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1.
Elucidating protein translational regulation is crucial for understanding cellular function and drug development. A key molecule in protein translation is ribosome, which is a super-molecular complex extensively studied for more than a half century. The structure and dynamics of ribosome complexes were resolved recently thanks to the development of X-ray crystallography, Cryo-EM, and single molecule biophysics. Current studies of the ribosome have shown multiple functional states, each with a unique conformation. In this study, we analyzed the RNA-protein distances of ribosome (2.5 MDa) complexes and compared these changes among different ribosome complexes. We found that the RNA-protein distance is significantly correlated with the ribosomal functional state. Thus, the analysis of RNA-protein binding distances at important functional sites can distinguish ribosomal functional states and help understand ribosome functions. In particular, the mechanism of translational attenuation by nascent peptides and antibiotics was revealed by the conformational changes of local functional sites. 相似文献
2.
Hauryliuk VV 《Molekuliarnaia biologiia》2006,40(5):769-783
Four protein factors, belonging to the GTPase superfamily, participate in bacterial biosynthesis: IF2, EF-G, EF-Tu and RF3. The exact role and mechanism of action of these proteins was of particular interest over the last several decades. Recent advances in structural methods of ribosomal research, especially application of cryoelectron microscopy, provided powerful experimental tools for the investigation of ribosomal dynamics during translation. Simultaneously, progress in the biochemical investigation of translation allowed us to link structural rearrangements occurring in the ribosome to functional changes in the ribosome-bound translational GTPases--GDP/GTP exchange, GTPase activation and its conformational changes. Accumulated data have lead to formulation of current models of mechanisms of translation. More and more facts testify in favor of the idea that the ribosome plays a prominent role both in the nucleotide exchange and in GTPase activation, thus playing the role both of GAP and GEF for RF3, IF2 and EF-G. In our work we attempted to systematize the most important experimental findings and models for mechanisms of GTPases function and regulation in prokaryotic translation. 相似文献
3.
Al-Karadaghi S Kristensen O Liljas A 《Progress in biophysics and molecular biology》2000,73(2-4):167-193
The key reaction of protein synthesis, peptidyl transfer, is catalysed in all living organisms by the ribosome - an advanced and highly efficient molecular machine. During the last decade extensive X-ray crystallographic and NMR studies of the three-dimensional structure of ribosomal proteins, ribosomal RNA components and their complexes with ribosomal proteins, and of several translation factors in different functional states have taken us to a new level of understanding of the mechanism of function of the protein synthesis machinery. Among the new remarkable features revealed by structural studies, is the mimicry of the tRNA molecule by elongation factor G, ribosomal recycling factor and the eukaryotic release factor 1. Several other translation factors, for which three-dimensional structures are not yet known, are also expected to show some form of tRNA mimicry. The efforts of several crystallographic and biochemical groups have resulted in the determination by X-ray crystallography of the structures of the 30S and 50S subunits at moderate resolution, and of the structure of the 70S subunit both by X-ray crystallography and cryo-electron microscopy (EM). In addition, low resolution cryo-EM models of the ribosome with different translation factors and tRNA have been obtained. The new ribosomal models allowed for the first time a clear identification of the functional centres of the ribosome and of the binding sites for tRNA and ribosomal proteins with known three-dimensional structure. The new structural data have opened a way for the design of new experiments aimed at deeper understanding at an atomic level of the dynamics of the system. 相似文献
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核糖体是蛋白质的"合成工厂",也是临床上多种抗菌药物的作用靶点,因此,深入理解细菌核糖体的蛋白质翻译机制意义重大.蛋白质翻译是通过多步骤相互协调、多组分精细配合来实现高保真和精确调控.核糖体在mRNA上的移位作为翻译过程中最重要的事件之一,需要核糖体大规模的构象重排以及tRNA2-mRNA沿着核糖体的精确移动.在细菌中,移位是由延伸因子EF-G催化GTP水解来驱动的.近年来,单分子荧光共振能量技术(smFRET)的发展使得人们可以探究单个tRNA分子移位的动力学过程并实时观测核糖体的构象变化.本文首先介绍了smFRET技术的原理及特点,对其在核糖体结构动态及tRNA移位研究中的应用进行了较为系统的总结,并对其应用前景进行了展望. 相似文献
6.
During protein synthesis, mRNA and tRNA are moved through the ribosome by the process of translocation. The small diameter of the mRNA entrance tunnel only permits unstructured mRNA to pass through. However, there are structured elements within mRNA that present a barrier for translocation that must be unwound. The ribosome has been shown to unwind RNA in the absence of additional factors, but the mechanism remains unclear. Here, we show using single molecule Förster resonance energy transfer and small angle X‐ray scattering experiments a new global conformational state of the ribosome. In the presence of the frameshift inducing dnaX hairpin, the ribosomal subunits are driven into a hyper‐rotated state and the L1 stalk is predominantly in an open conformation. This previously unobserved conformational state provides structural insight into the helicase activity of the ribosome and may have important implications for understanding the mechanism of reading frame maintenance. 相似文献
7.
Eukaryotic translation initiation factors and regulators 总被引:18,自引:0,他引:18
Significant progress has been made over the past several years on structural studies of the eukaryotic translation initiation factors that facilitate the assembly of a translation-competent ribosome at the initiation codon of an mRNA. These structural studies have revealed the repeated use of a set of common structural folds, highlighted the evolutionary conservation of the translation apparatus, and provided insight into the mechanism and regulation of cellular and viral protein synthesis. 相似文献
8.
《Cell cycle (Georgetown, Tex.)》2013,12(20):3441-3446
Ribosome biogenesis and translation can be simplified as the processes of generating ribosomes and their use for decoding mRNA into a protein. Ribosome biogenesis has been efficiently studied in unicellular organisms like the budding yeast, allowing us a deep and basic knowledge of this process in growing cells. Translation has been modeled in vitro and in unicellular organisms. These studies have given us an important insight into the mechanisms and evolutionarily conserved aspects of ribosome biology. However, we advocate the need of the direct study of these processes in multicellular organisms. Analysis of ribosome biogenesis and translation in vivo in Metazoa and mammalian models is emerging and unveils the unexpected consequences of perturbed ribosome biogenesis and translation. Here, we will describe how one factor, eIF6, plays a crucial role both in the generation of the large ribosomal subunit and its availability for translation. From there, we will make specific conclusions on the physiological relevance of eIF6 in 80S formation, cell cycle progression and disease, raising the point that the control of gene expression may occur at the unexpected level of the large ribosomal subunit. In the future, the modulation of eIF6 binding to the 60S may be pharmacologically exploited to reduce the growth of cancer cells or ameliorate the phenotype of SDS syndrome. 相似文献
9.
G. I. Makarov T. M. Makarova N. V. Sumbatyan A. A. Bogdanov 《Biochemistry. Biokhimii?a》2016,81(13):1579-1588
The ribosome as a complex molecular machine undergoes significant conformational changes while synthesizing a protein molecule. Molecular dynamics simulations have been used as complementary approaches to X-ray crystallography and cryoelectron microscopy, as well as biochemical methods, to answer many questions that modern structural methods leave unsolved. In this review, we demonstrate that all-atom modeling of ribosome molecular dynamics is particularly useful in describing the process of tRNA translocation, atomic details of behavior of nascent peptides, antibiotics, and other small molecules in the ribosomal tunnel, and the putative mechanism of allosteric signal transmission to functional sites of the ribosome. 相似文献
10.
Accessibility of 18S rRNA in human 40S subunits and 80S ribosomes at physiological magnesium ion concentrations--implications for the study of ribosome dynamics
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Protein biosynthesis requires numerous conformational rearrangements within the ribosome. The structural core of the ribosome is composed of RNA and is therefore dependent on counterions such as magnesium ions for function. Many steps of translation can be compromised or inhibited if the concentration of Mg(2+) is too low or too high. Conditions previously used to probe the conformation of the mammalian ribosome in vitro used high Mg(2+) concentrations that we find completely inhibit translation in vitro. We have therefore probed the conformation of the small ribosomal subunit in low concentrations of Mg(2+) that support translation in vitro and compared it with the conformation of the 40S subunit at high Mg(2+) concentrations. In low Mg(2+) concentrations, we find significantly more changes in chemical probe accessibility in the 40S subunit due to subunit association or binding of the hepatitis C internal ribosomal entry site (HCV IRES) than had been observed before. These results suggest that the ribosome is more dynamic in its functional state than previously appreciated. 相似文献
11.
Lindqvist L Oberer M Reibarkh M Cencic R Bordeleau ME Vogt E Marintchev A Tanaka J Fagotto F Altmann M Wagner G Pelletier J 《PloS one》2008,3(2):e1583
RNA helicases represent a large family of proteins implicated in many biological processes including ribosome biogenesis, splicing, translation and mRNA degradation. However, these proteins have little substrate specificity, making inhibition of selected helicases a challenging problem. The prototypical DEAD box RNA helicase, eIF4A, works in conjunction with other translation factors to prepare mRNA templates for ribosome recruitment during translation initiation. Herein, we provide insight into the selectivity of a small molecule inhibitor of eIF4A, hippuristanol. This coral-derived natural product binds to amino acids adjacent to, and overlapping with, two conserved motifs present in the carboxy-terminal domain of eIF4A. Mutagenesis of amino acids within this region allowed us to alter the hippuristanol-sensitivity of eIF4A and undertake structure/function studies. Our results provide an understanding into how selective targeting of RNA helicases for pharmacological intervention can be achieved. 相似文献
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Alexey Petrov Jin Chen Seán O’Leary Albert Tsai Joseph D. Puglisi 《Cold Spring Harbor perspectives in biology》2012,4(9)
Decades of extensive biochemical and biophysical research have outlined the mechanism of translation. Rich structural studies have provided detailed snapshots of the translational machinery at all phases of the translation cycle. However, the relationship between structural dynamics, composition, and function remains unknown. The multistep nature of each stage of the translation cycle results in rapid desynchronization of individual ribosomes, thus hindering elucidation of the underlying mechanisms by conventional bulk biophysical and biochemical methods. Single-molecule approaches unsusceptible to these complications have led to the first glances at both compositional and conformational dynamics on the ribosome and their impact on translational control. These experiments provide the necessary link between static structure and mechanism, often providing new perspectives. Here we review recent advances in the field and their relationship to structural and biochemical data.Translation and its regulation are intrinsically dynamic processes. In all organisms, to initiate translation, ribosomes must assemble from isolated subunits and an initiator transfer RNA (tRNA) on a messenger RNA (mRNA) at a specific start codon to establish a reading frame; protein factors guide this process. Elongation occurs through selection by the ribosome of cognate aminoacyl tRNAs, subsequent positioning of tRNAs for peptide bond formation chemistry, and movements of the tRNAs and mRNAs with respect to the codon (translocation). The directional process is iterative until termination at a stop codon, where the protein chain is released, and the ribosomal particle disassembled and recycled. Multiple ribosomes form higher-order polysomes on a single mRNA, with their own intrinsic dynamics.Dynamics are central to the mechanism and control of translation. Here we explicitly define dynamics as time-dependent changes in either composition or conformation of the translational machinery. Conformational dynamics in chemical systems are governed by an array of processes with vastly different timescales. Generally, dynamic processes become slower as they involve larger numbers of atoms. These range from electronic motions (timescale 10−14 sec), bond vibrations (10−13–10−12 sec), through protein side chain or nucleic acid base/sugar local conformational changes (10−11–10−6 sec), to larger conformational rearrangements (domain movements, etc.; 10−6–102 sec) that are often functionally cooperative. Compositional dynamics are determined by bimolecular association and dissociation rate constants: bimolecular arrival rates for ligands are governed by intermolecular collision frequencies, electrostatic interactions, and proper binding orientations for productive binding events, whereas dissociation rates are governed by energy barriers for dissociation of noncovalent intermolecular interactions.Fluctuations in molecular conformation and composition must be harnessed by the ribosome for accurate and rapid translation. The timescales of these dynamic changes dictate the overall rates of translation initiation and elongation: 0.2–0.5 initiation events/sec and elongation rates of 20–40 amino acids/sec in vivo, 1–5 amino acids/sec in vitro (Dennis and Bremer 1974a,b; Underwood et al. 2005). The ribosome uses external sources of free energy during translation—ATP hydrolysis during eukaryotic scanning, GTP hydrolysis by initiation, elongation and termination factors, and peptide bond formation. The free energy released by these irreversible reactions is used to drive the fidelity of initiation and elongation and the directional movement of the ribosome during both processes. The ribosome is thus a molecular motor.The link between ribosome and ligand dynamics and the control of protein synthesis remains a key mystery of translation. The past decade has witnessed the three-dimensional structures of prokaryotic, archaeal, and eukaryotic ribosomes at atomic resolution. How factors, tRNA, and ligands interact with the ribosome has been revealed by cryo-electron microscopy (cryo-EM) (at lower 6–12 Å resolution) and X-ray diffraction studies (as low as 2.5 Å for 30S from Thermus thermophilus [Kurata et al. 2008] and 2.4 Å for 50S from Haloarcula marismortui [Ban et al. 2000]). These structures have shown how GTPase factors engage with the 70S ribosomes at a conserved factor-binding site on the large subunit to mediate GTPase activity and subsequent conformational changes. Another key observation of early cryo-EM and more recent structural studies is that the ribosome adopts two general intersubunit conformations, related by a 6° rotation of the two subunits (Valle et al. 2003; Schuwirth et al. 2005; Agirrezabala et al. 2008; Zhang et al. 2009; Fischer et al. 2010; Dunkle et al. 2011). Peptide bond formation leads to a counterclockwise rotation of the small subunit with respect to the large subunit, and EF-G in the GTP form binds to this state. The peripheral domain L1 region of the ribosome was observed structurally to change its state in correlation with the two ribosomal conformations (Valle et al. 2003; Schuwirth et al. 2005; Agirrezabala et al. 2008), suggesting a coupling of domains within the ribosome. The intersubunit conformation of the ribosome was also correlated by EM to the relative orientations of tRNAs: In the nonrotated state (locked conformation), tRNAs are observed in the classical P site and A site, whereas in the rotated state (unlocked conformation) the tRNAs are in the Noller hybrid states with the 3′ ends of the tRNAs moved to the E and P sites and their respective anticodons in the P and A sites (Agirrezabala et al. 2008). These static structural views suggested a further correlation of tRNA and ribosome conformation during translation.The structural snapshots and prior biochemical studies are suggestive of dynamics during translation, yet experimental methods with resolution in real time are required to observe them directly. Here we focus on single-molecule methodologies that have provided an unprecedented view into the dynamics of prokaryotic translation. In the future, the same techniques can be applied to the study of eukaryotic translation dynamics. 相似文献
14.
When the export of E. coli SecM is blocked, a 17 amino acid motif near the C terminus of the protein induces a translation arrest from within the ribosome tunnel. Here we used a recently described application of fluorescence resonance energy transfer (FRET) to gain insight into the mechanism of translation arrest. We found that the SecM C terminus adopted a compact conformation upon synthesis of the arrest motif. This conformational change did not occur spontaneously, but rather was induced by the ribosome. Translation arrest required both compaction of the SecM C terminus and the presence of key residues in the arrest motif. Further analysis showed that the arrested peptidyl-tRNA was resistant to puromycin treatment and revealed additional changes in the ribosome-nascent SecM complex. Based on these observations, we propose that translation arrest results from a series of reciprocal interactions between the ribosome and the C terminus of the nascent SecM polypeptide. 相似文献
15.
Ping Xie 《European biophysics journal : EBJ》2013,42(5):347-354
A ribosome is an enzyme that catalyzes translation of the genetic information encoded in messenger RNA (mRNA) into proteins. Besides translation through the single-stranded mRNA, the ribosome is also able to translate through the duplex region of mRNA via unwinding the duplex. Here, based on our proposed ribosome translation model, we study analytically the dynamics of Escherichia coli ribosome translation through the duplex region of mRNA, and compare with the available single molecule experimental data. It is shown that the ribosome uses only one active mechanism (mechanical unwinding), rather than two active mechanisms (open-state stabilization and mechanical unwinding), as proposed before, to unwind the duplex. The reduced rate of translation through the duplex region is due to the occurrence of futile transitions, which are induced by the energy barrier from the duplex unwinding to the forward translocation along the single-stranded mRNA. Moreover, we also present predicted results of the average translation rate versus the external force acting on the ribosome translating through the duplex region and through the single-stranded region of mRNA, which can be easily tested by future experiments. 相似文献
16.
《Journal of molecular biology》2023,435(15):168185
Mediated by elongation factor G (EF-G), ribosome translocation along mRNA is accompanied by rotational movement between ribosomal subunits. Here, we reassess whether the intersubunit rotation requires GTP hydrolysis by EF-G or can occur spontaneously. To that end, we employ two independent FRET assays, which are based on labeling either ribosomal proteins (bS6 and bL9) or rRNAs (h44 of 16S and H101 of 23S rRNA). Both FRET pairs reveal three FRET states, corresponding to the non-rotated, rotated and semi-rotated conformations of the ribosome. Both FRET assays show that in the absence of EF-G, pre-translocation ribosomes containing deacylated P-site tRNA undergo spontaneous intersubunit rotations between non-rotated and rotated conformations. While the two FRET pairs exhibit largely similar behavior, they substantially differ in the fraction of ribosomes showing spontaneous fluctuations. Nevertheless, instead of being an invariable intrinsic property of each FRET pair, the fraction of spontaneously fluctuating molecules changes in both FRET assays depending on experimental conditions. Our results underscore importance of using multiple FRET pairs in studies of ribosome dynamics and highlight the role of thermally-driven large-scale ribosome rearrangements in translation. 相似文献
17.
Hepatitis C virus uses an internal ribosome entry site (IRES) to control viral protein synthesis by directly recruiting ribosomes to the translation-start site in the viral mRNA. Structural insights coupled with biochemical studies have revealed that the IRES substitutes for the activities of translation-initiation factors by binding and inducing conformational changes in the 40S ribosomal subunit. Direct interactions of the IRES with initiation factor eIF3 are also crucial for efficient translation initiation, providing clues to the role of eIF3 in protein synthesis. 相似文献
18.
Miljan Simonović Thomas A. Steitz 《Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms》2009,1789(9-10):612-623
The ribosome is a large ribonucleoprotein particle that translates genetic information encoded in mRNA into specific proteins. Its highly conserved active site, the peptidyl-transferase center (PTC), is located on the large (50S) ribosomal subunit and is comprised solely of rRNA, which makes the ribosome the only natural ribozyme with polymerase activity. The last decade witnessed a rapid accumulation of atomic-resolution structural data on both ribosomal subunits as well as on the entire ribosome. This has allowed studies on the mechanism of peptide bond formation at a level of detail that surpasses that for the classical protein enzymes. A current understanding of the mechanism of the ribosome-catalyzed peptide bond formation is the focus of this review. Implications on the mechanism of peptide release are discussed as well. 相似文献
19.
We have probed the structure and dynamics of ribosomal RNA in the Escherichia coli ribosome using equilibrium and time-resolved hydroxyl radical (OH) RNA footprinting to explore changes in the solvent-accessible surface of the rRNA with single-nucleotide resolution. The goal of these studies is to better understand the structural transitions that accompany association of the 30 S and 50 S subunits and to build a foundation for the quantitative analysis of ribosome structural dynamics during translation. Clear portraits of the subunit interface surfaces for 16 S and 23 S rRNA were obtained by constructing difference maps between the OH protection maps of the free subunits and that of the associated ribosome. In addition to inter-subunit contacts consistent with the crystal structure, additional OH protections are evident in regions at or near the subunit interface that reflect association-induced conformational changes. Comparison of these data with the comparable difference maps of the solvent-accessible surface of the rRNA calculated for the Thermus thermophilus X-ray crystal structures shows extensive agreement but also distinct differences. As a prelude to time-resolved OH footprinting studies, the reactivity profiles obtained using Fe(II)EDTA and X-ray generated OH were comprehensively compared. The reactivity patterns are similar except for a small number of nucleotides that have decreased reactivity to OH generated from Fe(II)EDTA compared to X-rays. These nucleotides are generally close to ribosomal proteins, which can quench diffusing radicals by virtue of side-chain oxidation. Synchrotron X-ray OH footprinting was used to monitor the kinetics of association of the 30 S and 50 S subunits. The rates individually measured for the inter-subunit contacts are comparable within experimental error. The application of this approach to the study of ribosome dynamics during the translation cycle is discussed. 相似文献
20.
Gao H Sengupta J Valle M Korostelev A Eswar N Stagg SM Van Roey P Agrawal RK Harvey SC Sali A Chapman MS Frank J 《Cell》2003,113(6):789-801
Cryo-EM density maps showing the 70S ribosome of E. coli in two different functional states related by a ratchet-like motion were analyzed using real-space refinement. Comparison of the two resulting atomic models shows that the ribosome changes from a compact structure to a looser one, coupled with the rearrangement of many of the proteins. Furthermore, in contrast to the unchanged inter-subunit bridges formed wholly by RNA, the bridges involving proteins undergo large conformational changes following the ratchet-like motion, suggesting an important role of ribosomal proteins in facilitating the dynamics of translation. 相似文献