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1.
The effects of sucrose (S) and pectin (P) concentrations and the ratio between two distinct pectins (R) on the rheological behavior of diluted pectin systems were evaluated simultaneously using the surface response methodology.
The systems were composed of a mixture of two high methoxy pectins with different degree of methyl esterification values (HM1/HM2)
and of a mixture of a high-methoxy with an amidated low-methoxy pectin (HM1/LMA). For the HM1/HM2 systems, the multivariate
analysis showed that the sucrose and pectin concentrations exerted statistically significant (p < 0.05) linear effects on the consistency index k and viscosity, the influence of pectin being about five times higher than that of sucrose. The pectin concentration and the
ratio between the different pectins were shown to be significant with respect to the rheological parameters of the HM1/LMA
systems. Evaluating the influence of the ratio between the different pectins, a synergistic effect on the structure reinforcement
was observed when mixing HM1 and LMA in similar proportions, indicating the importance of the presence of hydrophobic interactions
between methyl ester groups in addition to the stronger hydrogen bonding in junction zone stabilization. In general, the conditions
in which hydrogen bonds were favored in relation to hydrophobic interactions led to systems with higher pseudoplasticity. 相似文献
2.
A. B. Shrivatav K. P. Singh S. K. Mittal P. K. Malik 《European Journal of Wildlife Research》2012,58(1):365-367
Haematological and biochemical studies were conducted on 12 clinically healthy tigers of Central India. The range and mean
(with one standard deviation), respectively for the parameters examined were: red blood cells, 4.66 to 9.15, 7.9 ± 1.42, 106/μl; haemoglobin, 7.8 to 13.8, 12.8 ± 1.65 g/dl; packed cell volume, 36 to 45, 38 ± 2.54; icterus index, 2 to 5, 2 ± 1.51 U;
erythrocyte sedimentation rate, 14 to 26, 21 ± 4.21 mm at 1 h; white blood cells, 6.2 to 11.05, 8.5 ± 1.49, 103/μl; neutrophils, 57 to 75, 60 ± 5.08%; lymphocytes, 18 to 35, 30 ± 4.56%; monocytes, 2 to 6, 5 ± 1.21%; eosinophils, 2 to
6, 4 ± 1.3; basophils, 0 to 4, 1 ± 1.21; plasma albumin, 2.1 to 4.6, 3.5 ± 0.99 mg/dl; total protein, 3.7 to 8.7, 6.4 ± 1.88 mg/dl;
total bilirubin, 0.4 to 3.2, 1.9 ± 1.21 mg/dl; creatinine, 1.6 to 4.6, 2.90 ± 1.03 mg/dl; blood urea nitrogen, 6.5 to 48.2,
27.90 ± 13.77 mg/dl; glutamic pyruvic transaminase, 21.2 to 109.0, 67.88 ± 27.84 IU/L and glutamic oxaloacetate transaminase,
14.4 to 84, 57.96 ± 17.27 IU/L; index conspicuous erythrocyte sedimentation rate; absence of reticulocytes and predominance
of neutrophils. 相似文献
3.
Selenium concentrations in the blood of 112 (56 females and 56 males) normal subjects, from different regions of the Punjab
(Pakistan), have been determined using the technique of inductively coupled plasma-mass spectrometry. The whole blood selenium
concentrations were found to be 452 ± 12 ppb (parts per billion or nano-gram of Se per gram freeze-dried blood or 96 ± 3 μg/L
), with 470 ± 16 ppb (or 100 ± 4 μg/L) in female and 435 ± 16 ppb (or 92 ± 4 μg/L) in male population. Compared with other
populations of the world, these levels are amongst the lowest. 相似文献
4.
Kumar A Singhal NK Ramanujam B Mitra A Rameshwaram NR Nadimpalli SK Rao CP 《Glycoconjugate journal》2009,26(4):495-510
Several C1-imino conjugates of d-galactose, d-lactose and d-ribose, where the nitrogen center was substituted by the salicylidene or naphthylidene, were synthesized and characterized.
Similar C2-imino conjugates of d-glucose have also been synthesized. All the glyco-imino-conjugates, which are transition state analogues, exhibited 100%
inhibition of the activity towards glycosidases extracted from soybean and jack bean meal. Among these, a galactosyl-napthyl-imine-conjugate
(1c) showed 50% inhibition of the activity of pure α-mannosidase from jack bean at 22 ± 2.5 μM, and a ribosyl-naphthyl-imine-conjugate
(3c) showed at 31 ± 5.5 μM and hence these conjugates are potent inhibitors of glycosidases. The kinetic studies suggested non-competitive
inhibition by these conjugates. The studies are also suggestive of the involvement of aromatic, imine and carbohydrate moieties
of the glyco-imino-conjugates in the effective inhibition. The binding of glyco-imino-conjugate has been established by extensive
studies carried out using fluorescence emission and isothermal titration calorimetry. The conformational changes resulted
in the enzyme upon interaction of these derivatives has been established by studying the fluorescence quench of the enzyme
by KI as well as from the secondary structural changes noticed in CD spectra. All these studies revealed the difference in
the binding strengths of the naphthylidene vs. salicylidene as well as galactosyl vs. lactosyl moieties present in these conjugates. The differential inhibition of these glyco-conjugates has been addressed by
quantifying the specific interactions present between the glyco-conjugates and the enzyme by using rigid docking studies.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
5.
The main objective of this investigation was to examine physiological changes occurring within the olive flounder, Paralichthys olivaceus, in the presence of continuous stress factors. The experiment gave continuous stress, and each stress step is as follows:
water level reduction (S1), selection process (S2), confinement (S3), air exposure (S4), transportation (S5), and storing
in volume 0.5-t (S6) and 50.0-t (S7) rearing tanks for 24 h after transportation. The cortisol concentration showed a trend
to continuously rise in response to consecutive stress from transportation from 7.4 ± 0.6 ng/ml in an experimental opening.
The concentration showed the highest level, 25.3 ± 4.4 ng/ml, after confinement stress. Glucose concentration in S3 and S4
were increased significantly to 71.0 ± 13.0 and 78.7 ± 7.0 mg/dl, respectively (P < 0.05). Lactic acid was increased significantly from 0.5 ± 0.1 mM (S0) to 9.0 ± 1.2 mM (S4), but it did not recover until
24 h (7.0 ± 1.0 mM) after transportation (S6 and S7) (P < 0.05). Some contents displayed recovery within 24 h, but a longer time would be required for normal physical metabolism
to resume after fish experienced stress. Therefore, it would be endangering the survival of this species if the transport
were repeated within a 24-h period. 相似文献
6.
Rajamani Karthikeyan T. Manivasagam P. Anantharaman T. Balasubramanian S. T. Somasundaram 《Journal of applied phycology》2011,23(2):257-263
The present study was designed to investigate the prophylactic effect of extracts of the brown alga Padina boergesenii against potent nephrotoxic agent ferric nitrilotriacetate (Fe-NTA), in blood circulation of rats. Administration of Fe-NTA
for seven consecutive days significantly enhanced lipid peroxidation accompanied with reduction in glutathione content. Together
with this, the level of antioxidant enzymes, glutathione peroxidase, superoxide dismutase, and catalase was significantly
(P < 0.05) diminished. Pretreatment of rats with P. boergesenii (150 mg kg−1 body weight) reversed Fe-NTA-induced oxidative damage in lipid peroxidation and glutathione content significantly (P < 0.05). Further, the activity of antioxidant enzymes was also restored significantly. In order to assess the role of polyphenolic
components in the relevant activity, phenolic contents of the extract was found to be 1.78 ± 0.02% in the methanol extract
and 1.30 ± 0.30% in the diethyl ether extract. Hence, the present results confirm that the brown alga P. boergesenii preclude its role in Fe-NTA-induced oxidative damage and hyperproliferative response in circulation. 相似文献
7.
María Isabel Burgos Alves Francisco Avilés Plaza Rebeca Martínez-Tomás María Sánchez-Campillo Elvira Larqué Francisca Pérez-Llamas Pedro Martínez Hernández Soledad Parra Pallarés 《Journal of physiology and biochemistry》2010,66(3):221-227
The biological effects of oxidized LDL (oxLDL) may contribute to initiation and progression of the atherosclerotic process,
and the association between cardiovascular disease and oxidation of LDL has been largely demonstrated. The objectives of this
study were to establish the reference values of oxidative stress biomarkers in a young healthy Spanish population to determine
the concentration of oxLDL and its relationship with lipid profile and with these biomarkers. oxLDL, F2-isoprostanes, protein carbonyls (PC), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) were determinate by ELISA in 72 healthy subjects.
Antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GR) were carried
out on a Hitachi 912 analyzer; lipid profile were assayed using automated systems (Cobas 711, Roche Diagnostics). All statistics
were analyzed by using SPSS for Windows 15.0. SPSS Inc, Chicago, IL, USA. (Normal mean reference values): oxLDL (63.23 ± 16.23
U/L), (Male/Female 68.06 ± 17.69/58.39 ± 13.6 U/L), F2-isoprostanes (2.26 ± 0.9 μg/g creatinine), PC (0.34 ± 0.15 nmol/mg), 8-OHdG (23.27 ± 10.58 ng/ml), SOD (931.97 ± 271.09 U/g
Hb), GR (46.56 ± 11.68 U/L), GPx (27.58 ± 6.89 U/gHb (Male/Female 25.91 ± 5.03/29.2 ± 8.07 U/L)). OxLDL (63.23 U/L) was significantly
(p < 0.05) positively correlated with BMI (22.53 Kg/m2), total cholesterol (175.79 mg/dl), triglycerides (87.58 mg/dl), LDL cholesterol (96.25 mg/dl), and uric acid (4.78 mg/dl),
while negatively correlated with HDL-cholesterol (62.25 mg/dl). We have found different correlation between oxLDL and isoprostanes
by gender with the rest of parameters. Normal reference values have been found significantly different for oxLDL and GPx by
gender. Oxidized LDL is correlated with lipid profile but not with the oxidative stress biomarkers. Urinary isoprostanes are
positively correlated with triglycerides and negatively with GR and GPx. 相似文献
8.
Jagmohan S. Negi Pramod Singh Geeta J. nee Pant Mohan S. Maniyari Rawat 《Biological trace element research》2010,136(3):364-371
Concentration level of nine elements viz. Zn, Cu, Mn, Fe, Co, Na, K, Ca, and Li were determined in leaves and roots of Asparagus curillus (Buch.-Ham.) ex Roxb. collected from four different altitudes in three seasons by atomic absorption spectroscopy. The overall
concentration of K was found to be highest, whereas the level of Cu was lowest. The maximum concentrations of Zn, Cu, Mn,
Fe, Co, Na, K, Ca, and Li were found to be 97.0 ± 1.5, 28.0 ± 7.0, 44.0 ± 7.3, 1138.0 ± 18.5, 91.0 ± 6.2, 381.0 ± 7.8, 9508.0 ± 7.8,
3076.0 ± 6.4, and 78.0 ± 4.6 mg/kg, respectively. 相似文献
9.
E. é. Kolesnikova V. I. Nosar’ I. N. Man’kovskaya T. V. Serebrovskaya 《Neurophysiology》2012,44(1):14-19
Using a polarographic technique, we studied the peculiarities of energy metabolism in neurons of the rat brainstem structures
related to normal physiological aging. Experiments were carried out under in vitro conditions on mitochondrial (MCh) suspensions prepared from the brainstem cells of young and old rats. In addition, we examined,
using the same technique, the parameters of oxidative phosphorylation in analogous MCh suspension under conditions of experimental
MCh dysfunction induced by single systemic injection of rotenone into young animals. In the case where we used a succinate + rotenone
mixture as the substrate for oxidation, the intensity of ADP-stimulated respiration (V3) in preparations from brainstem neurons
of old animals was significantly smaller (against the background of a decrease in the efficacy of respiration control, V3/V4).
If a mixture glutamate + malate was used as the substrate for oxidation, the V3 and the efficacy of phosphorylation (ADP/O)
decreased significantly. The experimental MCh dysfunction resulted in the lowering of practically all parameters of oxidation
and phosphorylation under conditions of oxidation of glutamate + malate, as well as V3, V3/V4, and ADP/O, in the case where
we used succinate + rotenone as the substrate for oxidation. Less expressed changes in the recorded indices upon oxidation
of succinate + rotenone were indicative of activation of the succinate oxidase pathway; this preserved the electrotransport
function of the respiratory chain in the MCh on a certain level and the ability of the latter to provide oxidative phosphorylation. 相似文献
10.
Daisuke Sugimori 《Applied microbiology and biotechnology》2009,82(2):351-357
To develop a microbial treatment of edible oil-contaminated wastewater, microorganisms capable of rapidly degrading edible
oil were screened. The screening study yielded a yeast coculture comprising Rhodotorula pacifica strain ST3411 and Cryptococcus laurentii strain ST3412. The coculture was able to degrade efficiently even at low contents of nitrogen ([NH4–N] = 240 mg/L) and phosphorus sources ([PO4–P] = 90 mg/L). The 24-h degradation rate of 3,000 ppm mixed oils (salad oil/lard/beef tallow, 1:1 w/w) at 20°C was 39.8% ± 9.9% (means ± standard deviations of eight replicates). The highest degradation rate was observed at
20°C and pH 8. In a scaled-up experiment, the salad oil was rapidly degraded by the coculture from 671 ± 52.0 to 143 ± 96.7 ppm
in 24 h, and the degradation rate was 79.4% ± 13.8% (means ± standard deviations of three replicates). In addition, a repetitive
degradation was observed with the cell growth by only pH adjustment without addition of the cells. 相似文献
11.
Young-Jae Kim Oyunbileg Galindev Jun Han Sei Su-Mi Bae Hosub Im Lanying Wen Young Rok Seo Woong Shick Ahn 《Biological trace element research》2009,131(2):103-109
Of trace elements in the serum of living organisms, selenium (Se) is an essential mineral and plays the role of an antioxidant
as selenoproteins protecting the organism against oxidative damage induced by hydrogen peroxide, other lipid hydroperoxides,
and their derivatives. The aim of this study was to determine the mean serum Se levels in healthy Korean volunteers (50 males
and 50 females) by using an inductively coupled plasma-mass spectrometry method. The samples were collected at the Health
Promotion Centre of Kangnam St. Mary’s Hospital, College of Medicine, the Catholic University of Korea, Kangnam District,
Seoul in accordance with procedures approved by the Institutional Review Board of the Catholic University of Korea. The mean
serum Se level in healthy subjects was 112.05 ± 30.42 μg/l. For gender, it was 120.81 ± 27.37 μg/l for females and 103.29 ± 31.05 μg/l
for males. From the study result, there was a significant difference between the mean Se concentrations of gender groups (p = 0.0035). Also, the study indicated no effect of age on Se levels (p > 0.05) in the healthy individuals. 相似文献
12.
Adewale Adewuyi Rotimi A Oderinde B. V. S. K. Rao R. B. N. Prasad B. Anjaneyulu 《Bioenergy Research》2012,5(3):713-718
Self-sufficiency in energy requirement is critical to the success of any developing economy. Apart from the search for alternatives, there is a need to achieve energy independence, directing much focus on biofuels. Biodiesel is simple to use, biodegradable, nontoxic, and essentially free of sulfur and aromatics. Oil was extracted from the seeds of Blighia unijugata and Luffa cylindrica, subjected to chemical characterization and biodiesel production. The oil yield from the seed of B. unijugata was 50.82 ± 1.20% while that of L. cylindrica was 39.10 ± 0.20%. The biodiesel produced had ester content above 98%. The flash point of the biodiesel from B. unijugata and L. cylindrica was above 120°C while the phosphorus content was also below 1 ppm in both cases. The oxidative stability of B. unijugata was 44.30 ± 0.30 h, while that of L. cylindrica was lower than this value due to its high unsaturation. The copper strip corrosion value of the biodiesel was also found to be 1A. This study showed that the high free fatty acid content of B. unijugata and L. cylindrica seed oil can be reduced in a one-step pretreatment of esterification reaction using H2SO4 as catalyst thus reducing the problem of soap formation encountered when using oil with high free fatty acid for the production of biodiesel. 相似文献
13.
Ines Fürtbauer Oliver Schülke Michael Heistermann Julia Ostner 《International journal of primatology》2010,31(4):501-517
Information on basic reproductive parameters and life-history traits is crucial for the understanding of primate evolution,
ecology, social behavior, and reproductive strategies. Here, we report 4 yr of data on reproductive and life-history traits
for wild female Assamese macaques (Macaca assamensis) at Phu Khieo Wildlife Sanctuary, northeastern Thailand. During 2 consecutive reproductive seasons, we investigated reproductive
behavior and sexual swelling size in 16 females and collected 1832 fecal samples. Using enzyme immunoassays, we measured fecal
estrogen and progesterone metabolites to assess ovarian activity and timing of ovulation and to ascertain conceptions and
pregnancies. Timing of reproduction was strictly seasonal (births: April–July, 86% in April–June, 4 yr, n = 29; conceptions: October–February, 65% in December–January, 2 yr, n = 17). Females showed no cyclic ovarian activity outside the mating season and conceived in their first or second cycle (mean:
1.2 cycles to conception, n = 13). Gestation length was on average 164.2 d (range: 158–170, n = 10), and females had their first infant at an age of 5 yr (n = 4). Interbirth intervals were bimodally distributed, with females giving birth on average every 13.9 or 23.2 mo. Shorter
interbirth intervals were linked to early parturition within the birth season. Most females displayed subcaudal sexual swellings
which, however, did not reliably indicate female reproductive status or fertility. Overall, our results fall within the range
of findings reported for other macaque species. These results thus add to the growing body of information available for wild
macaques, facilitating comparative studies for a better understanding of interspecific differences in social and reproductive
patterns. 相似文献
14.
Miriam Altstein Tal Gabay Orna Ben-Aziz Shai Daniel Irina Zeltser Chaim Gilon 《Invertebrate neuroscience : IN》1999,4(1):0033-0040
The binding of [3H]tyrosyl-PBAN28-33NH2 to pheromone gland membranes of the moth Heliothis peltigera was investigated. The study describes the development of a pheromone biosynthesis-activating neuropeptide (PBAN) radioreceptor
assay and demonstrates the presence of a putative PBAN binding site on the pheromone gland. It also describes synthesis of
a radioligand and optimization of binding conditions with respect to membrane preparation, number of gland equivalents, kinetics
of ligand binding and composition of the binding solution. Binding was found to be optimal when membranes were freshly prepared
from frozen glands, incubated at a concentration of one gland equivalent per reaction tube in the presence of 10 mM HCO3
− ions. Equilibrium of ligand binding was obtained after 20 min. Presence of other components such as NaCl, KCl or SH reagents
did not have any effect on binding. Binding was found to be saturable, with a Kd of 5.73 ± 1.05 × 10−6 M and a Bmax of 1.85 ± 0.22 nmol/mg protein. Binding was effectively displaced by unlabeled PBAN1-33NH2 and PBAN28-33ΝΗ2 with a Ki of 4.3 ± 1.1 × 10−6 M and 4.9 ± 2.6 × 10−6 M, respectively.
Accepted: 4 February 1999 相似文献
15.
16.
Quantitative analysis of follicular fluid and granulosa cells from small, medium and large antral atretic follicles of goat
(Capra hircus) ovaries was conducted to study the alterations in trace elements viz zinc (Zn), copper (Cu), manganese (Mn), and iron (Fe).
The zinc content was lower in the follicular fluid (0.993 ± 0.001, 0.935 ± 0.002, 1.321 ± 0.001 μg/ml) and granulosa cells
(0.867 ± 0.002, 0.801 ± 0.001, 1.073 ± 0.002 μg/mg) of small, medium, and large antral atretic follicles respectively than
their respective controls. Copper quantity was higher in the follicular fluid (0.113 ± 0.001, 0.163±0.001 0.{163}\pm 0.00{1} , 0.224 ± 0.001 μg/ml) and granulosa cells (0.094 ± 0.001, 0.114 ± 0.001, 0.182 ± 0.001 μg/mg) from small, medium, and large
antral atretic follicles respectively than their respective controls. Similarly, iron and manganese was also found higher
in the follicular fluid and granulosa cells of small, medium, and large antral atretic follicles than their respective controls.
The present study provides the basic data on trace elements that can be safely used as atretic marker and will find use in
in vitro studies for fertility improvement plan. Thus, help in elevating the number of ovulations and screening of follicles
to enhance the success rate in vivo and in vitro fertilization and embryo transfer technology. 相似文献
17.
To screen stimulators from Chinese medicinal insects for mycelial growth and polysaccharides production of Ganoderma lucidum, G. lucidum was inoculated into the media with and without supplementation of medicinal insect extracts. The ethyl acetate extract of
Eupolyphaga sinensis at 55 mg l−1 lead to significant increase in both biomass and intracellular polysaccharides (IPS) concentration from 8.53 ± 0.41 to 14.16 ± 0.43
and 1.28 ± 0.09 to 2.13 ± 0.11 g l−1, respectively. In addition, the ethyl acetate extract of Catharsius molossus at 55 mg l−1 significantly enhanced extracellular polysaccharides (EPS) production; the EPS yield increased from 350.9 ± 14.1 to 475.1 ± 15.3 mg
l−1. There were no new components in the two types of polysaccharides obtained by the addition of the insect extracts. 相似文献
18.
The contribution of ammonia-oxidizing archaea (AOA) to nitrogen removal in wastewater treatment plants (WWTPs) remains unknown.
This study investigated the abundance of archaeal (AOA) and bacterial (ammonia-oxidizing bacteria (AOB)) amoA genes in eight of Bangkok’s municipal WWTPs. AOA amoA genes (3.28 × 107 ± 1.74 × 107–2.23 × 1011 ± 1.92 × 1011 copies l−1 sludge) outnumbered AOB amoA genes in most of the WWTPs even though the plants’ treatment processes, influent and effluent characteristics, removal efficiencies,
and operation varied. An estimation of the ammonia-oxidizing activity of AOA and AOB suggests that AOA involved in autotrophic
ammonia oxidation in the WWTPs. Statistical analysis shows that the numbers of AOA amoA genes correlated negatively to the ammonium levels in effluent wastewater, while no correlation was found between the AOA
amoA gene numbers and the oxygen concentrations in aeration tanks. An analysis of the AOB sequences shows that AOB found in the
WWTPs limited to only two AOB clusters which exhibit high or moderate affinity to ammonia. In contrast to AOB, AOA sequences
of various clusters were retrieved, and they were previously recovered from a variety of environments, such as thermal and
marine environments. 相似文献
19.
Emanuelli M Cecati M Sartini D Stortoni P Corradetti A Giannubilo SR Turi A Tranquilli AL 《Cell stress & chaperones》2009,14(2):193-197
AHSP inhibits cellular production of the reactive oxygen species. Reduced AHSP indicates reduced protection against oxidative
stressors. Our objective was to investigate AHSP levels in recurrent miscarriage (RM). Trophoblast was collected from women
of 10 weeks gestation: voluntary abortion controls (VA, n = 10); spontaneous first miscarriage with subsequent normal pregnancy (SMSN, n = 15) or with subsequent miscarriage (SMSM, n = 5); RM previously investigated (RMPS, n = 5) or not previously investigated (RM, n = 5). AHSP mRNA and protein were determined using real-time quantitative polymerase chain reaction (PCR) and Western blot,
respectively. One-way ANOVA was performed to assess statistical significance (p < 0.05). ahsp mRNA levels were maximally reduced in RM and RMPS (8.0 × 10−6 ± 1.3 and 8.1 × 10−6 ± 0.7, respectively) compared with SMSN and VA (16.1 × 10−6 ± 2.3 and 26.1 × 10−6 ± 2.7, respectively). SMSM showed levels significantly reduced as well (9.0 × 10−6 ± 2.3). In RM, a reduced defense from oxidative stressors is evident at first miscarriage, identifying women at high risk
for subsequent eventful pregnancy. Reduced AHSP may identify women at risk of experiencing further miscarriages.
Monica Emanuelli and Monia Cecati contributed equally to this paper. 相似文献
20.
Morphological and Genetic Characterization of <Emphasis Type="Italic">Saimiri boliviensis</Emphasis>
Eliana Ruth Steinberg Mariela Nieves Marina Sofía Ascunce Ana María Palermo Marta Dolores Mudry 《International journal of primatology》2009,30(1):29-41
The taxonomy of Saimiri is controversial because morphological characteristics, traditionally used for identification, are insufficient to distinguish
species and subspecies. Genetic studies of specimens become relevant for captive management, especially considering their
frequently unknown geographical origin. We analyzed phenotypic and genetic parameters in Saimiri spp. in Argentinean zoological gardens and biological stations to provide a more accurate taxonomic identification. We studied
27 males and 19 females of Saimiri spp. The cytogenetic analysis in mitotic metaphases corroborated a modal number of 2N = 44, XX/XY, and FN = 75 for males
and FN = 76 for females. G- and C-bands, fluorescence in situ hybridization (FISH) and the pelage coloration pattern of all the specimens corresponded to Saimiri boliviensis boliviensis. We characterized for the first time the sperm cell morphology and morphometry (mean ± SE): total length: 71.39 ± 5.40 μm;
head length: 5.71 ± 0.81 μm; head width: 3.76 ± 0.70 μm; acrosome length: 3.70 ± 0.82 μm; midpiece length: 12.20 ± 2.22 μm.
Researchers can use the characterization of the sperm morphology as another parameter for taxonomic identification that, together
with cytogenetic and molecular ones, would allow a more precise identification of individual Saimiri boliviensis boliviensis. 相似文献