Selection of insect parasitic nematodes for biological control of the garden chafer,Phyllopertha horticola

Larvae ofPhyllopertha horticola L. (Coleoptera: Scarabaeidae) cause increasing problems on sports fields and lawns in NW-Europe. A biological control programme using insect parasitic nematodes is being developed. This paper contains the results of bioassays with various species and isolates of the nematode generaHeterorhabditis andSteinernema. In bioassays in small pots with moist sand, most of the nematode isolates gave 30–60% mortality against each of the three larval stages. The susceptibility of the grubs for nematode infection generally increased with larval development.H. bacteriophora, H. heliothidis, H. megidis, a DutchHeterorhabditis isolate NLH-E87.3 andS. glaseri 326 showed the highest mortality rates, with nearly 100% mortality of third instar grubs. The DutchHeterorhabditis isolate NLH-E87.3 andS. glaseri 326 were selected as candidates for further studies on their potential as biological control agents forP. horticola grubs in the field.     

Species Concepts in the Cylindrocladium floridanum and Cy. spathiphylli Complexes (Hypocreaceae) Based on Multi-allelic Sequence Data, Sexual Compatibility and Morphology

Much attention has recently been devoted to the delimitation of species units in Cylindrocladium(Cy.). In this regard the present study focuses on the taxa within the unresolved Cy. floridanum and Cy. spathiphylli species complexes. Maximum parsimony analyses of DNA sequences of ITS, β-tubulin and histone regions of rRNA genes, and mating experiments revealed a geographically isolated species of Cylindrocladium in the Cy. spathiphylli(teleomorph:Calonectria spathiphylli) species complex.Cy. pseudospathiphylli sp. nov. (teleomorph:Ca. pseudospathiphylli sp. nov.) is described as a new phylogenetic, biological and morphological species. It is distinguished from Cy. spathiphylli by being homothallic, having smaller macroconidia, and distinct DNA sequences of β-tubulin and histone genes. Similarly, parsimony analysis of a combined data set also indicated several phylogenetic species to exist within Cy. floridanum(teleomorph:Ca. kyotensis). Based on differences in vesicle morphology and conidium dimensions, the Canadian population of Cy. floridanum, formerly known as Cy. floridanum Group 2, is described as Cy. canadense sp. nov., while a further collection from Hawaii is described as Cy. pacificum sp. nov.     

Detection of ‘long-haired’ Saprolegnia (S. parasitica) isolates using monoclonal antibodies

The ability of five monoclonal antibodies (Mabs) raised against a pathogenic Saprolegnia parasitica isolate from brown trout to detect and differentiate between isolates with bundles of long hairs (S. parasitica) and other Saprolegnia species was determined by means of an indirect immunofluorescence assay. Four of the Mabs used recognized some of the long-haired S. parasitica isolates but also cross-reacted with other Saprolegnia species without bundles of hairs and with Achlya sp. The other Mab (named 18A6) was able to differentiate between the asexual and most of the sexual isolates in the group of long-haired S. parasitica isolates, but did not recognize Achlya sp. or the Saprolegnia species without bundles of hairs, with the exception of S. hypogyna. These results indicate that isolates with bundles of long hairs are closely related with other members of genus Saprolegnia and share several antigens. However, Mab 18A6 seems to recognize an epitope that is expressed mainly in the asexual isolates in the long-haired S. parasitica isolates.     

Hyperparasitism by Mesochorus spp. (Hymenoptera: Ichneumonidae) in Peristenus sp. (Hymenoptera: Braconidae) and development of PCR primers for hyperparasitoid detection

Peristenus sp. pupae collected from Lygus spp. nymphs in 2001 and 2002 were over-wintered in the laboratory. In both years, more than 30% of adults emerging from over-wintering pupae were identified as ichneumonid hyperparasitoids, Mesochorus curvulus Thomson and Meschorus sp. (Hymenoptera: Ichneumonidae). At the end of the over-wintering period, Peristenus sp. males emerged first followed by Peristenus sp. females and finally Mesochorus spp. The male:female ratio in emerging Peristenus sp. adults was skewed towards males. The Internal Transcribed Spacer (ITS) region and the cytochrome oxidase I (COI) gene from Mesochorus spp. were sequenced. ITS sequences were used to develop PCR primers to detect Mesochorus spp. hyperparasitism in the primary host, Lygus spp. PCR analysis of field-collected Lygus spp. nymphs gave similar estimates of Mesochorus spp. hyperparasitism to the rearing protocols (25–28%). Sequence analysis of COI and ITS regions and subsequent restriction endonuclease analysis of ITS PCR products from Mesochorus spp. indicate the presence of two genotypes in the population. The possibility that these two genotypes represent separate or cyrptic species is discussed.     

Differentiation of Indica-Japonica rice revealed by insertion/deletion (InDel) fragments obtained from the comparative genomic study of DNA sequences between 93-11 ( Indica ) and Nipponbare ( Japonica )

DNA polymorphisms from nucleotide insertion/deletions (InDels) in genomic sequences are the basis for developing InDel molecular markers. To validate the InDel primer pairs on the basis of the comparative genomic study on DNA sequences between an Indica rice 93-11 and a Japonica rice Nipponbare for identifying Indica and Japonica rice varieties and studying wild Oryza species, we studied 49 Indica, 43 Japonica, and 24 wild rice accessions collected from ten Asian countries using 45 InDel primer pairs. Results indicated that of the 45 InDel primer pairs, 41 can accurately identify Indica and Japonica rice varieties with a reliability of over 80%. The scatter plotting data of the principal component analysis (PCA) indicated that: (i) the InDel primer pairs can easily distinguish Indica from Japonica rice varieties, in addition to revealing their genetic differentiation; (ii) the AA-genome wild rice species showed a relatively close genetic relationship with the Indica rice varieties; and (iii) the non-AA genome wild rice species did not show evident differentiation into the Indica and Japonica types. It is concluded from the study that most of the InDel primer pairs obtained from DNA sequences of 93-11 and Nipponbare can be used for identifying Indica and Japonica rice varieties, and for studying genetic relationships of wild rice species, particularly in terms of the Indica-Japonica differentiation. Translated from Journal of Fudan University (Natural Science), 2006, 45(3): 309–315 [译自: 复旦学报(自然科学版)]     

Hypocrella panamensis sp. nov. (Clavicipitaceae, Hypocreales): a new species infecting scale insects on Piper carrilloanum in Panama

A new species, Hypocrella panamensis, is described from collections and cultures obtained on Barro Colorado Island, Panama. In order to aid in placement of this fungus, phylogenetic analyses were conducted using LSU (rDNA) sequences. Hypocrella panamensis is characterized by possessing pulvinate stromata with a Lecanicillium-like anamorphic state and superficial perithecia. Hypocrella panamensis consistently grouped in a clade containing Hypocrella nectrioides, H. phyllogena, and H. africana (100 % PP). Most species of Hypocrella possess Aschersonia or Hirsutella anamorphs. Hypocrella panamensis is unique in the genus Hypocrella in possession of a Lecanicillium-like anamorphic state. In its biological habit Hypocrella panamensis is similar to other species in Hypocrella in that it infects and degrades the scale insect, then grows superficially on nutrients that emerge to the plant surface through the stylet wound.     

Ecology and impact of Allorhogas sp. (Hymenoptera: Braconidae) and Apion sp. (Coleoptera: Curculionoidea) on fruits of Miconia calvescens DC (Melastomataceae) in Brazil

Two fruit-feeding insects, a gall wasp, Allorhogas sp. (Hymenoptera: Braconidae), and a beetle, Apion sp. (Coleoptera: Curculionoidea), were evaluated in their native habitat in Brazil as potential biological control agents of Miconia calvescens DC (Melastomataceae). Allorhogas sp. occurred at two out of three field sites with native populations of M. calvescens, and Apion sp. occurred at all three sites. Both species exhibited aggregated distributions among M. calvescens trees sampled at each site. Allorhogas sp. infested 9.0% and 3.8% of fruits at each of two sites. The number of larvae and pupae of Allorhogas sp. and/or an unidentified parasitoid (Hymenopetera: Eulophidae: Tetrastichinae) ranged from one to five per infested fruit. Fruits infested with Allorhogas sp. were 20% larger and had 79% fewer seeds than healthy fruits. Although adults of Apion sp. were found on leaves and inflorescences of M. calvescens at all three sites, larvae and pupae were found in fruits at only one site, where a maximum of 1.4% of fruits were infested. Fruits infested by Apion sp. contained only one larva or pupa, and were 15% smaller and had 62% fewer seeds than healthy fruits. While a variety of apionids have been used for biological control in the past, this is the first time a braconid wasp has been considered for biological control of a weed.     

四照花亚属的nrDNA ITS致同进化不完全

四照花亚属(Cornus subg.Syncarpea)隶属于山茱萸科山茱萸属(Cornus),我国该亚属共有5种8亚种。为探讨四照花亚属nrDNA ITS序列的致同进化不完全现象及假基因产生的可能原因,分析了该亚属4种(每种1~2个居群)共21个个体的nrDNA ITS序列。结果表明,这些类群的nrDNA ITS存在多态性,通过分析这些nrDNA ITS克隆序列的G+C含量、5.8S保守基序和二级结构最小自由能,推测其可能存在假基因。系统发育研究结果显示所有nrDNA ITS序列分成5个分支,同一个体的不同拷贝被分别置于两个甚至多个分支中,且不同分支显示了不同种间关系。四照花亚属物种个体内部存在nrDNA ITS不完全致同进化,可能归咎于不完全的世系分选(incomplete lineage sorting)、种间杂交或多倍化等进化事件,从而导致基因组内nrITS区序列出现多态性,同时也导致难以通过外部形态来划分亚属内种间界限。     

Hansenomysis anaramosae n. sp., a new suprabenthic mysid (Crustacea: Mysidacea: Petalophthalmidae) from the Bellingshausen Sea (Southern Ocean)

A new petalophthalmid mysid, Hansenomysis anaramosae n. sp., is described from specimens sampled with a suprabenthic sled in the Bellingshausen Sea (Southern Ocean). Hansenomysis anaramosae is clearly distinguishable from its closest congener, H. angusticauda Tattersall, by the carapace sculpturing, the rostrum produced into a spine-like process, the shape and armature of the antennal scale, eyeplate, and telson. This new species is the fourth Hansenomysis species described from the Southern Ocean. A key to these Antarctic species is presented.     

Molecular and morphological characterization of Leveillula (Ascomycota: Erysiphales) on monocotyledonous plants

Leveillula on monocotyledonous plants have been recorded as L. taurica by several authors, whereas the fungus on Allium has been described as an independent species, namely L. allii, by some authors. We sequenced ca 600 bp of the rDNA ITS region for two Leveillula specimens from Allium and Polianthes (both from monocotyledons) and compared them with several already published sequences from Leveillula isolates from dicotyledons. Pair-wise percentages of sequence divergences were calculated for all Leveillula isolates. The ITS sequence of the Polianthes isolate was identical to L. taurica on Helianthus and Vicia. The sequence of the Allium isolate was 99.5 % identical to L. taurica on Euphorbia, Haplophylum, Peganum, etc. These results suggest close relationships between monocot and dicot pathogenic Leveillula species. The identity between two monocot isolates was 98.4 %. Phylogenetic analysis revealed that the two monocot isolates do not group into a clade together. This result suggests that Leveillula acquired parasitism to monocots at least twice independently.     

青藏高原东缘的锈菌资料V.苔草属和姜属植物柄锈菌的增补

本文报告采自四川西部的三种柄锈菌新记录.它们是披针苔草Carex lanceolata Boott上的栗褐苔草柄锈菌Puccinia caricis-brunneae Dietel,苔草Carex sp.上的小堆柄锈菌Pucciniamicrosora Koernicke ex Fuckel以及阳荷Zingiber strialatum Dicls上的姜黄柄锈菌Puccinia curcumae T.S. Ramakrishnan & Sundaram。每个种有简要讨论并附线条图.标本保藏在中国科学院微生物研究所真菌标本室(HMAS)。     

On the taxonomic status of the Antarctic amphipod crustacean genera Eclysis (Astyridae) and Bathypanoploea (Stilipedidae), with partial redescription of their type species and description of Bathypanoploea polarsterni n. sp.

The monotypic genus Eclysis K.H. Barnard, 1932 and its type species, E. similis Barnard, are redescribed based upon a newly discovered second specimen. The genus Bathypanoploea Schellenberg, 1939 is reviewed; B. schellenbergi Holman and Watling, 1983 is fixed as the type species. B. polarsterni n. sp. is described; Alexandrella pulchra Ren in Ren and Huang, 1991 is a new junior synonym of B. schellenbergi. The morphology of Eclysis and Bathypanoploea is examined, as well as their relationships to the Astyridae and Stilipedidae.See also Electronic Supplement at: http://www.senckenberg.de/odes/05-03.htm     

Sorex rohweri sp. nov. (Mammalia, Soricidae) from northwestern North America

Sorex rohweri sp. nov. is described on the basis of a series of specimens from the Olympic Peninsula and adjacent western regions of Washington State, USA, and southwestern British Columbia, Canada. It has been misidentified as Sorex cinereus Kerr, 1792, which occurs in the Cascade Range in west-central Washington, in coastal British Columbia, and regions farther to the northeast. The new species is distinguished from S. cinereus by numerous morphological characters: differences in craniodental dimensions; different location and form of the medial tines of the upper incisors; presence of patent postmandibular foramina; different intensity and distribution of dental pigmentation; and different form of the glans penis, along with other details. The combination of characters also separates it from sympatric species of Sorex. Phylogenetic inference of cytochrome b sequences from two specimens of the proposed new species shows them to be distinct from Sorex cinereus and from five sympatric Sorex species, supporting their designation as members of a new species.     

Bullera begoniae sp. nov. and Bullera setariae sp. nov., two new species of ballistoconidium-forming yeasts in the Bullera variabilis (Bulleribasidium) cluster isolated from plants in Taiwan

Two strains of xylose-containing and Q-10-having ballistoconidiogenous yeasts isolated from plant leaves collected in Taiwan were found to represent two new species of the genus Bullera. In the phylogenetic trees based on the sequence analysis of 18S rDNA and D1/D2 domain of 26S rDNA, these species are located in the Bullera variabilis (Bulleribasidum) cluster in Hymenomycetes. They are described as Bullera begoniae sp. nov. and Bullera setariae sp. nov., respectively.     

秦岭的尾孢菌及其近似属种

摘要:本文报道秦岭地区尾孢菌属及其近似属56种,其中有一个新种:秦岭假尾孢(Pseudocercospora qinlingensis Guo, sp. nov.),寄生在苦木科(Simaroubaceae)臭椿属(Ailanthus sp.)上,两个新组合:草霉色链隔孢[Phaeoramularia vexans (C. Massalongo) Guo,comb. nov.].寄生在蔷薇科(Rosaceae)草霉属(Fragaria sp.)上;篙假尾孢[Pseudocercospora dracunculi (Sarwar) Guo, comb. nov.],寄生在菊科(Compositae)牛尾蒿(Artemisia subdigitataMattf.)上和两个中国新记录种:商陆尾孢(Cercospora pircuniae Speg.),寄生在商陆科(Phytolaccaceae)商陆(Phytolacca acinosa Roxb.)上;荚蒾生尾孢(Cercospora viburnicola Ray).寄生在醉鱼草叶荚蒾(Viburnum buddleifolium C. H. Wight)上.新种(Pseudocercospora qinlingensis Guo, sp. nov.)子实体主要生于叶面,子座沿叶斑边缘发生,暗褐色至近黑色,直径40.0-75.0 (-100.0)μm,分生孢子梗紧密簇生,青黄褐色,不分枝,不呈曲膝状,无隔膜,6.5-26.0 × 3.9-6.0μm,分生孢子圆柱形,青黄褐色至中度褐色,非常弯曲,基部平截,具紧密排列地4至多个隔膜,在隔膜处缴缩,32.0-120.0 (-150.0) ×4.0-6.5 (-7.6)μm.新种与寄生在臭椿属(Ailanthus spp.)植物上的臭椿尾孢(Cercospora ailanthi P. Henn.)和臭椿生假尾孢[Pseudocercospora ailanthicola(Patwardhan) Dcighton]不同:臭椿尾孢无子座.分生孢子梗不成簇,分枝,长(50.0-200.0μm),分生孢子倒棍棒一圆柱形,色泽浅(浅青黄褐色),隔膜少(5-7隔),不缢缩,短(15.0-60.0μm);臭椿生假尾孢子实体叶背生,分生孢子梗窄((3.0-4.Spm),分生孢子倒棍棒形,隔膜少((5-8隔),不绣缩,短而窄(20.0-63.0 ×3.0-5.0μm).     

Evaluation of certain entomopathogenic nematodes against the melon ladybird,Epilachna chrysomelina F. (Coleoptera: Coccinellidae)

Three native Egyptian nematode isolates; Heterorhabditis taysearae and Heterorhabditis sp. S1 (Heterorhabditidae) and Steinernema carpocapsae S2 (Steinernematidae) as well as H. bacteriophora Hp88 as an imported species, were used in the present work to evaluate their activities against larvae and adults of the melon ladybird, Epilachna chrysomelina. The target pest was found to be susceptible to all tested entomopathogenic nematodes under laboratory conditions of 30±5°C.

In the greenhouse, a single spray of nematode suspension (1000 infective juveniles per ml) of each of H. taysearae, H. bacteriophora Hp88 and Steinernema carpocapsae S2 on squash seedlings was enough to give a reasonable mortality of 4^th larval instar E. chrysomelina, reaching 65.2, 44.0 and 84.0%, respectively, one week after application. This gives evidence that the Egyptian nematode isolates could tolerate high temperature and could be recommended for application in the control programmes of E. chrysomelina larvae in cucurbit fields.     

中国单格孢属（丝孢纲）的种

报道单格孢属的两个新种,一个新组合,5个中国新记录种和两个未定名种,同时,对所有已知在中国发现的本属真菌的种进行了归纳总结并制作了检索表。新种小黑球单格孢Monodictys nigraglobulosa的典型特征是产生黑色近球形的分生孢子,具有黑而厚的隔膜;横向单格孢Monodictys transversa的分生孢子横向生长,即孢子的横向量度大于其高度。两个未定名种具有单格孢属的特征,但目前的材料所表现的分种特征还不够充分,暂称之为Monodictys sp.1和 Monodictys sp.2,供进一步研究探讨。新组合为方单格孢Monodictys quadrata (Atk.) G. Z. Zhao & T. Y. Zhang。研究标本保存在山东农业大学植物病理学标本室（HSAUP）。     

Prosthecium species with Stegonsporium anamorphs on Acer

Data from microscopic morphology, single-spore cultures, and DNA analyses of teleomorphs and anamorphs support the recognition of five species of Prosthecium with Stegonsporium anamorphs on Acer: P. acerinum sp. nov., the teleomorph of S. acerinum; P. acerophilum comb. nov., formerly known as Dictyoporthe acerophila; P. galeatum comb. nov., originally described as Massaria galeata; P. opalus sp. nov.; and P. pyriforme sp. nov., the teleomorph of S. pyriforme s. str. The morphology of both type specimens and freshly collected material was investigated. The teleomorphs have brown ellipsoidal ascospores with five distosepta and often a longitudinal distoseptum. The anamorphs of all species described here belong to Stegonsporium; their connection to the Prosthecium teleomorphs was demonstrated by morphology and DNA sequences of single spore cultures derived from both ascospores and conidia. The anamorphs and teleomorphs of all five Prosthecium species are described and illustrated by LM images, and a key to these species is provided. As perceived from this work, S. pyriforme is restricted to Europe and does not occur in North America, whereas S. acerinum is restricted to North America, not found in Europe. The host associations given in the literature are revised and evidence is provided that only A. opalus, A. pseudoplatanus, and A. saccharum are confirmed hosts of Prosthecium with Stegonsporium anamorphs. Molecular phylogenetic analyses of tef1, ITS rDNA, and partial nuLSU rDNA sequences confirm that the species with Stegonsporium anamorphs are closely related to P. ellipsosporum, the generic type species. Stilbospora macrosperma is confirmed as the anamorph of P. ellipsosporum by DNA data of single spore isolates obtained from both ascospores and conidia.     

石耳属一新种(子囊菌门)

本文描述了地衣型石耳属一新种,周鳞石耳.新种脐叶体上表面以周边皮层碎片上翘而形成的大量准鳞芽,类似于淡腹鳞石耳,但是,不同之处在于新种脐叶体下表面黑色,覆以大量同色而多分枝的假根以及具有不同的地理分布.从而呈现为地理替代现象.文中为新种提供了拉丁文特征提要、英文描述、图片与地理分布图以及新种及其相关种的检索表.