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1.
Srivastava Arvind K., Jaffe Julian J. and Lambert Roger A. 1985. Phosphatidylethanolamine synthesis in adult Dirofilaria immitis females. International Journal for Parasitology15: 429–433. Adult Dirofiliaria immitis females were found able to synthesize phosphatidylethanolamine (PE) by way of the following three pathways: (1) phosphorylethanolamine, cytidine diphosphoethanolamine and 1,2-diacylglycerol; (2) decarboxylation of phosphatidylserine (PS); and (3) direct exchange of ethanolamine for choline or serine in preformed phosphatidylcholine or PS. The latter two pathways were confined to the paniculate fraction of worm homogenates. Under stated assay conditions, the respective rates of PE formation by way of these pathways in the order given were around 250, 8500 and 2–3 pmol min?1 mg?1 protein. 相似文献
2.
Srivastava A. K. and Jaffe J. J. 1987. Phosphatidylinositol, phosphatidylglycerol, and cardiolipin synthesis in adult Dirofilaria immitis females. International Journal for Parasitology17:917–920. The pathways leading to the formation of phosphatidylinositol (PI), phosphatidylglycerol (PG) and cardiolipin (CL) in adult Dirofilaria immitis females were investigated. PI was synthesized by both de novo as well as via base exchange pathway in the worms. Under specified assay conditions, the respective rates of PI formation by way of these pathways in crude homogenates of the worms in the order given were around 3.0 and 0.75 nmol min−1 mg−1 protein. PG synthesizing activity in the worms was mainly associated with the particulate fractions and the rate of formation by these fractions was around 1.5 nmol min−1mg−1 protein. The worms were unable to synthesize CL by the pathway found in mammals. 相似文献
3.
《Plant Science Letters》1982,24(1):91-95
Diacyglyceryl-O-4′-(N,N,N-trimethyl) homoserine (DGTS), a lipid which is typical of Ochromonas (Chrysophyceae) and Chlamydomonas (Chlorophyceae), has also been detected in Polytoma and Fritschiella (Chlorophyceae), but not in Chlorella and Prototheca (Chlorophyceae), Euglena (Euglenophyceae) and Fucus (Phaeophyceae). The large contents of DGTS in Ochromonas and Chlamydomonas parallel low contents of phosphatidyl choline (PC). From this it is suggested that DGTS may substitute for PC in these algae. DGTS is formed by algae of different taxonomic positions and by both photosynthesizing and non-photosynthesizing species. A correlation between the presence of DGTS and cellular organization has not been detected. 相似文献
4.
5.
《Journal of Fermentation and Bioengineering》1990,69(1):23-25
The acyl exchange of phospholipids with lipases was investigated. The lipase from Rhizopus delemar catalyzed the acyl exchange reaction between various phospholipids and fatty acids. When we incubated 1,2-dipalmitoyl-sn-glycero-3-phosphatidyl choline (DPPC) and oleic acid with lipase from R. delemar, the yield of diacyl phosphatidyl choline (PC) was 25% and the fatty acid composition of the converted PC was an oleic acid content of 25% and a palmitic acid content of 75%. This reaction exhibited 1-positional specificity. Three industrial lipases from Rhizopus sp., Mucor javanicus, and Candida cylindracea had the activity of the acyl exchange of phosphatidyl choline. The lipase from R. sp. gave the best result. 相似文献
6.
Meningoencephalitis was produced in albino mice by intranasal inoculation of Hartmanella culbertsoni. In the infected brain phosphatidyl choline (PC), phosphatidyl ethanolamine + phosphatidyl serine (PE + PS), sphingomyelin and cholesterol registered decrease, lysophospholipids and free fatty acids accumulated, and part of the cholesterol was esterified. Phospholipase A (EC 3.1.1.4) and sphingomyelinase (EC 3.1.4.12) were elaborated in the postmitochondrial supernatant fraction. The levels of lipid peroxides, in brain as well as the capacity of brain homogenates to form lipid peroxides in vitro, was higher in the infected animals as compared to the uninfected. The activities of succinate dehydrogenase (EC 1.3.99.1) and cytochrome c oxidase (EC 1.9.3.1) in the mitochondrial fraction of the infected brain decreased while adenosine triphosphatase (EC 3.6.1.3) was stimulated. Addition of cytosol fraction (105,000g supernatant) of infected brain to the mitochondrial fraction of uninfected brain caused inhibition of succinate dehydrogenase and cytochrome c oxidase and stimulation of adenosine triphosphatase. This shows that some toxic substance(s) which inhibits mitochondrial function in brain is produced in the cytosol during infection. This inhibitor was nondialyzable and heat stable. 相似文献
7.
Otto Geiger Isabel M. López-LaraChristian Sohlenkamp 《Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids》2013,1831(3):503-513
Phosphatidylcholine (PC) is the major membrane-forming phospholipid in eukaryotes and is estimated to be present in about 15% of the domain Bacteria. Usually, PC can be synthesized in bacteria by either of two pathways, the phospholipid N-methylation (Pmt) pathway or the phosphatidylcholine synthase (Pcs) pathway. The three subsequent enzymatic methylations of phosphatidylethanolamine are performed by a single phospholipid N-methyltransferase in some bacteria whereas other bacteria possess multiple phospholipid N-methyltransferases each one performing one or several distinct methylation steps. Phosphatidylcholine synthase condenses choline directly with CDP-diacylglycerol to form CMP and PC. Like in eukaryotes, bacterial PC also functions as a biosynthetic intermediate during the formation of other biomolecules such as choline, diacylglycerol, or diacylglycerol-based phosphorus-free membrane lipids. Bacterial PC may serve as a specific recognition molecule but it affects the physicochemical properties of bacterial membranes as well. This article is part of a Special Issue entitled Phospholipids and Phospholipid Metabolism. 相似文献
8.
S Turunen 《Journal of insect physiology》1974,20(7):1257-1269
Fatty acids of adult Pieris brassicae and the incorporation of dietary linolenic acid-1-14C into adult (and egg) lipids were analysed 1 and 9 days after ecdysis. Females grown on a leaf diet retained palmitic, palmitoleic, and oleic acids but lost linoleic and linolenic acids during adult life, while males utilized their fatty acids more evenly. On an artificial diet both sexes retained palmitic acid but utilized palmitoleic and oleic acids. In both cases females laid eggs with a high palmitic and oleic acid content.Analysis of thorax flight muscles (artificial diet) revealed that 67·9% of the lipids in 1-day females and 83·6% in 9-day females was phospholipid (PL). During adult life linolenic acid increased in thorax neutral lipids (NL) from 14·6 to 20·0% in females and from 18·5 to 30·0% in males. Males incorporated more linolenic acid-1-14C especially in fat body and flight muscle PL than females. The majority of this was recovered from phosphatidyl cholines (PTC) in 1-day adults whereas in 9-day adults phosphatidyl ethanolamines (PTE) and another compound, most likely cardiolipin, contained more label (29·0% in PTC, 33·1% in PTE, 34·9% in cardiolipin, and 2·0% in sphingomyelin in the thorax of females). The females also incorporated the label into egg lipids (42·2% in PL, 57·8% in NL). There was recovered from PTC 54·5% of the label in egg PL.Most of the label in thorax NL was found to be in free fatty acids (FFA). The label disappeared from triglycerides during adult life and tended to accumulate in FFA (82·7% in 9-day females) while in diglycerides the label did not vary during adult life (17·2% in 9-day post-emergence females). PTC apparently is a fairly labile PL type which is utilized in muscle whereas PTE and cardiolipin may be more structural in function and accumulated more label from linolenic acid with increasing adult age. Linolenic acid, then, essentially is a structural fatty acid and its rôle appears to be mainly in the structures of flight muscle membranes and organelles. 相似文献
9.
Chromatographically pure hexadecylphosphocholine, -(N,N-dimethyl)-ethanolamine, -(N-N-methyl)-ethanolamine and -ethanolamine have been synthesized. Aqueous solutions of these phospholipids have been prepared for the purpose of measuring their dielectric spectra. Micellar solutions appropriate for the dielectric studies were obtained with the choline and the (N,N-dimethyl)-ethanolamine head groups.The dielectric spectra of these phospholipid/water systems are evaluated in terms of phenomenologically introduced sum of Cole-Cole relaxation functions and also on the basis of a model relaxation function which has regard to internal depolarizing fields of the colloidal solutions. Parameters reflecting the motions of the dipolar head groups and of the hydration water molecules of the synthetic monoalkyl phosphodiesters are discussed and are compared with those for egg lysolecithin.The mobility of the dipolar phospholipid head groups and the number of influenced water molecules per zwitterion decreases when changing from lysolecithin to hexadecylphosphocholine and further to hexadecylphospho-(N,N-dimethyl)-ethanolamine, while the relaxation time of the hydration water increases. These results indicate the micellar surface to get less porous within the above series of lipids. 相似文献
10.
Differential regulation of the N-methyl transferases responsible for phosphatidylcholine synthesis in Saccharomyces cerevisiae 总被引:25,自引:0,他引:25
The enzymes catalyzing the conversion of phosphatidylethanolamine to phosphatidylcholine were assayed by measuring the incorporation of label from [14C-CH3]-S-adenosyl-methionine into the endogenous phospholipids of particulate, cell-free preparations from S. cerevisiae grown in the presence of N-methylethanolamine, N,N-dimethylethanolamine, or choline. The results indicate that each base in the growth medium results in reduced levels of all the N-methyltransferase activity involved in the formation of the phosphatidyl ester of the given base. By following the conversion of exogenous [32P]-phosphatidyldimethylethanolamine to [32P]-phosphatidylcholine it has been shown that the activity of the third methyl transfer is 90% lower in particles prepared from choline grown cells than in particles prepared from cells grown without choline. The results suggest that there are at least two enzymes involved in the conversion of phosphatidylethanolamine to phosphatidylcholine and that their levels can be regulated individually.Supplementing the growth medium with any of the three methylated aminoethanols results in markedly increased cellular levels of their corresponding phosphatidyl esters and decreased levels of the precursor phosphatidyl esters. The fatty acid composition of phosphatidylcholine also changes when the medium is supplemented with choline suggesting that the proportions of the molecular species of this phosphatide depends on whether synthesis is via methylation of phosphatidylethanolamino or from the supplemented aminoethanol. 相似文献
11.
《Bioorganic & medicinal chemistry》2014,22(24):6933-6944
Acid ceramidase (ACDase) is being recognized as a therapeutic target for cancer. B13 represents a moderate inhibitor of ACDase. The present study concentrates on the lysosomal targeting of B13 via its N,N-dimethylglycine (DMG) esters (DMG-B13 prodrugs). Novel analogs, the isomeric mono-DMG-B13, LCL522 (3-O-DMG-B13·HCl) and LCL596 (1-O-DMG-B13·HCl) and di-DMG-B13, LCL521 (1,3-O, O-DMG-B13·2HCl) conjugates, were designed and synthesized through N,N-dimethyl glycine (DMG) esterification of the hydroxyl groups of B13. In MCF7 cells, DMG-B13 prodrugs were efficiently metabolized to B13. The early inhibitory effect of DMG-B13 prodrugs on cellular ceramidases was ACDase specific by their lysosomal targeting. The corresponding dramatic decrease of cellular Sph (80–97% Control/1 h) by DMG-B13 prodrugs was mainly from the inhibition of the lysosomal ACDase. 相似文献
12.
The effect of lidocaine on [3H]choline uptake and the incorporation of label into phosphatidylcholine (PC) in human monocyte-like U937 cells was investigated. Lidocaine inhibited the rate of choline uptake in a dose-dependent manner; at 3·2 mM it resulted in a drastic reduction, by as much as 65 per cent (n = 10; p < 0·0005) or 55 per cent (n = 10; p < 0·0006) in a 3- or 6-h incubation, respectively. Lidocaine also decreased the rate of choline incorporation into PC in a dose-dependent manner. At the highest dose, nearly 70 per cent or 45 per cent reduction was seen in a 3- or 6-h incubation, respectively. Analysis of choline-containing metabolites showed that the major label association with phosphocholine and PC was reduced to a similar extent which was also parallel to the inhibition of choline uptake. At 3·2 mM lidocaine, the reduction of choline uptake was shown to follow a competitive inhibition. In the case of [3H] choline incorporation into PC, the inhibitory pattern was shown to be of a mixed type. The pulse-chase study dissecting the effect on choline metabolism from that on total choline uptake indicated that lidocaine exerted an additionally inhibitory effect on intracellular choline metabolism into PC. In a separate protocol in which the labelled cells were first allowed to be chased until 3H-incorporation into PC reached a steady state, lidocaine no longer showed any effect. These results seem to exclude the possibility of enhanced PC breakdown and further suggest that the main inhibitory effect is on the CDP-choline pathway for PC biosynthesis. After a 3-h treatment, CTP: cholinephosphate cytidylyltransferase (CYT) in both the cytosolic and microsomal fractions was inhibited by approximately 20 per cent, while choline kinase (CK) and choline phosphotransferase (CPT) remain relatively unchanged. There was no evidence for translocation of CYT between cytosol and microsomes. Taken together, we have demonstrated a dual inhibitory function of lidocaine which inhibits PC biosynthesis in addition to its ability to block choline uptake profoundly in U937 cells. 相似文献
13.
Phosphatidyl choline (PC) is synthesised in wheat (Triticum aestivum L. cv. Flanders) aleurone tissue during early germination when new endomembranes are being formed. Although gibberellic acid does not ostensibly affect PC levels, it inhibits the incorporation of choline and differentially and specifically modulates the turnover of the N-methyl and methylene carbons of the choline headgroup of PC. Gibberellic acid has no effect on turnover of the phosphate moiety of either PC or the other major phosphatides. The possible biological importance of the findings is discussed.Abbreviations ER
endoplasmic reticulum
- GA
gibberellin
- GA3
gibberellic acid
- PA
phosphatidic acid
- PC
phosphatidyl choline
- PE
phosphatidyl ethanolamine
- PG
phosphatidyl glycerol
- PI
phosphatidyl inositol
- t1/2
half-life 相似文献
14.
A.C. Thompson Frank M. Davis R.D. Henson R.C. Gueldner P.A. Hedin C.A. Henderson 《Journal of insect physiology》1973,19(9):1817-1823
The lipids of the adults and of several immature stages of the southwestern corn borer, Diatraea grandiosella, were studied after they were fed natural corn stalks or artificial diets. Linoleic acid (18:2) was the major fatty acid of the neutral lipids in both the natural and the artificial diets, but aleic acid (18:1) was the principal neutral lipid in all insect stages. Also, linoleic acid and oleic acid were the principal acids in the insect phospholipids of all stages. The content of linoleic acid in the natural diet was also high, but that in the artificial diet appeared to be much too low for insect requirements. Phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) were the major phospholipids in all growth stages. Thus, in larvae diapausing in the field, the unsaturated fatty acid content of PC was 59·3 per cent, primarily 16:1 and 18:1, and PE was 87·4 per cent, primarily 18:1, 18:2, and 18:3, and the fatty acids in the number 1- and 2-positions of PC were 53·6 and 97·2 per cent unsaturated, respectively. The haemolymph of diapausing southwestern corn borer larvae contained primarily glycerides but also had some PC and PE. Fat body from diapausing larvae contained primarily 16:0, 16:1, and 18:1 in a ratio of 1 : 1 : 2. Thus lipids of the southwestern corn borer do not reflect dietary lipids as closely as do other insects studied. 相似文献
15.
Reiji Kannagi Kinya Koizumi Sachiko Hata-Tanoue Tohru Masuda 《Biochemical and biophysical research communications》1980,96(2):711-718
Rabbit platelets rapidly incorporated methyl groups of [3H] methionine to phosphatidylcholine (PC). Rabbit platelets also incorporated [3H]choline to PC, but the rate of incorporation was far lower than that of [3H]methionine. Further fractionation of labeled PC revealed that a considerable amount of arachidonyl PC was synthesized via the N-methylation pathway. Thrombin stimulation resulted in a release of arachidonic acid from PC, and not from phosphatidylethanolamine (PE). These observations suggest that the N-methylation pathway plays an important role in the intracellular mobilization of arachidonic acid from the PE fraction to the PC fraction, this fraction being more sensitive to the hydrolysis with phospholipase A2 during platelet activation. 相似文献
16.
Claudia K. Herrmann Lucas Bukata Luciano Melli M. Ines Marchesini Julio J. Caramelo Diego J. Comerci 《Journal of bacteriology》2013,195(3):493-501
Phosphatidylcholine (PC), a common phospholipid of the eukaryotic cell membrane, is present in the cell envelope of the intracellular pathogen Brucella abortus, the etiological agent of bovine brucellosis. In this pathogen, the biosynthesis of PC proceeds mainly through the phosphatidylcholine synthase pathway; hence, it relies on the presence of choline in the milieu. These observations imply that B. abortus encodes an as-yet-unknown choline uptake system. Taking advantage of the requirement of choline uptake for PC synthesis, we devised a method that allowed us to identify a homologue of ChoX, the high-affinity periplasmic binding protein of the ABC transporter ChoXWV. Disruption of the choX gene completely abrogated PC synthesis at low choline concentrations in the medium, thus indicating that it is a high-affinity transporter needed for PC synthesis via the PC synthase (PCS) pathway. However, the synthesis of PC was restored when the mutant was incubated in media with higher choline concentrations, suggesting the presence of an alternative low-affinity choline uptake activity. By means of a fluorescence-based equilibrium-binding assay and using the kinetics of radiolabeled choline uptake, we show that ChoX binds choline with an extremely high affinity, and we also demonstrate that its activity is inhibited by increasing choline concentrations. Cell infection assays indicate that ChoX activity is required during the first phase of B. abortus intracellular traffic, suggesting that choline concentrations in the early and intermediate Brucella-containing vacuoles are limited. Altogether, these results suggest that choline transport and PC synthesis are strictly regulated in B. abortus. 相似文献
17.
The thermal decomposition of methyl 4,6-O-benzylidene-2,3-di-O-[(methylthio)-thiocarbonyl]-α-d-glucopyranoside afforded methyl 4,6-O-benzylidene-2-thio-α-d-mannopyranoside 3-O,2-S-(S,S-dimethyl trithioorthocarbonate) and methyl 4,6-O-benzylidene-3-thio-α-d-allopyranoside 2-O,3-S-(S,S-dimethyl trithioorthocarbonate) in good yield. This decomposition can be generalized to 1,3-diols derived from sugars. Thus methyl 2,3-di-O-methyl-4,6-di-O-[(methylthio)thiocarbonyl]-α-d-glucopyranoside afforded in the same way the corresponding trithioorthocarbonates, following a regioselective process. The structures of these trithioorthocarbonates are confirmed by spectral and chemical proofs. 相似文献
18.
19.
The phospholipid composition of Micrococcus denitrificans was unusual in that phosphatidyl choline (PC) was a major phospholipid (30.9%). Other phospholipids were phosphatidyl glycerol (PG, 52.4%), phosphatidyl ethanolamine (PE, 5.8%), an unknown phospholipid (5.3%), cardiolipin (CL, 3.2%), phosphatidyl dimethylethanolamine (PDME, 0.9%), phosphatidyl monomethylethanolamine (PMME, 0.6%), phosphatidyl serine (PS, 0.5%), and phosphatidic acid (0.4%). Kinetics of 32P incorporation suggested that PC was formed by the successive methylations of PE. Pulse-chase experiments with pulses of 32P or acetate-1-14C to exponentially growing cells showed loss of isotopes from PMME, PDME, PS, and CL with biphasic kinetics suggesting the same type of multiple pools of these lipids as proposed in other bacteria. The major phospholipids, PC, PG, and PE, were metabolically stable under these conditions. The fatty acids isolated from the complex lipids were also unusual in being a simple mixture of seven fatty acids with oleic acid representing 86% of the total. Few free fatty acids and no non-extractable fatty acids associated with the cell wall or membrane were found. 相似文献
20.
The effect of (0·05 ng ml−1 and 0·1 ng ml−1) TNFα on the phospholipid metabolism of Tetrahymena pyriformis was studied. The amount of phosphatidyl choline (PC), phosphatidyl inositol (PI), phosphatidic acid (PA), phosphatidyl ethanolamine (PE), diacylglycerol (DAG), arachidonic acid (AA) and ceramide was higher, but the phosphatidyl inositol 4 phosphate (PIP) and phosphatidyl inositol bis-phosphate (PIP2) as well, as sphingomyelin (SM) content was lower in TNFα-treated cells than in the controls. In the culture medium (secreted forms) this situation was reversed. There were differences in the results gained by incorporation of [3H]-palmitic acid or 32P into the phospholipids. To control the functional effects of TNFα in Tetrahymena, the rate of cell division, the condensation of chromatin, the viability of cells and morphometrical values have been studied. The cytokine reduced cell growth, altered morphometric indices and increased chromatin condensation, however cell viability was not influenced. The results demonstrate the effects of TNFα at a low level of evolution, what is realized by changes in the phospolipid metabolism participating in signalling pathways. © 1998 John Wiley & Sons, Ltd. 相似文献