共查询到20条相似文献,搜索用时 250 毫秒
1.
Yuanguang Meng Zhiqiang Wu Xiaoyun Yin Yali Zhao Meixia Chen Yiling Si Jie Yang Xiaobing Fu Weidong Han 《BMC cell biology》2009,10(1):96
Background
Oncogenesis in breast cancer is often associated with excess estrogen receptor α(ERα) activation and overexpression of its coactivators. LRP16 is both an ERα target gene and an ERα coactivator, and plays a crucial role in ERα activation and proliferation of MCF-7 breast cancer cells. However, the regulation of the functional availability of this coactivator protein is not yet clear. 相似文献2.
Silvia Kocanova Mahta Mazaheri Stéphanie Caze-Subra Kerstin Bystricky 《BMC cell biology》2010,11(1):98
Background
The estrogen receptor alpha (ERα) is found predominately in the nucleus, both in hormone stimulated and untreated cells. Intracellular distribution of the ERα changes in the presence of agonists but the impact of different antiestrogens on the fate of ERα is a matter of debate. 相似文献3.
Background
Estrogen receptors (ER) have important physiological roles in both the female and male reproductive systems. Previous studies using the estrogen receptor-α knockout mouse (αERKO) or antiestrogen treatment in adult rodents have shown that ERα is essential for normal function of the male reproductive tract. In the present study, time-response effects of the antiestrogen ICI 182,780 were determined to better understand ERα function in the adult male. 相似文献4.
Irene Kalpokas Fernando Perdigón Rodolfo Rivero Marilina Talmon Isabel Sartore Carolina Viñoles 《Acta veterinaria Scandinavica》2010,52(1):66
Background
Intrauterine infusions have been widely used for the treatment of endometritis in the mare. Nevertheless, their consequences on endocrine and endometrial molecular aspects are unknown. We studied the effect of a 1% povidone-iodine solution intrauterine infusion on progesterone levels, endometrial histology and estrogen (ERα) and progesterone (PR) receptor distribution by immunohistochemistry. 相似文献5.
Background
Somatostatin receptor (SSTR) expression is positively correlated with tumor size and inversely correlated with epidermal growth factor receptor (ErbB) levels and tumor differentiation. In the present study, we compared SSTR1-5 and ErbB1-4 mRNA and protein expression in two breast cancer cell lines: MCF-7 (ER+) and MDA-MB-231 (ERα-). 相似文献6.
7.
Oreffo RO Kusec V Virdi AS Flanagan AM Grano M Zambonin-Zallone A Triffitt JT 《Histochemistry and cell biology》1999,111(2):125-133
Estrogen deficiency at the menopause is associated with an increased rate of bone loss and subsequent risk of skeletal fracture.
Whilst cells of the osteoblastic lineage are known to express estrogen receptors, the presence of estrogen receptors in osteoclasts
remains controversial. We have examined expression of the classic estrogen receptor, estrogen receptor-alpha (ERα), during
osteoclast differentiation. In situ mRNA hybridisation with a digoxygenin-labelled riboprobe to ERα mRNA, together with immunocytochemical
analysis using a human ERα-specific monoclonal antibody demonstrated similar findings and confirmed the expression of ERα
in chondroblasts and osteoblasts from human fetal bone and mineralising human bone marrow cultures. ERα expression was detected
in human bone marrow cultures treated with 1,25(OH)2D3 and macrophage colony-stimulating factor and in macrophage cultures treated with 1,25(OH)2D3. However, in an in vitro model of human osteoclast formation, no ERα expression was observed in the osteoclasts that developed.
The human preosteoclast TCG 51 cell line showed strong expression of ERα in contrast to the low levels observed in the more
mature bone resorptive TCG 23 cell line. No expression was detectable in osteoclasts cultured from giant cell tumour of bone
(GCTB) tissue or in osteoclasts in Pagetic, GCTB, or hyperparathyroid bone tissues. In conclusion, preosteoclasts express
detectable levels of ERα, but osteoclast maturation and bone resorption is associated with loss of ERα expression. This indicates
that ERα expression and regulation may play a role in osteoclast formation.
Accepted: 4 November 1998 相似文献
8.
Noel N Kim Miljan Stankovic Tulay T Cushman Irwin Goldstein Ricardo Munarriz Abdulmaged M Traish 《BMC physiology》2006,6(1):4-9
Background
Diabetes is associated with declining sexual function in women. However, the effects of diabetes on genital tissue structure, innervation and function remains poorly characterized. In control and streptozotocin-treated female rats, we investigated the effects of diabetes on vaginal blood flow, tissue morphology, and expression of arginase I, endothelial nitric oxide synthase (eNOS) and cGMP-dependent protein kinase (PKG), key enzymes that regulate smooth muscle relaxation. We further related these changes with estrogen receptor alpha (ERα) and androgen receptor (AR) expression. 相似文献9.
10.
Massimo Mariotti Sara Castiglioni Daniela Bernardini Jeanette AM Maier 《Immunity & ageing : I & A》2006,3(1):4
Background
The functional changes associated with endothelial senescence may be involved in human aging and age-related vascular disorders. Since the inflammatory cytokine interleukin (IL-)1 inhibits endothelial growth, we evaluated the expression of IL-1α, IL-1β and their antagonist, the IL-1 receptor antagonist (IL-1ra), in endothelial in vitro senescence and quiescence. We also examined the expression of IL-1α in human senescent and progeric fibroblasts. 相似文献11.
Choijookhuu N Sato Y Nishino T Endo D Hishikawa Y Koji T 《Histochemistry and cell biology》2012,137(5):575-587
Although constipation is very common during pregnancy, the exact mechanism is unknown. We hypothesized that the involvement
of estrogen receptor (ER) in the regulation of electrolyte transporter in the colon leads to constipation. In this study,
the intestines of normal female ICR mouse and pregnant mice were examined for the expression of ERα and ERβ by immunohistochemistry
and in situ hybridization. ERβ, but not ERα, was expressed in surface epithelial cells of the proximal, but not distal, colon
on pregnancy days 10, 15, and 18, but not day 5, and the number of ERβ-positive cells increased significantly during pregnancy.
Expression of NHE3, the gene that harbors estrogen response element, examined by immunohistochemistry and western blotting,
was localized in the surface epithelial cells of the proximal colon and increased in parallel with ERβ expression. In ovariectomized
mice, NHE3 expression was only marginal and was up-regulated after treatment with 17β-estradiol (E2), but not E2 + ICI 182,780 (estrogen receptor antagonist). Moreover, knock-down of ERβ expression by electroporetically transfected siRNA
resulted in a significant reduction of NHE3 expression. These results indicate that ERβ regulates the expression of NHE3 in
the proximal colon of pregnant mice through estrogen action, suggesting the involvement of increased sodium absorption by
up-regulated NHE3 in constipation during pregnancy. 相似文献
12.
13.
3D-QSAR and molecular docking analysis were performed to explore the interaction of estrogen receptors (ERα and ERβ) with
a series of 3-arylquinazolinethione derivatives. Using the conformations of these compounds revealed by molecular docking,
CoMFA analysis resulted in the first quantitative structure-activity relationship (QSAR) and first quantitative structure-selectivity
relationship (QSSR) models predicting the inhibitory activity against ERβ and the selectivity against ERá. The q2 and R2 values, along with further testing, indicate that the obtained 3D-QSAR and 3D-QSSR models will be valuable in predicting
both the inhibitory activity and selectivity of 3-arylquinazolinethione derivatives for these protein targets. A set of 3D
contour plots drawn based on the 3D-QSAR and 3D-QSSR models reveal modifications of substituents at C2 and C5 of the quinazoline
which my be useful to improve both the activity and selectivity of ERβ/ ERα. Results showed that both the steric and electrostatic
factors should appropriately be taken into account in future rational design and development of more active and more selective
ERβ inhibitors for the therapeutic treatment of osteoporosis.
Figure Structures of ERβ binding with compounds 1aar, 1ax and 1aag obtained from molecular docking 相似文献
14.
15.
Elena Karnaukhova Yakir Ophir Loc Trinh Nimish Dalal Peter J Punt Basil Golding Joseph Shiloach 《Microbial cell factories》2007,6(1):34
Background
Human α1-proteinase inhibitor (α1-PI), also known as antitrypsin, is the most abundant serine protease inhibitor (serpin) in plasma. Its deficiency is associated with development of progressive, ultimately fatal emphysema. Currently in the United States, α1-PI is available for replacement therapy as an FDA licensed plasma-derived (pd) product. However, the plasma source itself is limited; moreover, even with efficient viral inactivation steps used in manufacture of plasma products, the risk of contamination from emerging viruses may still exist. Therefore, recombinant α1-PI (r-α1-PI) could provide an attractive alternative. Although r-α1-PI has been produced in several hosts, protein stability in vitro and rapid clearance from the circulation have been major issues, primarily due to absent or altered glycosylation. 相似文献16.
17.
Jasmin Kirchner Emese Vissi Sascha Gross Balazs Szoor Andrey Rudenko Luke Alphey Helen White-Cooper 《BMC molecular biology》2008,9(1):36
Background
Protein phosphatase 1 (PP1) is involved in diverse cellular processes, and is targeted to substrates via interaction with many different protein binding partners. PP1 catalytic subunits (PP1c) fall into PP1α and PP1β subfamilies based on sequence analysis, however very few PP1c binding proteins have been demonstrated to discriminate between PP1α and PP1β. 相似文献18.
Takakazu Oka Yoko Tamagawa Sota Hayashida Yuko Kaneda Naoki Kodama Sadatoshi Tsuji 《BioPsychoSocial medicine》2007,1(1):1-6
Background
The α-EEG anomaly during sleep, originally associated with chronic pain, is noted in several psychiatric and medical conditions and is also present in some normal subjects. The exact significance of the α-EEG anomaly is uncertain, but it has been suggested to be a nonspecific response to a variety of noxious stimuli. We propose that attachment insecurity, which is often associated with a state of hypervigilance during wakefulness, may be associated with the α-EEG anomaly during sleep. 相似文献19.
The aims of the present study were to detect the ontogeny of estrogen receptor (ERα and ERβ) and androgen receptor (AR) expressions
and their co-localization with Islet-1 in the developing dorsal root ganglia (DRG) of sheep fetuses by immunohistochemistry.
From the single staining results, the ERα immunoreactivity (ERα-ir), ERβ immunoreactivity (ERβ-ir) and AR immunoreactivity
(AR-ir) was first detected at days 90, 120 and 90 of gestation, respectively. From days 90 to 120, ERα and AR were consistently
detected in the nuclei of DRG neurons and the relative percentage (approximately 60%) of ERα-ir or AR-ir cells did not change
significantly. Moreover, there was no change in ERα expression, while a dramatic loss of AR expression was observed at birth.
From day 120 of gestation to birth, very few neurons (approximately 8%) showed nuclear ERβ immunoreactivity. The dual staining
results showed that Islet-1 was co-localized with ERα, ERβ or AR in the nuclei of DRG neurons with various frequencies, and
over 70% ERα-ir, ERβ-ir or AR-ir cells contained Islet-1. These results imply that ERs, AR and Islet-1 may be important in
regulating the differentiation and functional maintenance of some phenotypes of DRG neurons after mid-gestation in the sheep
fetus. 相似文献
20.
Verena Kriechbaumer Linda Weigang Andreas Fießelmann Thomas Letzel Monika Frey Alfons Gierl Erich Glawischnig 《BMC plant biology》2008,8(1):44