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1.
P Sepulveda K W Jackson J Tang 《Biochemical and biophysical research communications》1975,63(4):1106-1112
The amino acid sequences near the amino termini of human pepsin (34 residues) and gastricsin (24 residues) and the acid protease from (27 residues) have been determined using automated Edman degradation. From these results three additional observations were made. First, two structural variants have been observed for human gastricsin and for the protease. Both cases are apparently genetic in origin. Second, a stretch of sequence in the protease, residues 14 to 26, is highly homologous to the known sequence of porcine pepsin at the region of residues 11 to 23. Third, the sequences of the NH2-terminal region of human pepsin and gastrisin are homologous. 相似文献
2.
Anne Lecroisey André De Wolf Borivoj Keil 《Biochemical and biophysical research communications》1980,94(4):1261-1265
The sequence of 32 N-terminal amino acid residues of the collagenase from the larvae Hypoderma lineatum of mol. weight 24000 is homologous with the sequences of pancreatic proteinases. This analysis confirms the hypothesis of similarity made on the basis of amino acid composition, physico-chemical properties and inhibition studies. It is proposed that within the trypsin-like family exists a group of eucaryote collagenases with digestive rather than morphogenic function which cleave specifically native collagen at from its N-terminus. 相似文献
3.
A tetragonal crystal form of horse spleen apoferritin and its relationship to the cubic modification
Horse spleen apoferritin has been crystallized as tetragonal plates and needles with a unit cell with . The space group is P4212 and the unit cell contains two molecules in a pseudo-body-centred arrangement. The intensity distributions and calculated rotation functions of tetragonal and cubic crystals have been compared. The symmetry of the diffraction patterns from cubic crystals indicates that the molecules have 432 symmetry with their 4-fold axes lying along the cube axes. In the tetragonal crystals one molecular 4-fold axis lies parallel to c, the unique axis, while the rest of the molecular point symmetry is not used by the lattice. Instead the remaining 4-fold axes of the two molecules, which lie in planes perpendicular to c, are rotated ± 17.5 ° with respect to the tetragonal a axis. The finding that apoferritin reassembled from subunits can be crystallized in both tetragonal and cubic forms confirms its conformational similarity to native molecules. 相似文献
4.
Porcine ferritin and apoferritin were purified to a greater degree of homogeneity than has been reported previously. Porcine ferritin was insoluble in the absence of a reducing agent, possessed a high content of iron, with an average ratio of ~2.5, and contained almost no detectable endogenous apoferritin. The amino acid composition, ultraviolet-absorption spectrum, and ultraviolet-circular dichroism spectrum of porcine apoferritin are very similar to the respective parameters of equine apoferritin. The native and subunit molecular weights of porcine apoferritin are 503,000 and 20,000, respectively. 相似文献
5.
M Tanaka M Haniu K T Yasunobu 《Biochemical and biophysical research communications》1975,66(2):639-644
The amino acid sequence of a group II flavodoxin, the flavodoxin has been determined. The FMN-redox protein was shown to exist as a single polypeptide chain and to contain 179 amino acids. Despite the rather low amino acid sequence homology with the other flavodoxins sequenced, it is concluded that sequences of the group I and group II flavodoxins are homologous. The major differences between the group I and group II flavodoxins appears to be a lengthening in the C-terminal region in the group II flavodoxins. 相似文献
6.
The amino acid sequences of pyridoxal-binding tetrapeptide and the NH2-terminal portion of aspartate transaminase from B were analyzed and compared with those of the corresponding parts of the cytosolic and mitochondrial isozymes from pig heart. After borohydride reduction and chymotryptic digestion of the enzyme, a pyridoxal-containing peptide was isolated, showing the sequence, Ser-Lys(Pxy)-Asn-Phe, identical with that of the cytosolic isozyme. The NH2-terminal sequence was determined up to 33 residues with a liquid phase sequence analyzer. Nearly the same degree of homology was observed among the NH2-terminal sequences of the three aspartate transaminases. 相似文献
7.
A 22-residue peptide toxin from the venomous marine snail Conus geographus (L.) was found to have a most unusual amino acid composition: Lys4, Arg3, , Asx2, Glx2, Thr, Ala, plus three residues of trans-4-hydroxyproline. Absence of Gly and Pro indicates that the hydroxyproline must be in sequences different from those in which hydroxyproline occurs in collagen and other proteins. 相似文献
8.
Proline inhibition of pyrroline-5-carboxylate reductase: differences in enzymes obtained from animal and tissue culture sources 总被引:3,自引:0,他引:3
D Valle S J Downing J M Phang 《Biochemical and biophysical research communications》1973,54(4):1418-1424
The complete amino acid sequence for the 148 amino acid flavodoxin from is presented. This is the first flavoenzyme for which both the complete amino acid sequence and a 2.5 Å resolution x-ray diffraction structure are now known. The position of some important residues in the binding of FMN are given. The D. vulgaris sequence is compared with other published flavodoxin sequences. 相似文献
9.
DNA replication in a polymerase I deficient mutant and the identification of DNA polymerases II and 3 in Bacillus subtilis 总被引:6,自引:0,他引:6
A T Ganesan C O Yehle C C Yu 《Biochemical and biophysical research communications》1973,50(1):155-163
The partial amino acid sequences at the amino terminal of prothrombin and the intermediates of activation have been determined. These data indicate that the products of the first step of activation, whether derived from the action of factor Xa or thrombin, are identical. The data also show that the activation of prothrombin proceeds by the sequential cleavage of the amino terminal region of prothrombin and the intermediates, and confirm the mechanism of prothrombin activation as: NH2-Prothrombin-COOH NH2-Intermediate 3 + Intermediate 1-COOH; NH2-Intermediate 1-COOH NH2-Intermediate 4 + Intermediate 2-COOH; NH2-Intermediate 2-COOH NH2-A chain α-thrombin -S-S-B chain α-thrombin-COOH.Previous reports from this laboratory have demonstrated that the activation of prothrombin proceeds through several single-chain intermediates prior to the appearance of thrombin activity. (1) Subsequent studies have sequence of the prothrombin molecule can be deduced from the sequences of its activation intermediates and we are continuing our studies toward this goal. 相似文献
10.
Toshifumi Takao Noriko Tominaga Yasutsugu Shimonishi Saburo Hara Takashi Inoue Akio Miyama 《Biochemical and biophysical research communications》1984,125(3):845-851
A heat-stable enterotoxin was isolated and purified from the culture supernatant of by reversed-phase high-performance liquid chromatography. The amino acid sequence of the purified toxin was determined to be as follows: Gln-Ala-Cys(X)-Asp-Pro-Pro-Ser-Pro-Pro-Ala-Glu-Val-Ser-Ser-Asp-Trp-Asp-Cys-Cys-Asp-Val-Cys-Cys-Asn-Pro-Ala-Cys-Ala-Gly-Cys (X: not determined). The C-terminal sequence containing 6 half-cystine residues was highly homologous to that of heat-stable enterotoxin of enterotoxigenic 相似文献
11.
Stimulation by interferon of induction of differentiation of human promyelocytic leukemia cells 总被引:3,自引:0,他引:3
F1-ATPase was isolated from yeast . The constituent subunits 1 and 2 were purified by gel permeation chromatography, and their amino acid compositions determined. Both subunits have a similar composition except for cystine, methionine, leucine, histidine, and tryptophan. When F1 is treated for three hours with 5′-p-[3H]fluorosulfonylbenzoyl adenosine in dimethylsulfoxide, 90% of the activity is lost. Disc gel electrophoresis of the modified complex showed that over 90% of the label was associated with subunit 2. A labelled peptide from a digest of subunit 2 was isolated and sequenced. It had the following amino acid sequence: His-Try1-Asp-Val-Ala-Ser-Lys-Val-Gln-Glu, whereby Tyr1 is the modified amino acid residue. This sequence shows homology to other sequences obtained from maize, beef heart, and F1-ATPases. 相似文献
12.
F. Guerlesquin G. Bovier-Lapierre M. Bruschi 《Biochemical and biophysical research communications》1982,105(2):530-538
An acidic cytochrome c (Pi = 4.8) has been purified from Norway. Its molecular weight was estimated to be 26,000 but a monomeric form of 13,500 molecular weight has been obtained. The comparison of its amino acid composition and N terminal sequence has characterized this cytochrome as a new cytochrome, different from cytochrome c3 (Mr 13,000) and cytochrome c553(550) studied in the same organism. Its optical spectrum was similar to cytochrome c3 (Mr 13,000) accordingly it has 4 haems per subunit. The absence of absorption at 695 nm indicates that two histidine residues are implicated as fifth and sixth ligand for haem iron. This new cytochrome is homologous to the cytochrome C3 (Mr 26,000) previously described for and . 相似文献
13.
Interchain disulfide bridges in ribonuclease BS-1 总被引:3,自引:0,他引:3
RNAase BS-1, a dimeric ribonuclease isolated from bovine seminal plasma, is made up of two identical subunits whose amino acid sequence is homologous to the sequence of bovine pancreatic RNAase A. The dimeric structure, resistant to denaturating agents, is sensitive to thiol reagents even in the absence of denaturants. The isolation and characterization of a cystine peptide containing two adjacent residues is reported. As the peptide molecular weight is halved after reductive cleavage with dithiothreitol, a structure based on two interchain disulfide bonds between the two adjacent of each subunit is proposed. The singularity of such a structure for a small enzymatic protein is discussed. 相似文献
14.
James B. Howard Thomas Lorsbach Lawrence Que 《Biochemical and biophysical research communications》1976,70(2):582-588
In 80% dimethyl sulfoxide/H2O, ferredoxin FeS clusters can be extruded with benzene thiol. The extruded clusters have an absorption spectra maximum at 458 nm which is characteristic of 4Fe4S centers. The amino terminal sequence of the ferredoxin has 7 of the 8 Cys residues at residue numbers 8, 11, 16, 20, 24, 39 and 42. Except for Cys 24, all of these residues can be correlated to homologous Cys residues in other bacterial ferredoxins. Although two thirds of the first 45 residues are identical to or conservative replacements for the first 43 residues of other bacterial ferredoxins, the insertion of Cys-24 indicates a major change in the environment of one of the two 4Fe4S clusters. 相似文献
15.
Analysis of the primary structure of collagen for the origins of molecular packing 总被引:11,自引:0,他引:11
D J Hulmes A Miller D A Parry K A Piez J Woodhead-Galloway 《Journal of molecular biology》1973,79(1):137-148
The amino acid sequence in the triplet region of the α1 chain of collagen was analyzed for complementary relationships that would explain the stagger of multiples of 670 Å between the rod-like molecules in the fibril. The analysis was done by moving the sequence of 1011 amino acids past itself and scoring for complementarity between opposing amino acids allowing a range of ±2 to 3 residues. It was found that interactions between amino acids of opposite charge and between large hydrophobic amino acids in the overlapping region between two chains are maximal when the chains are staggered by 0D, 1D, 2D, 3D and 4D, where D = 234 ± 1 residues. The residue repeat derived from this value is 2.86 ± 0.02 Å. The existence of a D separation between interacting residues was shown to be reflected in the actual distribution of large hydrophobic amino acids. Surprisingly, the distribution approximates the pattern ()5() repeated over 4.4D intervals. The regularity may arise from structural constraints imposed by super-coiling. The distribution of charged residues is less regular and does not show a well-defined periodicity. However, positively-charged residues tend to be near negatively-charged residues, allowing intramolecular charge neutralization as well as strong intermolecular charge interactions at 0D. 相似文献
16.
T G Hayes 《Biochemical and biophysical research communications》1980,95(2):872-879
The Chou-Fasman method for calculating the secondary structure of and interferons from their amino acid sequences predicts several regions of homologous structure in the two interferons. Those areas tend to be located in the middle and C- terminal ends of the molecules. Total predicted content of α helix is 55% for interferon and 36% for interferon. Predicted β-pleated sheet residues total 33% for and 16% for . 相似文献
17.
Tyrosyl-tRNA synthetase from Bacillus stearothermophilus is a dimeric molecule of approximately 90,000 Mr. The crystal structure originally reported by Irwin et al. (1976) has been re-interpreted using a new density-modification technique. The reinterpretation is confirmed by the complete amino acid sequence (D. Barker & (G. Winter, personal communication). The structure consists of an amino-terminal domain, a domain containing five α-helices, and a region of 99 amino acids at the carboxyl terminus, which appears to be disordered. The re-interpretation reveals two new α-helices in the domain, and some changes in chain connections. The strands of the β-sheet are in the order A, F, E, B, C, D, with A antiparallel to the others. The arrangement of strands B to F is topologically identical to arrangements found in many other proteins, including the first five strands of the sheet in the NAD-binding domain of the dehydrogenases. Strands B, C, D form a mononucleotide-binding fold.In the complex with tyrosyl adenylate (Rubin & Blow, 1981), an intermediate in the reaction catalysed by the enzyme, the adenine lies near the carboxyl-terminal end of strand F of the β-sheet, with the ribose between the ends of strands B and E. This is similar to the nicotinamide position in dehydrogenases. The tyrosine moiety occupies a pocket at one side of the sheet, close to strands B and C. This tyrosine orientation is quite different from any part of the coenzyme in dehydrogenases. The ends of strands C and D of the sheet are buried, and binding of a nucleotide to the mononucleotide-binding fold formed by strands B, C, D would require a substantial structural change. 相似文献
18.
Bacteriophage G4 has the same and overlapping gene systems as φX174 and together with the A and overlapping gene system (Shaw et al., 1978), 7 of the 11 G4 and φX174 genes are involved in overlaps. The nucleotide differences between G4 and φX174 in the overlapping portions of the A, C and D genes are 23%, 27% and 21%, respectively, compared with 32%, 36% and 34% in the non-overlapping portions of the same genes. The amino acid differences between the G4 and φX174 overlapping B, K and E proteins, are 44%, 39% and 44%, respectively, compared with 28%, 26% and 16% in the regions of genes A, A and C, and D which contain genes B, K and E. These results suggest that the nucleotide sequences of overlapping genes evolve at almost the same rate as in non-overlapping genes, and that this is made possible by a lower amino acid sequence stringency of one of the pairs of proteins. The overlapping and A and gene systems may have originated by taking advantage of a high incidence of T nucleotides in the second codon position to produce a hydrophobic protein and the gene system may have evolved by read-through of the A gene into the B gene. From the nucleotide sequences, other overlapping genes appear to be possible in these bacteriophages. 相似文献
19.
The amino acid sequence of the ferredoxin has been determined. is a blue green algae and is a procaryote. The ferredoxins of the plant-algal type sequenced to date have all been isolated from eucaryotes. The ferredoxin was composed of 98 amino acids arranged in a single polypeptide chain.The sequences of the various procaryote-eucaryote ferredoxins are compared and the differences discussed. 相似文献
20.
F J Stevens F A Westholm N Panagiotopoulos M Schiffer R A Popp A Solomon 《Journal of molecular biology》1981,147(1):185-193
A “naturally occurring” human κI VL dimer, designated Wat, has been isolated and crystallized. Protein Wat consists of two non-covalently bound monomers, each having a molecular weight of ~ 11,500. The monomer subunit is composed of an entire variable region light chain (VL) domain closely homologous to that of the κI Bence Jones protein Roy (Hilschmann &; Craig, 1965) as evidenced from amino acid composition, tryptic peptide map, and sequence analysis. Immunochemical studies substantiated that protein Wat is of the κ chain subgroup κI and lacks the isotypic and allotypic antigenic determinants associated with the κ constant region light chain domain. Two types of crystals of VL dimer Wat were obtained from ammonium sulfate or polyethylene glycol solutions. The type I crystals have unit cell dimensions of , and the space group is hexagonal P62 or P64. The asymmetric unit consists of one VL dimer; the fractional volume of unit cell occupied by solvent is 0.51. The unit cell dimensions of the type II crystals are ; the space group is hexagonal P6122 or P6522. Three variable domains constitute the asymmetric unit of the type II crystals; the fractional value of the solvent (0.52) is compatible with the value obtained for the type I crystals. 相似文献