共查询到20条相似文献,搜索用时 31 毫秒
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Ohnishi Y Yamazaki H Kato JY Tomono A Horinouchi S 《Bioscience, biotechnology, and biochemistry》2005,69(3):431-439
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Babu M Greenblatt JF Emili A Strynadka NC Reithmeier RA Moraes TF 《Structure (London, England : 1993)》2010,18(11):1450-1462
Escherichia coli YchM is a member of the SLC26 (SulP) family of anion transporters with an N-terminal membrane domain and a C-terminal cytoplasmic STAS domain. Mutations in human members of the SLC26 family, including their STAS domain, are linked to a number of inherited diseases. Herein, we describe the high-resolution crystal structure of the STAS domain from E. coli YchM isolated in complex with acyl-carrier protein (ACP), an essential component of the fatty acid biosynthesis (FAB) pathway. A genome-wide genetic interaction screen showed that a ychM null mutation is synthetically lethal with mutant alleles of genes (fabBDHGAI) involved in FAB. Endogenous YchM also copurified with proteins involved in fatty acid metabolism. Furthermore, a deletion strain lacking ychM showed altered cellular bicarbonate incorporation in the presence of NaCl and impaired growth at alkaline pH. Thus, identification of the STAS-ACP complex suggests that YchM sequesters ACP to the bacterial membrane linking bicarbonate transport with fatty acid metabolism. 相似文献
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Birkó Z Bialek S Buzás K Szájli E Traag BA Medzihradszky KF Rigali S Vijgenboom E Penyige A Kele Z van Wezel GP Biró S 《Molecular & cellular proteomics : MCP》2007,6(7):1248-1256
Members of the prokaryotic genus Streptomyces produce over 60% of all known antibiotics and a wide range of industrial enzymes. A leading theme in microbiology is which signals are received and transmitted by these organisms to trigger the onset of morphological differentiation and antibiotic production. The small gamma-butyrolactone A-factor is an important autoregulatory signaling molecule in streptomycetes, and A-factor mutants are blocked in development and antibiotic production. In this study we showed that heterologous expression of the 324-amino acid secreted regulatory protein Factor C resulted in restoration of development and enhanced antibiotic production of an A-factor-deficient bald mutant of Streptomyces griseus, although the parental strain lacks an facC gene. Proteome analysis showed that in the facC transformant the production of several secreted proteins that belong to the A-factor regulon was restored. HPLC-MS/MS analysis indicated that this was due to restoration of A-factor production to wild-type levels in the transformant. This indicates a connection between two highly divergent types of signaling molecules and possible interplay between their regulatory networks. 相似文献
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Molecular structure and translocation of a multiple antibiotic resistance region of a Psychrobacter psychrophilus permafrost strain 总被引:1,自引:0,他引:1
A Psychrobacter psychrophilus strain resistant to tetracycline and streptomycin was isolated from a 15 000–35 000-year-old permafrost subsoil sediment sampled from the coast of the Eastern-Siberian Sea. The genes conferring antibiotic resistance were localized on an c . 30-kb pKLH80 plasmid. It was shown that the antibiotic resistance region of this plasmid has a mosaic structure and contains closely linked streptomycin resistance ( strA-strB ) and tetracycline resistance [ tetR-tet (H)] genes, followed by a novel IS element (IS Ppy1 ) belonging to the IS 3 family. Both the strA-strB and tetR-tet (H) genes of pKLH80 were highly similar to those found in modern clinical bacterial isolates. It was shown that the IS Ppy1 element of pKLH80 can direct translocation of the adjacent antibiotic resistance genes to different target plasmids, either by one-ended transposition or by formation of a composite transposon resulting from the insertion of the IS Ppy1 second copy at the other side of the antibiotic resistance region. Thus, our data demonstrate that clinically important antibiotic resistance genes originated long before the introduction of antibiotics into clinical practice and confirm an important role of horizontal gene transfer in the distribution of these genes in natural bacterial populations. 相似文献
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Cundliffe E 《Journal of industrial microbiology & biotechnology》2006,33(7):500-506
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V D Gruzina O V Efremenkova V A Zenkova M I Reznikova Iu V Dudnik 《Antibiotiki i khimioterapii͡a》2003,48(3):11-16
It was shown the stimulating role of A-factor on soil prokaryotes growth. Soil sample culturing on agar medium, containing A-factor, resulted in the colony forming units (CFU) increasing in comparison with culturing on the medium without this regulator. Gram-negative bacteria were the reason of CFU increasing; previously the effect of A-factor on bacteria of this group was not shown. Gram-negative rod bacterial strain No. 35 was isolated and shown that CFU number was approximately twice increased at A-factor concentration in agar medium 2 and 7 mcg/ml; high A-factor concentration up to 28 mcg/ml was not effective. Isolated strain No. 35 is the producer of antibiotic, effective against gram-positive bacteria. 相似文献
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A putative two-component regulatory system involved in secondary metabolism in Streptomyces spp. 总被引:7,自引:0,他引:7 下载免费PDF全文
H Ishizuka S Horinouchi H M Kieser D A Hopwood T Beppu 《Journal of bacteriology》1992,174(23):7585-7594
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Crystal structure of nonphosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli 下载免费PDF全文
Ekaterina V. Filippova Zdzislaw Wawrzak Jiapeng Ruan Sergii Pshenychnyi Richard M. Schultz Alan J. Wolfe Wayne F. Anderson 《Protein science : a publication of the Protein Society》2016,25(12):2216-2224