＋6-BA1．0mg／L＋NAA0．5mg／L＋蔗糖5％培养基上分化形成芽点,经30d继代培养,产生有效苗30～50株。利用改良MS＋6-BA0．4mg／L＋NAA0．2mg／L＋蔗糖3％培养基进行壮苗,改良MS＋ABT0．6mg／L＋IBA0．2mg,L＋蔗糖1．5％培养基进行生根诱导．有效成苗率达95％以上。移栽成活率达98％．     

枣叶片离体培养再生植株

PlantletRegenerationfromLeavesCulturesofZizyphusiuiubaCHENZong－Li,YANZhi－Lian,QILong（Denyt17mllofmp,You’onl／nll＊,ndy,Yan’as716000）1植物名称枣（凯W…。Wwi）。2材料类别俗名“狗头枣”的无菌试管苗的叶片。3培养条件（l）叶片愈伤组织诱导及继代培养基：MS＋6－BA0．3mg／L（单位下同）＋2,4D20;（2）芽分化培养基：MS＋6－BAI．0＋IBA0．2＋D一泛酸钙1．0十活性发0．5％;（3）芽生长培养基：1／2MS＋6－BA0．2＋IAA0．04＋D一泛酸钙1．0;（4）芽增殖培养基：1／2MS＋6－BA0．4＋IAA0．0…     

新铁炮百合的离体快速繁殖

1植物名称新铁炮百合（Liliumlongiflorum×L．formosanum,铁炮百合和高砂百合的杂交种）。2材料类别鳞片。3培养条件以MS为基本培养基。（1）鳞片初次接种培养基：1／3MS＋6－BA1mg·L-1（单位下同）＋IBA0．02＋GellanGum（美国生产的微生物多糖培养基固化剂）2g·L-1;（2）增殖培养基：MS＋6－BA1．0～1．5＋IBA0．2;（3）球茎形成培养基：MS＋6－BA0．2～0．5＋IAA0．2＋NAA0．05;（4）生根培养基：1／2MS＋IBx0．5。培养基含蔗糖25g·L－1,除（1）外,其它培养基附加普通琼脂5．5g·L－1,pH5．8。培养温度22～25…     

杉叶藻的组织培养与快速繁殖

1植物名称杉叶藻（Hippuris vulgari L.）。 2材料类别当年生茎尖或茎段。3培养条件基本培养基为加入0.05%内生菌型抑菌剂“山农一号”的MS培养基。（1）初代培养基：1／2MS＋TDZ1．0mg·L^-1（单位下同）＋IBA0-0．5;（2）增殖培养基：MS＋6－BA3．0＋IBA0．5＋KT0．05＋CCC0．05;（3）生根培养基：1／2MS＋IBA2．0。     

乔纳金叶片培养胚状体发生体系的建立

利用乔纳金无菌苗叶片培养,成功地诱导出胚状体并获得再生植株。具体步骤如下:Ⅰ.在MS BA2.0mg.L-1 IAA6.0mg.L-1 2,4-D0.3mg.L-1培养基上预诱导6d;Ⅱ.在MS BA2.0mg.L-1培养基上胚性细胞发生胚状体;Ⅲ.在MS BA0.5mg.L-1 IAA0.1mg.L-1上壮苗培养20d;Ⅳ.在MS IBA0.8mg.L-1 IAA0.7mg.L-1培养基上小植株生根。     

罗汉果组培繁殖的技术要点

报道罗汉果组培繁殖的各项主要技术要点,包括组培条件、培养基的配制、外植体的选取与消毒、接种与培养、种源保存、炼苗与移栽、苗木包装与运输等。提出了5种培养基参考配方,即茎段诱导培养:MS+BA0.5～1.0mg/L+IAA(NAA)0.05～0.1mg/L+白糖3%+琼脂4.5g/L,pH5.8;茎尖诱导培养:MS+BA0.5～1.0mg/L+NAA0.05～0.1mg/L+椰子水100mL+白糖3%+琼脂4.5mg/L,pH5.8;继代培养(丛生芽方式):MS+BA0.3～0.7mg/L+NAA0.05/IAA0.1mg/L+白糖3%+琼脂4.5mg/L,pH5.8;继代培养(微型扦插方式):MS+BA0.1mg/L+IAA0.3mg/L+活性炭0.07g/L+白糖3%+琼脂4.5mg/L,pH5.8;生根培养:MS+BA0.07mg/L+IBA0.15mg/L+IAA0.1mg/L+活性炭0.1g/L+白糖3%+琼脂4.5mg/L,pH5.8。分析了外植体培养过程中可能出现的不良状况的原因并提出预防措施,明确了炼苗移栽的适宜条件并制定出相应的管理方法。形成了一套较为完整的罗汉果组培苗繁殖生产技术规程。     

哑特猕猴桃微繁工艺流程的研究

选用哑特猕猴桃腋芽为外植体。在不同发育阶段适宜培养基组成：芽增殖生长培养基为ＭＳ＋６－ＢＡ０．５ｍｇ／Ｌ＋ＩＡＡ０．１ｍｇ／Ｌ（或者ＩＢＡ０．１ｍｇ／Ｌ;ＮＡＡ０．１ｍｇ／Ｌ）,其在苗基部呈辐射状萌发多个嫩枝与主茎伸长生长同步进行的繁殖特点,有利提高繁殖率。４０ｄ繁殖系数为５－７。诱导生根培养基为ＭＳ＋ＩＢＡ０．５ｍｇ／Ｌ＋ＩＡＡ０．２ｍｇ／Ｌ,生根率达９７．１％,培养３０ｄ,平均苗高３．５ｃｍ,     

马铃薯离体再生及人工种子研究

马铃薯３个品种虎头,克４和Ｆａｖｏｒｉｔａ的茎叶外植体在ＭＳ＋１ｍｇ／ＬＮＡＡ＋１ｍｇ／ＬＢＡ培养基上形成愈伤组织。在ＭＳ＋０２ｍｇ／ＬＮＡＡ＋１ｍｇ／ＬＢＡ培养基上,愈伤组织分化产生不定芽。在ＭＳ＋００５ｍｇ／ＬＮＡＡ培养基上,不定芽生根形成再生完整植株。０２～０３ｃｍ大小的不定芽反复继代可持续增殖。有３个以上叶片的不定芽在ＭＳ＋５ｍｇ／ＬＢＡ＋００５ｍｇ／ＬＩＢＡ培养基上和黑暗条件下,在侧芽或顶芽部位形成微型薯。用４％海藻酸钠和２％氯化钙溶液包裹０２～０３ｃｍ大小的不定芽或直径为０２～０３ｃｍ的微型薯制成微芽人工种子和微薯人工种子。在４℃下贮存２个月后,微芽人工种子和微薯人工种子在有菌腐殖土壤中播种２１ｄ的萌发率分别是１５７％和９６２％。     

甘蓝下胚轴原生质体再生植株

经纯化后,甘蓝下胚轴原生质体的产量为1．5×10⁶g^-1(Fw),采用液体浅层培养的方法进行培养。2～3d后,发生第一次分裂,第10天,统计分裂频率为6l％,5周内形成大量的细胞团和小愈伤组织,统计植板率为1．1％,把小愈伤组织转到与原生质体培养基相同激素的MS固体培养基上增殖。当愈伤组织长到3～5mm大小时,接到分化培养基上,芽分化率为46.7％．分化出来的芽长到3～4cm长时,从基部切下,插入生根培养基,两星期左右即可长成完整植株。     

大果良种沙棘愈伤组织诱导及植株再生的研究

大果良种沙棘的幼嫩茎尖,茎段外植体接种在MS,1／2MS附加不同浓度配比的IAA,IBA,BA,NAA培养基上可诱导茎尖及腋芽生长,将诱导产生的无性系芽接种在MS或1／2MS附加BA0．3－0．5mg/L,NAA0．05mg/L的培养基上可形成丛生芽,同时在小叶片和嫩茎上诱导产生愈伤组织,继续培养愈伤组织表面形成大量的绿色突起,进一步分化成不定芽,在相同培养基上,不定芽上可直接产生不定芽,从而形成多达数百个的不定芽族,不定芽长至3cm时切下转至1／2MS附加IAA或IBA 0．2mg/L的培养基上可生根而形成完整 的再生植株。     

In vitro clonal propagation of Nyctanthes arbor-tristis

Rapid shoot multiplication of Nyctanthes arbor-tristis L. was achieved from axillary meristems on Murashige and Skoog (MS) basal medium supplemented with 1.0–1.5 mg dm⁻³ 6-benzylaminopurine (BA), 50 mg dm⁻³ adenine sulfate (Ads) and 3 % (m/v) sucrose. Inclusion of indole-3-acetic acid (IAA) in the culture medium along with BA + Ads promoted a higher rate of shoot multiplication. Maximum mean number of microshoots per explant (6.65) was achieved on the MS medium supplemented with 1.5 mg dm⁻³ BA, 50 mg dm⁻³ Ads and 0.1 mg dm⁻³ IAA after 4 weeks of culture. The elongated shoots rooted within 13 to 14 d on half-strength MS medium supplemented with either indole-3-butyric acid (IBA), IAA or 1-naphthaleneacetic acid (NAA) with 2 % sucrose. Maximum percentage of rooting was obtained on medium having 0.25 mg dm⁻³ IBA and 0.1 mg dm⁻³ IAA. About 70 % of the rooted plantlets survived in the greenhouse. The in vitro raised plants were grown normally in the field.     

毛刺槐花药培养及再生植株的获得

以毛刺槐的花药为材料,开展其组织培养和植株再生系统的研究。结果显示：将毛刺槐的花药接种在MS附加2,4—D0．1mg／L和BA3．0mg／L的培养基上,20d时花药愈伤组织诱导率可达41．5％。花药愈伤组织在MS附加BA5．0mg／L的分化培养基上继代培养2个月后,可分化出许多绿色的芽点,待不定芽长至2—3cm高时将其切下,转入MS附加IBA1．0mg／L的生根培养基上,2周后即可得到完整的再生植株。同时,研究就4℃低温预处理和蔗糖浓度对毛刺槐花药培养的影响进行了研究和讨论。     

Plant regeneration from axillary buds of a mature tree of Ficus religiosa

Dormant axillary buds of nodal explants collected from a mature (35-year-old) tree of Ficus religiosa L. sprouted on MS medium supplemented with 6-benzyladenine (BA, 5 mg l^–1) and indole-3-butyric acid (IBA, 0.2 mg l^–1 ) within 4 days. Multiple shoots were obtained when these explants were transferred to MS medium containing 1.5 mg l^–1 BA and 1.5 mg l^–1 adenine sulphate (ADS). These multiple shoots (1 cm) were allowed to elongate to a height of 4–5 cm by maintaining them on MS medium containing 2 mg l^–1 BA, 0.5 mg l^–1 1-naphthalene acetic acid (NAA), and activated charcoal (0.3% w/v). Nodal segments taken from these in vitro-proliferated shoots yielded multiple shoots when cultured on the multiple shoot-inducing MS medium mentioned above. Root induction in these shoots (4–5 cm in height) was achieved by transferring them onto MS medium supplemented with 2 mg l^–1 IBA and 0.1 mg l^–1 NAA for 1 week; upon transfer to half-strength MS basal medium these shoots exhibited root proliferation. These rooted plantlets were successfully established in soil after a short period of acclimatization. Received: 17 April 1997 / Revision received: 2 September 1997 / Accepted: 20 September 1997     

Study on Tissue and Protoplast Culture of Wild Cotton (Gossypium davidsonii)

This paper deals with the study on the condition of callus formation, embryogenesis, organogenesis, plant regeneration and protoplast culture of wild cotton (G. davidsonii) Callus cultures derived from several organs such as root, stem, leaf, cotyledon and hypocotyl. The results obtained in these cultures showed that the modified MS medium containing 2,4-D 1.0+KT 0.1; 2,4-D 0.1+KT 0.01; NAA (IAA) 2.0+KT 0.1 and NAA (IAA) 1.0+KT 0.1 mg/L were favorable to callus formation. Modified MS medium containing 2,4-D was suitable for initiated callus of G. davidsonii Besides, suspension cultures from callus of G. davidsonii were saccessfully initiated. Optimum concentration of 6BA (or ZT, or 2ip) and NAA (IAA) was for shooting, somatic embryo or leaf formation. Plantlets regenerated from somatic embryo at lower concentration of 6BA, or ZT, or 2ip. As to protoplast culture of this species, the age and physiological condition of callus or suspension cells and concentration of enzymes used for protoplast isolation affected the yield and survival of protoplasts. Protoplast of this species cultured in modified MS medium containing 2,4-D 0.5+NAA 0.5+ZT 0.1–0.2 mg/L. and divied after 3–4 days. The rate of division was 3--4% and cell cluster formed after 14 days, then these cells died.