雪茶化学成分研究

由管枝衣科地茶属植物雪茶（Thamnolia vermicularis)丙酮提取物中分离得到10个成分,通过波谱分析及与已知品对照等方法,最终确定其中的9个化合物分别为:Thamnolin(1),鳞片衣酸（2）,坝巴酸（3）,羊角衣酸（4）,3β－羟基－5α,8α－桥二氧麦角甾－6,22－二烯（5）,3β－羟基－5α,8α－桥二氧麦角甾－6,9,22－三烯（6）,麦角甾烷－7,22－二烯－3－醇（7）,麦角甾烷－5,8,22－三烯－3－醇（8）,亚油酸(9)。其中,化合物1为新化合物,化合物3和6－9系首次由该种植物中分离得到。研究结果初步明确了云南中甸地区产雪茶为主要含有坝巴酸（3）和羊角衣酸（4）等酚性成分,而非主含地茶酸等酚性成分的植物品种类型,这为合理开发利用当地植物资源提供了科学依据。     

蒙椴树皮化学成分的研究

为研究蒙椴（Tilia mongolica Maxim.）树皮的化学成分,通过溶剂萃取、硅胶柱色谱、重结晶分离纯化,从蒙椴树皮中分离得到7个化合物,根据其红外光谱、质谱和核磁共振谱数据鉴定为β-香树脂醇乙酸酯（1）,计曼尼醇乙酸酯（2）,羽扇豆醇（3）,二十四碳酸（4）,三棕榈酸甘油酯（5）,β-谷甾醇（6）,胡萝卜苷（7）。其中化合物1-5、7均为首次从椴树属植物中分离得到,化合物6为首次从该植物中分离得到。     

紫花列当水溶性成分的研究

采用大孔树脂、SephadexLH-20等色谱方法,从列当科列当属植物紫花列当Orobanche coerulescens中分离得到六个水溶性成分,通过波谱技术及理化手段分别鉴定为类叶升麻苷（1）、异类叶升麻苷（2）、crenatoside（3）、cistanoside F（4）、sinapoyl-4—O-β-D-glucoside（5）、腺苷（6）。其中化合物5和6为首次从该属植物中分离得到。     

白花蛇舌草化学成分的研究

采用聚酰胺、Sepphadex LH-20、硅胶等多种色谱柱对白花蛇舌草的化学成分进行分离纯化,并根据化合物的理化性质和光谱数据鉴定其结构为：去乙酰车叶草甙（1）、车叶草甙（2）、鸡屎藤甙甲酯（3）、车叶草酸（4）、去乙酰车叶草酸甲酯（5）和dapbylloside（6）。其中化合物6为首次从该属植物中分离得到,化合物1,5、6为首次从该种植物中得到。     

灯盏花中的糖苷

从灯盏花（Erigeron breviscapus (Vant.) Hand.-Mazz.) 全株的乙醇提取物的正丁醇部分分得10个化合物,通过波谱方法鉴定为erigeside I(1)、γ－吡喃酮－3－O－β－D－[6′－（4″－羟基－3″,5″－二甲氧基苯甲酰）-]-吡喃葡萄糖苷（erigeside D,2)、erigeside A(3)、erigeside B(4)、erigeside Ⅱ(5)、7-O-β-D-吡喃葡萄糖－6－甲氧基香豆素（6）、icariside B2(7)、blumenol C葡萄糖苷（8）、（＋）－丁香树酯醇O－β－D－吡喃葡萄糖苷（9）和野黄芩苷甲酯（10）。用X射线单晶衍射方法确定了化合物7的绝对构型。2是一个新化合物,7、8和9首次从该属植物分离得到,6首次从该种植物分离得到。     

崖椒茎的化学成分

从崖椒（Zanthoxglum schinifolutm Sieb.et Zucc.)茎的石油醚、二氯甲烷提取物中分离得到8个化合物。经物理常数测定及光谱（UV,IR,MS,NMR)分析鉴定其为（1）白鲜碱（dictamning),（2）茵芋碱（skimmianine),(3)滨蒿内酯（scoparone),(4)崖椒内酯（schinifolin),(5)莨菪亭（scopoletin),（6）7-羟基-8-甲氧基香豆素（7-hydroxy-8-methoxycoumarin),（7）N-甲基弗林辛（N-methylflindersine),（8）β-谷甾醇（β-sitosterol),其中化合物（5）、（6）和（7）为首次从该植物中分离。     

蓝桉果实中的脂溶性成分

从蓝桉(Eucalyptus globulusLabill.)果实乙醇提取物的石油醚部位分离鉴定出6个化合物,通过理化和波谱分析结构鉴定为:5-羟基-4′,7-二甲氧基-6-甲基黄酮(1)、blumenol A(2)、蓝桉醇(3)、绿花白千层醇(4)、β-谷甾醇(5)、二十二烷酸(6),其中化合物1~4和化合物6系首次从该植物中分离得到,化合物1和2首次从桉属植物中分离得到.     

无梗五加根中苯丙素类化合物的研究

从无梗五加(Acanthoparax sessiliflorus( Rupr.et Maxim.)Seem.)根70％乙醇提取物的乙酸乙酯层中提取分离得到8个苯丙素类化合物.经理化和波谱分析鉴定为(+)-表芝麻脂素(1)、(-)-芝麻脂素(2)、赛菊芋黄素(3)、洒维宁(4)、咖啡酸甲酯(5)、对羟基桂皮酸(6)、(-)-丁香脂素(7)、(+)-松脂索(8).化合物8是首次从五加科植物中分离得到,化合物1和5是首次从五加属植物中分离得到,化合物3、6和7是首次从该植物中分离得到.     

遍地金的化学成分研究Ⅱ

从遍地金(Hypericum wightianum Wall．ex Wight et Am．)乙醇提取物中分离得到3个化合物,经波谱分析鉴定为6-deoxyisojacareubin(1),6-异戊烯基槲皮素-3-甲醚(2)和香草酸(3)。3个化合物均为首次从该植物中分离得到。     

显齿蛇葡萄(藤茶)化学成分研究

从显齿蛇葡萄的乙酸乙酯提取物中分离得到9个化合物,经波谱分析、化学分析及与已知化合物对照,分别鉴定为：棕桐酸（1）、β－谷甾醇（2）、大黄素（3）、没食子酸甲酯（4）、槲皮素（5）、槲皮素－3－0－β－D－葡萄糖甙（6）、花旗松素（7）、洋芹素（8）和蛇葡萄素（9）。9个化合物均为首次从该植物中分离得到。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.