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1.
Currently the public interest in biosafety issues has focussed on the discussions surrounding the use of genetically modified organisms, very specifically on the use of transgenic plants in agriculture. Although many of the questions raised in connection with genetically modified organisms are of legitimate scientific interest, attention should be drawn back to a number of other more classical biosafety research areas, namely the problem of control of new and reemerging infectious diseases, the need for new vaccines, control of transport and routes of dissemination, biosafety information exchange and networking, where research results are dearly needed. In the area of modern biotechnology new applications such as gene therapy and transgenic animals will be on the list of future priorities for biosafety related activities and research. 相似文献
2.
中国是水稻的起源中心之一,分布着丰富的野生种质资源.自转基因水稻获得安全证书以来,转基因水稻与其近缘野生种间的基因流受到广泛关注.本文对转基因水稻基因流的发生及其可能引起的生态学后果进行了综述和展望.认为转基因水稻能够与栽培稻、野生稻、杂草稻、稗草等成功杂交,但基因流发生频率较低且变化较大.基因流成功发生后,由于转基因水稻具转基因新性状而有适合度优势,转基因可能只通过少数几代就进入野生种群.当转基因植株进入野生种群并在自然条件下长期存在时,转基因植株与近缘野生种间的竞争关系和相对适合度将决定混合种群的动态变化.研究转基因水稻基因流的影响及其长期生态学后果对合理保护与利用野生种质资源具有重要意义. 相似文献
3.
Do transgenic plants affect rhizobacteria populations? 总被引:1,自引:0,他引:1
Martin Filion 《Microbial biotechnology》2008,1(6):463-475
Plant genetic manipulation has led to the development of genetically modified plants (GMPs) expressing various traits. Since their first commercial use in 1996, GMPs have been increasingly used, reaching a global cultivating production area of 114.3 million hectares in 2007. The rapid development of agricultural biotechnology and release of GMPs have provided many agronomic and economic benefits, but has also raised concerns over the potential impact these plants might have on the environment. Among these environmental concerns, the unintentional impact that GMPs might have on soil‐associated microbes, especially rhizosphere‐inhabiting bacteria or rhizobacteria, represents one of the least studied and understood areas. As rhizobacteria are responsible for numerous key functions including nutrient cycling and decomposition, they have been defined as good indicator organisms to assess the general impact that GMPs might have on the soil environment. This minireview summarizes the results of various experiments that have been conducted to date on the impact of GMPs on rhizobacteria. Both biological and technical parameters are discussed and an attempt is made to determine if specific rhizobacterial responses exist for the different categories of GMPs developed to date. 相似文献
4.
Kiran K. Sharma H. C. Sharma N. Seetharama Rodomiro Ortiz 《In vitro cellular & developmental biology. Plant》2002,38(2):106-115
Summary Recombinant DNA technology has great potential to enhance and extend the advantages of conventional plant breeding, and increase
the production and productivity of crops to meet the increasing demand for food and food products in the future. Judicious
application of this technology provides opportunities for alleviating some of the major constraints to crop productivity under
subsistence farming conditions in the developing countries. Considerable progress has been made in developing strategies for
the production and deployment of transgenic crops. However, biosafety concerns have been raised regarding the deployment and
release of genetically engineered plants. This debate has divided the farming and consumer communities over acceptability
of genetically modified foods. There is a need for a thorough investigation regarding the fate of transgenic plants in the
environment, and their interaction with wild relatives and non-target organisms. The production and release of transgenic
plants should be based on experience and sound scientific reasoning. The regulatory requirements for deployment of transgenic
crops should be streamlined and harmonized, in order to achieve sustainable food production, poverty reduction, and environmental
protection in resource-poor countries in the semi-arid tropics. 相似文献
5.
转基因植物环境监测进展 总被引:2,自引:1,他引:1
近20年来,转基因植物的商业化应用规模越来越大,而转基因生物安全问题依然是转基因植物产业进一步发展的最主要制约因素。转基因植物在商业化应用之前虽然预先进行了风险评估,但是,包括环境监测在内的风险管理措施是确保转基因植物安全应用的必要手段。在转基因作物大规模应用近20年之后,其在靶标生物抗性、对生物多样性的影响、基因漂移、在生态系统中的长期存留等方面产生的环境风险已经渐渐显现出来,表明风险评估无法为转基因植物应用提供足够的安全保障,还必须通过开展系统而长期的环境监测,明确转基因植物在生产应用后的实际环境影响。联合国环境规划署和欧盟等已经制定了转基因植物环境监测的法规和技术指南,一些国家实施了系统的转基因植物环境监测。对转基因植物所产生的环境风险以及环境监测应包括的内容进行了综述。 相似文献
6.
7.
Recent advances in development of marker-free transgenic plants: Regulation and biosafety concern 总被引:1,自引:0,他引:1
During the efficient genetic transformation of plants with the gene of interest, some selectable marker genes are also used
in order to identify the transgenic plant cells or tissues. Usually, antibiotic- or herbicide-selective agents and their corresponding
resistance genes are used to introduce economically valuable genes into crop plants. From the biosafety authority and consumer
viewpoints, the presence of selectable marker genes in released transgenic crops may be transferred to weeds or pathogenic
microorganisms in the gastrointestinal tract or soil, making them resistant to treatment with herbicides or antibiotics, respectively.
Sexual crossing also raises the problem of transgene expression because redundancy of transgenes in the genome may trigger
homology-dependent gene silencing. The future potential of transgenic technologies for crop improvement depends greatly on
our abilities to engineer stable expression of multiple transgenic traits in a predictable fashion and to prevent the transfer
of undesirable transgenic material to non-transgenic crops and related species. Therefore, it is now essential to develop
an efficient marker-free transgenic system. These considerations underline the development of various approaches designed
to facilitate timely elimination of transgenes when their function is no longer needed. Due to the limiting number of available
selectable marker genes, in future the stacking of transgenes will be increasingly desirable. The production of marker-free
transgenic plants is now a critical requisite for their commercial deployment and also for engineering multiple and complex
trait. Here we describe the current technologies to eliminate the selectable marker genes (SMG) in order to develop marker-free
transgenic plants and also discuss the regulation and biosafety concern of genetically modified (GM) crops. 相似文献
8.
Gilissen Luud J. W. Metz Peter L. J. Stiekema Willem J. Nap Jan-Peter 《Transgenic research》1998,7(3):157-163
The -glucuronidase (GUS) gene is to date the most frequently used reporter gene in plants. Marketing of crops containing this gene requires prior evaluation of their biosafety. To aid such evaluations of the GUS gene, irrespective of the plant into which the gene has been introduced, the ecological and toxicological aspects of the gene and gene product have been examined. GUS activity is found in many bacterial species, is common in all tissues of vertebrates and is also present in organisms of various invertebrate taxa. The transgenic GUS originates from the enterobacterial species Escherichia coli that is widespread in the vertebrate intestine, and in soil and water ecosystems. Any GUS activity added to the ecosystem through genetically modified plants will be of no or minor influence. Selective advantages to genetically modified plants that posses and express the E. coli GUS transgene are unlikely. No increase of weediness of E. coli GUS expressing crop plants, or wild relatives that might have received the transgene through outcrossing, is expected. Since E. coli GUS naturally occurs ubiquitously in the digestive tract of consumers, its presence in food and feed from genetically modified plants is unlikely to cause any harm. E. coli GUS in genetically modified plants and their products can be regarded as safe for the environment and consumers 相似文献
9.
Xiang Yu Akira Kikuchi Takayoshi Shimazaki Akiyo Yamada Yoshihiro Ozeki Etsuko Matsunaga Hiroyasu Ebinuma Kazuo N. Watanabe 《Journal of plant research》2013,126(1):141-150
Increasing soil salinization of arable land has a major impact on the global ecosystem. One approach to increase the usable global forest area is to develop transgenic trees with higher tolerance to conditions of salt stress. An allene oxide cyclase homolog, mangrin, contains a core protein domain that enhances the salt tolerance of its host. We utilized this feature to develop improved salt-tolerant eucalyptus trees, by using transgenic Eucalyptus camaldulensis carrying the mangrin gene as a model. Since the Japanese government requires an environmental biosafety assessment for the surrounding biosphere, we performed experiments on trees grown in a special netted-house. This study examined the transgenic E. camaldulensis carrying the mangrin gene to assess the feasibility of using these transformants, and assessed their salt tolerance and environmental biosafety. We found that seven of 36 transgenic genotypes had significantly higher salt tolerance than non-transformants, and more importantly, that these plants had no significant impact on environmental biosafety. These results suggest that introduction of the mangrin gene may be one approach to safely enhance salt tolerance in genetically modified Eucalyptus species, and that the transformants have no apparent risks in terms of environmental biosafety. Thus, this study provides valuable information regarding the use of transgenic trees in situ. 相似文献
10.
Metagenomic‐based impact study of transgenic grapevine rootstock on its associated virome and soil bacteriome
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Jean‐Michel Hily Sandrine Demanèche Nils Poulicard Mélanie Tannières Samia Djennane Monique Beuve Emmanuelle Vigne Gérard Demangeat Véronique Komar Claude Gertz Aurélie Marmonier Caroline Hemmer Sophie Vigneron Armelle Marais Thierry Candresse Pascal Simonet Olivier Lemaire 《Plant biotechnology journal》2018,16(1):208-220
For some crops, the only possible approach to gain a specific trait requires genome modification. The development of virus‐resistant transgenic plants based on the pathogen‐derived resistance strategy has been a success story for over three decades. However, potential risks associated with the technology, such as horizontal gene transfer (HGT) of any part of the transgene to an existing gene pool, have been raised. Here, we report no evidence of any undesirable impacts of genetically modified (GM) grapevine rootstock on its biotic environment. Using state of the art metagenomics, we analysed two compartments in depth, the targeted Grapevine fanleaf virus (GFLV) populations and nontargeted root‐associated microbiota. Our results reveal no statistically significant differences in the genetic diversity of bacteria that can be linked to the GM trait. In addition, no novel virus or bacteria recombinants of biosafety concern can be associated with transgenic grapevine rootstocks cultivated in commercial vineyard soil under greenhouse conditions for over 6 years. 相似文献
11.
我国抗虫转基因杨树生态安全性研究进展 总被引:2,自引:0,他引:2
转基因树木与农作物相比, 人们更关注其长时间种植可能导致转基因扩散到周围野生近缘种。由于生长周期长, 转基因树木会增加转基因不稳定性, 对非靶标生物的影响, 靶标害虫对转基因植物产生抗性, 增加树木入侵性(杂草化), 以及由于基因漂移或基因逃逸对环境产生的负面影响或新的环境风险。过去十几年, 针对我国抗食叶害虫的两个商业化转Bt基因欧洲黑杨(Populus nigra)和转双抗虫基因741杨[P. alba× (P. davidiana + P. simonii) × P. tomentosa], 已开展了有关生态安全性方面的多项研究。本文围绕抗虫转基因树木生态安全性研究进展进行了综述。抗虫转基因杨树对节肢动物种群和群落结构产生了一定影响, 使昆虫的多样性提高, 但对土壤微生物区系未见明显影响。转基因欧洲黑杨通过花粉和种子发生的基因漂移几率很低。转基因杨树通过内生菌发生的水平转移可能会对环境造成的潜在危险也进行了评价。文章最后指出对抗虫转基因杨树农林复合生态系统开展生物安全研究的必要性。 相似文献
12.
The release of genetically modified plants is governed by regulations that aim to provide an assessment of potential impact on the environment. One of the most important components of this risk assessment is an evaluation of the probability of gene flow. In this review, we provide an overview of the current literature on gene flow from transgenic plants, providing a framework of issues for those considering the release of a transgenic plant into the environment. For some plants gene flow from transgenic crops is well documented, and this information is discussed in detail in this review. Mechanisms of gene flow vary from plant species to plant species and range from the possibility of asexual propagation, short- or long-distance pollen dispersal mediated by insects or wind and seed dispersal. Volunteer populations of transgenic plants may occur where seed is inadvertently spread during harvest or commercial distribution. If there are wild populations related to the transgenic crop then hybridization and eventually introgression in the wild may occur, as it has for herbicide resistant transgenic oilseed rape (Brassica napus). Tools to measure the amount of gene flow, experimental data measuring the distance of pollen dispersal, and experiments measuring hybridization and seed survivability are discussed in this review. The various methods that have been proposed to prevent gene flow from genetically modified plants are also described. The current “transgenic traits” in the major crops confer resistance to herbicides and certain insects. Such traits could confer a selective advantage (an increase in fitness) in wild plant populations in some circumstances, were gene flow to occur. However, there is ample evidence that gene flow from crops to related wild species occurred before the development of transgenic crops and this should be taken into account in the risk assessment process. 相似文献
13.
One usually thinks of plant biology as a non-controversial topic, but the concerns raised over the biosafety of genetically modified (GM) plants have reached disproportionate levels relative to the actual risks. While the technology of changing the genome of plants has been gradually refined and increasingly implemented, the commercialization of GM crops has exploded. Today's commercialized transgenic plants have been produced using Agrobacterium tumefaciens-mediated transformation or gene gun-mediated transformation. Recently, incremental improvements of biotechnologies, such as the use of green fluorescent protein (GFP) as a selectable marker, have been developed. Non-transformation genetic modification technologies such as chimeraplasty will be increasingly used to more precisely modify germplasm. In spite of the increasing knowledge about genetic modification of plants, concerns over ecological and food biosafety have escalated beyond scientific rationality. While several risks associated with GM crops and foods have been identified, the popular press, spurred by colorful protest groups, has left the general public with a sense of imminent danger. Reviewed here are the risks that are currently under research. Ecological biosafety research has identified potential risks associated with certain crop/transgene combinations, such as intra- and interspecific transgene flow, persistence and the consequences of transgenes in unintended hosts. Resistance management strategies for insect resistance transgenes and non-target effects of these genes have also been studied. Food biosafety research has focused on transgenic product toxicity and allergenicity. However, an estimated 3.5 x 10(12) transgenic plants have been grown in the U.S. in the past 12 years, with over two trillion being grown in 1999 and 2000 alone. These large numbers and the absence of any negative reports of compromised biosafety indicate that genetic modification by biotechnology poses no immediate or significant risks and that resulting food products from GM crops are as safe as foods from conventional varieties. We are increasingly convinced that scientists have a duty to conduct objective research and to effectively communicate the results--especially those pertaining to the relative risks and potential benefits--to scientists first and then to the public. All stakeholders in the technology need more effective dialogues to better understand risks and benefits of adopting or not adopting agricultural biotechnologies. 相似文献
14.
15.
Dong-Keun Lee Su-Hyun Park So-Yoon Seong Youn Shic Kim Harin Jung Yang Do Choi Ju-Kon Kim 《Plant biotechnology reports》2016,10(6):391-401
Plant biotechnology provides a powerful solution to boost agricultural productivity and nutritional quality. The development process of a transgenic crop includes multiple steps that consist of gene isolation for a target trait, generation of T0 transgenic crops, characterization of the transgene, evaluation of agronomic performance of transgenic crops, selection of elite transgenic lines and assessment of target trait efficacy. Here, we developed elite insect-resistant transgenic rice plants that may satisfy the standards of biosafety assessments. We made a construct with the insecticide cry1Ac gene for a target trait. A total of 310 T0 transgenic lines were generated and underwent extensive analysis. We selected four T3 lines that contain a single-copy transgene inserted into intergenic regions of the rice genome. During this process, we critically analyzed the transgenic lines with five checkpoints that include single copy of transgene, its integration into intergenic region, clean T-DNA arrangement, stability of transgene through generations and substantial equivalence of transgenic plants in agronomic traits other than insect resistance. Consequently, we obtained insect-resistant transgenic rice plants that can be used in practical agriculture. 相似文献
16.
Chaewan Lim Sunggil Kim Yeonok Choi Young-doo Park Sung Uk Kim Soon-Kee Sung 《Plant biotechnology reports》2007,1(1):19-25
To develop an efficient screening method for detection of the transgene in Chinese cabbage (Brassica rapa spp. pekinensis) utilizing Basta spray, optimal conditions for Basta application were examined in this study. Two transgenic Chinese cabbage
lines were obtained through Agrobacterium-mediated transformation and used as transgenic positive controls in the Basta screening experiment. Differential concentrations
of glufosinate-ammonium were sprayed into three different growth stages of 12 commercial Chinese cabbage cultivars. The results
showed that no plants could survive higher than 0.05% glufosinate-ammonium, and plants at the 2–3 leaf stage were most vulnerable
to glufosinate-ammonium. On the other hand, no damage was observed in the transgenic control plants. Reliability of the Basta
spray method was proven by showing perfect co-segregation of the tolerance to glufosinate-ammonium and the presence of the
bar gene in T1 segregating populations of the transgenic lines, as revealed by both PCR and Southern blot analyses. Using the developed
Basta screening method, we tried to investigate the transgene flow through pollen dispersal, but failed to detect any transgene-containing
non-transgenic Chinese cabbages whose parents had been planted adjacent to transgenic Chinese cabbages in field conditions.
However, the transgene was successfully detected using Basta spray from the non-transgenic plants bearing the transgene introduced
by hand-pollination. Since the Basta spray method developed in this study is easy to apply and economical, it will be a valuable
tool for understanding the mechanism of gene flow through pollen transfer and for establishing a biosafety test protocol for
genetically modified (GM) Chinese cabbage cultivars. 相似文献
17.
Using seed purity data to estimate an average pollen mediated gene flow from crops to wild relatives 总被引:6,自引:0,他引:6
Lavigne C Klein EK Couvet D 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(1):139-145
Gene flow from crops to wild related species has been recently under focus in risk-assessment studies of the ecological consequences
of growing transgenic crops. However, experimental studies addressing this question are usually temporally or spatially limited.
Indirect population-structure approaches can provide more global estimates of gene flow, but their assumptions appear inappropriate
in an agricultural context. In an attempt to help the committees providing advice on the release of transgenic crops, we present
a new method to estimate the quantity of genes migrating from crops to populations of related wild plants by way of pollen
dispersal. This method provides an average estimate at a landscape level. Its originality is based on the measure of the inverse
gene flow, i.e. gene flow from the wild plants to the crop. Such gene flow results in an observed level of impurities from
wild plants in crop seeds. This level of impurity is usually known by the seed producers and, in any case, its measure is
easier than a direct screen of wild populations because crop seeds are abundant and their genetic profile is known. By assuming
that wild and cultivated plants have a similar individual pollen dispersal function, we infer the level of pollen-mediated
gene flow from a crop to the surrounding wild populations from this observed level of impurity. We present an example for
sugar beet data. Results suggest that under conditions of seed production in France (isolation distance of 1,000 m) wild beets
produce high numbers of seeds fathered by cultivated plants.
Received: 5 February 2001 / Accepted: 26 March 2001 相似文献
18.
转Bt基因抗虫棉(Gossypium spp.)是目前国内释放面积最大的转基因作物,其生态风险问题从一开始就受到密切的关注.从生态风险评价的角度,分转基因棉花中Bt杀虫蛋白的时空表达及其对害虫的控制效果、Bt基因通过花粉传播而扩散的风险、害虫对Bt棉花抗性的进化风险、Bt棉花对非目标生物体影响的风险等几个方面,综述了Bt棉安全性评价的最新研究进展,为生物安全管理提供咨询意见,并提出了目前针对Bt棉亟待研究的内容.期望本文能够为推动生物安全的研究和生物技术的发展做出一定的贡献. 相似文献
19.
转基因作物生物安全:科学证据 总被引:1,自引:0,他引:1
通过对美国Web of Science数据平台的全部转基因作物生物安全SCI论文的检索,研究了有关转基因作物生物安全的科学证据。得出科学家比消费者更关心转基因技术的安全性;批准商业化生产的转基因技术经过了有史以来最为严格的生物学安全检验与检测,并建立了有史以来最为严格的监管体系;在所发表的全部9333篇转基因生物安全论文中,90%以上的论文证明转基因技术的安全性与传统非转基因作物无显著差异;而对于所有得出转基因食品不安全结论的论文的追踪研究发现,其研究结论被证明是在错误的研究材料或方法条件下得出的。 相似文献