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Michiko Kimoto Mikako Shirouzu Shin Mizutani Hiroshi Koide Yoshito Kaziro Ichiro Hirao Shigeyuki Yokoyama 《European journal of biochemistry》2002,269(2):697-704
RNA aptamers with affinity for the Ras-binding domain (RBD) of Raf-1 were isolated from a degenerate pool by in vitro selection. These aptamers efficiently inhibited the Ras interaction with the Raf-1 RBD, and also inhibited Ras-induced Raf-1 activation in a cell-free system. The RNA aptamer with the most potent inhibitory effect specifically inhibited the Ras-Raf-1 interaction and had no affinity for the RBD of the RGL protein, a homolog of the Ral GDP dissociation stimulator. Although the aptamer was capable of binding to the B-Raf RBD, the RNA did not inhibit the interaction between Ras and the B-Raf RBD. Enzymatic and chemical probing experiments indicated that the aptamer was folded into a pseudoknot structure, and some loop regions of the pseudoknot were located at the binding interface for the Raf-1 RBD. 相似文献
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Cross-clade inhibition of recombinant human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus SIVcpz reverse transcriptases by RNA pseudoknot aptamers 下载免费PDF全文
Held DM Kissel JD Thacker SJ Michalowski D Saran D Ji J Hardy RW Rossi JJ Burke DH 《Journal of virology》2007,81(10):5375-5384
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