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1.
Toward the mapping of physiological and agronomic characters on a rice function map: QTL analysis and comparison between QTLs and expressed sequence tags 总被引:26,自引:0,他引:26
K. Ishimaru M. Yano N. Aoki K. Ono T. Hirose S. Y. Lin L. Monna T. Sasaki R. Ohsugi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(6-7):793-800
We have constructed a rice function map by collating the results on quantitative trait loci (QTLs) for 23 important physiological
and agronomic characters (including 13 newly measured traits) obtained using backcross inbred lines of japonica Nipponbare×indica Kasalath. Using these materials, The Rice Genome project (RGP) has developed a high-density genetic map. QTLs controlling
yield did not overlap with those controlling the morphological and physiological traits supposed to relate to yield, such
as photosynthetic ability. This result suggests that these traits do not influence yield, at least in this genetic background
and environment. QTLs controlling yield also did not overlap with the structural genes controlling carbon metabolism (rbcS, cytosolic or plastidic fructose-1,6-bisphosphate, R-enzyme, and sucrose synthase).The combination of a function map and results from the RGP can be advantageous. The utility of this map is discussed.
Received: 1 October 1999 / Accepted: 28 July 2000 相似文献
2.
QTL analysis of an advanced backcross of Lycopersicon peruvianum to the cultivated tomato and comparisons with QTLs found in other wild species 总被引:12,自引:0,他引:12
T. M. Fulton T. Beck-Bunn D. Emmatty Y. Eshed J. Lopez V. Petiard J. Uhlig D. Zamir S. D. Tanksley 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,95(5-6):881-894
A BC3 population previously developed from a backcross of Lycopersicon peruvianum, a wild relative of tomato, into the cultivated variety L. esculentum was analyzed for QTLs. Approximately 200 BC4 families were scored for 35 traits in four locations worldwide. One hundred and sixty-six QTLs were detected for 29 of those
traits. For more than half of those 29 traits at least 1 QTL was detected for which the presence of the wild allele was associated
with an agronomically beneficial effect despite the inferior phenotype of the wild parent. Eight QTLs for fruit weight could
be followed through the BC2, BC3, and BC4, generations, supporting the authenticity of these QTLs. Comparisons were made between the QTLs found in this study and those
found in studies involving two other wild species; the results showed that while some of these QTLs can be presumed to be
allelic, most of the QTLs detected in this study are ones not previously discovered.
Received: 9 April 1997 / Accepted: 20 May 1997 相似文献
3.
L. A. Marquez-Cedillo P. M. Hayes B. L. Jones A. Kleinhofs W. G. Legge B. G. Rossnagel K. Sato S. E. Ullrich D. M. Wesenberg North American Barley Genome Mapping Project 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,101(1-2):173-184
Characterization of the determinants of economically important phenotypes showing complex inheritance should lead to the more
effective use of genetic resources. This study was conducted to determine the number, genome location and effects of QTLs
determining malting quality in the two North American barley quality standards. Using a doubled-haploid population of 140
lines from the cross of Harrington×Morex, malting quality phenotype data sets from eight environments, and a 107-marker linkage
map, QTL analyses were performed using simple interval mapping and simplified composite interval mapping procedures. Seventeen
QTLs were associated with seven grain and malting quality traits (percentage of plump kernels, test weight, grain protein
percentage, soluble/total protein ratio, α-amylase activity, diastatic power and malt-extract percentage). QTLs for multiple
traits were coincident. The loci controlling inflorescence type [vrs1 on chromosome 2(2H) and int-c on chromosome 4(4H)] were coincident with QTLs affecting all traits except malt-extract percentage. The largest effect QTLs,
for the percentage of plump kernels, test weight protein percentage, S/T ratio and diastatic power, were coincident with the
vrs1 locus. QTL analyses were conducted separately for each sub-population (six-rowed and two-rowed). Eleven new QTLs were detected
in the subpopulations. There were significant interactions between the vrs1 and int-c loci for grain-protein percentage and S/T protein ratio. Results suggest that this mating of two different germplasm groups
caused a disruption of the balance of traits. Information on the number, position and effects of QTLs determining components
of malting quality may be useful for maintaining specific allele configurations that determine target quality profiles.
Received: 28 May 1999 / Accepted: 9 November 1999 相似文献
4.
L. A. Marquez-Cedillo P. M. Hayes A. Kleinhofs W. G. Legge B. G. Rossnagel K. Sato S. E. Ullrich D. M. Wesenberg 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(4):625-637
A better understanding of the genetics of complex traits, such as yield, may be achieved by using molecular tools. This study
was conducted to estimate the number, genome location, effect and allele phase of QTLs determining agronomic traits in the
two North American malting barley (Hordeum vulgare L.) quality variety standards. Using a doubled haploid population of 140 lines from the cross of two-rowed Harrington×six-rowed
Morex, agronomic phenotypic data sets from nine environments, and a 107-marker linkage map, we performed QTL analyses using
simple interval mapping and simplified composite interval mapping procedures. Thirty-five QTLs were associated, either across
environments or in individual environments, with five grain and agronomic traits (yield, kernel plumpness, test weight, heading
date, and plant height). Significant QTL×environment interaction was detected for all traits. These interactions resulted
from both changes in the magnitude of response and changes in the sign of the allelic effect. QTLs for multiple traits were
coincident. The vrs1 locus on chromosome 2 (2H), which determines inflorescence row type, was coincident with the largest-effect QTL determining
four traits (yield, kernel plumpness, test weight, and plant height). QTL analyses were also conducted separately for each
sub-population (six-rowed and two-rowed). Seven new QTLs were detected in the sub-populations. Positive transgressive segregants
were found for all traits, but they were more prevalent in the six-rowed sub-population.QTL analysis should be useful for
identifying candidate genes and introgressing favorable alleles between germplasm groups.
Received: 18 August 2000 / Accepted: 15 December 2000 相似文献
5.
David D Fang Johnie N Jenkins Dewayne D Deng Jack C McCarty Ping Li Jixiang Wu 《BMC genomics》2014,15(1)
Background
Upland cotton (Gossypium hirsutum L.) accounts for about 95% of world cotton production. Improving Upland cotton cultivars has been the focus of world-wide cotton breeding programs. Negative correlation between yield and fiber quality is an obstacle for cotton improvement. Random-mating provides a potential methodology to break this correlation. The suite of fiber quality traits that affect the yarn quality includes the length, strength, maturity, fineness, elongation, uniformity and color. Identification of stable fiber quantitative trait loci (QTL) in Upland cotton is essential in order to improve cotton cultivars with superior quality using marker-assisted selection (MAS) strategy.Results
Using 11 diverse Upland cotton cultivars as parents, a random-mated recombinant inbred (RI) population consisting of 550 RI lines was developed after 6 cycles of random-mating and 6 generations of self-pollination. The 550 RILs were planted in triplicates for two years in Mississippi State, MS, USA to obtain fiber quality data. After screening 15538 simple sequence repeat (SSR) markers, 2132 were polymorphic among the 11 parents. One thousand five hundred eighty-two markers covering 83% of cotton genome were used to genotype 275 RILs (Set 1). The marker-trait associations were analyzed using the software program TASSEL. At p < 0.01, 131 fiber QTLs and 37 QTL clusters were identified. These QTLs were responsible for the combined phenotypic variance ranging from 62.3% for short fiber content to 82.8% for elongation. The other 275 RILs (Set 2) were analyzed using a subset of 270 SSR markers, and the QTLs were confirmed. Two major QTL clusters were observed on chromosomes 7 and 16. Comparison of these 131 QTLs with the previously published QTLs indicated that 77 were identified before, and 54 appeared novel.Conclusions
The 11 parents used in this study represent a diverse genetic pool of the US cultivated cotton, and 10 of them were elite commercial cultivars. The fiber QTLs, especially QTL clusters reported herein can be readily implemented in a cotton breeding program to improve fiber quality via MAS strategy. The consensus QTL regions warrant further investigation to better understand the genetics and molecular mechanisms underlying fiber development.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-397) contains supplementary material, which is available to authorized users. 相似文献6.
QTL mapping of fruit-related traits in pepper (Capsicum annuum) 总被引:11,自引:0,他引:11
A. Ben Chaim I. Paran R. C. Grube M. Jahn R. van Wijk J. Peleman 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(6-7):1016-1028
QTL analysis of pepper fruit characters was performed in an F3 population derived from a cross between two Capsicum annuum genotypes, the bell-type cultivar Maor and the Indian small-fruited line Perennial. RFLP, AFLP®1, RAPD and morphological markers (a total of 177) were used to construct a comparative pepper-tomato genetic map for this cross, and 14 quantitatively inherited traits were evaluated in 180 F3 families. A total of 55 QTL were identified by interval analysis using LOD 3.0 as the threshold for QTL detection. QTL for several traits including fruit diameter and weight, pericarp thickness and pedicel diameter were often located in similar chromosomal regions, thus reflecting high genetic correlations among these traits. A major QTL that accounts for more than 60% of the phenotypic variation for fruit shape (ratio of fruit length to fruit diameter) was detected in chromosome 3. This chromosome also contained QTL for most of the traits scored in the population. Markers in linkage groups 2, 3, 8 and 10 were associated with QTL for multiple traits, thereby suggesting their importance as loci that control developmental processes in pepper. Several QTL in pepper appeared to correspond to positions in tomato for loci controlling the same traits, suggesting the hypothesis that these QTL may be orthologous in the two species. 相似文献
7.
S. Kumar R. J. Spelman D. J. Garrick T. E. Richardson M. Lausberg P. L. Wilcox 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(6):926-933
The objective of this study was to determine the genetic location and effects of genomic regions controlling wood density
at three stages, i.e., rings corresponding to ages 1–5 (WD1_5), rings corresponding to ages 6–10 (WD6_10), and outer wood
density (WD14) in a full-sib pedigree (850.055×850.096) of Pinus radiata. The number of offspring measured at these three stages were 80, 93 and 93, respectively. Only a single linkage group of
the parent 850.55 was considered for mapping quantitative trait loci (QTLs). A multiple-marker least-squares approach was
employed for mapping QTLs for each of the three traits, using a single-QTL model. Logistic regression was used for multiple-trait
QTL mapping. Critical values for test-statistic were calculated empirically by ’shuffling’ the data. A putative QTL with large
effect on WD1_5 appears to be segregating at the 73 cM position (experimentwise P<0.01). The width of the 95% bootstrap confidence interval for this putative QTL was 40 cM (i.e. 56–96 cM). The effect of
this QTL on the expression of wood density at later stages was diminished. From multiple-trait analysis, two marker locations
(at 66 cM and 91 cM) were found to be significantly associated (experimentwise P <0.05) with the expression of wood density at different ages. These results are encouraging for the application of marker
information to early selection in order to increase juvenile wood density, although the putative QTLs detected in this study
need to be verified in an independent population.
Received: 23 July 1999 / Accepted: 27 August 1999 相似文献
8.
Identification and estimation of a QTL model and its effects 总被引:47,自引:0,他引:47
J.-Y. Gai J.-K. Wang 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(7):1162-1168
A joint segregation analysis of a genetic system and the effects of QTLs based on the six populations P1, F1, P2, B1, B2 and F2 is proposed in this paper. The major steps were as follows. Firstly, under the supposition that the segregating population
was composed of component distributions controlled by a major gene(s) and modified by both polygenes and environments, four
groups and 17 types of genetic models, including a one major-gene model, a two major-gene model, a polygene model, and a mixed
one-major gene and polygene model, were set up. Secondly, the joint maximum-likelihood function was constructed from the six
generations so as to estimate the parameters of component distributions through an EM algorithm. Thirdly, the best-fitting
genetic model was chosen according to Akaike’s information criterion, a likelihood-ratio test, and tests for goodness of fit.
Fourthly, the related genetic parameters, including gene effects, as well as the genetic variances of major genes and polygenes,
were obtained from the estimates of component distributions. Finally, the individuals in segregating populations were classified
into their major-gene genotypes according to their posterior probabilities. An example of the genetic analysis of plant height
of a rice cross between Nanjing No. 6 and Guangcong was used to illustrate the above procedure. The method was especially
appropriate to those crops with easy to obtain hybrid seeds.
Received: 11 February 1998 / Accepted: 28 May 1998 相似文献
9.
H. El Attari P. M. Hayes A. Rebai G. Barrault G. Dechamp-Guillaume A. Sarrafi 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):95-100
Genetic variability for partial resistance to bacterial leaf streak in barley, caused by Xanthomonas campestris pv. hordei, was investigated in 119 doubled-haploid lines (DH) developed by the Hordeum bulbosum method from the F1 progeny of the cross between two cultivars, ‘Morex’ (resistant) and ‘Steptoe’ (susceptible). Two experiments were undertaken
in a randomized complete block design with three replicates, in a controlled growth chamber. Twenty seeds per replicate were
planted in plastic containers (60×40×8 cm) containing moistened vermiculite. At the two-leaf stage seedlings were inoculated
with an Iranian strain of the pathogen. Genetic variability was observed among the 119 DH lines for partial resistance to
the disease. Some DH lines were significantly more resistant than ‘Morex’ (resistant parent) to bacterial leaf streak. Genetic
gain in percentage of resistant parent for 5% of the selected DH lines was significant (47.70% and 33.72% in the first and
the second experiment, respectively). A QTL analysis of bacterial leaf streak resistance showed that three QTLs were detected
on chromosomes 3 and 7. Multilocus allelic effects of the three QTLs account for almost 54% of the mean difference between
the parents and nearly 30% of the phenotypic variation of the trait in the mean experiment. The resistance locus on chromosome
3, near ABG377, apprears to be a major gene.
Received: 15 July 1997 / Accepted: 4 August 1997 相似文献
10.
Pollen thermotolerance is an important component of the adaptability of crops to high temperature stress. The tolerance level of the different genotypes in a population of 45 maize recombinant inbred lines was determined as the degree of injury caused by high temperature to pollen germinability (IPGG) and pollen tube growth (IPTG) in an in vitro assay. Both traits revealed quantitative variability and high heritability. The traits were genetically dissected by the analysis of molecular markers using 184 mapped restriction fragment length polymorphisms (RFLPs). Significant genetic correlation between the markers and the trait allowed us to identify a minimum number of five quatitative trait loci (QTLs) for IPGG and six QTLs for IPTG. Their chromosomal localization indicated that the two characters are controlled by different sets of genes. In addition, IPGG and IPTG were shown to be basically independent of the pollen germination ability and pollen tube growth rate under non-stress conditions. These results are discussed in relation to their possible utilization in a breeding strategy for the improvement of thermotolerance in maize. 相似文献
11.
V. Jorge M. Fregene C. M. Vélez M. C. Duque J. Tohme V. Verdier 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(4):564-571
We evaluated cassava bacterial blight (CBB) infection in an pair-cross population of 150 individuals derived from an intra-specific
cross between two non-inbred cassava (Manihot esculenta Crantz) lines. The replicated trials were carried out in the field under high disease pressure over two consecutive crop
cycles. Evaluations were conducted at 4 and 7 months after planting for the two cycles. Simple regression analysis and the
nonparametric Kruskal-Wallis rank-sum test revealed that eight quantitative trait loci (QTLs) were involved in resistance.
We detected changes in QTLs from crop cycle to crop cycle. The pathogen population (Xanthomonas axonopodis pv. manihotis) was also monitored over the period, using a restriction fragment length polymorphism probe and pathogenic tests. Changes
in QTL detection over the 2 years could be correlated with changes in pathogen population structure. One QTL, located in linkage
group D, was conserved over the two crop cycles, and in field to greenhouse evaluations. This study thus identified molecular
markers useful for marker assisted-selection, a technique that can accelerate the long, multiple-season process of breeding
for CBB resistance.
Received: 1 January 2000 / Accepted: 25 June 2000 相似文献
12.
E. Dirlewanger A. Moing C. Rothan L. Svanella V. Pronier A. Guye C. Plomion R. Monet 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(1):18-31
The organoleptic quality of fleshy fruits is in a large part defined by their composition of soluble sugars and organic acids.
An F2 population issuing from a cross between two peach varieties, ‘Ferjalou Jalousia’, a non-acid peach, and ‘Fantasia’, an acid
nectarine, was analysed over 2 successive years for agronomic characters and for molecular-marker (isoenzymes, RFLPs, RAPDs,
IMAs and AFLPs) segregations. Blooming and maturity dates, as well as productivity, were noted for each tree. Four fruits
per tree were analysed at maturity for fresh weight, colour, pH, titratable acidity, soluble-solids content (SSC), acid (malic,
citric and quinic acids) and sugar (sucrose, glucose, fructose, sorbitol) contents. QTLs were detected for all fruit components
analysed, except for fruit colour. The QTLs for nearly all components were present on two linkage groups. For productivity,
fresh weight, pH, quinic acid, sucrose and sorbitol content, all the detected QTLs displayed the same effect as the parental
phenotypes. By contrast, for maturity date, titratable acidity, malic and citric acids and fructose, some QTLs displayed the
same effect as the parental phenotypes while others displayed the opposite effect. The fraction of the total variation in
each trait throughout the population explained by the QTLs was very high and reached more than 90% for some characters. For
most of the characters analysed, epistasis was observed between QTLs.
Received: 10 October 1997 / Accepted: 18 August 1998 相似文献
13.
AFLP mapping of quantitative trait loci for yield-determining physiological characters in spring barley 总被引:15,自引:0,他引:15
X. Yin P. Stam C. Johan Dourleijn M. J. Kropff 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(1-2):244-253
An amplified fragment length polymorphism (AFLP) map covering 965 cM was constructed using 94 recombinant inbred lines of
a cross between the spring barley varieties Prisma and Apex. This map was employed to identify quantitative trait loci (QTLs)
controlling plant height, yield and yield-determining physiological characters using an approximate multiple-QTL model, the
MQM method. The seven physiological traits were parameters used in a process-based crop-growth model that predicts barley
biomass production as affected by daily temperature and radiation. The traits were measured in experiments conducted over
2 years. Except for the relative growth rate of leaf area, all traits examined had at least one QTL in each year. QTLs and
their effects were found to vary with developmental stages for one trait, the fraction of shoot biomass partitioned to leaves,
that was measured at several stages. Most of the traits were associated, though to different extents, with the denso dwarfing gene (the height-reducing allele in Prisma) located on the long arm of chromosome 3. Some of the QTLs were mapped
to similar positions in both years. The results in relation to effects of the dwarfing gene, the physiological basis for QTL×environment
interaction, and the relative importance of the parameter traits with respect to yield, are discussed.
Received: 17 September 1998 / Accepted: 28 December 1998 相似文献
14.
RFLP mapping of quantitative trait loci controlling abscisic acid concentration in leaves of drought-stressed maize (Zea mays L.) 总被引:7,自引:0,他引:7
R. Tuberosa M. C. Sanguineti P. Landi S. Salvi E. Casarini S. Conti 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):744-755
Abscisic acid (ABA) concentration in leaves of drought-stressed plants is a quantitatively inherited trait. In order to identify
quantitative trait loci (QTLs) controlling leaf ABA concentration (L-ABA) in maize, leaf samples were collected from 80 F3:4 families of the cross Os420 (high L-ABA)×IABO78 (low L-ABA) tested under drought conditions in field trials conducted over
2 years. In each year, leaf samples were collected at stem elongation and near anthesis. The genetic map obtained with 106
restriction fragment length polymorphism (RFLP) loci covered 1370 cM, which represented approximately 85% of the UMC maize
map. Sixteen different QTLs with a LOD>2.0 were revealed in at least one sampling. Across samplings, only four QTLs significantly
influenced L-ABA, accounting for 66% of the phenotypic variation and 76% of the genetic variation among families. At these
QTLs, the alleles which increased L-ABA were contributed by Os420. The two most important QTLs were mapped on chromosome 2
near csu133 and csu109a. The effects associated with the QTL near csu133 were more pronounced near anthesis. The support intervals of the four primary QTLs for L-ABA did not overlap the presumed
map position of mutants impaired in ABA biosynthesis.
Received: 27 January 1998 / Accepted: 22 April 1998 相似文献
15.
Background and Aims
Low phosphorus (P) availability is a major constraint to soybean growth and production, especially in tropical and subtropical areas. Root traits have been shown to play critical roles in P efficiency in crops. Identification of the quantitative trait loci (QTLs) conferring superior root systems could significantly enhance genetic improvement in soybean P efficiency.Methods
A population of 106 F9 recombinant inbred lines (RILs) derived from a cross between BD2 and BX10, which contrast in both P efficiency and root architecture, was used for mapping and QTL analysis. Twelve traits were examined in acid soils. A linkage map was constructed using 296 simple sequence repeat (SSR) markers with the Kosambi function, and the QTLs associated with these traits were detected by composite interval mapping and multiple-QTL mapping.Key Results
The first soybean genetic map based on field data from parental genotypes contrasting both in P efficiency and root architecture was constructed. Thirty-one putative QTLs were detected on five linkage groups, with corresponding contribution ratios of 9·1–31·1 %. Thirteen putative QTLs were found for root traits, five for P content, five for biomass and five for yield traits. Three clusters of QTLs associated with the traits for root and P efficiency at low P were located on the B1 linkage group close to SSR markers Satt519 and Satt519-Sat_128, and on the D2 group close to Satt458; and one cluster was on the B1 linkage group close to Satt519 at high P.Conclusions
Most root traits in soybean were conditioned by more than two minor QTLs. The region closer to Satt519 on the B1 linkage group might have great potential for future genetic improvement for soybean P efficiency through root selection. 相似文献16.
Mapping of QTL for downy mildew resistance in maize 总被引:4,自引:0,他引:4
H. A. Agrama M. E. Moussa M. E. Naser M. A. Tarek A. H. Ibrahim 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):519-523
Quantitative trait loci (QTLs) of maize involved in mediating resistance to Peronosclerospora sorghi, the causative agent of sorghum downy mildew (SDM), were detected in a population of recombinant inbred lines (RILs) derived
from the Zea mays L. cross between resistant (G62) and susceptible (G58) inbred lines. Field tests of 94 RILs were conducted over two growing
seasons using artificial inoculation. Heritability of the disease reaction was high (around 70%). The mapping population of
the RILs was also scored for restriction fragment length polymorphic (RFLP) markers. One hundred and six polymorphic RFLP
markers were assigned to ten chromosomes covering 1648 cM. Three QTLs were detected that significantly affected resistance
to SDM combined across seasons. Two of these mapped quite close together on chromosome 1, while the third one was on chromosome
9. The percentage of phenotypic variance explained by each QTL ranged from 12.4% to 23.8%. Collectively, the three QTLs identified
in this study explained 53.6% of the phenotypic variation in susceptibility to the infection. The three resistant QTLs appeared
to have additive effects. Increased susceptibility was contributed by the alleles of the susceptible parent. The detection
of more than one QTL supports the hypothesis that several qualitative and quantitative genes control resistance to P. sorghi. 相似文献
17.
Mapping of the QTL (quantitative trait locus) conferring partial resistance to leaf blast in rice cultivar Chubu 32 总被引:7,自引:0,他引:7
Zenbayashi K Ashizawa T Tani T Koizumi S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2002,104(4):547-552
The rice cultivar Chubu 32 possesses a high level of partial resistance to leaf blast. The number and chromosomal location
of genes conferring this resistance were detected by restriction fragment length polymorphism (RFLP) linkage mapping and quantitative
trait locus (QTL) analysis. For the mapping, 149 F3 lines derived from the cross between rice cultivar Norin 29, with a low level of partial resistance, and Chubu 32 were used,
and their partial resistance to leaf blast was assessed in upland nurseries. A linkage map covering six chromosomes and consisting
of 36 RFLP markers was constructed. In the map, only one significant QTL (LOD>2.0) for partial resistance was detected on
chromosome 11. This QTL explained 45.6% of the phenotypic variation. The segregation ratio of the F3 lines was 3:1 for partial
resistance to susceptibility. These results suggest that the partial resistance in Chubu 32 is controlled by a major gene.
Received: 15 March 2001 / Accepted: 13 August 2001 相似文献
18.
QTL analysis of flower and fruit traits in sour cherry 总被引:2,自引:0,他引:2
D. Wang R. Karle A. F. Iezzoni 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2000,100(3-4):535-544
The map locations and effects of quantitative trait loci (QTLs) were estimated for eight flower and fruit traits in sour cherry
(Prunus cerasus L.) using a restriction fragment length polymorphism (RFLP) genetic linkage map constructed from a double pseudo-testcross.
The mapping population consisted of 86 progeny from the cross between two sour cherry cultivars, Rheinische Schattenmorelle
(RS)×Erdi Botermo (EB). The genetic linkage maps for RS and EB were 398.2 cM and 222.2 cM, respectively, with an average interval
length of 9.8 cM. The RS/EB linkage map that was generated with shared segregating markers consisted of 17 linkage groups
covering 272.9 cM with an average interval length of 4.8 cM. Eleven putatively significant QTLs (LOD >2.4) were detected for
six characters (bloom time, ripening time, % pistil death, % pollen germination, fruit weight, and soluble solids concentration).
The percentage of phenotypic variation explained by a single QTL ranged from 12.9% to 25.9%. Of the QTLs identified for the
traits in which the two parents differed significantly, 50% had allelic effects opposite to those predicted from the parental
phenotype. Three QTLs affecting flower traits (bloom time, % pistil death, and % pollen germination) mapped to a single linkage
group, EB 1. The RFLP closest to the bloom time QTL on EB 1 was detected by a sweet cherry cDNA clone pS141 whose partial
amino acid sequence was 81% identical to that of a Japanese pear stylar RNase.
Received: 4 March 1999 / Accepted: 27 August 1999 相似文献
19.
Identification of inter simple sequence repeat (ISSR) markers associated with seed size in wheat 总被引:43,自引:0,他引:43
J. S. S. Ammiraju B. B. Dholakia D. K. Santra H. Singh M. D. Lagu S. A. Tamhankar H. S. Dhaliwal V. S. Rao V. S. Gupta P. K. Ranjekar 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(5):726-732
The feasibility of identifying inter-simple sequence repeat markers associated with seed weight in hexaploid wheat was tested
using 113 recombinant inbred lines developed by the single-seed descent method, from a cross between Rye selection111, an
Indian genetic stock obtained through the introgression of genes for bold seed size from rye, and Chinese Spring having small
seed size. Three markers were associated with low seed size with gene effects of 14.8%, 9.5%, and 6%, while four markers with
contributions of 8%, 4.66%, 2.92% and 2.61% were found to be linked to high seed size, together contributing 31% of the phenotypic
variance in seed size. Nulli-tetrasomic and di-telosomic analysis revealed the presence of three low seed size QTL-associated
markers on three chromosomes, 6BL, 2DL, and 1DS respectively. This study clearly demonstrates that ISSRs are highly useful
for finding markers associated with major and minor genes controlling agronomically important traits in wheat.
Received: 24 February 2000 / Accepted: 31 March 2000 相似文献