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Blanca Escobar Elizabeth Huenupi Inés Godoy Jacques V. Wiertz 《Biotechnology letters》2000,22(3):205-209
Enargite (Cu3AsS4) was leached at 70°C by Sulfolobus BC in shake-flasks. The highest copper dissolution (52% after 550 h of leaching) was obtained with bacteria and 1 g l–1 ferric ion. In the absence of ferric ion, Sulfolobus BC catalyzes the bioleaching of enargite through a direct mechanism after adhesion onto the mineral surface. In ferric bioleaching, arsenic precipitated as ferric arsenate and arsenic remained associated to the solid residues, preventing the presence of a high dissolved arsenic concentration in the leaching solution. About 90% inhibition of bacterial growth rate and activity was observed for dissolved arsenic concentrations above 600 mg l–1 for As(III) and above 1000 mg l–1 for As(V). Arsenic-bearing copper ores and concentrates could be leached by Sulfolobus BC in the presence of ferric iron due to the favourable precipitation of arsenic ion as ferric arsenate, avoiding significant bacterial inhibition. 相似文献
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The cyanobacterium Nostoc commune (Nostocales) is an isolate from the Schirmacher Oasis Antarctica. The cyanobacterium is psychrotropic in nature; and maintained in laboratory at 25?°C temperature, in unialgal form. Here, we studied the change in protein profile of water soluble proteins from exponentially growing N. commune upon downshift from its optimum growth temperature (25?°C) to a low temperature (5?°C). Experimental set up used to analyze the proteome were- a sudden shift to low temperature (i.e., cold shock), after short- (8?days) and long-term acclimation (7?weeks) to low temperature (5?°C). Cold-shock resulted in an increase in Low molecular weight proteins (LMWPs) with clouding of diffused proteins. Further increase in the duration of incubation period (short- and long-term acclimation) caused dissociation of proteins, indicated by NaCl (50–600mM) induced dissociation of proteins. That is, high molecular weight proteins (HMWPs) dissociated into LMWPs resulting in an increased number of protein bands. This was further confirmed by addition of LMWPs (≤10KDa) resulting in re-association of proteins into HMWPs. Hence, we report that the cold-induced synthesis of LMWPs (≤10kDa) is a strategy adopted by the N. commune to survive at low temperature of Antarctica. 相似文献
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A. Mutzel U. M. Reinscheid G. Antranikian R. Müller 《Applied microbiology and biotechnology》1996,46(5-6):593-596
A phenol-degrading thermophilic bacterium, designated Bacillus sp. A2, was isolated from a water and mud sample from a hot spring in Iceland. The aerobic isolate grew optimally on phenol
at 65 °C. At 70 °C, 85% of the optimal growth rate was still observed. No growth was observed at 40 °C and 75 °C. Bacillus sp. A2 is a gram-positive spore-forming rod. According to 16S rDNA analysis Bacillus sp. A2 is closely related to Bacillus stearothermophilus, Bacillus kaustophilus and Bacillus thermoleovorans. Bacillus sp. A2 degraded phenol completely in concentrations up to 5 mM. In addition, all three isomers of cresol were utilized as
sole carbon and energy sources. The degradation of phenols proceeds via the meta-cleavage pathway and the enzymes involved in its degradation are constitutively expressed.
Received: 13 May 1996 / Received revision: 29 July 1996 / Accepted: 12 August 1996 相似文献
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Phosphoenolpyruvate carboxylase (PEPCase; EC 4.1.1.31) from Flaveria trinervia Mohr (C4), F. floridana Johnston (C3–C4), and F. cronquistii Powell (C3) leaves were compared by electrotransfer blotting/enzyme-linked immunoassay (Western-blot analysis), mobility of the native enzyme in polyacrylamide gels and in isoelectric focusing (IEF) gels, peptide mapping, and in-vitro translation of RNA isolated from each plant. The PEPCases from the C3 and C3–C4 plants were very similar to each other in terms of electrophoretic mobilities on gels and isoenzyme patterns on IEF gels, and identical in peptide mapping. Quantitative differences were noted, however, in that the C3–C4 intermediate plant contained more PEPCase overall and that the relative activity of individual isoenzymes shifted between the C3 and C3–C4 intermediate PEPCases. The PEPCase from the C4 plant had a different isoenzyme pattern, a different peptide map, and was far more abundant than the other two enzymes. Western blot analysis demonstrated the cross-reactivity of PEPCases from all three Flaveria species with antibody raised against maize PEPCase. The results provide evidence, at the molecular level, that supports the view of C3–C4 intermediate species as C3-like plants with some C4-like photosynthetic characteristics, but there are differences from the C3 plant in the quantity and properties of the PEPCase from the C3–C4 intermediate plant.Abbreviations IEF
isoelectric focusing
- kDa
kilodalton
- PEPCase
phosphoenolpyruvate carboxylase
- Rubisco
Ribulose-1,5-bisphosphate carboxylase/oxygenase 相似文献
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C3植物中C4途径的研究进展 总被引:1,自引:0,他引:1
综述了C3植物中C4途径的发现及研究现状;阐述了C3植物中C4途径的几种作用机理;根据C3植物中C4途径的存在,探讨了改造C3植物的遗传特性;并展望了这一领域的研究前景。 相似文献
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《上海生物医学工程》2012,(2):81-81
2012年5月19日,美敦力宣布722实时动态胰岛素泵系统在中国上市,这是国内首款3C整合系统的胰岛素泵。所谓3C整合系统,即同时实现实时动态血糖监测(CGM)、胰岛素持续输注(CSII)与糖尿病信息管理(CareLink)。 相似文献
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如何快速鉴别C3与C4植物 总被引:1,自引:0,他引:1
在农业实践和科学研究中经常需要知道某种植物是C_3植物还是C_4植物,例如在干旱少雨的地区种植C_4作物就易获得较高的产量;用甲醇喷洒植物能使植物增产,但这种技术只适用于C_3植物而不适用于C_4植物等。从理论上讲,C_3植物光合作用固定CO_2的最初产物是三碳的3—磷酸甘油酸,C_4植物光合作用固定CO_2的最初产物是四碳的苹果酸或天冬氨酸。我们在研究农田杂草光合碳同化途径时,摸索了一些快速区分C_3植物与C_4植物的经验,介绍如下。 从植物进化方面区分 我们知道,C_3植物较原始,C_4植物较进化,实际上较原始的蕨类植物和裸子植物就没有C_4植物,只有较进化 相似文献
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蛋白C(ProteinC,PC)系统是存在于人血浆中的天然抗凝系统,由蛋白C、蛋白S(PS)、血栓调节蛋白(TM)和蛋白C抑制剂(PCI)组成。许多研究结果表明:PC的病理改变与血栓性疾病的发生发展密切相关,PC可作为血栓性疾病诊断、疗效观察和预后评价的参考指标;PC和蛇毒PC激活剂有显著的抗凝溶栓效果,有应用于临床的可能性。现就这方面的进展作一简要综述。1 PC的生理功能和生物学特性 PC是维生素K依赖性糖蛋白,以无活性的酶原形式存在于血浆中,由肝脏合成。PC是机体的一种重要的抗凝因子,约占血液抗凝活力的20%~30%,PC在微循环中的抗凝… 相似文献
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C3、C4和C3-C4中间型植物的进化 总被引:1,自引:1,他引:0
介绍了有关C3、C4和C3-C4中间型植物进化的形态学、生理学、分子生物学、遗传学等方面的证据;推断地球上首先出现C3植物,然后是C3-C4中间类型植物,最后出现C4植物. 相似文献
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C_3和C_4植物的氮素利用效率何新华,AnnOaks,李明启(云南师范大学生物系,昆明650092)(圭尔夫大学植物系,加拿大NIG2W1)(华南农业大学农业生物系,广州510642)关键词C_3和C_4植物,NO吸收与积累,硝酸还原酶酶蛋白,硝酸... 相似文献
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Four populations of Mollugo verticillata L. were compared on the basis of their photosynthetic products, photosynthetic rates, enhancement under low oxygen concentration, and CO2 compensation points. In addition, pulse-chase labeling experiments were conducted using one of the four populations. Depending on the plant population, C4 acids ranged from 40% to 11% of the primary products under short-term exposure to 14CO2. These compounds were also metabolized during pulse-chase experiments. All four populations had significantly different photosynthetic rates and those rates were correlated with the amounts of labelled C4 acids produced and C4-acid turnover. Three populations of M. verticillata had similar compensation points (40 l/l) and degrees of photosynthetic enhancement under low [O2] (20%), the fourth population was much lower in both characteristics (CO2 compensation, 25 l/l; low-O2 enhancement, 12%). The results verify the intermediate nature of photosynthesis in this species, and illustrate populational differences in its photosynthetic and photorespiratory carbon metabolism.Abbreviations PGA
3-phosphoglyceric acid
- Kan
Kansas
- Mass
Massachusetts
- Mex
Mexico 相似文献
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C3和C4禾本科作物的氮素利用效率 总被引:11,自引:0,他引:11
C_3和C_4禾本科作物的氮素利用效率何新华安·奥克斯(云南师范大学生命科学系,昆明650092)(加拿大圭尔夫大学植物系圭尔夫NlG.ZA1)李明启(华南农业大学农业生物系广州510642)THEEFFCIENCYOFNITROGENUTILIZA... 相似文献