柿子滩遗址第9地点出土的动物烧骨

柿子滩遗址第9地点（S9）位于山西省吉县柏山寺乡高楼河村黄河支流的清水河畔,西距黄河约7 km。从2000年发现至今,该遗址前后历经三次发掘,出土大量细石叶制品、动物化石、数件蚌制品、骨针及磨制石器等。本文重点对S9地点第4 层（12,575-11,600 cal. BP）及第5层（13,000 cal. BP）出土的动物遗存,尤其是其中测量尺寸在2cm以下的大量烧骨进行了埋藏学与动物考古学方面的观察和分析。研究结果显示,S9地点的烧骨是古人类烧烤猎物、维护遗址（甚至可能还包括以骨骼作燃料）等生存行为活动的文化残留。此外,S9地点出土烧骨的空间分布分析表明,古人类在上述行为活动之后,可能又将烧灼后的残存骨骼（与灰烬等）清理出火塘并堆放在其核心生活区的周边位置。     

旧石器遗址出土烧骨的技术分析及其考古学应用

烧骨常见于各类旧石器时代遗址中,作为用火残留物的一类,烧骨对研究人类用火行为有着极为重要的意义,而目前国内针对烧骨的研究工作开展得尚不够深入。本文通过介绍目前烧骨研究领域中若干种主流的分析手段及其在考古学上的应用,以求能进一步完善国内关于旧石器时代人工用火的研究框架,同时希望能对中国古人类用火能力的相关研究提供有益的借鉴。     

旧石器遗址出土烧骨的技术分析及其对考古学的启示

烧骨常见于各类旧石器时代遗址中,作为用火残留物的一类,烧骨对研究人类用火行为有着极为重要的意义,而目前国内针对烧骨的研究工作开展得尚不够深入。本文通过介绍目前烧骨研究领域中若干种主流的分析手段及其在考古学上的应用,以求能进一步完善国内关于旧石器时代人工用火的研究框架,同时希望能对中国古人类用火能力的相关研究提供有益的借鉴。     

《日本动物考古学基础》评介

动物考古学(Zooarchaeology)通过对人类各个历史时期考古遗址中发掘出的动物骨骼的鉴定与解释,了解古代居民居址附近的自然条件和生态环境、古人类狩猎的对象、对食物的选择以及家畜蓄养等情况,也采用动物学方法研究考古学相关问题.1976年,在法国举行的第九届史前学与原史学国际会议上,正式成立了国际动物考古学会( International Council for Zooarchaeology).至此,其逐渐成为考古学的一个重要分支.     

蔚县盆地吉家庄旧石器遗址动物骨骼的埋藏学分析

泥河湾盆地南部蔚县盆地区域的河湖相地层发育良好,并保存有丰富的考古材料与人类活动信息,在研究我国北方中更新世人类演化与行为方面有重要的价值。本文对蔚县盆地吉家庄遗址群4个地点出土的动物遗存进行了初步埋藏学观察与研究。结果显示,A与D地点的动物骨骼数量较少,反映的埋藏信息有限,不作为本文主要讨论对象;E地点出土的部分动物骨骼带有异地埋藏的特点,人类行为信息可能受到干扰,变得模糊;B地点动物化石接近原地埋藏,中更新世古人类应该是该地点动物资源的初级利用者与动物骨骼富集的主要动因。分析表明,B地点古人类于原地对大中型食草动物进行了肢解、割肉与敲骨取髓等多种活动,随后食肉动物又利用了这些动物骨骼的剩余营养成分,但动物骨骼数量与组成说明古人类在此地点的生存活动是短暂且有限的。     

古人类骨中羟磷灰石的XRD和喇曼光谱分析

人骨残骸是生物考古的主要对象,而骨骼污染鉴别是样品选择的依据,也是生物考古的前提。利用X射线衍射（XRD）和喇曼光谱相结合的方法,通过对新疆克雅河圆沙古城遗传出土的人类骨骼中羟磷灰石的分析,来辨析骨骼污染程度。研究结果表明,两种方法的有机结合,准确反映了骨骼中羟磷石结晶度的变化,从而可简单、较为有效地鉴别古代人类骨骼样品的污染。     

宁夏鸽子山遗址第10地点出土动物骨骼的埋藏学初步观察

宁夏鸽子山遗址第10地点(QG10)位于青铜峡市西北约20km的贺兰山南麓。2014-2017年,中国科学院古脊椎动物与古人类研究所与宁夏文物考古研究所联合组队对该遗址进行了发掘,出土大量动物化石、近万件石制品以及装饰品、骨制品、结构性火塘等。本项研究基于遗址第4层出土动物化石的埋藏学初步观察认为:晚更新世末期的古人类是遗址中动物骨骼的富集者和改造者;QG10的狩猎-采集人群采取狩猎而非主动食腐的方式获取了遗址附近的大中型食草类动物,并将其完整搬运至遗址内进行后续的肢解、食肉与敲骨取髓等营养性处理过程。相对于上述动物种类而言,遗址内的小型动物则是在其营养物质之外,古人类还利用了它们的骨骼材料以制作器型规整的骨角类工具。     

贵州马鞍山遗址发现旧石器时代骨角制品

正最近一期的国际学术期刊《考古科学》(Journal of Archaeological Science)发表了中国科学院古脊椎动物与古人类研究所高星研究员所带领的的研究团队与法国波尔多大学Francesco d'Errico教授以及南非金山大学Lucinda R.Backwell等学者对贵州马鞍山遗址中17件骨角器的研究成果。骨角器是旧石器时代考古遗存中较为常见的一种工具类型。早期的骨角制品大多是古人类经简单打击修理或直接使用破裂的动物骨骼或鹿角等有机质材料的结果;而相对晚近期的同类产品则是古人类适应于骨、角等有机质材料的特定属性,通过刮削、磨制、抛光等技术手     

周口店第一地点西剖面2009-2010年发掘报告

周口店第一地点再次发掘的主要目的在于提升遗址西剖面的保护状态与水平,同时也为东亚直立人演化方面某些焦点问题的研究与解决提供机遇与可能。2009-2010年,在西剖面最顶部堆积(即第三层)的野外发掘中共计出土大中型动物化石材料约800件。埋藏学研究表明,西剖面第三层中葛氏斑鹿(Cervus(Sika)grayi)等动物属种的骨骼富集并非古人类生存行为的结果;石制品以及古人类化石材料的缺失也在一定程度上印证了这一结论。结合发掘过程中揭露出来的一系列洞穴坍塌事件的证据,我们初步认为,至少在第三层堆积形成时期,第一地点西剖面位置处的洞穴形态并不适合于古人类的栖居活动;葛氏斑鹿等骨骼材料则是当时在遗址附近活动的各种动物因意外因素陷落于其中而得以聚集的。     

生物力学测试中小梁骨试件的保存和制备方法

骨骼在平时发挥的是支撑和运动作用,在受到撞击后保护脏器的同时自身也会断裂、受损,这都是骨与力之间相互作用产生的结果。如果可以知道骨的力学属性,并带入有限元模型计算,就可以个体化地预测其生活中的各种动作是否会导致急慢性创伤、协助判断暴力事件(如交通事故、坠落等)对其骨骼造成的破坏、在术前对骨骼质量给予指导性的评估[1]等。但是人类的骨骼看似简单,却千变万化,想要确切了解其各项属性的内在联系很困难。骨骼并不是几种材料的简单堆积,如骨骼整体的强度要大于其所有部位累计的强度[2]就是一个证明。一开始,骨的测试方法遵循普通材料的测试方法,随着研究的深入,对于骨的各个部位、各个类型的属性又有了很多新的探索。寻求最佳的试件保存、制作方法是后期测试获得成功的前提,试件保存的温度、水合状态、取材方向、形状和大小都会引起误差,多因素干扰也使分析结果变得更加困难。也正是因为如此,不同学者测得的数据差异往往比较明显。本研究旨在提炼、归纳一些通用、成熟、合理的方法,为后来的研究者在实验技术方面提供参考,让研究结果更有比较意义。     

The Identification of Proteoglycans and Glycosaminoglycans in Archaeological Human Bones and Teeth

Bone tissue is mineralized dense connective tissue consisting mainly of a mineral component (hydroxyapatite) and an organic matrix comprised of collagens, non-collagenous proteins and proteoglycans (PGs). Extracellular matrix proteins and PGs bind tightly to hydroxyapatite which would protect these molecules from the destructive effects of temperature and chemical agents after death. DNA and proteins have been successfully extracted from archaeological skeletons from which valuable information has been obtained; however, to date neither PGs nor glycosaminoglycan (GAG) chains have been studied in archaeological skeletons. PGs and GAGs play a major role in bone morphogenesis, homeostasis and degenerative bone disease. The ability to isolate and characterize PG and GAG content from archaeological skeletons would unveil valuable paleontological information. We therefore optimized methods for the extraction of both PGs and GAGs from archaeological human skeletons. PGs and GAGs were successfully extracted from both archaeological human bones and teeth, and characterized by their electrophoretic mobility in agarose gel, degradation by specific enzymes and HPLC. The GAG populations isolated were chondroitin sulfate (CS) and hyaluronic acid (HA). In addition, a CSPG was detected. The localization of CS, HA, three small leucine rich PGs (biglycan, decorin and fibromodulin) and glypican was analyzed in archaeological human bone slices. Staining patterns were different for juvenile and adult bones, whilst adolescent bones had a similar staining pattern to adult bones. The finding that significant quantities of PGs and GAGs persist in archaeological bones and teeth opens novel venues for the field of Paleontology.     

0．23T稳恒磁场对不同温度离体过氧化氢酶的磁效应研究

研究了 0 .2 3T稳恒磁场对不同温度下的离体牛肝过氧化氢酶 (CAT)构象及活力的影响 ,并从分子水平讨论了磁场对不同温度的过氧化氢酶产生不同生物学效应的可能机制。将不同温度的天然酶液置于磁感应强度为0 .2 3T的磁场中分别处理一定的时间 ,处理过程中保持环境温度与酶液温度一致 ,撤离磁场后立即在相同实验条件下对其进行光谱分析及量热分析 ,并用Beers&Sizers法 (改良型 )测定酶活力。结果表明 ,磁场使 2 5℃过氧化氢酶的构象发生明显变化 ,表现为荧光偏振度增加、出现明显的差示扫描量热曲线、产生λ2 10nm～ 310nm的紫外差光谱以及λ330nm荧光发射峰的荧光强度改变 (荧光发射峰的峰位未移动 ) ,构象变化的同时酶活力增加 ;15℃过氧化氢酶的构象及活力变化规律与 2 5℃过氧化氢酶类似 ,但强度均弱于 2 5℃酶 ;而 4℃过氧化氢酶的构象及活力没有发生变化 ,表现出未受磁场处理的影响。相同实验条件下 ,磁场对不同温度的酶分子影响不同 ,随温度的增加 ,影响效应趋于显著。由于不同温度的酶分子之间的差异在于构象状态的不同 ,这表明酶分子自身的构象状态对磁场处理效果有极其重要的影响。不同温度的过氧化氢酶磁效应差异显著可能是由磁致酶构象变化的特殊机制所引起。磁场对酶分子构象的影响可能是通     

An investigation into the internal and external variables acting on crystallinity index using Fourier Transform Infrared Spectroscopy on unaltered and burned bone

The Crystallinity Index is a measure of structural order in bone and is potentially an extremely useful tool in bioarchaeology since it can assist in sequencing bones into chronological and/or stratigraphic order, act as an indicator for preservation, and assist in reconstructing burning events. Unfortunately, concerns over influencing variables and bone sampling issues inhibit full and wide-spread adoption of this approach. An attempt has been made to determine the influence of a number of internal and external variables on bone crystallinity changes. CI, C/P and C/C were calculated using Fourier Transform Infrared Spectroscopy – Attenuated Total Reflectance (FTIR-ATR) on modern burned and unburned faunal bone. Samples were taken systematically across bone elements and on the internal and external surfaces. The CI results support previous work and show increases with burning intensity. It is also shown that extrinsic variables (such as temperature and duration of burning) have more of a significant influence on CI values than intrinsic variables (such as location of sample site on the bone). A sampling strategy is suggested for those wishing to use the CI on bone in archaeological contexts.     

Molecular genetic analysis of ancient cattle bones excavated from archaeological sites in Jeju, Korea

Ancient cattle bones were excavated from archaeological sites in Jeju, Korea. We used molecular genetic techniques to identify the species and establish its relationship to extant cattle breeds. Ancient DNA was extracted from four sources: a humerus (Gonae site, A.D. 700-800), two fragments of radius, and a tooth (Kwakji site, A.D. 0-900). The mitochondrial DNA (mtDNA) D-loop regions were cloned, sequenced, and compared with previously reported sequences of various cattle breeds (9 Asian, 8 European, and 3 African). The results revealed that these bones were of the breed, Bos taurus, and a phylogenetic tree indicated that the four cattle bones formed a monophyletic group with Jeju native black cattle. However, the patterns of sequence variation and reports from archaeological sites suggest that a few wild cattle, with a different maternal lineage, may have existed on Jeju Island. Our results will contribute to further studies of the origin of Jeju native cattle and the possible existence of local wild cattle.     

The use (and misuse) of archaeological salmon data to infer historical abundance in North America with a focus on New England

Information about historical animal or plant abundance often either explicitly or implicitly informs current conservation practice. If it can be shown that an organism was not historically abundant in a region, its conservation importance may be downgraded. In contrast to abundant archaeological support for historic importance of salmon in the Pacific Northwest, historic abundance of Atlantic salmon in New England has been called into question based on the rarity of salmon bones in archaeological sites. These data have been used to argue that the importance of salmon to the region has been exaggerated and that expensive restoration efforts in some rivers should be reconsidered. Here, we argue that lack of archaeological bone fragment abundance does not make a convincing case against historical Atlantic salmon abundance in New England for three primary reasons. First, salmon bones were rare or absent at sites that still have large salmon runs. Second, the lack of salmonid bones in general at archaeological sites suggests poor preservation and/or recovery of bone for these species relative to bones of other fishes. Third, given the presence of large numbers of non-salmonid anadromous fish in the site areas where people fished and deposited fish bones, power to detect salmon bones in studies to date may have been generally low. We present reliable historical accounts that help build a convincing case that salmon were historically abundant in New England rivers. We suggest that rarity of salmon bones in the existing archaeological data should not have unwarranted influence on present-day conservation decision-making in New England.     

角页岩雕刻器的微痕实验研究

本文以丁村遗址角页岩为原料打制实验标本,设计展开雕刻器微痕实验,并对其中部分标本按照序贯试验原理进行分阶段实验。实验结果表明,角页岩雕刻器雕刻刃的使用微痕基本特征为:轻度到重度磨圆,片疤数量较少,多小中型片疤,终端形态多为羽状和阶梯状,以分散分布为主,大片疤常单独出现。分阶段实验结果表明,磨圆是以从快到慢速度生成的,片疤在不同阶段发生改变。本文旨在通过对角页岩雕刻器使用微痕的研究,为探讨考古标本功能提供可参照的数据与图像。     

A method for estimating age of Danish medieval sub-adults based on long bone length

The preferred method for aging archaeological sub-adult skeletons is by dental examination. In cases where no dental records are available, age estimation may be performed according to epiphyseal union, skeletal elements or diaphyseal lengths. Currently no data have been produced specifically for aging archaeological Danish sub-adults from the medieval period based on diaphyseal lengths. The problem with using data on Danish samples, which have been derived from a different population, is the possibility of skewing age estimates. In this study 58 Danish archaeological sub-adults were examined, aged from approximately six years to twenty-one years. The samples were aged according to two dental methods: Haavikko and Ubelaker. Regression formulae were constructed for aging according to their diaphyseal lengths both for individual long bones and combinations of upper and lower long bones. This study indicated that with the regression formulae developed, estimation of age can be done with reasonable results on Danish sub-adults. The Danish data were then compared to data from a different archaeological sample and a modern sample. It showed that the modern data indicated a consistently lower age compared to this sample which increased until reaching a maximum of nearly five years and six months. When comparing the archaeological data to this study, the growth profile crossed over at 12.5 years with a maximum age difference before the cross point of two years and three months lower for the archaeological data. After the cross point there was a maximum difference of three years and four months higher for the archaeological data. This study has shown the importance of using data for age estimation for archaeological material which has been developed specifically for that population. In addition it has presented a possible solution for Danish sub-adult material when dental material is not available.     

Temperature dependence on the delayed fluorescence of chlorophyll a in blue-green algae.

1. The delayed fluorescence of chlorophyll a was measured with a phosphoroscope by changing the temperature in a range of room temperatures in intact cells of blue-green algae, Anacystis nidulans, two strains of Anabaena variabilis and Plectonema boryanum, and other kinds of algae, Cyanidium caldarium and Chlorella pyrenoidosa. The induction of delayed fluorescence remarkably depended on the temperature of measurment. Nevertheless, the induction pattern was characterized by three levels of intensity; the initial rise level at the onset of excitation light, the maximum level after a period of excitation and the steady-state level after 10 min of excitation. 2. In A. nidulans and a strain of A. variabilis grown at various temperatures, close relationship was found between the phase transition of membrane lipids and the initial rise and the steady-state levels of delayed fluorescence. The initial rise level showed the maximum at the temperature of phase transition between the liquid crystalline and the mixed solid-liquid crystalline states, The steady-state levels showed a remarkable change from a high in the liquid crystalline state to a low level in the mixed solid-liquid crystalline state. 3. The millisecond decay kinetics of the delayed fluorescence measured at the steady-state level in A. nidulans grown at 38 degrees C consisted of two components with different decay rates. The half-decay time of the fast component was about 0.17 ms and was constant throughout the temperature range of measurement. The half decay time of slow component ranged from 0.6 to 1.5 ms, depending on the temperature of measurment.     

Inter‐ and intraspecific variation in the surface pattern of the dermal bones of two sturgeon species

Archaeological bone remains of sturgeon (Acipenser sturio/Acipenser oxyrinchus) from northwestern Europe are often identified to species on the basis of their surface morphology and then used to reconstruct the spatial distribution of the two species through time. The dermal bones of A. sturio are said to have an exterior surface pattern consisting of tubercles, while those of A. oxyrinchus are said to display alveoli. In the present paper, the validity of the surface pattern as a species‐specific characteristic is critically assessed. To this purpose, dermal plates from modern, genetically identified museum specimens were studied and the surface morphology observed in a series of archaeological remains was compared with the genetic identifications obtained on these same remains. The analyses show that the surface pattern of dermal bones is related to the size of the individual, with the pattern of small A. oxyrinchus being similar to that of A. sturio. In addition, variations in the surface pattern among the bones of a single individual and within the same bone have been observed. These findings explain previous conflicting results between morphological and genetic identifications and allow the formulation of some recommendations for more accurate morphological identification of isolated archaeological sturgeon dermal bones.     

Skeletal manifestations of infantile scurvy

Recent investigations of human skeletal material from the historic St. Martin's cemetery, England, found a range of abnormal lesions in six infants that are almost certainly related to scurvy. Porous and proliferative bone lesions affecting the cranial bones and scapulae were found, and this paper presents images obtained using both macroscopic and scanning electron microscope examination of the lesions. Previous work on infantile scurvy (Ortner et al., 1997-2001) relied heavily on changes at the sphenoid, which is often missing in archaeological bone, so the identification of changes attributable to scurvy on other cranial bones and the scapulae is encouraging. The ability to recognize changes related to scurvy on a range of bones will ensure an enhanced potential for recognition of this disease in future research involving archaeological bone. Research on historical documents from Birmingham dating to the eighteenth and nineteenth centuries, combined with the probable cases of scurvy identified, supports the view that the paucity of cases of infantile scurvy from the archaeological record reflects a lack of understanding and recognition of bone manifestations, rather than a lack of occurrence in this period. Changes linked to scurvy were only found in infants from the poorer sections of the community from St. Martin's, and this is almost certainly linked to patterns of food consumption and may be related to shortages of potatoes, due to blight, experienced during this period.