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1.
Bjerkandera sp. BOL 13 removed 95% of nonylphenol (at 9.7 mg nonylphenol l−1 day−1) from aqueous medium after 5 days of incubation in agitated cultures. This removal rate decreased 2.5-fold in static cultures.
By comparison, Trametes versicolor removed nonylphenol at 2.8 mg l−1 day−1 under conditions of static incubation, probably due to the action of laccase, but no growth was recorded in the agitated
bottles.
Received 4 August 2005; Revisions requested 18 August 2005 and 27 September 2005; Revisions received 22 September 2005 and
3 November 2005; Accepted 7 November 2005 相似文献
2.
The nutrition conditions needed to redirect the carbon flux in Torulopsis glabrata, a pyruvate hyper-production yeast, from pyruvate to α-ketoglutaric acid (KG) were investigated in a stirred fermentor. A minor amount of KG (1.3 gl−1) was produced when NaOH was used to control the pH, while 12 g KG l−1 was produced when CaCO3 was used instead. When thiamine and biotin were included in the medium, 13 g KG l−1 and 68 g pyruvate l−1 were produced after 48 h when glucose was nearly consumed (approximately 5 gl−1). With fermentation continuing for a further 16 h, the concentration of pyruvate decreased to 31 gl−1, and KG increased to 30 gl−1. KG thus accumulated at the expense of pyruvate consumption.
Received 2 June 2005; Revisions requested 30 June 2005 and 1 September 2005; Revisions received 1 September 2005 and 28 October
2005; Accepted 28 October 2005 相似文献
3.
A β-1,3-glucanase was detected, using laminarin as substrate, in the culture broth of Chaetomium sp. Major activity was associated with a 70 kDa protein band visualized on a polyacrylamide gel. β-1,3-Glucanase was purified
by a one-step, native gel purification procedure. Optimal activity was observed at pH 6.0 and 30 °C (over 30 min). It could
degrade cell walls of plant pathogens including Rhizoctonia solani, Gibberella zeae, Fusarium sp., Colletotrichum gloeosporioides and Phoma sp. The N-terminal amino acid residues of the purified β-1,3-glucanase are PYQLQTP, which do not exhibit homology to other fungal β-1,3-glucanases
suggesting it may be a novel enzyme.
Received 20 July 2005; Revisions requested 2 August 2005 and 27 September 2005; Revisions received 16 September 2005 and 3
November 2005; Accepted 6 November 2005 相似文献
4.
In this study, adaptive immune response was investigated in farmed southern bluefin tuna, Thunnus maccoyii, infected with a sanguinicolid Cardicola forsteri. A cohort (Cohort2005) of southern bluefin tuna was sampled between March 2005 and August 2006. Samples were taken at the transfer of wild caught tuna to sea cages and then at regular intervals. Parasite intensity, abundance and prevalence data were recorded. An ELISA was developed to detect and quantify an antibody response against the blood fluke in southern bluefin tuna serum. Intensity and prevalence of the blood fluke were shown to peak in May 2005 at 10.9 flukes per infected fish (SE = 1.72) and 97.5% prevalence and then decreased to low prevalence (10%) and intensity (1.0). There were no significant changes in prevalence or intensity in 2006. Antibody titres and seroprevalence increased from 1.37 U μl−1 and 10% at transfer in March 2005 to reach a peak in December 2005 of 25.86 U μl−1 (SE = 6.26 U μl−1) and 66.66%. No significant changes were observed in antibody titres for the same cohort of fish during 2006. Parasitological and serological values from Cohort2005 were compared to a 2006 cohort (Cohort2006) in March 2006 and August 2006 to determine if prior infection in Cohort2005 elicited any protection against infection in 2006. Although significant differences were not observed in intensities between cohorts it was shown that Cohort2005 had significantly lower abundances and prevalences of blood fluke infection than Cohort2006. Although there was no significant difference in mean antibody titres between cohorts in March 2006, the mean antibody titre of Cohort2006 was significantly greater than that of Cohort2005 in August 2006. No significant differences were observed in seroprevalence. This is one of the few studies to demonstrate the development of acquired resistance in fish against a parasite in an aquaculture environment under natural infection conditions. 相似文献
5.
Production of clavulanic acid (CA) by Streptomyces clavuligerus in a shake-flask culture increased from 92 to 180 mg l−1 with an increased O2 transfer efficiency (0.039 → 0.058 s−1), which maintained the redox potential values above −250 mV. Compared with traditional measures, such as dissolved O2 concentration and respiratory activity, the redox potential can easily be determined and correlates closely with CA production.
It can therefore be used to monitor microbial activities during biosyntheses of secondary metabolites.
Revisions requested 5 April 2005 and 19 July 2005; Revisions received 19 July 2005 and 9 September 2005 相似文献
6.
Racemic diisophorone (500 mg) was converted by Cephalosporium aphidicola and Neurospora crassa over 10 days at 25 °C to 8β-hydroxydiisophorone in yields of 10% (52 mg) and 20% (103 mg), respectively. The structure was established by IR, specific rotation, mass spectral, 1D and 2D-NMR studies.Revisions requested 2 March 2005 and 21 April 2005; Revisions received 8 April 2005 and 10 May 2005 相似文献
7.
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9.
Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l−1 was obtained in batch culture with an initial dry cell weight of 126 mg l−1 and with aeration rate of 1.2 l air min−1 l−1 culture, nitrate at 1.5 mM and phosphate at 0.17 mM. The yield of rtPA was 56 μg g−1 dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.Revisions requested 27 January 2005 and 14 April 2005; Revisions received 6 April 2005 and 17 May 2005 相似文献
10.
The changes in the marriage structure with respect to the age at marriage, ethnicity, and spouses’ birthplaces during the
period of time corresponding to two generations have been analyzed in the rural population of Shors of Tashtagolskii raion
of Kemerovo oblast. In general, the Shor population had a high assortative marriage rate with respect to these parameters
in the period studied, although there was a temporary tendency to wards its decrease. The ages of marriage for both the male
and the female Shor populations in the years 2000–2005 were significantly older than in 1940–1945 and 1970–1975. The age-assortative
marriage rate was r = 0.60 in 1940-1945, r = 0.73 in 1970–1975, and r = 0.66 in 2000–2005. The birthplace-assortative marriage rate decreased from 79.63% in 1970–1975 to 70.64% in 2000–2005.
The ethnic assortative marriage rate of Shors steadily decreased during the time interval studied; it was 96.92, 89.95, and
80.98% in 1940–1945, 1970–1975, and 2000–2005, respectively, for the total rural population of Tashtagolskii raion. 相似文献
11.
Perveen Z Ando H Ueno A Ito Y Yamamoto Y Yamada Y Takagi T Kaneko T Kogame K Okuyama H 《Biotechnology letters》2006,28(3):197-202
A thraustochytrid-like microorganism (strain 12B) was isolated from the mangrove area of Okinawa, Japan. On the basis of its
ectoplasmic net structure and biflagellate zoospores we determined strain 12B to be a novel member of the phylum Labyrinthulomycota
in the kingdom Protoctista. When grown on glucose/seawater at 28 °C, it had a lipid content of 58% with docosahexaenoic acid
(DHA; 22:6 n−3) at 43% of the total fatty acids. It had a growth rate of 0.38 h−1. The DHA production rate of 2.8 ± 0.7 g l−1 day−1 is the highest value reported for any microorganism.
Received 7 October 2005; Revisions requested 7 October 2005; Revisions received 15 November 2005; Accepted 15 November 2005 相似文献
12.
Khattar SK Bora RS Priyadarsiny P Gupta D Khanna A Narayanan KL Babu V Chugh A Saini KS 《Biotechnology letters》2006,28(2):121-129
cDNAs encoding for five mAChR subtypes (M1–M5) were cloned under different promoters in various eukaryotic vectors and each
subtype was expressed in different mammalian cell lines. CHO-K1 cell line was the best for generating stable cell lines expressing
muscarinic receptors. Immunofluorescence and flow cytometry revealed that expression of M1–M5 was primarily localized on the
cell membrane. Western blotting and radio-ligand binding studies revealed that expression of each receptor was stable at higher
passages.
These authors contributed equally to this work.
Received 22 September 2005; Revisions requested 5 October 2005; Revisions received 2 November 2005; Accepted 4 November 2005 相似文献
13.
β-Glucosidase is frequently used to supplement cellulase preparations for hydrolysis of cellulosic and lignocellulosic substrates
in order to accelerate the conversion of cellobiose to glucose. Typically, commercial cellulase preparations are deficient
in this enzyme and accumulation of cellobiose leads to product inhibition. This study evaluates the potential for recycling
β-glucosidase by immobilization on a methacrylamide polymer carrier, Eupergit C. The immobilized β-glucosidase had improved
stability at 65 °C, relative to the free enzyme, while the profile of activity versus pH was unchanged. Immobilization resulted
in an increase in the apparent Km from 1.1 to 11 mm and an increase in Vmax from 296 to 2430 μmol mg−1 min−1. The effect of immobilized β-glucosidase on the hydrolysis of cellulosic and lignocellulosic substrates was comparable to
that of the free enzyme when used at the same level of protein. Operational stability of the immobilized β-glucosidase was
demonstrated during six rounds of lignocellulose hydrolysis.
Received 22 August 2005; Revisions requested 20 September 2005; Revisions received 8 November 2005; Accepted 10 November 2005 相似文献
14.
Samuilov VD 《Biochemistry. Biokhimii?a》2005,70(2):246-250
Evolutionary aspects of bioenergetics are considered. These include the origin of the first organisms, UV-protection and the beginnings of anoxygenic photosynthesis, the electron donor problem of life and the appearance of oxygenic photosynthesis, oxygen danger and strategies of defense, and the role of oxygen in programmed cell death.Translated from Biokhimiya, Vol. 70, No. 2, 2005, pp. 302–307.Original Russian Text Copyright © 2005 by Samuilov.This revised version was published online in April 2005 with corrections to the post codes. 相似文献
15.
Eysenbach G 《PLoS biology》2006,4(5):e157
Open access (OA) to the research literature has the potential to accelerate recognition and dissemination of research findings, but its actual effects are controversial. This was a longitudinal bibliometric analysis of a cohort of OA and non-OA articles published between June 8, 2004, and December 20, 2004, in the same journal (PNAS: Proceedings of the National Academy of Sciences). Article characteristics were extracted, and citation data were compared between the two groups at three different points in time: at “quasi-baseline” (December 2004, 0–6 mo after publication), in April 2005 (4–10 mo after publication), and in October 2005 (10–16 mo after publication). Potentially confounding variables, including number of authors, authors' lifetime publication count and impact, submission track, country of corresponding author, funding organization, and discipline, were adjusted for in logistic and linear multiple regression models. A total of 1,492 original research articles were analyzed: 212 (14.2% of all articles) were OA articles paid by the author, and 1,280 (85.8%) were non-OA articles. In April 2005 (mean 206 d after publication), 627 (49.0%) of the non-OA articles versus 78 (36.8%) of the OA articles were not cited (relative risk = 1.3 [95% Confidence Interval: 1.1–1.6]; p = 0.001). 6 mo later (mean 288 d after publication), non-OA articles were still more likely to be uncited (non-OA: 172 [13.6%], OA: 11 [5.2%]; relative risk = 2.6 [1.4–4.7]; p < 0.001). The average number of citations of OA articles was higher compared to non-OA articles (April 2005: 1.5 [SD = 2.5] versus 1.2 [SD = 2.0]; Z = 3.123; p = 0.002; October 2005: 6.4 [SD = 10.4] versus 4.5 [SD = 4.9]; Z = 4.058; p < 0.001). In a logistic regression model, controlling for potential confounders, OA articles compared to non-OA articles remained twice as likely to be cited (odds ratio = 2.1 [1.5–2.9]) in the first 4–10 mo after publication (April 2005), with the odds ratio increasing to 2.9 (1.5–5.5) 10–16 mo after publication (October 2005). Articles published as an immediate OA article on the journal site have higher impact than self-archived or otherwise openly accessible OA articles. We found strong evidence that, even in a journal that is widely available in research libraries, OA articles are more immediately recognized and cited by peers than non-OA articles published in the same journal. OA is likely to benefit science by accelerating dissemination and uptake of research findings. 相似文献
16.
E. V. Balushkina 《Inland Water Biology》2009,2(4):355-363
In the years 1994–2005, the values of the integrated index IP’ at some stations of the Neva Bay changed from 38.1 to 81.9%, water quality changed from class 3 to class 5, and the states
of some areas of the ecosystem that were evaluated as “tense” went to “catastrophic.” The integrated mean assessment of water
quality according to the IP’ index over the entire Neva Bay throughout 12 years (1994–2005) remained relatively stable, waters were assessed as “polluted”
(fourth class), and the state of the ecosystem was considered “critical.” The state of the eastern part of the Gulf of Finland
in 1994–2005 was less favorable. The species diversity of zoobenthos in the resort zone of the eastern part of the Gulf of
Finland is considerably lower than in the Neva Bay. Waters of the resort zone of the eastern part of the Gulf of Finland in
1994–2005 were assessed as one class lower than in the Neva Bay, i.e., as “polluted-dirty” (fourth-fifth class), and the state
of the ecosystem was assessed as being in a “crisis.” In the resort zone, there was a decline in species diversity and abundance
and biomass of benthic animals; i.e., all characteristics of the degradation of benthic animal communities were observed. 相似文献
17.
Characteristics and probable functions of morphogens of the freshwater polyp, hydra Hydra sp., are considered in the review.Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 1, 2005, pp. 3–11.Original Russian Text Copyright © 2005 by Kukalev. 相似文献
18.
Tao Bian Hongxia Yan Liping Shen Feng Wang Shuang Zhang Yanqiang Cao Shuo Zhang Yong Zhang Shengli Bi 《Journal of virology》2013,87(22):12196-12206
A nationwide hepatitis B virus (HBV) vaccination program was implemented in China starting in 1992. To study the change in HBV variant prevalence with massive immunization, large HBV surface protein (LHBs) genes from HBV surface antigen (HBsAg)-positive sera were amplified and sequenced. The prevalences of LHBs mutants were compared between the 1992 and 2005 surveys in child and adult groups. The prevalence of “α” determinant mutants in the children increased from 6.5% in 1992 to 14.8% in 2005, where the G145R mutant occurred most frequently. In contrast, mutation frequencies showed little difference between 1992 (9.4%) and 2005 (9.9%) in adults. Moreover, compared to the 1992 survey, the child group surface (S) protein mutation frequency specifically increased (P = 0.005) in the 2005 survey, but the pre-S region mutation frequency did not show a significant difference (P > 0.05). However, the mutation frequency in the adult group increased in both the pre-S and S regions. Furthermore, the frequencies of the disease-related pre-S2 deletion and start codon mutations were significantly higher in the adult groups than in the child groups in both the 1992 and 2005 surveys (P < 0.01). Massive immunization enhances the HBV S protein mutation; the prevalence of LHBs mutants, particularly disease-related mutants, tends to increase with patient age. 相似文献
19.
New polymethylene nucleoside analogues with a β-dioxo function in the ω-position of their hydro-carbon chain, [7-(2-oxocyclohexyl)-7-oxoheptyl]purines, were synthesized, and their physicochemical properties were studied.__________Translated from Bioorganicheskaya Khimiya, Vol. 31, No. 3, 2005, pp. 288–294.Original Russian Text Copyright © 2005 by Kritzyn, Vepsalainen, Komissarov.For communication III, see [1]. 相似文献
20.
Over-production of human soluble B lymphocyte stimulator (hsBLyS) was carried out with four different fed-batch culture strategies
using lactose as inducer, instead of IPTG, in a fed-batch culture of Escherichia coli. As lactose acted as both inducer and carbon source, the best and simplest culture strategy was direct feeding of lactose
after batch culture, thereby giving hsBLyS at 3.7 g l−1 and a productivity of 0.11 g l−1 h−1.
Revisions requested 1 September 2005 and 11 November 2005; Revisions received 7 November 2005 and 4 January 2006 相似文献