The high-energy state of the thylakoid system as indicated by chlorophyll fluorescence and chloroplast shrinkage

Certain long-term fluorescence phenomena observed in intact leaves of higher plants and in isolated chloroplasts show a reverse relationship to light-induced absorbance changes at 535 nm (“chloroplast shrinkage”).

1. 1. In isolated chloroplasts with intact envelopes strong fluorescence quenching upon prolonged illumination with red light is accompanied by an absorbance increase. Both effects are reversed by uncoupling with cyclohexylammonium chloride.

2. 2. The fluorescence quenching is reversed in the dark with kinetics very similar to those of the dark decay of chloroplast shrinkage.

3. 3. In intact leaves under strong illumination with red light in CO₂-free air a low level of variable fluorescence and a strong shrinkage response are observed. Carbon dioxide was found to increase fluorescence and to inhibit shrinkage.

4. 4. Under nitrogen, CO₂ caused fluorescence quenching and shrinkage increase at low concentrations. At higher CO₂ levels fluorescence was increased and shrinkage decreased.

5. 5. In the presence of CO₂, the steady-state yield of fluorescence was lower under nitrogen than under air, whereas chloroplast shrinkage was stimulated in nitrogen and suppressed in air.

6. 6. These results demonstrate that the fluorescence yield does not only depend on the redox state of the quencher Q, but to a large degree also on the high-energy state of the thylakoid system associated with photophosphorylation.

Relationship between energy conservation in buildings and indoor air quality

1. 1. Problems with regard to the relationship between energy conservation and indoor air quality in buildings are discussed with a brief history of legislation and practices in Japan since the oil crisis in 1973.

2. 2. In spite of energy conservation as widely advocated the sick building syndrome has hardly been manifested in most of the office spaces in Japan owing to the Law on the Assurance of Healthy Conditions in Buildings which enforced CO₂ concentration to be kept lower than 1000 ppm.

Criteria for a comfortable indoor environment in buildings

1. 1. The major purpose of buildings is to provide a healthy and comfortable environment for occupants.

2. 2. The indoor environment is a complex system including factors like thermal, visual and acoustic conditions, indoor air quality, electromagnetic fields, static electricity and vibration.

3. 3. To obtain an indoor environment that is acceptable in terms of health as well as comfort, criteria for these factors need to be established.

4. 4. The present paper gives an overview of the criteria recommended in current existing standards and guidelines.

5. 5. As most studies to date have focussed on thermal conditions and indoor air quality, these two factors are described in more detail.

Thermal responses of the fresh water turtle Mauremys caspica to step-function changes in the ambient temperature

1. 1.|The turtle Mauremys caspica cools significantly faster than it heats in air. The heating/cooling ratio is 0.49.

2. 2.|The variation of body temperature in relation to time-course in response to a step-function change of environmental temperature, fitted to a second-order system improves that of a first-order system.

3. 3.|The gradient between ambient temperature (T_a) and equilibrium body temperature (T_b) increases significantly and progressively when ambient temperature rises over 25°C.

4. 4.|At 40°C thermoregulatory hyperventilation was detected, implying an increase in air convection requirement (ventilation relative to O₂ consumption, ).

The effects of carbon dioxide concentration on oxygen evolution and fluorescence transients in synchronous cultures of Chlorella pyrenoidosa

1. 1. The steady-state fluorescence yield of Chlorella pyrenoidosa is strongly affected by CO₂ concentration: the yield is approximately 2-fold higher in the presence than in the absence of CO₂. During induction, in the presence of saturating CO₂, accelerating oxygen evolution is paralleled by rising fluorescence (M₂-P₃ transient); in the absence of CO₂, fluorescence yield remains at the low M₂ level.

2. 2. Both illumination and CO₂ content are important in determining the steady-state fluorescence yield: at lower illuminations, lower concentrations of CO₂ are required to obtain a maximum fluorescence yield.

3. 3. The slow fluorescence transients are not affected directly by pH but only indirectly through the CO₂ concentration.

4. 4. The CO₂-dependent fluorescence rise (M₂-P₃ transient) is most readily observed in cells harvested early in the light period of a synchronous culture, but it can also be elicited in cells harvested during the dark period.

5. 5. Addition of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea (DCMU) to CO₂-deprived cells raises the fluorescence yield approximately 4-fold, that is to the same high level as cells supplied with CO₂ and DCMU.

6. 6. The effects of CO₂ provide a new example of a marked parallelism between photosynthetic electron transport and fluorescence. To explain such parallelism, it seems necessary to postulate large changes in the de-excitation processes within Photosystem II units or in the distribution of excitation between Photosystems I and II.

Research on office environment evaluation and worker fatigue

1. 1. The purpose of this research was to study the relationship between indoor environment and fatigue by analyzing office workers' fatigue and evaluation of indoor environment according to occupation.

2. 2. This study concerned office workers' fatigue (before and after work), questionnaires on indoor environment, and measurement of the physical environment. The subject (562 people) were businessmen, interior designers, and salesmen.

3. 3. The result of this study showed that there was a high correlation between the office workers' feeling of fatigue and the office environment.

4. 4. This research demonstrated that the workers of each type of occupation responde differently to similar office environments.

The actual conditions of practising traditional methods of environmental control and utilization of air conditioners by the residents of detached houses in kyoto during summer

1. 1. Reducing the carbon dioxide generated by human activity is necessary for the protection of the global environment.

2. 2. Traditional methods of environmental control, e.g. watering, called uchimizu in Japan, is interesting from this point of view. Traditional methods, other than watering, still continue to be practised in Japan.

3. 3. More than 300 residents of detached houses in Kyoto city were investigated for the methods of environmental control used in summer. Investigated areas were Kitashirakawa, Nishijin and Rakusai N.T.

4. 4. Residents' who conduct traditional methods of environmental control use air conditioner (ACs) less than those who don't. Residents' attitude toward ACs have a greater effect on total time of use rather than on frequency.

5. 5. In the centre of the city, there is a tendency for residents to use ACs against their will or only as a necessity, which leads to anxiety regarding their health.

6. 6. The use of traditional methods of protection from heat have been decreasing with social change.

Relationship between cerebrovascular disease and indoor thermal environment in two selected towns in Miyagi Prefecture, Japan

1. 1. This paper describes the results of an investigation of the relationship between mortality from cerebrovascular disease (hereafter called stroke) and the indoor thermal environment in two selected towns with different socioeconomic compositions: Shiwahime, an agricultura town with relatively high stroke mortality, and Karakuwa, a fishing town with relatively low stroke mortality, using a case-control research design.

2. 2. The measurement of the indoor thermal environments were conducted and the surveyed with a questionnaire during the winter of 1986.

3. 3. The findings are summarized as detailed below.

4. 4. Room temperatures in the control households were generally higher than those in the case households by up to 1.3°C.

5. 5. The thermal conditions of the housing in the case households were a little inferior to those in the control households.

6. 6. Despite much lower room temperatures than the so-called comfort temperature, members of both the case and the control households did not express feelings of being cold in the rooms.

7. 7. It is concluded, including the survey results from other literature, that improvements in the indoor thermal environment should receive more attention with respect of the reduction in stroke mortality, particularly in areas of cold climates.

Skin temperatures, thermosensory and pleasantness estimates in case of heterogeneity in the thermal environment

1. 1. Seven thermal conditions were imposed on male sitting subjects (slightly clothed: 0.6 clo).

2. 2. A thermal mannikin was also used to determine the exact operative temperature, T₀.

3. 3. Conditions were: uniform (UN: all parameters at 24.5°C, air velocity at 0.15 ms⁻¹), heated ceiling (HC at 45°C), heated floor (HF at 34°C), cold floor (CF at 14°C), two conditions of one cold wall at 6°C (CW1 and CW2 respectively with and without air temperature compensation) and increased air velocity (AV at 0.4 ms⁻¹).

4. 4. Local skin temperatures and answers to questionnaires were obtained.

5. 5. Skin temperature variations were affected by conditions and slight T₀ changes.

6. 6. Comfort judgments were fairly well related to T₀, especially when expressed as differences between actual non-uniform environment and the uniform one.

7. 7. It is concluded that, in case of non-uniform environments close to thermoneutral zone, thermal comfort or discomfort reflects the climate alterations better than the thermal sensation does.

Activation of CO2 fixation in isolated spinach chloroplasts

1. 1. The effect of the Mg²⁺ concentration on the CO₂ fixation activity in situ in isolated and intact spinach chloroplasts upon suspension in hypotonic medium was examined. CO₂ fixation in the dark was activated 25–100 fold by 20 mM Mg²⁺ in the presence of added ATP plus either ribulose 5-phosphate or ribose 5-phosphate. 20 mM Mg²⁺-stimulated fixation only 2–3 fold in the presence of the substrate of fixation, ribulose 1,5-diphosphate. The highest Mg²⁺-stimulated rate of fixation in the dark observed with chloroplasts was 480 μmoles CO₂ fixed per mg chlorophyll per h.

2. 2. The concentration of bicarbonate at half of the maximal velocity (apparent K_m) during the Mg²⁺-stimulated fixation of CO₂ was 0.4 mM in the presence of ATP plus ribose 5-phosphate and 0.6 mM with ribulose 1,5-diphosphate.

3. 3. Dithioerythritol or light enhanced Mg²⁺-stimulated CO₂ fixation 1–3 fold in the presence of ATP plus ribose 5-phosphate but not ribulose 1,5-diphosphate.

4. 4. These results indicate that Mg²⁺ fluxes in the stroma of the chloroplast could control the activity of the phosphoribulokinase with a lesser effect on the ribulosediphosphate carboxylase. An increase in Mg²⁺ of 6–10 mM in the stroma region of the chloroplast would be enough to activate CO₂ fixation during photosynthesis.

Indoor environmental study and post occupancy evaluation in an office environment

1. 1. The objective of this paper is to investigate the indoor environment from the viewpoint of interaction between physical environment and the human responses. The field survey has been conducted over 1 year.

2. 2. A continuous measurement has been carried out for 1 week and distribution of variables have been measured for 1 day.

3. 3. The attitude of workers was investigated by a questionnaire.

4. 4. As the result, average luminance represented more than 1000 lx in the new building, in contrast with less tha 300 lx in the existing building.

5. 5. There was a significant difference of the occupants' response to the light environment between the two buildings.

6. 6. Measured thermal conditions are on the edge of the ASHRAE comfort envelope in summer, and in the neighborhood of the lower dry limit of the envelope in spring.

7. 7. The occupants' evaluations were remarkably changed before and after the moving. The office environment is better than that of the factory.

Electrical skin resistance and rectal temperature changes in resting human subjects exposed to heat

1. 1.|Fourteen male volunteers were examined under passive heating.

2. 2.|Electrical skin resistance (ESR) and rectal temperature (T_re) were measured during the whole period of exposure.

3. 3.|It was found that:

• —|ESR decreases rapidly with increasing air temperature. Assuming an exponential curve yields a mean time constant of 14 min.

• —|There is a correlation between the individual ESR time constants and T_re increases (r = 0.695, P < 0.005).

• —|Additional changes of ESR were noted in 8 subjects at a constant air temperature of 42°C.

4. 4.|It is concluded that ESR may be a useful indicator of the sweating response of the human thermoregulatory system during exogenous heat load.

Acetylene reduction with physiological electron donors by extracts and particulate fractions from nitrogen-fixing Azotobacter chroococcum

1. 1. Preparations were obtained from Azotobacter chroococcum which reduced acetylene to ethylene using physiological electron donors instead of sodium dithionite. These preparations fell into two categories: those which required catalytic amounts of benzyl viologen for acetylene reduction and those that did not.

2. 2. Acetylene reduction without benzyl viologen or sodium dithionite was observed only with particles that sedimented at 40 000 × g after disrupting bacteria in the French press or with preparations obtained by disrupting bacteria protected by a mixture of defatted bovine serum albumin-Ficoll-MgCl₂ with liquid N₂; supernatant fractions required benzyl viologen for acetylene reduction.

3. 3. Added ATP inhibited acetylene reduction by large particles; ATP and MgCl₂ were necessary for maximum acetylene reduction with bovine serum albumin-protected preparations.

4. 4. NADH and carbon substrates acted as electron donors but H₂ did not; NAD⁺ was necessary for maximum acetylene reduction with carbon substrates.

5. 5. Anaerobic conditions were necessary for maximum acetylene reduction in all cases.

Investigations on the surveys of office environments and the total evaluation method—IAQ-Index

1. 1. A comprehensive study was made to establish evaluation methods for better office environments.

2. 2. In our study, measurements of thermal, acoustic, lighting, airflow and air quality conditions in indoor environments were made as well as questionnaire to the occupants on the evaluations of indoor environments and the feeling of fatigue.

3. 3. We also made an attempt to rate the evaluation value based on concepts and standards to evaluate totally office environments in a view point of “the office environments where people can work healthy and vigorously”. The evaluation values were called IAQ-index.

Electron transport particles released upon lysis of spheroplasts of Escherichia coli B by Brij 58

The non-ionic detergent, Brij 58, has been shown to specifically lyse the cytoplasmic membrane of Escherichia coli. This communication examines the electron transport system in membrane fractions prepared from such lysates and compares this system to those prepared by mechanical procedures.

1. 1. The particulate fraction contained all of the respiratory carriers demonstrable in whole cells in essentially the same ratios although enriched by 3–5-fold over their concentration in whole cells.

2. 2. Succinate, formate, and NADH but not glutamate, malate, or dihydroorotate were actively oxidized by the particulate fraction.

3. 3. With the exception of the formate oxidase system which appeared to utilize a system bypassing flavoprotein and cytochrome b₁, the other enzymatic activities appeared to function primarily through normal electron transport routes.

4. 4. The NADH oxidase and succinoxidase systems were sensitive to inhibition by 2-n-heptyl-4-hydroxyquinoline-N-oxide and antimycin A. The results suggest that these inhibitors function at the level of cytochrome b₁.

5. 5. All three activities were sensitive to complete inhibition by CN⁻.

6. 6. The results obtained from inhibitor studies coupled with the results obtained from examination of steady state, anaerobic, and chemical reduction of the respiratory pigments permitted a scheme for electron flow to be proposed.

Polar bear thermoregulation: Effect of oil on the insulative properties of fur

1. 1.|Oil caused a substantial decrease in the insulative value of polar bear (Ursus maritimus) pelts measured in vitro.

2. 2.|Following oil contamination the calm air heat transfer coefficient increased by a factor of 2 to 5: the wind coefficient averaged 290% greater and the solar utilization increased by 55%.

3. 3.|Conductance through oil-covered furs remained high at winter temperatures (T_a = 0.6°C) but decreased with time at summer temperatures (T_a = 24.7°C).

4. 4.|The most viscous of the three oils tested had a more consistently negative effect on insulation.

Application of fuzzy control to air conditioning environment

1. 1. The application method of fuzzy control to air conditioning environment is shown taking home and railcar air conditioners as an example.

2. 2. The control system developed by us includes the capacity control as the basic application example, the control to determine a set temperature, and that to correct automatically the set temperature.

Thermal gradients in the oesophagus of man during exercise and passive warming

1. 1.|Oesophageal temperature (T_es) has been recorded at four vertical levels in the oesophagus of human subjects during exercise and during passive body heating.

2. 2.|The temperature increased with depth.

3. 3.|The rate of increase in T_es during exercise was greatest at the level of the diaphragm.

4. 4.|During passive heating of the body T_es increased most rapidly, and with shortest latency time, at the level adjacent to the left atrium of the heart.

5. 5.|During exercise, when breathing is deeper than at rest, T_es should be measured at a deeper level than that which is used during passive heating.

Time-resolved respirometry: Equations for the simultaneous measurement of all respiratory gases and the calibration of oxygen consumption using variable inert gas flow rates

1. 1.|Respiratory gas exchange equations for simultaneous time-resolved determination of oxygen consumption, carbon dioxide production, and evaporative water loss are presented.

2. 2.|The overall accuracy of time-resolved respiratory gas analysis may be tested by using variable rates of injection of an inert gas to simulate a variable rate of oxygen consumption.

TLV in cold environments

1. 1. The risks encountered during cold exposure are general body cooling or local cooling of parts of th body.

2. 2. Measures of cold stress must account for the effects of climate, clothing and metabolic heat production on heat balance.

3. 3. The combinaed effect of air temperature, mean radiant temperature, humidity and air velocity determines the cooling power of the environment.

4. 4. The cooling power can be easily converted into a required insulation value (IREQ) for whole body heat balance.

5. 5. Extensive cooling of hands and feet may be a limiting factor, even when sufficient total insulation is provided. In addition the cooling effect of wind on unprotected skin must be considered.

6. 6. Recommendation regarding acceptable exposures can be expressed as lowest ambient temperatures and time limits as function of available protection and activity level, with due attention to both general and local effects.